Job ID = 6368881 SRX = SRX6619582 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-06-16T00:43:01 prefetch.2.10.7: 1) Downloading 'SRR9866027'... 2020-06-16T00:43:01 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:59:49 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:59:49 prefetch.2.10.7: 1) 'SRR9866027' was downloaded successfully 2020-06-16T00:59:50 prefetch.2.10.7: 'SRR9866027' has 0 unresolved dependencies Read 41642076 spots for SRR9866027/SRR9866027.sra Written 41642076 spots for SRR9866027/SRR9866027.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:20:01 41642076 reads; of these: 41642076 (100.00%) were paired; of these: 25442237 (61.10%) aligned concordantly 0 times 13340764 (32.04%) aligned concordantly exactly 1 time 2859075 (6.87%) aligned concordantly >1 times ---- 25442237 pairs aligned concordantly 0 times; of these: 13070384 (51.37%) aligned discordantly 1 time ---- 12371853 pairs aligned 0 times concordantly or discordantly; of these: 24743706 mates make up the pairs; of these: 21310470 (86.12%) aligned 0 times 1142169 (4.62%) aligned exactly 1 time 2291067 (9.26%) aligned >1 times 74.41% overall alignment rate Time searching: 01:20:01 Overall time: 01:20:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 48 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 4266906 / 28690871 = 0.1487 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 11:55:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619582/SRX6619582.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619582/SRX6619582.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619582/SRX6619582.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619582/SRX6619582.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 11:55:00: #1 read tag files... INFO @ Tue, 16 Jun 2020 11:55:00: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 11:55:08: 1000000 INFO @ Tue, 16 Jun 2020 11:55:17: 2000000 INFO @ Tue, 16 Jun 2020 11:55:25: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 11:55:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619582/SRX6619582.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619582/SRX6619582.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619582/SRX6619582.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619582/SRX6619582.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 11:55:30: #1 read tag files... INFO @ Tue, 16 Jun 2020 11:55:30: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 11:55:34: 4000000 INFO @ Tue, 16 Jun 2020 11:55:39: 1000000 INFO @ Tue, 16 Jun 2020 11:55:45: 5000000 INFO @ Tue, 16 Jun 2020 11:55:49: 2000000 INFO @ Tue, 16 Jun 2020 11:55:57: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 11:55:58: 3000000 INFO @ Tue, 16 Jun 2020 11:56:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619582/SRX6619582.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619582/SRX6619582.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619582/SRX6619582.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619582/SRX6619582.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 11:56:00: #1 read tag files... INFO @ Tue, 16 Jun 2020 11:56:00: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 11:56:08: 7000000 INFO @ Tue, 16 Jun 2020 11:56:09: 4000000 INFO @ Tue, 16 Jun 2020 11:56:10: 1000000 INFO @ Tue, 16 Jun 2020 11:56:19: 5000000 INFO @ Tue, 16 Jun 2020 11:56:19: 8000000 INFO @ Tue, 16 Jun 2020 11:56:21: 2000000 INFO @ Tue, 16 Jun 2020 11:56:29: 6000000 INFO @ Tue, 16 Jun 2020 11:56:30: 9000000 INFO @ Tue, 16 Jun 2020 11:56:31: 3000000 INFO @ Tue, 16 Jun 2020 11:56:39: 7000000 INFO @ Tue, 16 Jun 2020 11:56:41: 4000000 INFO @ Tue, 16 Jun 2020 11:56:41: 10000000 INFO @ Tue, 16 Jun 2020 11:56:49: 8000000 INFO @ Tue, 16 Jun 2020 11:56:51: 5000000 INFO @ Tue, 16 Jun 2020 11:56:52: 11000000 INFO @ Tue, 16 Jun 2020 11:56:59: 9000000 INFO @ Tue, 16 Jun 2020 11:57:01: 6000000 INFO @ Tue, 16 Jun 2020 11:57:03: 12000000 INFO @ Tue, 16 Jun 2020 11:57:09: 10000000 INFO @ Tue, 16 Jun 2020 11:57:11: 7000000 INFO @ Tue, 16 Jun 2020 11:57:15: 13000000 INFO @ Tue, 16 Jun 2020 11:57:19: 11000000 INFO @ Tue, 16 Jun 2020 11:57:21: 8000000 INFO @ Tue, 16 Jun 2020 11:57:26: 14000000 INFO @ Tue, 16 Jun 2020 11:57:29: 12000000 INFO @ Tue, 16 Jun 2020 11:57:31: 9000000 INFO @ Tue, 16 Jun 2020 11:57:37: 15000000 INFO @ Tue, 16 Jun 2020 11:57:38: 13000000 INFO @ Tue, 16 Jun 2020 11:57:40: 10000000 INFO @ Tue, 16 Jun 2020 11:57:48: 16000000 INFO @ Tue, 16 Jun 2020 11:57:48: 14000000 INFO @ Tue, 16 Jun 2020 11:57:50: 11000000 INFO @ Tue, 16 Jun 2020 11:57:58: 15000000 INFO @ Tue, 16 Jun 2020 11:57:59: 17000000 INFO @ Tue, 16 Jun 2020 11:58:00: 12000000 INFO @ Tue, 16 Jun 2020 11:58:08: 16000000 INFO @ Tue, 16 Jun 2020 11:58:10: 13000000 INFO @ Tue, 16 Jun 2020 11:58:10: 18000000 INFO @ Tue, 16 Jun 2020 11:58:18: 17000000 INFO @ Tue, 16 Jun 2020 11:58:19: 14000000 INFO @ Tue, 16 Jun 2020 11:58:21: 19000000 INFO @ Tue, 16 Jun 2020 11:58:28: 18000000 INFO @ Tue, 16 Jun 2020 11:58:29: 15000000 INFO @ Tue, 16 Jun 2020 11:58:32: 20000000 INFO @ Tue, 16 Jun 2020 11:58:38: 19000000 INFO @ Tue, 16 Jun 2020 11:58:39: 16000000 INFO @ Tue, 16 Jun 2020 11:58:43: 21000000 INFO @ Tue, 16 Jun 2020 11:58:48: 20000000 INFO @ Tue, 16 Jun 2020 11:58:50: 17000000 INFO @ Tue, 16 Jun 2020 11:58:54: 22000000 INFO @ Tue, 16 Jun 2020 11:58:58: 21000000 INFO @ Tue, 16 Jun 2020 11:59:00: 18000000 INFO @ Tue, 16 Jun 2020 11:59:04: 23000000 INFO @ Tue, 16 Jun 2020 11:59:08: 22000000 INFO @ Tue, 16 Jun 2020 11:59:09: 19000000 INFO @ Tue, 16 Jun 2020 11:59:14: 24000000 INFO @ Tue, 16 Jun 2020 11:59:18: 23000000 INFO @ Tue, 16 Jun 2020 11:59:19: 20000000 INFO @ Tue, 16 Jun 2020 11:59:23: 25000000 INFO @ Tue, 16 Jun 2020 11:59:28: 24000000 INFO @ Tue, 16 Jun 2020 11:59:29: 21000000 INFO @ Tue, 16 Jun 2020 11:59:33: 26000000 INFO @ Tue, 16 Jun 2020 11:59:37: 25000000 INFO @ Tue, 16 Jun 2020 11:59:38: 22000000 INFO @ Tue, 16 Jun 2020 11:59:42: 27000000 INFO @ Tue, 16 Jun 2020 11:59:47: 26000000 INFO @ Tue, 16 Jun 2020 11:59:48: 23000000 INFO @ Tue, 16 Jun 2020 11:59:51: 28000000 INFO @ Tue, 16 Jun 2020 11:59:57: 27000000 INFO @ Tue, 16 Jun 2020 11:59:58: 24000000 INFO @ Tue, 16 Jun 2020 12:00:01: 29000000 INFO @ Tue, 16 Jun 2020 12:00:07: 28000000 INFO @ Tue, 16 Jun 2020 12:00:08: 25000000 INFO @ Tue, 16 Jun 2020 12:00:10: 30000000 INFO @ Tue, 16 Jun 2020 12:00:16: 29000000 INFO @ Tue, 16 Jun 2020 12:00:17: 26000000 INFO @ Tue, 16 Jun 2020 12:00:19: 31000000 INFO @ Tue, 16 Jun 2020 12:00:26: 30000000 INFO @ Tue, 16 Jun 2020 12:00:27: 27000000 INFO @ Tue, 16 Jun 2020 12:00:29: 32000000 INFO @ Tue, 16 Jun 2020 12:00:36: 31000000 INFO @ Tue, 16 Jun 2020 12:00:37: 28000000 INFO @ Tue, 16 Jun 2020 12:00:38: 33000000 INFO @ Tue, 16 Jun 2020 12:00:45: 32000000 INFO @ Tue, 16 Jun 2020 12:00:46: 29000000 INFO @ Tue, 16 Jun 2020 12:00:48: 34000000 INFO @ Tue, 16 Jun 2020 12:00:55: 33000000 INFO @ Tue, 16 Jun 2020 12:00:56: 30000000 INFO @ Tue, 16 Jun 2020 12:00:57: 35000000 INFO @ Tue, 16 Jun 2020 12:01:04: 34000000 INFO @ Tue, 16 Jun 2020 12:01:05: 31000000 INFO @ Tue, 16 Jun 2020 12:01:06: 36000000 INFO @ Tue, 16 Jun 2020 12:01:14: 35000000 INFO @ Tue, 16 Jun 2020 12:01:15: 32000000 INFO @ Tue, 16 Jun 2020 12:01:15: 37000000 INFO @ Tue, 16 Jun 2020 12:01:23: 36000000 INFO @ Tue, 16 Jun 2020 12:01:24: 33000000 INFO @ Tue, 16 Jun 2020 12:01:24: 38000000 INFO @ Tue, 16 Jun 2020 12:01:32: 37000000 INFO @ Tue, 16 Jun 2020 12:01:33: 34000000 INFO @ Tue, 16 Jun 2020 12:01:33: 39000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 12:01:41: 38000000 INFO @ Tue, 16 Jun 2020 12:01:42: 40000000 INFO @ Tue, 16 Jun 2020 12:01:42: 35000000 INFO @ Tue, 16 Jun 2020 12:01:51: 39000000 INFO @ Tue, 16 Jun 2020 12:01:52: 36000000 INFO @ Tue, 16 Jun 2020 12:01:52: 41000000 INFO @ Tue, 16 Jun 2020 12:02:00: 40000000 INFO @ Tue, 16 Jun 2020 12:02:01: 37000000 INFO @ Tue, 16 Jun 2020 12:02:01: 42000000 INFO @ Tue, 16 Jun 2020 12:02:09: 41000000 INFO @ Tue, 16 Jun 2020 12:02:10: 38000000 INFO @ Tue, 16 Jun 2020 12:02:10: 43000000 INFO @ Tue, 16 Jun 2020 12:02:18: 42000000 INFO @ Tue, 16 Jun 2020 12:02:19: 44000000 INFO @ Tue, 16 Jun 2020 12:02:19: 39000000 INFO @ Tue, 16 Jun 2020 12:02:28: 43000000 INFO @ Tue, 16 Jun 2020 12:02:28: 45000000 INFO @ Tue, 16 Jun 2020 12:02:28: 40000000 INFO @ Tue, 16 Jun 2020 12:02:37: 44000000 INFO @ Tue, 16 Jun 2020 12:02:37: 46000000 INFO @ Tue, 16 Jun 2020 12:02:38: 41000000 INFO @ Tue, 16 Jun 2020 12:02:46: 47000000 INFO @ Tue, 16 Jun 2020 12:02:47: 45000000 INFO @ Tue, 16 Jun 2020 12:02:47: 42000000 INFO @ Tue, 16 Jun 2020 12:02:55: 48000000 INFO @ Tue, 16 Jun 2020 12:02:56: 46000000 INFO @ Tue, 16 Jun 2020 12:02:56: 43000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 12:03:04: 49000000 INFO @ Tue, 16 Jun 2020 12:03:05: 47000000 INFO @ Tue, 16 Jun 2020 12:03:06: 44000000 INFO @ Tue, 16 Jun 2020 12:03:13: 50000000 INFO @ Tue, 16 Jun 2020 12:03:15: 48000000 INFO @ Tue, 16 Jun 2020 12:03:15: 45000000 INFO @ Tue, 16 Jun 2020 12:03:22: 51000000 INFO @ Tue, 16 Jun 2020 12:03:24: 49000000 INFO @ Tue, 16 Jun 2020 12:03:25: 46000000 INFO @ Tue, 16 Jun 2020 12:03:32: 52000000 INFO @ Tue, 16 Jun 2020 12:03:34: 50000000 INFO @ Tue, 16 Jun 2020 12:03:34: 47000000 INFO @ Tue, 16 Jun 2020 12:03:41: 53000000 INFO @ Tue, 16 Jun 2020 12:03:43: 51000000 INFO @ Tue, 16 Jun 2020 12:03:43: 48000000 INFO @ Tue, 16 Jun 2020 12:03:45: #1 tag size is determined as 150 bps INFO @ Tue, 16 Jun 2020 12:03:45: #1 tag size = 150 INFO @ Tue, 16 Jun 2020 12:03:45: #1 total tags in treatment: 13579669 INFO @ Tue, 16 Jun 2020 12:03:45: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 12:03:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 12:03:45: #1 tags after filtering in treatment: 11370140 INFO @ Tue, 16 Jun 2020 12:03:45: #1 Redundant rate of treatment: 0.16 INFO @ Tue, 16 Jun 2020 12:03:45: #1 finished! INFO @ Tue, 16 Jun 2020 12:03:45: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 12:03:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 12:03:46: #2 number of paired peaks: 278 WARNING @ Tue, 16 Jun 2020 12:03:46: Fewer paired peaks (278) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 278 pairs to build model! INFO @ Tue, 16 Jun 2020 12:03:46: start model_add_line... INFO @ Tue, 16 Jun 2020 12:03:46: start X-correlation... INFO @ Tue, 16 Jun 2020 12:03:46: end of X-cor INFO @ Tue, 16 Jun 2020 12:03:46: #2 finished! INFO @ Tue, 16 Jun 2020 12:03:46: #2 predicted fragment length is 213 bps INFO @ Tue, 16 Jun 2020 12:03:46: #2 alternative fragment length(s) may be 213 bps INFO @ Tue, 16 Jun 2020 12:03:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX6619582/SRX6619582.05_model.r WARNING @ Tue, 16 Jun 2020 12:03:46: #2 Since the d (213) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 12:03:46: #2 You may need to consider one of the other alternative d(s): 213 WARNING @ Tue, 16 Jun 2020 12:03:46: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 12:03:46: #3 Call peaks... INFO @ Tue, 16 Jun 2020 12:03:46: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 12:03:52: 52000000 INFO @ Tue, 16 Jun 2020 12:03:52: 49000000 INFO @ Tue, 16 Jun 2020 12:04:01: 53000000 INFO @ Tue, 16 Jun 2020 12:04:01: 50000000 INFO @ Tue, 16 Jun 2020 12:04:05: #1 tag size is determined as 150 bps INFO @ Tue, 16 Jun 2020 12:04:05: #1 tag size = 150 INFO @ Tue, 16 Jun 2020 12:04:05: #1 total tags in treatment: 13579669 INFO @ Tue, 16 Jun 2020 12:04:05: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 12:04:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 12:04:05: #1 tags after filtering in treatment: 11370140 INFO @ Tue, 16 Jun 2020 12:04:05: #1 Redundant rate of treatment: 0.16 INFO @ Tue, 16 Jun 2020 12:04:05: #1 finished! INFO @ Tue, 16 Jun 2020 12:04:05: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 12:04:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 12:04:06: #2 number of paired peaks: 278 WARNING @ Tue, 16 Jun 2020 12:04:06: Fewer paired peaks (278) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 278 pairs to build model! INFO @ Tue, 16 Jun 2020 12:04:06: start model_add_line... INFO @ Tue, 16 Jun 2020 12:04:06: start X-correlation... INFO @ Tue, 16 Jun 2020 12:04:06: end of X-cor INFO @ Tue, 16 Jun 2020 12:04:06: #2 finished! INFO @ Tue, 16 Jun 2020 12:04:06: #2 predicted fragment length is 213 bps INFO @ Tue, 16 Jun 2020 12:04:06: #2 alternative fragment length(s) may be 213 bps INFO @ Tue, 16 Jun 2020 12:04:06: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX6619582/SRX6619582.10_model.r WARNING @ Tue, 16 Jun 2020 12:04:06: #2 Since the d (213) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 12:04:06: #2 You may need to consider one of the other alternative d(s): 213 WARNING @ Tue, 16 Jun 2020 12:04:06: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 12:04:06: #3 Call peaks... INFO @ Tue, 16 Jun 2020 12:04:06: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 12:04:10: 51000000 INFO @ Tue, 16 Jun 2020 12:04:12: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 12:04:18: 52000000 INFO @ Tue, 16 Jun 2020 12:04:24: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX6619582/SRX6619582.05_peaks.xls INFO @ Tue, 16 Jun 2020 12:04:24: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX6619582/SRX6619582.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 12:04:24: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX6619582/SRX6619582.05_summits.bed INFO @ Tue, 16 Jun 2020 12:04:24: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (524 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 12:04:26: 53000000 INFO @ Tue, 16 Jun 2020 12:04:29: #1 tag size is determined as 150 bps INFO @ Tue, 16 Jun 2020 12:04:29: #1 tag size = 150 INFO @ Tue, 16 Jun 2020 12:04:29: #1 total tags in treatment: 13579669 INFO @ Tue, 16 Jun 2020 12:04:29: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 12:04:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 12:04:30: #1 tags after filtering in treatment: 11370140 INFO @ Tue, 16 Jun 2020 12:04:30: #1 Redundant rate of treatment: 0.16 INFO @ Tue, 16 Jun 2020 12:04:30: #1 finished! INFO @ Tue, 16 Jun 2020 12:04:30: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 12:04:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 12:04:30: #2 number of paired peaks: 278 WARNING @ Tue, 16 Jun 2020 12:04:30: Fewer paired peaks (278) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 278 pairs to build model! INFO @ Tue, 16 Jun 2020 12:04:30: start model_add_line... INFO @ Tue, 16 Jun 2020 12:04:31: start X-correlation... INFO @ Tue, 16 Jun 2020 12:04:31: end of X-cor INFO @ Tue, 16 Jun 2020 12:04:31: #2 finished! INFO @ Tue, 16 Jun 2020 12:04:31: #2 predicted fragment length is 213 bps INFO @ Tue, 16 Jun 2020 12:04:31: #2 alternative fragment length(s) may be 213 bps INFO @ Tue, 16 Jun 2020 12:04:31: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX6619582/SRX6619582.20_model.r WARNING @ Tue, 16 Jun 2020 12:04:31: #2 Since the d (213) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 12:04:31: #2 You may need to consider one of the other alternative d(s): 213 WARNING @ Tue, 16 Jun 2020 12:04:31: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 12:04:31: #3 Call peaks... INFO @ Tue, 16 Jun 2020 12:04:31: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 12:04:32: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 12:04:44: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX6619582/SRX6619582.10_peaks.xls INFO @ Tue, 16 Jun 2020 12:04:44: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX6619582/SRX6619582.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 12:04:44: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX6619582/SRX6619582.10_summits.bed INFO @ Tue, 16 Jun 2020 12:04:44: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (356 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 12:04:56: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 12:05:08: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX6619582/SRX6619582.20_peaks.xls INFO @ Tue, 16 Jun 2020 12:05:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX6619582/SRX6619582.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 12:05:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX6619582/SRX6619582.20_summits.bed INFO @ Tue, 16 Jun 2020 12:05:08: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (221 records, 4 fields): 1 millis CompletedMACS2peakCalling