Job ID = 6368839 SRX = SRX6619538 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-06-16T00:30:42 prefetch.2.10.7: 1) Downloading 'SRR9866071'... 2020-06-16T00:30:42 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:51:46 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:51:46 prefetch.2.10.7: 1) 'SRR9866071' was downloaded successfully 2020-06-16T00:51:46 prefetch.2.10.7: 'SRR9866071' has 0 unresolved dependencies Read 39995409 spots for SRR9866071/SRR9866071.sra Written 39995409 spots for SRR9866071/SRR9866071.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:42:32 39995409 reads; of these: 39995409 (100.00%) were paired; of these: 20330741 (50.83%) aligned concordantly 0 times 16135706 (40.34%) aligned concordantly exactly 1 time 3528962 (8.82%) aligned concordantly >1 times ---- 20330741 pairs aligned concordantly 0 times; of these: 10207866 (50.21%) aligned discordantly 1 time ---- 10122875 pairs aligned 0 times concordantly or discordantly; of these: 20245750 mates make up the pairs; of these: 16297686 (80.50%) aligned 0 times 1675340 (8.28%) aligned exactly 1 time 2272724 (11.23%) aligned >1 times 79.63% overall alignment rate Time searching: 01:42:32 Overall time: 01:42:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 48 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 4044409 / 29220286 = 0.1384 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 12:17:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619538/SRX6619538.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619538/SRX6619538.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619538/SRX6619538.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619538/SRX6619538.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 12:17:41: #1 read tag files... INFO @ Tue, 16 Jun 2020 12:17:41: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 12:17:49: 1000000 INFO @ Tue, 16 Jun 2020 12:17:58: 2000000 INFO @ Tue, 16 Jun 2020 12:18:06: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 12:18:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619538/SRX6619538.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619538/SRX6619538.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619538/SRX6619538.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619538/SRX6619538.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 12:18:11: #1 read tag files... INFO @ Tue, 16 Jun 2020 12:18:11: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 12:18:16: 4000000 INFO @ Tue, 16 Jun 2020 12:18:20: 1000000 INFO @ Tue, 16 Jun 2020 12:18:26: 5000000 INFO @ Tue, 16 Jun 2020 12:18:31: 2000000 INFO @ Tue, 16 Jun 2020 12:18:37: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 12:18:41: 3000000 INFO @ Tue, 16 Jun 2020 12:18:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619538/SRX6619538.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619538/SRX6619538.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619538/SRX6619538.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619538/SRX6619538.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 12:18:41: #1 read tag files... INFO @ Tue, 16 Jun 2020 12:18:41: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 12:18:48: 7000000 INFO @ Tue, 16 Jun 2020 12:18:50: 1000000 INFO @ Tue, 16 Jun 2020 12:18:50: 4000000 INFO @ Tue, 16 Jun 2020 12:18:59: 5000000 INFO @ Tue, 16 Jun 2020 12:18:59: 8000000 INFO @ Tue, 16 Jun 2020 12:19:00: 2000000 INFO @ Tue, 16 Jun 2020 12:19:08: 6000000 INFO @ Tue, 16 Jun 2020 12:19:09: 9000000 INFO @ Tue, 16 Jun 2020 12:19:11: 3000000 INFO @ Tue, 16 Jun 2020 12:19:16: 7000000 INFO @ Tue, 16 Jun 2020 12:19:19: 10000000 INFO @ Tue, 16 Jun 2020 12:19:22: 4000000 INFO @ Tue, 16 Jun 2020 12:19:25: 8000000 INFO @ Tue, 16 Jun 2020 12:19:30: 11000000 INFO @ Tue, 16 Jun 2020 12:19:31: 5000000 INFO @ Tue, 16 Jun 2020 12:19:34: 9000000 INFO @ Tue, 16 Jun 2020 12:19:40: 12000000 INFO @ Tue, 16 Jun 2020 12:19:42: 6000000 INFO @ Tue, 16 Jun 2020 12:19:43: 10000000 INFO @ Tue, 16 Jun 2020 12:19:50: 13000000 INFO @ Tue, 16 Jun 2020 12:19:51: 11000000 INFO @ Tue, 16 Jun 2020 12:19:52: 7000000 INFO @ Tue, 16 Jun 2020 12:20:00: 12000000 INFO @ Tue, 16 Jun 2020 12:20:01: 14000000 INFO @ Tue, 16 Jun 2020 12:20:03: 8000000 INFO @ Tue, 16 Jun 2020 12:20:09: 13000000 INFO @ Tue, 16 Jun 2020 12:20:11: 15000000 INFO @ Tue, 16 Jun 2020 12:20:13: 9000000 INFO @ Tue, 16 Jun 2020 12:20:18: 14000000 INFO @ Tue, 16 Jun 2020 12:20:22: 16000000 INFO @ Tue, 16 Jun 2020 12:20:23: 10000000 INFO @ Tue, 16 Jun 2020 12:20:28: 15000000 INFO @ Tue, 16 Jun 2020 12:20:32: 17000000 INFO @ Tue, 16 Jun 2020 12:20:34: 11000000 INFO @ Tue, 16 Jun 2020 12:20:37: 16000000 INFO @ Tue, 16 Jun 2020 12:20:43: 18000000 INFO @ Tue, 16 Jun 2020 12:20:44: 12000000 INFO @ Tue, 16 Jun 2020 12:20:46: 17000000 INFO @ Tue, 16 Jun 2020 12:20:53: 19000000 INFO @ Tue, 16 Jun 2020 12:20:54: 13000000 INFO @ Tue, 16 Jun 2020 12:20:55: 18000000 INFO @ Tue, 16 Jun 2020 12:21:04: 20000000 INFO @ Tue, 16 Jun 2020 12:21:04: 19000000 INFO @ Tue, 16 Jun 2020 12:21:04: 14000000 INFO @ Tue, 16 Jun 2020 12:21:13: 20000000 INFO @ Tue, 16 Jun 2020 12:21:14: 21000000 INFO @ Tue, 16 Jun 2020 12:21:14: 15000000 INFO @ Tue, 16 Jun 2020 12:21:22: 21000000 INFO @ Tue, 16 Jun 2020 12:21:24: 16000000 INFO @ Tue, 16 Jun 2020 12:21:25: 22000000 INFO @ Tue, 16 Jun 2020 12:21:32: 22000000 INFO @ Tue, 16 Jun 2020 12:21:34: 17000000 INFO @ Tue, 16 Jun 2020 12:21:35: 23000000 INFO @ Tue, 16 Jun 2020 12:21:41: 23000000 INFO @ Tue, 16 Jun 2020 12:21:44: 18000000 INFO @ Tue, 16 Jun 2020 12:21:45: 24000000 INFO @ Tue, 16 Jun 2020 12:21:50: 24000000 INFO @ Tue, 16 Jun 2020 12:21:54: 19000000 INFO @ Tue, 16 Jun 2020 12:21:55: 25000000 INFO @ Tue, 16 Jun 2020 12:22:00: 25000000 INFO @ Tue, 16 Jun 2020 12:22:04: 20000000 INFO @ Tue, 16 Jun 2020 12:22:06: 26000000 INFO @ Tue, 16 Jun 2020 12:22:09: 26000000 INFO @ Tue, 16 Jun 2020 12:22:14: 21000000 INFO @ Tue, 16 Jun 2020 12:22:16: 27000000 INFO @ Tue, 16 Jun 2020 12:22:18: 27000000 INFO @ Tue, 16 Jun 2020 12:22:24: 22000000 INFO @ Tue, 16 Jun 2020 12:22:26: 28000000 INFO @ Tue, 16 Jun 2020 12:22:27: 28000000 INFO @ Tue, 16 Jun 2020 12:22:34: 23000000 INFO @ Tue, 16 Jun 2020 12:22:36: 29000000 INFO @ Tue, 16 Jun 2020 12:22:36: 29000000 INFO @ Tue, 16 Jun 2020 12:22:44: 24000000 INFO @ Tue, 16 Jun 2020 12:22:45: 30000000 INFO @ Tue, 16 Jun 2020 12:22:46: 30000000 INFO @ Tue, 16 Jun 2020 12:22:54: 31000000 INFO @ Tue, 16 Jun 2020 12:22:54: 25000000 INFO @ Tue, 16 Jun 2020 12:22:56: 31000000 INFO @ Tue, 16 Jun 2020 12:23:03: 32000000 INFO @ Tue, 16 Jun 2020 12:23:04: 26000000 INFO @ Tue, 16 Jun 2020 12:23:06: 32000000 INFO @ Tue, 16 Jun 2020 12:23:11: 33000000 INFO @ Tue, 16 Jun 2020 12:23:14: 27000000 INFO @ Tue, 16 Jun 2020 12:23:16: 33000000 INFO @ Tue, 16 Jun 2020 12:23:20: 34000000 INFO @ Tue, 16 Jun 2020 12:23:24: 28000000 INFO @ Tue, 16 Jun 2020 12:23:26: 34000000 INFO @ Tue, 16 Jun 2020 12:23:29: 35000000 INFO @ Tue, 16 Jun 2020 12:23:33: 29000000 INFO @ Tue, 16 Jun 2020 12:23:36: 35000000 INFO @ Tue, 16 Jun 2020 12:23:38: 36000000 INFO @ Tue, 16 Jun 2020 12:23:43: 30000000 INFO @ Tue, 16 Jun 2020 12:23:45: 36000000 INFO @ Tue, 16 Jun 2020 12:23:47: 37000000 INFO @ Tue, 16 Jun 2020 12:23:53: 31000000 INFO @ Tue, 16 Jun 2020 12:23:55: 37000000 INFO @ Tue, 16 Jun 2020 12:23:56: 38000000 INFO @ Tue, 16 Jun 2020 12:24:03: 32000000 INFO @ Tue, 16 Jun 2020 12:24:05: 39000000 INFO @ Tue, 16 Jun 2020 12:24:05: 38000000 INFO @ Tue, 16 Jun 2020 12:24:13: 33000000 INFO @ Tue, 16 Jun 2020 12:24:14: 40000000 INFO @ Tue, 16 Jun 2020 12:24:15: 39000000 INFO @ Tue, 16 Jun 2020 12:24:23: 34000000 INFO @ Tue, 16 Jun 2020 12:24:23: 41000000 INFO @ Tue, 16 Jun 2020 12:24:26: 40000000 INFO @ Tue, 16 Jun 2020 12:24:32: 42000000 INFO @ Tue, 16 Jun 2020 12:24:33: 35000000 INFO @ Tue, 16 Jun 2020 12:24:36: 41000000 INFO @ Tue, 16 Jun 2020 12:24:41: 43000000 INFO @ Tue, 16 Jun 2020 12:24:43: 36000000 INFO @ Tue, 16 Jun 2020 12:24:46: 42000000 INFO @ Tue, 16 Jun 2020 12:24:50: 44000000 INFO @ Tue, 16 Jun 2020 12:24:52: 37000000 INFO @ Tue, 16 Jun 2020 12:24:57: 43000000 INFO @ Tue, 16 Jun 2020 12:24:59: 45000000 INFO @ Tue, 16 Jun 2020 12:25:02: 38000000 INFO @ Tue, 16 Jun 2020 12:25:07: 44000000 INFO @ Tue, 16 Jun 2020 12:25:08: 46000000 INFO @ Tue, 16 Jun 2020 12:25:12: 39000000 INFO @ Tue, 16 Jun 2020 12:25:17: 47000000 INFO @ Tue, 16 Jun 2020 12:25:17: 45000000 INFO @ Tue, 16 Jun 2020 12:25:21: 40000000 INFO @ Tue, 16 Jun 2020 12:25:26: 48000000 INFO @ Tue, 16 Jun 2020 12:25:28: 46000000 INFO @ Tue, 16 Jun 2020 12:25:31: 41000000 INFO @ Tue, 16 Jun 2020 12:25:35: 49000000 INFO @ Tue, 16 Jun 2020 12:25:38: 47000000 INFO @ Tue, 16 Jun 2020 12:25:41: 42000000 INFO @ Tue, 16 Jun 2020 12:25:44: 50000000 INFO @ Tue, 16 Jun 2020 12:25:48: 48000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 12:25:51: 43000000 INFO @ Tue, 16 Jun 2020 12:25:53: 51000000 INFO @ Tue, 16 Jun 2020 12:25:59: 49000000 INFO @ Tue, 16 Jun 2020 12:26:01: 52000000 INFO @ Tue, 16 Jun 2020 12:26:02: 44000000 INFO @ Tue, 16 Jun 2020 12:26:08: 50000000 INFO @ Tue, 16 Jun 2020 12:26:10: 53000000 INFO @ Tue, 16 Jun 2020 12:26:11: 45000000 INFO @ Tue, 16 Jun 2020 12:26:18: 51000000 INFO @ Tue, 16 Jun 2020 12:26:19: 54000000 INFO @ Tue, 16 Jun 2020 12:26:21: 46000000 INFO @ Tue, 16 Jun 2020 12:26:27: 55000000 INFO @ Tue, 16 Jun 2020 12:26:27: 52000000 INFO @ Tue, 16 Jun 2020 12:26:31: 47000000 INFO @ Tue, 16 Jun 2020 12:26:33: #1 tag size is determined as 150 bps INFO @ Tue, 16 Jun 2020 12:26:33: #1 tag size = 150 INFO @ Tue, 16 Jun 2020 12:26:33: #1 total tags in treatment: 16738223 INFO @ Tue, 16 Jun 2020 12:26:33: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 12:26:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 12:26:33: #1 tags after filtering in treatment: 13758066 INFO @ Tue, 16 Jun 2020 12:26:33: #1 Redundant rate of treatment: 0.18 INFO @ Tue, 16 Jun 2020 12:26:33: #1 finished! INFO @ Tue, 16 Jun 2020 12:26:33: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 12:26:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 12:26:34: #2 number of paired peaks: 247 WARNING @ Tue, 16 Jun 2020 12:26:34: Fewer paired peaks (247) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 247 pairs to build model! INFO @ Tue, 16 Jun 2020 12:26:34: start model_add_line... INFO @ Tue, 16 Jun 2020 12:26:34: start X-correlation... INFO @ Tue, 16 Jun 2020 12:26:34: end of X-cor INFO @ Tue, 16 Jun 2020 12:26:34: #2 finished! INFO @ Tue, 16 Jun 2020 12:26:34: #2 predicted fragment length is 197 bps INFO @ Tue, 16 Jun 2020 12:26:34: #2 alternative fragment length(s) may be 197,212 bps INFO @ Tue, 16 Jun 2020 12:26:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX6619538/SRX6619538.10_model.r WARNING @ Tue, 16 Jun 2020 12:26:34: #2 Since the d (197) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 12:26:34: #2 You may need to consider one of the other alternative d(s): 197,212 WARNING @ Tue, 16 Jun 2020 12:26:34: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 12:26:34: #3 Call peaks... INFO @ Tue, 16 Jun 2020 12:26:34: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 12:26:37: 53000000 INFO @ Tue, 16 Jun 2020 12:26:40: 48000000 INFO @ Tue, 16 Jun 2020 12:26:46: 54000000 INFO @ Tue, 16 Jun 2020 12:26:49: 49000000 INFO @ Tue, 16 Jun 2020 12:26:56: 55000000 INFO @ Tue, 16 Jun 2020 12:26:59: 50000000 INFO @ Tue, 16 Jun 2020 12:27:01: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 12:27:02: #1 tag size is determined as 150 bps INFO @ Tue, 16 Jun 2020 12:27:02: #1 tag size = 150 INFO @ Tue, 16 Jun 2020 12:27:02: #1 total tags in treatment: 16738223 INFO @ Tue, 16 Jun 2020 12:27:02: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 12:27:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 12:27:02: #1 tags after filtering in treatment: 13758066 INFO @ Tue, 16 Jun 2020 12:27:02: #1 Redundant rate of treatment: 0.18 INFO @ Tue, 16 Jun 2020 12:27:02: #1 finished! INFO @ Tue, 16 Jun 2020 12:27:02: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 12:27:02: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 12:27:03: #2 number of paired peaks: 247 WARNING @ Tue, 16 Jun 2020 12:27:03: Fewer paired peaks (247) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 247 pairs to build model! INFO @ Tue, 16 Jun 2020 12:27:03: start model_add_line... INFO @ Tue, 16 Jun 2020 12:27:03: start X-correlation... INFO @ Tue, 16 Jun 2020 12:27:03: end of X-cor INFO @ Tue, 16 Jun 2020 12:27:03: #2 finished! INFO @ Tue, 16 Jun 2020 12:27:03: #2 predicted fragment length is 197 bps INFO @ Tue, 16 Jun 2020 12:27:03: #2 alternative fragment length(s) may be 197,212 bps INFO @ Tue, 16 Jun 2020 12:27:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX6619538/SRX6619538.05_model.r WARNING @ Tue, 16 Jun 2020 12:27:03: #2 Since the d (197) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 12:27:03: #2 You may need to consider one of the other alternative d(s): 197,212 WARNING @ Tue, 16 Jun 2020 12:27:03: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 12:27:03: #3 Call peaks... INFO @ Tue, 16 Jun 2020 12:27:03: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 12:27:07: 51000000 INFO @ Tue, 16 Jun 2020 12:27:14: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX6619538/SRX6619538.10_peaks.xls INFO @ Tue, 16 Jun 2020 12:27:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX6619538/SRX6619538.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 12:27:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX6619538/SRX6619538.10_summits.bed INFO @ Tue, 16 Jun 2020 12:27:14: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (392 records, 4 fields): 70 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 12:27:16: 52000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 12:27:24: 53000000 INFO @ Tue, 16 Jun 2020 12:27:31: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 12:27:33: 54000000 INFO @ Tue, 16 Jun 2020 12:27:41: 55000000 INFO @ Tue, 16 Jun 2020 12:27:44: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX6619538/SRX6619538.05_peaks.xls INFO @ Tue, 16 Jun 2020 12:27:44: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX6619538/SRX6619538.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 12:27:44: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX6619538/SRX6619538.05_summits.bed INFO @ Tue, 16 Jun 2020 12:27:44: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (647 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 12:27:46: #1 tag size is determined as 150 bps INFO @ Tue, 16 Jun 2020 12:27:46: #1 tag size = 150 INFO @ Tue, 16 Jun 2020 12:27:46: #1 total tags in treatment: 16738223 INFO @ Tue, 16 Jun 2020 12:27:46: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 12:27:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 12:27:46: #1 tags after filtering in treatment: 13758066 INFO @ Tue, 16 Jun 2020 12:27:46: #1 Redundant rate of treatment: 0.18 INFO @ Tue, 16 Jun 2020 12:27:46: #1 finished! INFO @ Tue, 16 Jun 2020 12:27:46: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 12:27:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 12:27:47: #2 number of paired peaks: 247 WARNING @ Tue, 16 Jun 2020 12:27:47: Fewer paired peaks (247) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 247 pairs to build model! INFO @ Tue, 16 Jun 2020 12:27:47: start model_add_line... INFO @ Tue, 16 Jun 2020 12:27:47: start X-correlation... INFO @ Tue, 16 Jun 2020 12:27:47: end of X-cor INFO @ Tue, 16 Jun 2020 12:27:47: #2 finished! INFO @ Tue, 16 Jun 2020 12:27:47: #2 predicted fragment length is 197 bps INFO @ Tue, 16 Jun 2020 12:27:47: #2 alternative fragment length(s) may be 197,212 bps INFO @ Tue, 16 Jun 2020 12:27:47: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX6619538/SRX6619538.20_model.r WARNING @ Tue, 16 Jun 2020 12:27:47: #2 Since the d (197) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 12:27:47: #2 You may need to consider one of the other alternative d(s): 197,212 WARNING @ Tue, 16 Jun 2020 12:27:47: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 12:27:47: #3 Call peaks... INFO @ Tue, 16 Jun 2020 12:27:47: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 12:28:14: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 12:28:26: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX6619538/SRX6619538.20_peaks.xls INFO @ Tue, 16 Jun 2020 12:28:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX6619538/SRX6619538.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 12:28:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX6619538/SRX6619538.20_summits.bed INFO @ Tue, 16 Jun 2020 12:28:26: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (237 records, 4 fields): 1 millis CompletedMACS2peakCalling