Job ID = 6368857 SRX = SRX6619555 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-06-16T00:40:07 prefetch.2.10.7: 1) Downloading 'SRR9866054'... 2020-06-16T00:40:07 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:57:44 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:57:44 prefetch.2.10.7: 1) 'SRR9866054' was downloaded successfully 2020-06-16T00:57:44 prefetch.2.10.7: 'SRR9866054' has 0 unresolved dependencies Read 43555027 spots for SRR9866054/SRR9866054.sra Written 43555027 spots for SRR9866054/SRR9866054.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:20:45 43555027 reads; of these: 43555027 (100.00%) were paired; of these: 27937513 (64.14%) aligned concordantly 0 times 12830720 (29.46%) aligned concordantly exactly 1 time 2786794 (6.40%) aligned concordantly >1 times ---- 27937513 pairs aligned concordantly 0 times; of these: 14025004 (50.20%) aligned discordantly 1 time ---- 13912509 pairs aligned 0 times concordantly or discordantly; of these: 27825018 mates make up the pairs; of these: 24161246 (86.83%) aligned 0 times 1278940 (4.60%) aligned exactly 1 time 2384832 (8.57%) aligned >1 times 72.26% overall alignment rate Time searching: 01:20:45 Overall time: 01:20:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 48 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 4682442 / 29065309 = 0.1611 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 11:56:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619555/SRX6619555.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619555/SRX6619555.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619555/SRX6619555.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619555/SRX6619555.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 11:56:51: #1 read tag files... INFO @ Tue, 16 Jun 2020 11:56:51: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 11:57:00: 1000000 INFO @ Tue, 16 Jun 2020 11:57:09: 2000000 INFO @ Tue, 16 Jun 2020 11:57:18: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 11:57:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619555/SRX6619555.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619555/SRX6619555.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619555/SRX6619555.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619555/SRX6619555.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 11:57:21: #1 read tag files... INFO @ Tue, 16 Jun 2020 11:57:21: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 11:57:27: 4000000 INFO @ Tue, 16 Jun 2020 11:57:32: 1000000 INFO @ Tue, 16 Jun 2020 11:57:38: 5000000 INFO @ Tue, 16 Jun 2020 11:57:43: 2000000 INFO @ Tue, 16 Jun 2020 11:57:48: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 11:57:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619555/SRX6619555.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619555/SRX6619555.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619555/SRX6619555.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619555/SRX6619555.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 11:57:51: #1 read tag files... INFO @ Tue, 16 Jun 2020 11:57:51: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 11:57:54: 3000000 INFO @ Tue, 16 Jun 2020 11:57:59: 7000000 INFO @ Tue, 16 Jun 2020 11:58:01: 1000000 INFO @ Tue, 16 Jun 2020 11:58:05: 4000000 INFO @ Tue, 16 Jun 2020 11:58:09: 8000000 INFO @ Tue, 16 Jun 2020 11:58:12: 2000000 INFO @ Tue, 16 Jun 2020 11:58:16: 5000000 INFO @ Tue, 16 Jun 2020 11:58:20: 9000000 INFO @ Tue, 16 Jun 2020 11:58:22: 3000000 INFO @ Tue, 16 Jun 2020 11:58:27: 6000000 INFO @ Tue, 16 Jun 2020 11:58:30: 10000000 INFO @ Tue, 16 Jun 2020 11:58:33: 4000000 INFO @ Tue, 16 Jun 2020 11:58:38: 7000000 INFO @ Tue, 16 Jun 2020 11:58:41: 11000000 INFO @ Tue, 16 Jun 2020 11:58:44: 5000000 INFO @ Tue, 16 Jun 2020 11:58:50: 8000000 INFO @ Tue, 16 Jun 2020 11:58:51: 12000000 INFO @ Tue, 16 Jun 2020 11:58:54: 6000000 INFO @ Tue, 16 Jun 2020 11:59:01: 13000000 INFO @ Tue, 16 Jun 2020 11:59:01: 9000000 INFO @ Tue, 16 Jun 2020 11:59:05: 7000000 INFO @ Tue, 16 Jun 2020 11:59:12: 14000000 INFO @ Tue, 16 Jun 2020 11:59:13: 10000000 INFO @ Tue, 16 Jun 2020 11:59:16: 8000000 INFO @ Tue, 16 Jun 2020 11:59:23: 15000000 INFO @ Tue, 16 Jun 2020 11:59:24: 11000000 INFO @ Tue, 16 Jun 2020 11:59:26: 9000000 INFO @ Tue, 16 Jun 2020 11:59:33: 16000000 INFO @ Tue, 16 Jun 2020 11:59:35: 12000000 INFO @ Tue, 16 Jun 2020 11:59:37: 10000000 INFO @ Tue, 16 Jun 2020 11:59:44: 17000000 INFO @ Tue, 16 Jun 2020 11:59:46: 13000000 INFO @ Tue, 16 Jun 2020 11:59:48: 11000000 INFO @ Tue, 16 Jun 2020 11:59:55: 18000000 INFO @ Tue, 16 Jun 2020 11:59:58: 14000000 INFO @ Tue, 16 Jun 2020 11:59:59: 12000000 INFO @ Tue, 16 Jun 2020 12:00:06: 19000000 INFO @ Tue, 16 Jun 2020 12:00:09: 15000000 INFO @ Tue, 16 Jun 2020 12:00:10: 13000000 INFO @ Tue, 16 Jun 2020 12:00:17: 20000000 INFO @ Tue, 16 Jun 2020 12:00:20: 14000000 INFO @ Tue, 16 Jun 2020 12:00:21: 16000000 INFO @ Tue, 16 Jun 2020 12:00:27: 21000000 INFO @ Tue, 16 Jun 2020 12:00:31: 15000000 INFO @ Tue, 16 Jun 2020 12:00:33: 17000000 INFO @ Tue, 16 Jun 2020 12:00:38: 22000000 INFO @ Tue, 16 Jun 2020 12:00:42: 16000000 INFO @ Tue, 16 Jun 2020 12:00:45: 18000000 INFO @ Tue, 16 Jun 2020 12:00:49: 23000000 INFO @ Tue, 16 Jun 2020 12:00:52: 17000000 INFO @ Tue, 16 Jun 2020 12:00:57: 19000000 INFO @ Tue, 16 Jun 2020 12:00:59: 24000000 INFO @ Tue, 16 Jun 2020 12:01:03: 18000000 INFO @ Tue, 16 Jun 2020 12:01:09: 20000000 INFO @ Tue, 16 Jun 2020 12:01:10: 25000000 INFO @ Tue, 16 Jun 2020 12:01:14: 19000000 INFO @ Tue, 16 Jun 2020 12:01:21: 21000000 INFO @ Tue, 16 Jun 2020 12:01:21: 26000000 INFO @ Tue, 16 Jun 2020 12:01:25: 20000000 INFO @ Tue, 16 Jun 2020 12:01:31: 27000000 INFO @ Tue, 16 Jun 2020 12:01:32: 22000000 INFO @ Tue, 16 Jun 2020 12:01:35: 21000000 INFO @ Tue, 16 Jun 2020 12:01:42: 28000000 INFO @ Tue, 16 Jun 2020 12:01:44: 23000000 INFO @ Tue, 16 Jun 2020 12:01:46: 22000000 INFO @ Tue, 16 Jun 2020 12:01:53: 29000000 INFO @ Tue, 16 Jun 2020 12:01:56: 24000000 INFO @ Tue, 16 Jun 2020 12:01:57: 23000000 INFO @ Tue, 16 Jun 2020 12:02:04: 30000000 INFO @ Tue, 16 Jun 2020 12:02:07: 25000000 INFO @ Tue, 16 Jun 2020 12:02:08: 24000000 INFO @ Tue, 16 Jun 2020 12:02:14: 31000000 INFO @ Tue, 16 Jun 2020 12:02:18: 25000000 INFO @ Tue, 16 Jun 2020 12:02:19: 26000000 INFO @ Tue, 16 Jun 2020 12:02:25: 32000000 INFO @ Tue, 16 Jun 2020 12:02:29: 26000000 INFO @ Tue, 16 Jun 2020 12:02:31: 27000000 INFO @ Tue, 16 Jun 2020 12:02:36: 33000000 INFO @ Tue, 16 Jun 2020 12:02:40: 27000000 INFO @ Tue, 16 Jun 2020 12:02:43: 28000000 INFO @ Tue, 16 Jun 2020 12:02:47: 34000000 INFO @ Tue, 16 Jun 2020 12:02:50: 28000000 INFO @ Tue, 16 Jun 2020 12:02:54: 29000000 INFO @ Tue, 16 Jun 2020 12:02:58: 35000000 INFO @ Tue, 16 Jun 2020 12:03:01: 29000000 INFO @ Tue, 16 Jun 2020 12:03:06: 30000000 INFO @ Tue, 16 Jun 2020 12:03:09: 36000000 INFO @ Tue, 16 Jun 2020 12:03:12: 30000000 INFO @ Tue, 16 Jun 2020 12:03:18: 31000000 INFO @ Tue, 16 Jun 2020 12:03:20: 37000000 INFO @ Tue, 16 Jun 2020 12:03:23: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 12:03:29: 32000000 INFO @ Tue, 16 Jun 2020 12:03:30: 38000000 INFO @ Tue, 16 Jun 2020 12:03:33: 32000000 INFO @ Tue, 16 Jun 2020 12:03:41: 39000000 INFO @ Tue, 16 Jun 2020 12:03:41: 33000000 INFO @ Tue, 16 Jun 2020 12:03:44: 33000000 INFO @ Tue, 16 Jun 2020 12:03:52: 40000000 INFO @ Tue, 16 Jun 2020 12:03:52: 34000000 INFO @ Tue, 16 Jun 2020 12:03:55: 34000000 INFO @ Tue, 16 Jun 2020 12:04:03: 41000000 INFO @ Tue, 16 Jun 2020 12:04:04: 35000000 INFO @ Tue, 16 Jun 2020 12:04:05: 35000000 INFO @ Tue, 16 Jun 2020 12:04:13: 42000000 INFO @ Tue, 16 Jun 2020 12:04:15: 36000000 INFO @ Tue, 16 Jun 2020 12:04:16: 36000000 INFO @ Tue, 16 Jun 2020 12:04:24: 43000000 INFO @ Tue, 16 Jun 2020 12:04:27: 37000000 INFO @ Tue, 16 Jun 2020 12:04:28: 37000000 INFO @ Tue, 16 Jun 2020 12:04:35: 44000000 INFO @ Tue, 16 Jun 2020 12:04:38: 38000000 INFO @ Tue, 16 Jun 2020 12:04:39: 38000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 12:04:45: 45000000 INFO @ Tue, 16 Jun 2020 12:04:50: 39000000 INFO @ Tue, 16 Jun 2020 12:04:51: 39000000 INFO @ Tue, 16 Jun 2020 12:04:56: 46000000 INFO @ Tue, 16 Jun 2020 12:05:01: 40000000 INFO @ Tue, 16 Jun 2020 12:05:02: 40000000 INFO @ Tue, 16 Jun 2020 12:05:07: 47000000 INFO @ Tue, 16 Jun 2020 12:05:13: 41000000 INFO @ Tue, 16 Jun 2020 12:05:14: 41000000 INFO @ Tue, 16 Jun 2020 12:05:17: 48000000 INFO @ Tue, 16 Jun 2020 12:05:25: 42000000 INFO @ Tue, 16 Jun 2020 12:05:25: 42000000 INFO @ Tue, 16 Jun 2020 12:05:28: 49000000 INFO @ Tue, 16 Jun 2020 12:05:37: 43000000 INFO @ Tue, 16 Jun 2020 12:05:37: 43000000 INFO @ Tue, 16 Jun 2020 12:05:39: 50000000 INFO @ Tue, 16 Jun 2020 12:05:48: 44000000 INFO @ Tue, 16 Jun 2020 12:05:49: 44000000 INFO @ Tue, 16 Jun 2020 12:05:49: 51000000 INFO @ Tue, 16 Jun 2020 12:06:00: 45000000 INFO @ Tue, 16 Jun 2020 12:06:00: 52000000 INFO @ Tue, 16 Jun 2020 12:06:00: 45000000 INFO @ Tue, 16 Jun 2020 12:06:11: 53000000 INFO @ Tue, 16 Jun 2020 12:06:11: 46000000 INFO @ Tue, 16 Jun 2020 12:06:12: 46000000 INFO @ Tue, 16 Jun 2020 12:06:17: #1 tag size is determined as 150 bps INFO @ Tue, 16 Jun 2020 12:06:17: #1 tag size = 150 INFO @ Tue, 16 Jun 2020 12:06:17: #1 total tags in treatment: 12888218 INFO @ Tue, 16 Jun 2020 12:06:17: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 12:06:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 12:06:18: #1 tags after filtering in treatment: 10822535 INFO @ Tue, 16 Jun 2020 12:06:18: #1 Redundant rate of treatment: 0.16 INFO @ Tue, 16 Jun 2020 12:06:18: #1 finished! INFO @ Tue, 16 Jun 2020 12:06:18: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 12:06:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 12:06:18: #2 number of paired peaks: 299 WARNING @ Tue, 16 Jun 2020 12:06:18: Fewer paired peaks (299) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 299 pairs to build model! INFO @ Tue, 16 Jun 2020 12:06:18: start model_add_line... INFO @ Tue, 16 Jun 2020 12:06:18: start X-correlation... INFO @ Tue, 16 Jun 2020 12:06:18: end of X-cor INFO @ Tue, 16 Jun 2020 12:06:18: #2 finished! INFO @ Tue, 16 Jun 2020 12:06:18: #2 predicted fragment length is 212 bps INFO @ Tue, 16 Jun 2020 12:06:18: #2 alternative fragment length(s) may be 212 bps INFO @ Tue, 16 Jun 2020 12:06:18: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX6619555/SRX6619555.05_model.r WARNING @ Tue, 16 Jun 2020 12:06:18: #2 Since the d (212) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 12:06:18: #2 You may need to consider one of the other alternative d(s): 212 WARNING @ Tue, 16 Jun 2020 12:06:18: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 12:06:18: #3 Call peaks... INFO @ Tue, 16 Jun 2020 12:06:18: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 12:06:23: 47000000 INFO @ Tue, 16 Jun 2020 12:06:24: 47000000 INFO @ Tue, 16 Jun 2020 12:06:35: 48000000 INFO @ Tue, 16 Jun 2020 12:06:35: 48000000 INFO @ Tue, 16 Jun 2020 12:06:43: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 12:06:46: 49000000 INFO @ Tue, 16 Jun 2020 12:06:47: 49000000 INFO @ Tue, 16 Jun 2020 12:06:54: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX6619555/SRX6619555.05_peaks.xls INFO @ Tue, 16 Jun 2020 12:06:54: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX6619555/SRX6619555.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 12:06:54: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX6619555/SRX6619555.05_summits.bed INFO @ Tue, 16 Jun 2020 12:06:54: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (556 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 12:06:58: 50000000 INFO @ Tue, 16 Jun 2020 12:06:58: 50000000 INFO @ Tue, 16 Jun 2020 12:07:09: 51000000 INFO @ Tue, 16 Jun 2020 12:07:10: 51000000 INFO @ Tue, 16 Jun 2020 12:07:20: 52000000 INFO @ Tue, 16 Jun 2020 12:07:21: 52000000 INFO @ Tue, 16 Jun 2020 12:07:32: 53000000 INFO @ Tue, 16 Jun 2020 12:07:32: 53000000 INFO @ Tue, 16 Jun 2020 12:07:38: #1 tag size is determined as 150 bps INFO @ Tue, 16 Jun 2020 12:07:38: #1 tag size = 150 INFO @ Tue, 16 Jun 2020 12:07:38: #1 total tags in treatment: 12888218 INFO @ Tue, 16 Jun 2020 12:07:38: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 12:07:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 12:07:39: #1 tags after filtering in treatment: 10822535 INFO @ Tue, 16 Jun 2020 12:07:39: #1 Redundant rate of treatment: 0.16 INFO @ Tue, 16 Jun 2020 12:07:39: #1 finished! INFO @ Tue, 16 Jun 2020 12:07:39: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 12:07:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 12:07:39: #1 tag size is determined as 150 bps INFO @ Tue, 16 Jun 2020 12:07:39: #1 tag size = 150 INFO @ Tue, 16 Jun 2020 12:07:39: #1 total tags in treatment: 12888218 INFO @ Tue, 16 Jun 2020 12:07:39: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 12:07:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 12:07:39: #1 tags after filtering in treatment: 10822535 INFO @ Tue, 16 Jun 2020 12:07:39: #1 Redundant rate of treatment: 0.16 INFO @ Tue, 16 Jun 2020 12:07:39: #1 finished! INFO @ Tue, 16 Jun 2020 12:07:39: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 12:07:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 12:07:39: #2 number of paired peaks: 299 WARNING @ Tue, 16 Jun 2020 12:07:39: Fewer paired peaks (299) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 299 pairs to build model! INFO @ Tue, 16 Jun 2020 12:07:39: start model_add_line... INFO @ Tue, 16 Jun 2020 12:07:40: start X-correlation... INFO @ Tue, 16 Jun 2020 12:07:40: end of X-cor INFO @ Tue, 16 Jun 2020 12:07:40: #2 finished! INFO @ Tue, 16 Jun 2020 12:07:40: #2 predicted fragment length is 212 bps INFO @ Tue, 16 Jun 2020 12:07:40: #2 alternative fragment length(s) may be 212 bps INFO @ Tue, 16 Jun 2020 12:07:40: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX6619555/SRX6619555.10_model.r WARNING @ Tue, 16 Jun 2020 12:07:40: #2 Since the d (212) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 12:07:40: #2 You may need to consider one of the other alternative d(s): 212 WARNING @ Tue, 16 Jun 2020 12:07:40: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 12:07:40: #3 Call peaks... INFO @ Tue, 16 Jun 2020 12:07:40: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 12:07:40: #2 number of paired peaks: 299 WARNING @ Tue, 16 Jun 2020 12:07:40: Fewer paired peaks (299) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 299 pairs to build model! INFO @ Tue, 16 Jun 2020 12:07:40: start model_add_line... INFO @ Tue, 16 Jun 2020 12:07:40: start X-correlation... INFO @ Tue, 16 Jun 2020 12:07:40: end of X-cor INFO @ Tue, 16 Jun 2020 12:07:40: #2 finished! INFO @ Tue, 16 Jun 2020 12:07:40: #2 predicted fragment length is 212 bps INFO @ Tue, 16 Jun 2020 12:07:40: #2 alternative fragment length(s) may be 212 bps INFO @ Tue, 16 Jun 2020 12:07:40: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX6619555/SRX6619555.20_model.r WARNING @ Tue, 16 Jun 2020 12:07:40: #2 Since the d (212) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 12:07:40: #2 You may need to consider one of the other alternative d(s): 212 WARNING @ Tue, 16 Jun 2020 12:07:40: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 12:07:40: #3 Call peaks... INFO @ Tue, 16 Jun 2020 12:07:40: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 12:08:02: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 12:08:03: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 12:08:13: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX6619555/SRX6619555.10_peaks.xls INFO @ Tue, 16 Jun 2020 12:08:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX6619555/SRX6619555.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 12:08:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX6619555/SRX6619555.10_summits.bed INFO @ Tue, 16 Jun 2020 12:08:13: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (367 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 12:08:14: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX6619555/SRX6619555.20_peaks.xls INFO @ Tue, 16 Jun 2020 12:08:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX6619555/SRX6619555.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 12:08:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX6619555/SRX6619555.20_summits.bed INFO @ Tue, 16 Jun 2020 12:08:14: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (228 records, 4 fields): 1 millis CompletedMACS2peakCalling