Job ID = 6368874 SRX = SRX6619571 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-06-16T00:27:19 prefetch.2.10.7: 1) Downloading 'SRR9866038'... 2020-06-16T00:27:19 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:46:01 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:46:01 prefetch.2.10.7: 1) 'SRR9866038' was downloaded successfully 2020-06-16T00:46:01 prefetch.2.10.7: 'SRR9866038' has 0 unresolved dependencies Read 33979047 spots for SRR9866038/SRR9866038.sra Written 33979047 spots for SRR9866038/SRR9866038.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:20:38 33979047 reads; of these: 33979047 (100.00%) were paired; of these: 16235637 (47.78%) aligned concordantly 0 times 13149845 (38.70%) aligned concordantly exactly 1 time 4593565 (13.52%) aligned concordantly >1 times ---- 16235637 pairs aligned concordantly 0 times; of these: 7146312 (44.02%) aligned discordantly 1 time ---- 9089325 pairs aligned 0 times concordantly or discordantly; of these: 18178650 mates make up the pairs; of these: 14837755 (81.62%) aligned 0 times 1171043 (6.44%) aligned exactly 1 time 2169852 (11.94%) aligned >1 times 78.17% overall alignment rate Time searching: 01:20:38 Overall time: 01:20:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 3806017 / 24535149 = 0.1551 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 11:40:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619571/SRX6619571.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619571/SRX6619571.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619571/SRX6619571.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619571/SRX6619571.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 11:40:18: #1 read tag files... INFO @ Tue, 16 Jun 2020 11:40:18: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 11:40:25: 1000000 INFO @ Tue, 16 Jun 2020 11:40:33: 2000000 INFO @ Tue, 16 Jun 2020 11:40:40: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 11:40:48: 4000000 INFO @ Tue, 16 Jun 2020 11:40:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619571/SRX6619571.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619571/SRX6619571.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619571/SRX6619571.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619571/SRX6619571.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 11:40:48: #1 read tag files... INFO @ Tue, 16 Jun 2020 11:40:48: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 11:40:56: 5000000 INFO @ Tue, 16 Jun 2020 11:40:56: 1000000 INFO @ Tue, 16 Jun 2020 11:41:03: 6000000 INFO @ Tue, 16 Jun 2020 11:41:04: 2000000 INFO @ Tue, 16 Jun 2020 11:41:11: 7000000 INFO @ Tue, 16 Jun 2020 11:41:12: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 11:41:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619571/SRX6619571.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619571/SRX6619571.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619571/SRX6619571.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619571/SRX6619571.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 11:41:18: #1 read tag files... INFO @ Tue, 16 Jun 2020 11:41:18: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 11:41:18: 8000000 INFO @ Tue, 16 Jun 2020 11:41:20: 4000000 INFO @ Tue, 16 Jun 2020 11:41:26: 9000000 INFO @ Tue, 16 Jun 2020 11:41:26: 1000000 INFO @ Tue, 16 Jun 2020 11:41:28: 5000000 INFO @ Tue, 16 Jun 2020 11:41:34: 10000000 INFO @ Tue, 16 Jun 2020 11:41:34: 2000000 INFO @ Tue, 16 Jun 2020 11:41:36: 6000000 INFO @ Tue, 16 Jun 2020 11:41:41: 11000000 INFO @ Tue, 16 Jun 2020 11:41:42: 3000000 INFO @ Tue, 16 Jun 2020 11:41:44: 7000000 INFO @ Tue, 16 Jun 2020 11:41:49: 12000000 INFO @ Tue, 16 Jun 2020 11:41:51: 4000000 INFO @ Tue, 16 Jun 2020 11:41:51: 8000000 INFO @ Tue, 16 Jun 2020 11:41:57: 13000000 INFO @ Tue, 16 Jun 2020 11:41:59: 5000000 INFO @ Tue, 16 Jun 2020 11:41:59: 9000000 INFO @ Tue, 16 Jun 2020 11:42:04: 14000000 INFO @ Tue, 16 Jun 2020 11:42:07: 6000000 INFO @ Tue, 16 Jun 2020 11:42:07: 10000000 INFO @ Tue, 16 Jun 2020 11:42:12: 15000000 INFO @ Tue, 16 Jun 2020 11:42:15: 7000000 INFO @ Tue, 16 Jun 2020 11:42:15: 11000000 INFO @ Tue, 16 Jun 2020 11:42:20: 16000000 INFO @ Tue, 16 Jun 2020 11:42:23: 12000000 INFO @ Tue, 16 Jun 2020 11:42:23: 8000000 INFO @ Tue, 16 Jun 2020 11:42:28: 17000000 INFO @ Tue, 16 Jun 2020 11:42:31: 13000000 INFO @ Tue, 16 Jun 2020 11:42:31: 9000000 INFO @ Tue, 16 Jun 2020 11:42:35: 18000000 INFO @ Tue, 16 Jun 2020 11:42:39: 14000000 INFO @ Tue, 16 Jun 2020 11:42:39: 10000000 INFO @ Tue, 16 Jun 2020 11:42:43: 19000000 INFO @ Tue, 16 Jun 2020 11:42:47: 15000000 INFO @ Tue, 16 Jun 2020 11:42:47: 11000000 INFO @ Tue, 16 Jun 2020 11:42:50: 20000000 INFO @ Tue, 16 Jun 2020 11:42:55: 16000000 INFO @ Tue, 16 Jun 2020 11:42:55: 12000000 INFO @ Tue, 16 Jun 2020 11:42:58: 21000000 INFO @ Tue, 16 Jun 2020 11:43:02: 17000000 INFO @ Tue, 16 Jun 2020 11:43:03: 13000000 INFO @ Tue, 16 Jun 2020 11:43:06: 22000000 INFO @ Tue, 16 Jun 2020 11:43:10: 18000000 INFO @ Tue, 16 Jun 2020 11:43:12: 14000000 INFO @ Tue, 16 Jun 2020 11:43:13: 23000000 INFO @ Tue, 16 Jun 2020 11:43:18: 19000000 INFO @ Tue, 16 Jun 2020 11:43:20: 15000000 INFO @ Tue, 16 Jun 2020 11:43:21: 24000000 INFO @ Tue, 16 Jun 2020 11:43:26: 20000000 INFO @ Tue, 16 Jun 2020 11:43:28: 16000000 INFO @ Tue, 16 Jun 2020 11:43:29: 25000000 INFO @ Tue, 16 Jun 2020 11:43:34: 21000000 INFO @ Tue, 16 Jun 2020 11:43:36: 17000000 INFO @ Tue, 16 Jun 2020 11:43:36: 26000000 INFO @ Tue, 16 Jun 2020 11:43:41: 22000000 INFO @ Tue, 16 Jun 2020 11:43:44: 18000000 INFO @ Tue, 16 Jun 2020 11:43:44: 27000000 INFO @ Tue, 16 Jun 2020 11:43:49: 23000000 INFO @ Tue, 16 Jun 2020 11:43:52: 19000000 INFO @ Tue, 16 Jun 2020 11:43:52: 28000000 INFO @ Tue, 16 Jun 2020 11:43:57: 24000000 INFO @ Tue, 16 Jun 2020 11:44:00: 29000000 INFO @ Tue, 16 Jun 2020 11:44:00: 20000000 INFO @ Tue, 16 Jun 2020 11:44:04: 25000000 INFO @ Tue, 16 Jun 2020 11:44:07: 30000000 INFO @ Tue, 16 Jun 2020 11:44:08: 21000000 INFO @ Tue, 16 Jun 2020 11:44:12: 26000000 INFO @ Tue, 16 Jun 2020 11:44:15: 31000000 INFO @ Tue, 16 Jun 2020 11:44:16: 22000000 INFO @ Tue, 16 Jun 2020 11:44:20: 27000000 INFO @ Tue, 16 Jun 2020 11:44:23: 32000000 INFO @ Tue, 16 Jun 2020 11:44:24: 23000000 INFO @ Tue, 16 Jun 2020 11:44:27: 28000000 INFO @ Tue, 16 Jun 2020 11:44:31: 33000000 INFO @ Tue, 16 Jun 2020 11:44:32: 24000000 INFO @ Tue, 16 Jun 2020 11:44:35: 29000000 INFO @ Tue, 16 Jun 2020 11:44:38: 34000000 INFO @ Tue, 16 Jun 2020 11:44:40: 25000000 INFO @ Tue, 16 Jun 2020 11:44:43: 30000000 INFO @ Tue, 16 Jun 2020 11:44:46: 35000000 INFO @ Tue, 16 Jun 2020 11:44:48: 26000000 INFO @ Tue, 16 Jun 2020 11:44:50: 31000000 INFO @ Tue, 16 Jun 2020 11:44:54: 36000000 INFO @ Tue, 16 Jun 2020 11:44:56: 27000000 INFO @ Tue, 16 Jun 2020 11:44:58: 32000000 INFO @ Tue, 16 Jun 2020 11:45:02: 37000000 INFO @ Tue, 16 Jun 2020 11:45:04: 28000000 INFO @ Tue, 16 Jun 2020 11:45:05: 33000000 INFO @ Tue, 16 Jun 2020 11:45:10: 38000000 INFO @ Tue, 16 Jun 2020 11:45:12: 29000000 INFO @ Tue, 16 Jun 2020 11:45:13: 34000000 INFO @ Tue, 16 Jun 2020 11:45:18: 39000000 INFO @ Tue, 16 Jun 2020 11:45:20: 30000000 INFO @ Tue, 16 Jun 2020 11:45:21: 35000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 11:45:26: 40000000 INFO @ Tue, 16 Jun 2020 11:45:28: 31000000 INFO @ Tue, 16 Jun 2020 11:45:29: 36000000 INFO @ Tue, 16 Jun 2020 11:45:34: 41000000 INFO @ Tue, 16 Jun 2020 11:45:35: 32000000 INFO @ Tue, 16 Jun 2020 11:45:37: 37000000 INFO @ Tue, 16 Jun 2020 11:45:42: 42000000 INFO @ Tue, 16 Jun 2020 11:45:43: 33000000 INFO @ Tue, 16 Jun 2020 11:45:44: 38000000 INFO @ Tue, 16 Jun 2020 11:45:50: 43000000 INFO @ Tue, 16 Jun 2020 11:45:51: 34000000 INFO @ Tue, 16 Jun 2020 11:45:52: 39000000 INFO @ Tue, 16 Jun 2020 11:45:57: 44000000 INFO @ Tue, 16 Jun 2020 11:45:59: 35000000 INFO @ Tue, 16 Jun 2020 11:46:00: 40000000 INFO @ Tue, 16 Jun 2020 11:46:05: 45000000 INFO @ Tue, 16 Jun 2020 11:46:07: 36000000 INFO @ Tue, 16 Jun 2020 11:46:08: 41000000 INFO @ Tue, 16 Jun 2020 11:46:09: #1 tag size is determined as 150 bps INFO @ Tue, 16 Jun 2020 11:46:09: #1 tag size = 150 INFO @ Tue, 16 Jun 2020 11:46:09: #1 total tags in treatment: 14700099 INFO @ Tue, 16 Jun 2020 11:46:09: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 11:46:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 11:46:10: #1 tags after filtering in treatment: 11339203 INFO @ Tue, 16 Jun 2020 11:46:10: #1 Redundant rate of treatment: 0.23 INFO @ Tue, 16 Jun 2020 11:46:10: #1 finished! INFO @ Tue, 16 Jun 2020 11:46:10: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 11:46:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 11:46:10: #2 number of paired peaks: 451 WARNING @ Tue, 16 Jun 2020 11:46:10: Fewer paired peaks (451) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 451 pairs to build model! INFO @ Tue, 16 Jun 2020 11:46:10: start model_add_line... INFO @ Tue, 16 Jun 2020 11:46:11: start X-correlation... INFO @ Tue, 16 Jun 2020 11:46:11: end of X-cor INFO @ Tue, 16 Jun 2020 11:46:11: #2 finished! INFO @ Tue, 16 Jun 2020 11:46:11: #2 predicted fragment length is 211 bps INFO @ Tue, 16 Jun 2020 11:46:11: #2 alternative fragment length(s) may be 211,225 bps INFO @ Tue, 16 Jun 2020 11:46:11: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX6619571/SRX6619571.05_model.r WARNING @ Tue, 16 Jun 2020 11:46:11: #2 Since the d (211) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 11:46:11: #2 You may need to consider one of the other alternative d(s): 211,225 WARNING @ Tue, 16 Jun 2020 11:46:11: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 11:46:11: #3 Call peaks... INFO @ Tue, 16 Jun 2020 11:46:11: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 11:46:15: 37000000 INFO @ Tue, 16 Jun 2020 11:46:16: 42000000 INFO @ Tue, 16 Jun 2020 11:46:23: 38000000 INFO @ Tue, 16 Jun 2020 11:46:23: 43000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 11:46:31: 44000000 INFO @ Tue, 16 Jun 2020 11:46:31: 39000000 INFO @ Tue, 16 Jun 2020 11:46:38: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 11:46:39: 45000000 INFO @ Tue, 16 Jun 2020 11:46:39: 40000000 INFO @ Tue, 16 Jun 2020 11:46:43: #1 tag size is determined as 150 bps INFO @ Tue, 16 Jun 2020 11:46:43: #1 tag size = 150 INFO @ Tue, 16 Jun 2020 11:46:43: #1 total tags in treatment: 14700099 INFO @ Tue, 16 Jun 2020 11:46:43: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 11:46:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 11:46:43: #1 tags after filtering in treatment: 11339203 INFO @ Tue, 16 Jun 2020 11:46:43: #1 Redundant rate of treatment: 0.23 INFO @ Tue, 16 Jun 2020 11:46:43: #1 finished! INFO @ Tue, 16 Jun 2020 11:46:43: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 11:46:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 11:46:44: #2 number of paired peaks: 451 WARNING @ Tue, 16 Jun 2020 11:46:44: Fewer paired peaks (451) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 451 pairs to build model! INFO @ Tue, 16 Jun 2020 11:46:44: start model_add_line... INFO @ Tue, 16 Jun 2020 11:46:44: start X-correlation... INFO @ Tue, 16 Jun 2020 11:46:44: end of X-cor INFO @ Tue, 16 Jun 2020 11:46:44: #2 finished! INFO @ Tue, 16 Jun 2020 11:46:44: #2 predicted fragment length is 211 bps INFO @ Tue, 16 Jun 2020 11:46:44: #2 alternative fragment length(s) may be 211,225 bps INFO @ Tue, 16 Jun 2020 11:46:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX6619571/SRX6619571.10_model.r WARNING @ Tue, 16 Jun 2020 11:46:44: #2 Since the d (211) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 11:46:44: #2 You may need to consider one of the other alternative d(s): 211,225 WARNING @ Tue, 16 Jun 2020 11:46:44: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 11:46:44: #3 Call peaks... INFO @ Tue, 16 Jun 2020 11:46:44: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 11:46:47: 41000000 INFO @ Tue, 16 Jun 2020 11:46:50: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX6619571/SRX6619571.05_peaks.xls INFO @ Tue, 16 Jun 2020 11:46:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX6619571/SRX6619571.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 11:46:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX6619571/SRX6619571.05_summits.bed INFO @ Tue, 16 Jun 2020 11:46:50: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (1099 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 11:46:55: 42000000 INFO @ Tue, 16 Jun 2020 11:47:03: 43000000 INFO @ Tue, 16 Jun 2020 11:47:11: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 11:47:11: 44000000 INFO @ Tue, 16 Jun 2020 11:47:19: 45000000 INFO @ Tue, 16 Jun 2020 11:47:24: #1 tag size is determined as 150 bps INFO @ Tue, 16 Jun 2020 11:47:24: #1 tag size = 150 INFO @ Tue, 16 Jun 2020 11:47:24: #1 total tags in treatment: 14700099 INFO @ Tue, 16 Jun 2020 11:47:24: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 11:47:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 11:47:24: #1 tags after filtering in treatment: 11339203 INFO @ Tue, 16 Jun 2020 11:47:24: #1 Redundant rate of treatment: 0.23 INFO @ Tue, 16 Jun 2020 11:47:24: #1 finished! INFO @ Tue, 16 Jun 2020 11:47:24: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 11:47:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 11:47:24: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX6619571/SRX6619571.10_peaks.xls INFO @ Tue, 16 Jun 2020 11:47:24: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX6619571/SRX6619571.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 11:47:24: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX6619571/SRX6619571.10_summits.bed INFO @ Tue, 16 Jun 2020 11:47:24: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (543 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 11:47:25: #2 number of paired peaks: 451 WARNING @ Tue, 16 Jun 2020 11:47:25: Fewer paired peaks (451) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 451 pairs to build model! INFO @ Tue, 16 Jun 2020 11:47:25: start model_add_line... INFO @ Tue, 16 Jun 2020 11:47:25: start X-correlation... INFO @ Tue, 16 Jun 2020 11:47:25: end of X-cor INFO @ Tue, 16 Jun 2020 11:47:25: #2 finished! INFO @ Tue, 16 Jun 2020 11:47:25: #2 predicted fragment length is 211 bps INFO @ Tue, 16 Jun 2020 11:47:25: #2 alternative fragment length(s) may be 211,225 bps INFO @ Tue, 16 Jun 2020 11:47:25: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX6619571/SRX6619571.20_model.r WARNING @ Tue, 16 Jun 2020 11:47:25: #2 Since the d (211) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 11:47:25: #2 You may need to consider one of the other alternative d(s): 211,225 WARNING @ Tue, 16 Jun 2020 11:47:25: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 11:47:25: #3 Call peaks... INFO @ Tue, 16 Jun 2020 11:47:25: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 11:47:50: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 11:48:01: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX6619571/SRX6619571.20_peaks.xls INFO @ Tue, 16 Jun 2020 11:48:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX6619571/SRX6619571.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 11:48:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX6619571/SRX6619571.20_summits.bed INFO @ Tue, 16 Jun 2020 11:48:01: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (304 records, 4 fields): 2 millis CompletedMACS2peakCalling