Job ID = 6368876 SRX = SRX6619573 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-06-16T00:36:46 prefetch.2.10.7: 1) Downloading 'SRR9866036'... 2020-06-16T00:36:46 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:56:56 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:56:56 prefetch.2.10.7: 1) 'SRR9866036' was downloaded successfully 2020-06-16T00:56:56 prefetch.2.10.7: 'SRR9866036' has 0 unresolved dependencies Read 43788202 spots for SRR9866036/SRR9866036.sra Written 43788202 spots for SRR9866036/SRR9866036.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:35:42 43788202 reads; of these: 43788202 (100.00%) were paired; of these: 23576760 (53.84%) aligned concordantly 0 times 15132741 (34.56%) aligned concordantly exactly 1 time 5078701 (11.60%) aligned concordantly >1 times ---- 23576760 pairs aligned concordantly 0 times; of these: 10852539 (46.03%) aligned discordantly 1 time ---- 12724221 pairs aligned 0 times concordantly or discordantly; of these: 25448442 mates make up the pairs; of these: 21075973 (82.82%) aligned 0 times 1228351 (4.83%) aligned exactly 1 time 3144118 (12.35%) aligned >1 times 75.93% overall alignment rate Time searching: 01:35:42 Overall time: 01:35:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 52 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 5335732 / 30543664 = 0.1747 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 12:08:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619573/SRX6619573.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619573/SRX6619573.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619573/SRX6619573.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619573/SRX6619573.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 12:08:40: #1 read tag files... INFO @ Tue, 16 Jun 2020 12:08:40: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 12:08:48: 1000000 INFO @ Tue, 16 Jun 2020 12:08:56: 2000000 INFO @ Tue, 16 Jun 2020 12:09:05: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 12:09:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619573/SRX6619573.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619573/SRX6619573.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619573/SRX6619573.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619573/SRX6619573.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 12:09:10: #1 read tag files... INFO @ Tue, 16 Jun 2020 12:09:10: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 12:09:13: 4000000 INFO @ Tue, 16 Jun 2020 12:09:20: 1000000 INFO @ Tue, 16 Jun 2020 12:09:22: 5000000 INFO @ Tue, 16 Jun 2020 12:09:29: 2000000 INFO @ Tue, 16 Jun 2020 12:09:31: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 12:09:39: 3000000 INFO @ Tue, 16 Jun 2020 12:09:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619573/SRX6619573.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619573/SRX6619573.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619573/SRX6619573.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619573/SRX6619573.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 12:09:40: #1 read tag files... INFO @ Tue, 16 Jun 2020 12:09:40: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 12:09:41: 7000000 INFO @ Tue, 16 Jun 2020 12:09:49: 4000000 INFO @ Tue, 16 Jun 2020 12:09:50: 8000000 INFO @ Tue, 16 Jun 2020 12:09:50: 1000000 INFO @ Tue, 16 Jun 2020 12:09:59: 5000000 INFO @ Tue, 16 Jun 2020 12:10:00: 9000000 INFO @ Tue, 16 Jun 2020 12:10:00: 2000000 INFO @ Tue, 16 Jun 2020 12:10:09: 6000000 INFO @ Tue, 16 Jun 2020 12:10:09: 10000000 INFO @ Tue, 16 Jun 2020 12:10:10: 3000000 INFO @ Tue, 16 Jun 2020 12:10:18: 7000000 INFO @ Tue, 16 Jun 2020 12:10:19: 11000000 INFO @ Tue, 16 Jun 2020 12:10:20: 4000000 INFO @ Tue, 16 Jun 2020 12:10:28: 12000000 INFO @ Tue, 16 Jun 2020 12:10:29: 8000000 INFO @ Tue, 16 Jun 2020 12:10:30: 5000000 INFO @ Tue, 16 Jun 2020 12:10:38: 13000000 INFO @ Tue, 16 Jun 2020 12:10:38: 9000000 INFO @ Tue, 16 Jun 2020 12:10:40: 6000000 INFO @ Tue, 16 Jun 2020 12:10:48: 14000000 INFO @ Tue, 16 Jun 2020 12:10:48: 10000000 INFO @ Tue, 16 Jun 2020 12:10:50: 7000000 INFO @ Tue, 16 Jun 2020 12:10:57: 15000000 INFO @ Tue, 16 Jun 2020 12:10:57: 11000000 INFO @ Tue, 16 Jun 2020 12:10:59: 8000000 INFO @ Tue, 16 Jun 2020 12:11:07: 16000000 INFO @ Tue, 16 Jun 2020 12:11:07: 12000000 INFO @ Tue, 16 Jun 2020 12:11:09: 9000000 INFO @ Tue, 16 Jun 2020 12:11:16: 13000000 INFO @ Tue, 16 Jun 2020 12:11:16: 17000000 INFO @ Tue, 16 Jun 2020 12:11:18: 10000000 INFO @ Tue, 16 Jun 2020 12:11:26: 14000000 INFO @ Tue, 16 Jun 2020 12:11:26: 18000000 INFO @ Tue, 16 Jun 2020 12:11:28: 11000000 INFO @ Tue, 16 Jun 2020 12:11:36: 15000000 INFO @ Tue, 16 Jun 2020 12:11:36: 19000000 INFO @ Tue, 16 Jun 2020 12:11:38: 12000000 INFO @ Tue, 16 Jun 2020 12:11:45: 16000000 INFO @ Tue, 16 Jun 2020 12:11:45: 20000000 INFO @ Tue, 16 Jun 2020 12:11:47: 13000000 INFO @ Tue, 16 Jun 2020 12:11:55: 17000000 INFO @ Tue, 16 Jun 2020 12:11:55: 21000000 INFO @ Tue, 16 Jun 2020 12:11:57: 14000000 INFO @ Tue, 16 Jun 2020 12:12:04: 18000000 INFO @ Tue, 16 Jun 2020 12:12:04: 22000000 INFO @ Tue, 16 Jun 2020 12:12:07: 15000000 INFO @ Tue, 16 Jun 2020 12:12:14: 19000000 INFO @ Tue, 16 Jun 2020 12:12:14: 23000000 INFO @ Tue, 16 Jun 2020 12:12:17: 16000000 INFO @ Tue, 16 Jun 2020 12:12:23: 20000000 INFO @ Tue, 16 Jun 2020 12:12:24: 24000000 INFO @ Tue, 16 Jun 2020 12:12:27: 17000000 INFO @ Tue, 16 Jun 2020 12:12:33: 25000000 INFO @ Tue, 16 Jun 2020 12:12:34: 21000000 INFO @ Tue, 16 Jun 2020 12:12:37: 18000000 INFO @ Tue, 16 Jun 2020 12:12:43: 26000000 INFO @ Tue, 16 Jun 2020 12:12:43: 22000000 INFO @ Tue, 16 Jun 2020 12:12:47: 19000000 INFO @ Tue, 16 Jun 2020 12:12:53: 27000000 INFO @ Tue, 16 Jun 2020 12:12:54: 23000000 INFO @ Tue, 16 Jun 2020 12:12:57: 20000000 INFO @ Tue, 16 Jun 2020 12:13:03: 28000000 INFO @ Tue, 16 Jun 2020 12:13:03: 24000000 INFO @ Tue, 16 Jun 2020 12:13:06: 21000000 INFO @ Tue, 16 Jun 2020 12:13:13: 25000000 INFO @ Tue, 16 Jun 2020 12:13:13: 29000000 INFO @ Tue, 16 Jun 2020 12:13:16: 22000000 INFO @ Tue, 16 Jun 2020 12:13:23: 26000000 INFO @ Tue, 16 Jun 2020 12:13:23: 30000000 INFO @ Tue, 16 Jun 2020 12:13:27: 23000000 INFO @ Tue, 16 Jun 2020 12:13:33: 27000000 INFO @ Tue, 16 Jun 2020 12:13:33: 31000000 INFO @ Tue, 16 Jun 2020 12:13:37: 24000000 INFO @ Tue, 16 Jun 2020 12:13:43: 28000000 INFO @ Tue, 16 Jun 2020 12:13:43: 32000000 INFO @ Tue, 16 Jun 2020 12:13:47: 25000000 INFO @ Tue, 16 Jun 2020 12:13:53: 29000000 INFO @ Tue, 16 Jun 2020 12:13:54: 33000000 INFO @ Tue, 16 Jun 2020 12:13:58: 26000000 INFO @ Tue, 16 Jun 2020 12:14:03: 30000000 INFO @ Tue, 16 Jun 2020 12:14:04: 34000000 INFO @ Tue, 16 Jun 2020 12:14:08: 27000000 INFO @ Tue, 16 Jun 2020 12:14:13: 31000000 INFO @ Tue, 16 Jun 2020 12:14:14: 35000000 INFO @ Tue, 16 Jun 2020 12:14:18: 28000000 INFO @ Tue, 16 Jun 2020 12:14:23: 32000000 INFO @ Tue, 16 Jun 2020 12:14:24: 36000000 INFO @ Tue, 16 Jun 2020 12:14:28: 29000000 INFO @ Tue, 16 Jun 2020 12:14:33: 33000000 INFO @ Tue, 16 Jun 2020 12:14:34: 37000000 INFO @ Tue, 16 Jun 2020 12:14:38: 30000000 INFO @ Tue, 16 Jun 2020 12:14:43: 34000000 INFO @ Tue, 16 Jun 2020 12:14:43: 38000000 INFO @ Tue, 16 Jun 2020 12:14:48: 31000000 INFO @ Tue, 16 Jun 2020 12:14:53: 35000000 INFO @ Tue, 16 Jun 2020 12:14:53: 39000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 12:14:58: 32000000 INFO @ Tue, 16 Jun 2020 12:15:03: 36000000 INFO @ Tue, 16 Jun 2020 12:15:03: 40000000 INFO @ Tue, 16 Jun 2020 12:15:09: 33000000 INFO @ Tue, 16 Jun 2020 12:15:13: 37000000 INFO @ Tue, 16 Jun 2020 12:15:14: 41000000 INFO @ Tue, 16 Jun 2020 12:15:18: 34000000 INFO @ Tue, 16 Jun 2020 12:15:23: 38000000 INFO @ Tue, 16 Jun 2020 12:15:23: 42000000 INFO @ Tue, 16 Jun 2020 12:15:28: 35000000 INFO @ Tue, 16 Jun 2020 12:15:32: 39000000 INFO @ Tue, 16 Jun 2020 12:15:33: 43000000 INFO @ Tue, 16 Jun 2020 12:15:38: 36000000 INFO @ Tue, 16 Jun 2020 12:15:42: 40000000 INFO @ Tue, 16 Jun 2020 12:15:43: 44000000 INFO @ Tue, 16 Jun 2020 12:15:48: 37000000 INFO @ Tue, 16 Jun 2020 12:15:51: 41000000 INFO @ Tue, 16 Jun 2020 12:15:52: 45000000 INFO @ Tue, 16 Jun 2020 12:15:58: 38000000 INFO @ Tue, 16 Jun 2020 12:16:01: 42000000 INFO @ Tue, 16 Jun 2020 12:16:02: 46000000 INFO @ Tue, 16 Jun 2020 12:16:07: 39000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 12:16:11: 43000000 INFO @ Tue, 16 Jun 2020 12:16:12: 47000000 INFO @ Tue, 16 Jun 2020 12:16:17: 40000000 INFO @ Tue, 16 Jun 2020 12:16:20: 44000000 INFO @ Tue, 16 Jun 2020 12:16:22: 48000000 INFO @ Tue, 16 Jun 2020 12:16:27: 41000000 INFO @ Tue, 16 Jun 2020 12:16:30: 45000000 INFO @ Tue, 16 Jun 2020 12:16:31: 49000000 INFO @ Tue, 16 Jun 2020 12:16:36: 42000000 INFO @ Tue, 16 Jun 2020 12:16:40: 46000000 INFO @ Tue, 16 Jun 2020 12:16:41: 50000000 INFO @ Tue, 16 Jun 2020 12:16:46: 43000000 INFO @ Tue, 16 Jun 2020 12:16:50: 47000000 INFO @ Tue, 16 Jun 2020 12:16:51: 51000000 INFO @ Tue, 16 Jun 2020 12:16:56: 44000000 INFO @ Tue, 16 Jun 2020 12:17:00: 48000000 INFO @ Tue, 16 Jun 2020 12:17:01: 52000000 INFO @ Tue, 16 Jun 2020 12:17:05: 45000000 INFO @ Tue, 16 Jun 2020 12:17:09: 49000000 INFO @ Tue, 16 Jun 2020 12:17:11: 53000000 INFO @ Tue, 16 Jun 2020 12:17:15: 46000000 INFO @ Tue, 16 Jun 2020 12:17:19: 50000000 INFO @ Tue, 16 Jun 2020 12:17:21: 54000000 INFO @ Tue, 16 Jun 2020 12:17:25: 47000000 INFO @ Tue, 16 Jun 2020 12:17:29: 51000000 INFO @ Tue, 16 Jun 2020 12:17:31: 55000000 INFO @ Tue, 16 Jun 2020 12:17:34: 48000000 INFO @ Tue, 16 Jun 2020 12:17:39: 52000000 INFO @ Tue, 16 Jun 2020 12:17:39: #1 tag size is determined as 150 bps INFO @ Tue, 16 Jun 2020 12:17:39: #1 tag size = 150 INFO @ Tue, 16 Jun 2020 12:17:39: #1 total tags in treatment: 16357437 INFO @ Tue, 16 Jun 2020 12:17:39: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 12:17:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 12:17:40: #1 tags after filtering in treatment: 12481812 INFO @ Tue, 16 Jun 2020 12:17:40: #1 Redundant rate of treatment: 0.24 INFO @ Tue, 16 Jun 2020 12:17:40: #1 finished! INFO @ Tue, 16 Jun 2020 12:17:40: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 12:17:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 12:17:40: #2 number of paired peaks: 333 WARNING @ Tue, 16 Jun 2020 12:17:40: Fewer paired peaks (333) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 333 pairs to build model! INFO @ Tue, 16 Jun 2020 12:17:40: start model_add_line... INFO @ Tue, 16 Jun 2020 12:17:41: start X-correlation... INFO @ Tue, 16 Jun 2020 12:17:41: end of X-cor INFO @ Tue, 16 Jun 2020 12:17:41: #2 finished! INFO @ Tue, 16 Jun 2020 12:17:41: #2 predicted fragment length is 200 bps INFO @ Tue, 16 Jun 2020 12:17:41: #2 alternative fragment length(s) may be 200 bps INFO @ Tue, 16 Jun 2020 12:17:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX6619573/SRX6619573.05_model.r WARNING @ Tue, 16 Jun 2020 12:17:41: #2 Since the d (200) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 12:17:41: #2 You may need to consider one of the other alternative d(s): 200 WARNING @ Tue, 16 Jun 2020 12:17:41: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 12:17:41: #3 Call peaks... INFO @ Tue, 16 Jun 2020 12:17:41: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 12:17:44: 49000000 INFO @ Tue, 16 Jun 2020 12:17:49: 53000000 INFO @ Tue, 16 Jun 2020 12:17:54: 50000000 INFO @ Tue, 16 Jun 2020 12:17:59: 54000000 INFO @ Tue, 16 Jun 2020 12:18:04: 51000000 INFO @ Tue, 16 Jun 2020 12:18:08: 55000000 INFO @ Tue, 16 Jun 2020 12:18:11: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 12:18:13: 52000000 INFO @ Tue, 16 Jun 2020 12:18:17: #1 tag size is determined as 150 bps INFO @ Tue, 16 Jun 2020 12:18:17: #1 tag size = 150 INFO @ Tue, 16 Jun 2020 12:18:17: #1 total tags in treatment: 16357437 INFO @ Tue, 16 Jun 2020 12:18:17: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 12:18:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 12:18:17: #1 tags after filtering in treatment: 12481812 INFO @ Tue, 16 Jun 2020 12:18:17: #1 Redundant rate of treatment: 0.24 INFO @ Tue, 16 Jun 2020 12:18:17: #1 finished! INFO @ Tue, 16 Jun 2020 12:18:17: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 12:18:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 12:18:18: #2 number of paired peaks: 333 WARNING @ Tue, 16 Jun 2020 12:18:18: Fewer paired peaks (333) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 333 pairs to build model! INFO @ Tue, 16 Jun 2020 12:18:18: start model_add_line... INFO @ Tue, 16 Jun 2020 12:18:18: start X-correlation... INFO @ Tue, 16 Jun 2020 12:18:18: end of X-cor INFO @ Tue, 16 Jun 2020 12:18:18: #2 finished! INFO @ Tue, 16 Jun 2020 12:18:18: #2 predicted fragment length is 200 bps INFO @ Tue, 16 Jun 2020 12:18:18: #2 alternative fragment length(s) may be 200 bps INFO @ Tue, 16 Jun 2020 12:18:18: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX6619573/SRX6619573.10_model.r WARNING @ Tue, 16 Jun 2020 12:18:18: #2 Since the d (200) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 12:18:18: #2 You may need to consider one of the other alternative d(s): 200 WARNING @ Tue, 16 Jun 2020 12:18:18: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 12:18:18: #3 Call peaks... INFO @ Tue, 16 Jun 2020 12:18:18: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 12:18:23: 53000000 INFO @ Tue, 16 Jun 2020 12:18:26: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX6619573/SRX6619573.05_peaks.xls INFO @ Tue, 16 Jun 2020 12:18:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX6619573/SRX6619573.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 12:18:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX6619573/SRX6619573.05_summits.bed INFO @ Tue, 16 Jun 2020 12:18:26: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (847 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 12:18:31: 54000000 INFO @ Tue, 16 Jun 2020 12:18:40: 55000000 INFO @ Tue, 16 Jun 2020 12:18:47: #1 tag size is determined as 150 bps INFO @ Tue, 16 Jun 2020 12:18:47: #1 tag size = 150 INFO @ Tue, 16 Jun 2020 12:18:47: #1 total tags in treatment: 16357437 INFO @ Tue, 16 Jun 2020 12:18:47: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 12:18:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 12:18:47: #1 tags after filtering in treatment: 12481812 INFO @ Tue, 16 Jun 2020 12:18:47: #1 Redundant rate of treatment: 0.24 INFO @ Tue, 16 Jun 2020 12:18:47: #1 finished! INFO @ Tue, 16 Jun 2020 12:18:47: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 12:18:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 12:18:48: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 12:18:48: #2 number of paired peaks: 333 WARNING @ Tue, 16 Jun 2020 12:18:48: Fewer paired peaks (333) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 333 pairs to build model! INFO @ Tue, 16 Jun 2020 12:18:48: start model_add_line... INFO @ Tue, 16 Jun 2020 12:18:48: start X-correlation... INFO @ Tue, 16 Jun 2020 12:18:48: end of X-cor INFO @ Tue, 16 Jun 2020 12:18:48: #2 finished! INFO @ Tue, 16 Jun 2020 12:18:48: #2 predicted fragment length is 200 bps INFO @ Tue, 16 Jun 2020 12:18:48: #2 alternative fragment length(s) may be 200 bps INFO @ Tue, 16 Jun 2020 12:18:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX6619573/SRX6619573.20_model.r WARNING @ Tue, 16 Jun 2020 12:18:48: #2 Since the d (200) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 12:18:48: #2 You may need to consider one of the other alternative d(s): 200 WARNING @ Tue, 16 Jun 2020 12:18:48: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 12:18:48: #3 Call peaks... INFO @ Tue, 16 Jun 2020 12:18:48: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 12:19:03: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX6619573/SRX6619573.10_peaks.xls INFO @ Tue, 16 Jun 2020 12:19:03: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX6619573/SRX6619573.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 12:19:03: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX6619573/SRX6619573.10_summits.bed INFO @ Tue, 16 Jun 2020 12:19:03: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (406 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 12:19:18: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 12:19:33: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX6619573/SRX6619573.20_peaks.xls INFO @ Tue, 16 Jun 2020 12:19:33: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX6619573/SRX6619573.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 12:19:33: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX6619573/SRX6619573.20_summits.bed INFO @ Tue, 16 Jun 2020 12:19:33: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (216 records, 4 fields): 1 millis CompletedMACS2peakCalling