Job ID = 6368836 SRX = SRX6619535 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-06-16T00:35:16 prefetch.2.10.7: 1) Downloading 'SRR9866073'... 2020-06-16T00:35:16 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:52:04 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:52:04 prefetch.2.10.7: 1) 'SRR9866073' was downloaded successfully 2020-06-16T00:52:04 prefetch.2.10.7: 'SRR9866073' has 0 unresolved dependencies Read 39751115 spots for SRR9866073/SRR9866073.sra Written 39751115 spots for SRR9866073/SRR9866073.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:11:34 39751115 reads; of these: 39751115 (100.00%) were paired; of these: 24879506 (62.59%) aligned concordantly 0 times 12264404 (30.85%) aligned concordantly exactly 1 time 2607205 (6.56%) aligned concordantly >1 times ---- 24879506 pairs aligned concordantly 0 times; of these: 12311783 (49.49%) aligned discordantly 1 time ---- 12567723 pairs aligned 0 times concordantly or discordantly; of these: 25135446 mates make up the pairs; of these: 21855423 (86.95%) aligned 0 times 1138410 (4.53%) aligned exactly 1 time 2141613 (8.52%) aligned >1 times 72.51% overall alignment rate Time searching: 01:11:34 Overall time: 01:11:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 44 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 4154271 / 26638996 = 0.1559 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 11:41:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619535/SRX6619535.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619535/SRX6619535.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619535/SRX6619535.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619535/SRX6619535.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 11:41:31: #1 read tag files... INFO @ Tue, 16 Jun 2020 11:41:31: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 11:41:39: 1000000 INFO @ Tue, 16 Jun 2020 11:41:48: 2000000 INFO @ Tue, 16 Jun 2020 11:41:56: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 11:42:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619535/SRX6619535.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619535/SRX6619535.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619535/SRX6619535.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619535/SRX6619535.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 11:42:01: #1 read tag files... INFO @ Tue, 16 Jun 2020 11:42:01: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 11:42:05: 4000000 INFO @ Tue, 16 Jun 2020 11:42:10: 1000000 INFO @ Tue, 16 Jun 2020 11:42:15: 5000000 INFO @ Tue, 16 Jun 2020 11:42:20: 2000000 INFO @ Tue, 16 Jun 2020 11:42:24: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 11:42:29: 3000000 INFO @ Tue, 16 Jun 2020 11:42:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619535/SRX6619535.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619535/SRX6619535.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619535/SRX6619535.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619535/SRX6619535.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 11:42:31: #1 read tag files... INFO @ Tue, 16 Jun 2020 11:42:31: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 11:42:35: 7000000 INFO @ Tue, 16 Jun 2020 11:42:41: 4000000 INFO @ Tue, 16 Jun 2020 11:42:44: 1000000 INFO @ Tue, 16 Jun 2020 11:42:46: 8000000 INFO @ Tue, 16 Jun 2020 11:42:52: 5000000 INFO @ Tue, 16 Jun 2020 11:42:56: 2000000 INFO @ Tue, 16 Jun 2020 11:42:58: 9000000 INFO @ Tue, 16 Jun 2020 11:43:04: 6000000 INFO @ Tue, 16 Jun 2020 11:43:09: 3000000 INFO @ Tue, 16 Jun 2020 11:43:09: 10000000 INFO @ Tue, 16 Jun 2020 11:43:15: 7000000 INFO @ Tue, 16 Jun 2020 11:43:20: 11000000 INFO @ Tue, 16 Jun 2020 11:43:21: 4000000 INFO @ Tue, 16 Jun 2020 11:43:26: 8000000 INFO @ Tue, 16 Jun 2020 11:43:32: 12000000 INFO @ Tue, 16 Jun 2020 11:43:33: 5000000 INFO @ Tue, 16 Jun 2020 11:43:37: 9000000 INFO @ Tue, 16 Jun 2020 11:43:42: 13000000 INFO @ Tue, 16 Jun 2020 11:43:44: 6000000 INFO @ Tue, 16 Jun 2020 11:43:48: 10000000 INFO @ Tue, 16 Jun 2020 11:43:53: 14000000 INFO @ Tue, 16 Jun 2020 11:43:56: 7000000 INFO @ Tue, 16 Jun 2020 11:43:59: 11000000 INFO @ Tue, 16 Jun 2020 11:44:04: 15000000 INFO @ Tue, 16 Jun 2020 11:44:07: 8000000 INFO @ Tue, 16 Jun 2020 11:44:10: 12000000 INFO @ Tue, 16 Jun 2020 11:44:15: 16000000 INFO @ Tue, 16 Jun 2020 11:44:18: 9000000 INFO @ Tue, 16 Jun 2020 11:44:20: 13000000 INFO @ Tue, 16 Jun 2020 11:44:25: 17000000 INFO @ Tue, 16 Jun 2020 11:44:29: 10000000 INFO @ Tue, 16 Jun 2020 11:44:31: 14000000 INFO @ Tue, 16 Jun 2020 11:44:36: 18000000 INFO @ Tue, 16 Jun 2020 11:44:41: 11000000 INFO @ Tue, 16 Jun 2020 11:44:42: 15000000 INFO @ Tue, 16 Jun 2020 11:44:47: 19000000 INFO @ Tue, 16 Jun 2020 11:44:52: 12000000 INFO @ Tue, 16 Jun 2020 11:44:52: 16000000 INFO @ Tue, 16 Jun 2020 11:44:57: 20000000 INFO @ Tue, 16 Jun 2020 11:45:03: 13000000 INFO @ Tue, 16 Jun 2020 11:45:03: 17000000 INFO @ Tue, 16 Jun 2020 11:45:08: 21000000 INFO @ Tue, 16 Jun 2020 11:45:14: 18000000 INFO @ Tue, 16 Jun 2020 11:45:14: 14000000 INFO @ Tue, 16 Jun 2020 11:45:19: 22000000 INFO @ Tue, 16 Jun 2020 11:45:24: 19000000 INFO @ Tue, 16 Jun 2020 11:45:25: 15000000 INFO @ Tue, 16 Jun 2020 11:45:29: 23000000 INFO @ Tue, 16 Jun 2020 11:45:35: 20000000 INFO @ Tue, 16 Jun 2020 11:45:36: 16000000 INFO @ Tue, 16 Jun 2020 11:45:40: 24000000 INFO @ Tue, 16 Jun 2020 11:45:46: 21000000 INFO @ Tue, 16 Jun 2020 11:45:47: 17000000 INFO @ Tue, 16 Jun 2020 11:45:51: 25000000 INFO @ Tue, 16 Jun 2020 11:45:56: 22000000 INFO @ Tue, 16 Jun 2020 11:45:58: 18000000 INFO @ Tue, 16 Jun 2020 11:46:01: 26000000 INFO @ Tue, 16 Jun 2020 11:46:07: 23000000 INFO @ Tue, 16 Jun 2020 11:46:10: 19000000 INFO @ Tue, 16 Jun 2020 11:46:12: 27000000 INFO @ Tue, 16 Jun 2020 11:46:17: 24000000 INFO @ Tue, 16 Jun 2020 11:46:21: 20000000 INFO @ Tue, 16 Jun 2020 11:46:22: 28000000 INFO @ Tue, 16 Jun 2020 11:46:28: 25000000 INFO @ Tue, 16 Jun 2020 11:46:32: 21000000 INFO @ Tue, 16 Jun 2020 11:46:33: 29000000 INFO @ Tue, 16 Jun 2020 11:46:38: 26000000 INFO @ Tue, 16 Jun 2020 11:46:43: 22000000 INFO @ Tue, 16 Jun 2020 11:46:43: 30000000 INFO @ Tue, 16 Jun 2020 11:46:49: 27000000 INFO @ Tue, 16 Jun 2020 11:46:54: 23000000 INFO @ Tue, 16 Jun 2020 11:46:54: 31000000 INFO @ Tue, 16 Jun 2020 11:46:59: 28000000 INFO @ Tue, 16 Jun 2020 11:47:04: 32000000 INFO @ Tue, 16 Jun 2020 11:47:05: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 11:47:10: 29000000 INFO @ Tue, 16 Jun 2020 11:47:15: 33000000 INFO @ Tue, 16 Jun 2020 11:47:16: 25000000 INFO @ Tue, 16 Jun 2020 11:47:20: 30000000 INFO @ Tue, 16 Jun 2020 11:47:25: 34000000 INFO @ Tue, 16 Jun 2020 11:47:28: 26000000 INFO @ Tue, 16 Jun 2020 11:47:31: 31000000 INFO @ Tue, 16 Jun 2020 11:47:36: 35000000 INFO @ Tue, 16 Jun 2020 11:47:39: 27000000 INFO @ Tue, 16 Jun 2020 11:47:41: 32000000 INFO @ Tue, 16 Jun 2020 11:47:46: 36000000 INFO @ Tue, 16 Jun 2020 11:47:50: 28000000 INFO @ Tue, 16 Jun 2020 11:47:51: 33000000 INFO @ Tue, 16 Jun 2020 11:47:56: 37000000 INFO @ Tue, 16 Jun 2020 11:48:01: 29000000 INFO @ Tue, 16 Jun 2020 11:48:02: 34000000 INFO @ Tue, 16 Jun 2020 11:48:07: 38000000 INFO @ Tue, 16 Jun 2020 11:48:12: 35000000 INFO @ Tue, 16 Jun 2020 11:48:13: 30000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 11:48:17: 39000000 INFO @ Tue, 16 Jun 2020 11:48:23: 36000000 INFO @ Tue, 16 Jun 2020 11:48:24: 31000000 INFO @ Tue, 16 Jun 2020 11:48:28: 40000000 INFO @ Tue, 16 Jun 2020 11:48:34: 37000000 INFO @ Tue, 16 Jun 2020 11:48:35: 32000000 INFO @ Tue, 16 Jun 2020 11:48:39: 41000000 INFO @ Tue, 16 Jun 2020 11:48:44: 38000000 INFO @ Tue, 16 Jun 2020 11:48:46: 33000000 INFO @ Tue, 16 Jun 2020 11:48:49: 42000000 INFO @ Tue, 16 Jun 2020 11:48:55: 39000000 INFO @ Tue, 16 Jun 2020 11:48:57: 34000000 INFO @ Tue, 16 Jun 2020 11:49:00: 43000000 INFO @ Tue, 16 Jun 2020 11:49:06: 40000000 INFO @ Tue, 16 Jun 2020 11:49:08: 35000000 INFO @ Tue, 16 Jun 2020 11:49:10: 44000000 INFO @ Tue, 16 Jun 2020 11:49:16: 41000000 INFO @ Tue, 16 Jun 2020 11:49:19: 36000000 INFO @ Tue, 16 Jun 2020 11:49:21: 45000000 INFO @ Tue, 16 Jun 2020 11:49:27: 42000000 INFO @ Tue, 16 Jun 2020 11:49:30: 37000000 INFO @ Tue, 16 Jun 2020 11:49:31: 46000000 INFO @ Tue, 16 Jun 2020 11:49:37: 43000000 INFO @ Tue, 16 Jun 2020 11:49:42: 38000000 INFO @ Tue, 16 Jun 2020 11:49:42: 47000000 INFO @ Tue, 16 Jun 2020 11:49:48: 44000000 INFO @ Tue, 16 Jun 2020 11:49:52: 48000000 INFO @ Tue, 16 Jun 2020 11:49:53: 39000000 INFO @ Tue, 16 Jun 2020 11:49:58: 45000000 INFO @ Tue, 16 Jun 2020 11:50:03: 49000000 INFO @ Tue, 16 Jun 2020 11:50:04: 40000000 INFO @ Tue, 16 Jun 2020 11:50:06: #1 tag size is determined as 150 bps INFO @ Tue, 16 Jun 2020 11:50:06: #1 tag size = 150 INFO @ Tue, 16 Jun 2020 11:50:06: #1 total tags in treatment: 12357957 INFO @ Tue, 16 Jun 2020 11:50:06: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 11:50:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 11:50:07: #1 tags after filtering in treatment: 10443190 INFO @ Tue, 16 Jun 2020 11:50:07: #1 Redundant rate of treatment: 0.15 INFO @ Tue, 16 Jun 2020 11:50:07: #1 finished! INFO @ Tue, 16 Jun 2020 11:50:07: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 11:50:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 11:50:07: #2 number of paired peaks: 311 WARNING @ Tue, 16 Jun 2020 11:50:07: Fewer paired peaks (311) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 311 pairs to build model! INFO @ Tue, 16 Jun 2020 11:50:07: start model_add_line... INFO @ Tue, 16 Jun 2020 11:50:08: start X-correlation... INFO @ Tue, 16 Jun 2020 11:50:08: end of X-cor INFO @ Tue, 16 Jun 2020 11:50:08: #2 finished! INFO @ Tue, 16 Jun 2020 11:50:08: #2 predicted fragment length is 218 bps INFO @ Tue, 16 Jun 2020 11:50:08: #2 alternative fragment length(s) may be 218 bps INFO @ Tue, 16 Jun 2020 11:50:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX6619535/SRX6619535.05_model.r WARNING @ Tue, 16 Jun 2020 11:50:08: #2 Since the d (218) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 11:50:08: #2 You may need to consider one of the other alternative d(s): 218 WARNING @ Tue, 16 Jun 2020 11:50:08: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 11:50:08: #3 Call peaks... INFO @ Tue, 16 Jun 2020 11:50:08: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 11:50:09: 46000000 INFO @ Tue, 16 Jun 2020 11:50:15: 41000000 INFO @ Tue, 16 Jun 2020 11:50:19: 47000000 INFO @ Tue, 16 Jun 2020 11:50:27: 42000000 INFO @ Tue, 16 Jun 2020 11:50:28: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 11:50:29: 48000000 INFO @ Tue, 16 Jun 2020 11:50:38: 43000000 INFO @ Tue, 16 Jun 2020 11:50:38: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX6619535/SRX6619535.05_peaks.xls INFO @ Tue, 16 Jun 2020 11:50:38: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX6619535/SRX6619535.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 11:50:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX6619535/SRX6619535.05_summits.bed INFO @ Tue, 16 Jun 2020 11:50:38: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (514 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 11:50:39: 49000000 INFO @ Tue, 16 Jun 2020 11:50:42: #1 tag size is determined as 150 bps INFO @ Tue, 16 Jun 2020 11:50:42: #1 tag size = 150 INFO @ Tue, 16 Jun 2020 11:50:42: #1 total tags in treatment: 12357957 INFO @ Tue, 16 Jun 2020 11:50:42: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 11:50:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 11:50:42: #1 tags after filtering in treatment: 10443190 INFO @ Tue, 16 Jun 2020 11:50:42: #1 Redundant rate of treatment: 0.15 INFO @ Tue, 16 Jun 2020 11:50:42: #1 finished! INFO @ Tue, 16 Jun 2020 11:50:42: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 11:50:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 11:50:43: #2 number of paired peaks: 311 WARNING @ Tue, 16 Jun 2020 11:50:43: Fewer paired peaks (311) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 311 pairs to build model! INFO @ Tue, 16 Jun 2020 11:50:43: start model_add_line... INFO @ Tue, 16 Jun 2020 11:50:43: start X-correlation... INFO @ Tue, 16 Jun 2020 11:50:43: end of X-cor INFO @ Tue, 16 Jun 2020 11:50:43: #2 finished! INFO @ Tue, 16 Jun 2020 11:50:43: #2 predicted fragment length is 218 bps INFO @ Tue, 16 Jun 2020 11:50:43: #2 alternative fragment length(s) may be 218 bps INFO @ Tue, 16 Jun 2020 11:50:43: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX6619535/SRX6619535.10_model.r WARNING @ Tue, 16 Jun 2020 11:50:43: #2 Since the d (218) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 11:50:43: #2 You may need to consider one of the other alternative d(s): 218 WARNING @ Tue, 16 Jun 2020 11:50:43: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 11:50:43: #3 Call peaks... INFO @ Tue, 16 Jun 2020 11:50:43: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 11:50:48: 44000000 INFO @ Tue, 16 Jun 2020 11:50:58: 45000000 INFO @ Tue, 16 Jun 2020 11:51:04: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 11:51:08: 46000000 INFO @ Tue, 16 Jun 2020 11:51:14: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX6619535/SRX6619535.10_peaks.xls INFO @ Tue, 16 Jun 2020 11:51:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX6619535/SRX6619535.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 11:51:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX6619535/SRX6619535.10_summits.bed INFO @ Tue, 16 Jun 2020 11:51:14: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (342 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 11:51:18: 47000000 INFO @ Tue, 16 Jun 2020 11:51:28: 48000000 INFO @ Tue, 16 Jun 2020 11:51:38: 49000000 INFO @ Tue, 16 Jun 2020 11:51:41: #1 tag size is determined as 150 bps INFO @ Tue, 16 Jun 2020 11:51:41: #1 tag size = 150 INFO @ Tue, 16 Jun 2020 11:51:41: #1 total tags in treatment: 12357957 INFO @ Tue, 16 Jun 2020 11:51:41: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 11:51:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 11:51:42: #1 tags after filtering in treatment: 10443190 INFO @ Tue, 16 Jun 2020 11:51:42: #1 Redundant rate of treatment: 0.15 INFO @ Tue, 16 Jun 2020 11:51:42: #1 finished! INFO @ Tue, 16 Jun 2020 11:51:42: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 11:51:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 11:51:42: #2 number of paired peaks: 311 WARNING @ Tue, 16 Jun 2020 11:51:42: Fewer paired peaks (311) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 311 pairs to build model! INFO @ Tue, 16 Jun 2020 11:51:42: start model_add_line... INFO @ Tue, 16 Jun 2020 11:51:42: start X-correlation... INFO @ Tue, 16 Jun 2020 11:51:42: end of X-cor INFO @ Tue, 16 Jun 2020 11:51:42: #2 finished! INFO @ Tue, 16 Jun 2020 11:51:42: #2 predicted fragment length is 218 bps INFO @ Tue, 16 Jun 2020 11:51:42: #2 alternative fragment length(s) may be 218 bps INFO @ Tue, 16 Jun 2020 11:51:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX6619535/SRX6619535.20_model.r WARNING @ Tue, 16 Jun 2020 11:51:42: #2 Since the d (218) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 11:51:42: #2 You may need to consider one of the other alternative d(s): 218 WARNING @ Tue, 16 Jun 2020 11:51:42: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 11:51:42: #3 Call peaks... INFO @ Tue, 16 Jun 2020 11:51:42: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 11:52:03: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 11:52:13: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX6619535/SRX6619535.20_peaks.xls INFO @ Tue, 16 Jun 2020 11:52:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX6619535/SRX6619535.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 11:52:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX6619535/SRX6619535.20_summits.bed INFO @ Tue, 16 Jun 2020 11:52:13: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (214 records, 4 fields): 1 millis CompletedMACS2peakCalling