Job ID = 6368833 SRX = SRX6619532 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-06-16T00:38:01 prefetch.2.10.7: 1) Downloading 'SRR9866077'... 2020-06-16T00:38:01 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:57:23 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:57:23 prefetch.2.10.7: 1) 'SRR9866077' was downloaded successfully 2020-06-16T00:57:23 prefetch.2.10.7: 'SRR9866077' has 0 unresolved dependencies Read 36263918 spots for SRR9866077/SRR9866077.sra Written 36263918 spots for SRR9866077/SRR9866077.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:17:09 36263918 reads; of these: 36263918 (100.00%) were paired; of these: 15959065 (44.01%) aligned concordantly 0 times 16929837 (46.69%) aligned concordantly exactly 1 time 3375016 (9.31%) aligned concordantly >1 times ---- 15959065 pairs aligned concordantly 0 times; of these: 9028103 (56.57%) aligned discordantly 1 time ---- 6930962 pairs aligned 0 times concordantly or discordantly; of these: 13861924 mates make up the pairs; of these: 10946558 (78.97%) aligned 0 times 1110247 (8.01%) aligned exactly 1 time 1805119 (13.02%) aligned >1 times 84.91% overall alignment rate Time searching: 01:17:09 Overall time: 01:17:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 48 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 3929731 / 28812885 = 0.1364 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 11:49:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619532/SRX6619532.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619532/SRX6619532.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619532/SRX6619532.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619532/SRX6619532.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 11:49:48: #1 read tag files... INFO @ Tue, 16 Jun 2020 11:49:48: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 11:49:56: 1000000 INFO @ Tue, 16 Jun 2020 11:50:04: 2000000 INFO @ Tue, 16 Jun 2020 11:50:12: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 11:50:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619532/SRX6619532.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619532/SRX6619532.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619532/SRX6619532.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619532/SRX6619532.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 11:50:18: #1 read tag files... INFO @ Tue, 16 Jun 2020 11:50:18: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 11:50:20: 4000000 INFO @ Tue, 16 Jun 2020 11:50:27: 1000000 INFO @ Tue, 16 Jun 2020 11:50:29: 5000000 INFO @ Tue, 16 Jun 2020 11:50:36: 2000000 INFO @ Tue, 16 Jun 2020 11:50:37: 6000000 INFO @ Tue, 16 Jun 2020 11:50:45: 3000000 INFO @ Tue, 16 Jun 2020 11:50:46: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 11:50:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619532/SRX6619532.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619532/SRX6619532.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619532/SRX6619532.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619532/SRX6619532.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 11:50:48: #1 read tag files... INFO @ Tue, 16 Jun 2020 11:50:48: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 11:50:54: 4000000 INFO @ Tue, 16 Jun 2020 11:50:56: 8000000 INFO @ Tue, 16 Jun 2020 11:50:58: 1000000 INFO @ Tue, 16 Jun 2020 11:51:04: 5000000 INFO @ Tue, 16 Jun 2020 11:51:05: 9000000 INFO @ Tue, 16 Jun 2020 11:51:08: 2000000 INFO @ Tue, 16 Jun 2020 11:51:13: 6000000 INFO @ Tue, 16 Jun 2020 11:51:15: 10000000 INFO @ Tue, 16 Jun 2020 11:51:18: 3000000 INFO @ Tue, 16 Jun 2020 11:51:23: 7000000 INFO @ Tue, 16 Jun 2020 11:51:24: 11000000 INFO @ Tue, 16 Jun 2020 11:51:27: 4000000 INFO @ Tue, 16 Jun 2020 11:51:32: 8000000 INFO @ Tue, 16 Jun 2020 11:51:34: 12000000 INFO @ Tue, 16 Jun 2020 11:51:37: 5000000 INFO @ Tue, 16 Jun 2020 11:51:41: 9000000 INFO @ Tue, 16 Jun 2020 11:51:43: 13000000 INFO @ Tue, 16 Jun 2020 11:51:46: 6000000 INFO @ Tue, 16 Jun 2020 11:51:51: 10000000 INFO @ Tue, 16 Jun 2020 11:51:53: 14000000 INFO @ Tue, 16 Jun 2020 11:51:55: 7000000 INFO @ Tue, 16 Jun 2020 11:52:00: 11000000 INFO @ Tue, 16 Jun 2020 11:52:02: 15000000 INFO @ Tue, 16 Jun 2020 11:52:05: 8000000 INFO @ Tue, 16 Jun 2020 11:52:10: 12000000 INFO @ Tue, 16 Jun 2020 11:52:12: 16000000 INFO @ Tue, 16 Jun 2020 11:52:14: 9000000 INFO @ Tue, 16 Jun 2020 11:52:19: 13000000 INFO @ Tue, 16 Jun 2020 11:52:22: 17000000 INFO @ Tue, 16 Jun 2020 11:52:24: 10000000 INFO @ Tue, 16 Jun 2020 11:52:29: 14000000 INFO @ Tue, 16 Jun 2020 11:52:31: 18000000 INFO @ Tue, 16 Jun 2020 11:52:33: 11000000 INFO @ Tue, 16 Jun 2020 11:52:38: 15000000 INFO @ Tue, 16 Jun 2020 11:52:41: 19000000 INFO @ Tue, 16 Jun 2020 11:52:42: 12000000 INFO @ Tue, 16 Jun 2020 11:52:48: 16000000 INFO @ Tue, 16 Jun 2020 11:52:51: 20000000 INFO @ Tue, 16 Jun 2020 11:52:52: 13000000 INFO @ Tue, 16 Jun 2020 11:52:57: 17000000 INFO @ Tue, 16 Jun 2020 11:53:01: 21000000 INFO @ Tue, 16 Jun 2020 11:53:01: 14000000 INFO @ Tue, 16 Jun 2020 11:53:06: 18000000 INFO @ Tue, 16 Jun 2020 11:53:10: 22000000 INFO @ Tue, 16 Jun 2020 11:53:11: 15000000 INFO @ Tue, 16 Jun 2020 11:53:16: 19000000 INFO @ Tue, 16 Jun 2020 11:53:20: 16000000 INFO @ Tue, 16 Jun 2020 11:53:20: 23000000 INFO @ Tue, 16 Jun 2020 11:53:25: 20000000 INFO @ Tue, 16 Jun 2020 11:53:29: 17000000 INFO @ Tue, 16 Jun 2020 11:53:30: 24000000 INFO @ Tue, 16 Jun 2020 11:53:34: 21000000 INFO @ Tue, 16 Jun 2020 11:53:39: 18000000 INFO @ Tue, 16 Jun 2020 11:53:40: 25000000 INFO @ Tue, 16 Jun 2020 11:53:44: 22000000 INFO @ Tue, 16 Jun 2020 11:53:48: 19000000 INFO @ Tue, 16 Jun 2020 11:53:50: 26000000 INFO @ Tue, 16 Jun 2020 11:53:53: 23000000 INFO @ Tue, 16 Jun 2020 11:53:58: 20000000 INFO @ Tue, 16 Jun 2020 11:54:00: 27000000 INFO @ Tue, 16 Jun 2020 11:54:02: 24000000 INFO @ Tue, 16 Jun 2020 11:54:07: 21000000 INFO @ Tue, 16 Jun 2020 11:54:10: 28000000 INFO @ Tue, 16 Jun 2020 11:54:12: 25000000 INFO @ Tue, 16 Jun 2020 11:54:17: 22000000 INFO @ Tue, 16 Jun 2020 11:54:19: 29000000 INFO @ Tue, 16 Jun 2020 11:54:21: 26000000 INFO @ Tue, 16 Jun 2020 11:54:26: 23000000 INFO @ Tue, 16 Jun 2020 11:54:29: 30000000 INFO @ Tue, 16 Jun 2020 11:54:30: 27000000 INFO @ Tue, 16 Jun 2020 11:54:36: 24000000 INFO @ Tue, 16 Jun 2020 11:54:39: 31000000 INFO @ Tue, 16 Jun 2020 11:54:40: 28000000 INFO @ Tue, 16 Jun 2020 11:54:45: 25000000 INFO @ Tue, 16 Jun 2020 11:54:49: 32000000 INFO @ Tue, 16 Jun 2020 11:54:49: 29000000 INFO @ Tue, 16 Jun 2020 11:54:55: 26000000 INFO @ Tue, 16 Jun 2020 11:54:58: 33000000 INFO @ Tue, 16 Jun 2020 11:54:58: 30000000 INFO @ Tue, 16 Jun 2020 11:55:05: 27000000 INFO @ Tue, 16 Jun 2020 11:55:07: 31000000 INFO @ Tue, 16 Jun 2020 11:55:08: 34000000 INFO @ Tue, 16 Jun 2020 11:55:14: 28000000 INFO @ Tue, 16 Jun 2020 11:55:17: 32000000 INFO @ Tue, 16 Jun 2020 11:55:17: 35000000 INFO @ Tue, 16 Jun 2020 11:55:24: 29000000 INFO @ Tue, 16 Jun 2020 11:55:27: 36000000 INFO @ Tue, 16 Jun 2020 11:55:28: 33000000 INFO @ Tue, 16 Jun 2020 11:55:34: 30000000 INFO @ Tue, 16 Jun 2020 11:55:38: 37000000 INFO @ Tue, 16 Jun 2020 11:55:38: 34000000 INFO @ Tue, 16 Jun 2020 11:55:44: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 11:55:49: 35000000 INFO @ Tue, 16 Jun 2020 11:55:49: 38000000 INFO @ Tue, 16 Jun 2020 11:55:54: 32000000 INFO @ Tue, 16 Jun 2020 11:55:59: 36000000 INFO @ Tue, 16 Jun 2020 11:56:00: 39000000 INFO @ Tue, 16 Jun 2020 11:56:03: 33000000 INFO @ Tue, 16 Jun 2020 11:56:09: 37000000 INFO @ Tue, 16 Jun 2020 11:56:11: 40000000 INFO @ Tue, 16 Jun 2020 11:56:14: 34000000 INFO @ Tue, 16 Jun 2020 11:56:19: 38000000 INFO @ Tue, 16 Jun 2020 11:56:22: 41000000 INFO @ Tue, 16 Jun 2020 11:56:24: 35000000 INFO @ Tue, 16 Jun 2020 11:56:30: 39000000 INFO @ Tue, 16 Jun 2020 11:56:34: 42000000 INFO @ Tue, 16 Jun 2020 11:56:34: 36000000 INFO @ Tue, 16 Jun 2020 11:56:40: 40000000 INFO @ Tue, 16 Jun 2020 11:56:44: 37000000 INFO @ Tue, 16 Jun 2020 11:56:45: 43000000 INFO @ Tue, 16 Jun 2020 11:56:50: 41000000 INFO @ Tue, 16 Jun 2020 11:56:54: 38000000 INFO @ Tue, 16 Jun 2020 11:56:58: 44000000 INFO @ Tue, 16 Jun 2020 11:57:00: 42000000 INFO @ Tue, 16 Jun 2020 11:57:04: 39000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 11:57:10: 45000000 INFO @ Tue, 16 Jun 2020 11:57:11: 43000000 INFO @ Tue, 16 Jun 2020 11:57:14: 40000000 INFO @ Tue, 16 Jun 2020 11:57:21: 46000000 INFO @ Tue, 16 Jun 2020 11:57:22: 44000000 INFO @ Tue, 16 Jun 2020 11:57:24: 41000000 INFO @ Tue, 16 Jun 2020 11:57:32: 45000000 INFO @ Tue, 16 Jun 2020 11:57:32: 47000000 INFO @ Tue, 16 Jun 2020 11:57:33: 42000000 INFO @ Tue, 16 Jun 2020 11:57:42: 46000000 INFO @ Tue, 16 Jun 2020 11:57:43: 48000000 INFO @ Tue, 16 Jun 2020 11:57:43: 43000000 INFO @ Tue, 16 Jun 2020 11:57:53: 47000000 INFO @ Tue, 16 Jun 2020 11:57:54: 44000000 INFO @ Tue, 16 Jun 2020 11:57:54: 49000000 INFO @ Tue, 16 Jun 2020 11:58:03: 48000000 INFO @ Tue, 16 Jun 2020 11:58:04: 45000000 INFO @ Tue, 16 Jun 2020 11:58:06: 50000000 INFO @ Tue, 16 Jun 2020 11:58:13: 49000000 INFO @ Tue, 16 Jun 2020 11:58:14: 46000000 INFO @ Tue, 16 Jun 2020 11:58:17: 51000000 INFO @ Tue, 16 Jun 2020 11:58:23: 50000000 INFO @ Tue, 16 Jun 2020 11:58:23: 47000000 INFO @ Tue, 16 Jun 2020 11:58:27: 52000000 INFO @ Tue, 16 Jun 2020 11:58:33: 51000000 INFO @ Tue, 16 Jun 2020 11:58:33: 48000000 INFO @ Tue, 16 Jun 2020 11:58:39: 53000000 INFO @ Tue, 16 Jun 2020 11:58:43: 49000000 INFO @ Tue, 16 Jun 2020 11:58:43: 52000000 INFO @ Tue, 16 Jun 2020 11:58:47: #1 tag size is determined as 150 bps INFO @ Tue, 16 Jun 2020 11:58:47: #1 tag size = 150 INFO @ Tue, 16 Jun 2020 11:58:47: #1 total tags in treatment: 17433560 INFO @ Tue, 16 Jun 2020 11:58:47: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 11:58:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 11:58:47: #1 tags after filtering in treatment: 14375628 INFO @ Tue, 16 Jun 2020 11:58:47: #1 Redundant rate of treatment: 0.18 INFO @ Tue, 16 Jun 2020 11:58:47: #1 finished! INFO @ Tue, 16 Jun 2020 11:58:47: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 11:58:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 11:58:48: #2 number of paired peaks: 200 WARNING @ Tue, 16 Jun 2020 11:58:48: Fewer paired peaks (200) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 200 pairs to build model! INFO @ Tue, 16 Jun 2020 11:58:48: start model_add_line... INFO @ Tue, 16 Jun 2020 11:58:48: start X-correlation... INFO @ Tue, 16 Jun 2020 11:58:48: end of X-cor INFO @ Tue, 16 Jun 2020 11:58:48: #2 finished! INFO @ Tue, 16 Jun 2020 11:58:48: #2 predicted fragment length is 200 bps INFO @ Tue, 16 Jun 2020 11:58:48: #2 alternative fragment length(s) may be 174,200 bps INFO @ Tue, 16 Jun 2020 11:58:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX6619532/SRX6619532.05_model.r WARNING @ Tue, 16 Jun 2020 11:58:48: #2 Since the d (200) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 11:58:48: #2 You may need to consider one of the other alternative d(s): 174,200 WARNING @ Tue, 16 Jun 2020 11:58:48: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 11:58:48: #3 Call peaks... INFO @ Tue, 16 Jun 2020 11:58:48: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 11:58:53: 50000000 INFO @ Tue, 16 Jun 2020 11:58:53: 53000000 INFO @ Tue, 16 Jun 2020 11:59:01: #1 tag size is determined as 150 bps INFO @ Tue, 16 Jun 2020 11:59:01: #1 tag size = 150 INFO @ Tue, 16 Jun 2020 11:59:01: #1 total tags in treatment: 17433560 INFO @ Tue, 16 Jun 2020 11:59:01: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 11:59:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 11:59:01: #1 tags after filtering in treatment: 14375628 INFO @ Tue, 16 Jun 2020 11:59:01: #1 Redundant rate of treatment: 0.18 INFO @ Tue, 16 Jun 2020 11:59:01: #1 finished! INFO @ Tue, 16 Jun 2020 11:59:01: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 11:59:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 11:59:02: #2 number of paired peaks: 200 WARNING @ Tue, 16 Jun 2020 11:59:02: Fewer paired peaks (200) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 200 pairs to build model! INFO @ Tue, 16 Jun 2020 11:59:02: start model_add_line... INFO @ Tue, 16 Jun 2020 11:59:02: start X-correlation... INFO @ Tue, 16 Jun 2020 11:59:02: end of X-cor INFO @ Tue, 16 Jun 2020 11:59:02: #2 finished! INFO @ Tue, 16 Jun 2020 11:59:02: #2 predicted fragment length is 200 bps INFO @ Tue, 16 Jun 2020 11:59:02: #2 alternative fragment length(s) may be 174,200 bps INFO @ Tue, 16 Jun 2020 11:59:02: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX6619532/SRX6619532.10_model.r WARNING @ Tue, 16 Jun 2020 11:59:02: #2 Since the d (200) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 11:59:02: #2 You may need to consider one of the other alternative d(s): 174,200 WARNING @ Tue, 16 Jun 2020 11:59:02: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 11:59:02: #3 Call peaks... INFO @ Tue, 16 Jun 2020 11:59:02: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 11:59:02: 51000000 INFO @ Tue, 16 Jun 2020 11:59:11: 52000000 INFO @ Tue, 16 Jun 2020 11:59:21: 53000000 INFO @ Tue, 16 Jun 2020 11:59:21: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 11:59:27: #1 tag size is determined as 150 bps INFO @ Tue, 16 Jun 2020 11:59:27: #1 tag size = 150 INFO @ Tue, 16 Jun 2020 11:59:27: #1 total tags in treatment: 17433560 INFO @ Tue, 16 Jun 2020 11:59:27: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 11:59:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 11:59:28: #1 tags after filtering in treatment: 14375628 INFO @ Tue, 16 Jun 2020 11:59:28: #1 Redundant rate of treatment: 0.18 INFO @ Tue, 16 Jun 2020 11:59:28: #1 finished! INFO @ Tue, 16 Jun 2020 11:59:28: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 11:59:28: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 11:59:29: #2 number of paired peaks: 200 WARNING @ Tue, 16 Jun 2020 11:59:29: Fewer paired peaks (200) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 200 pairs to build model! INFO @ Tue, 16 Jun 2020 11:59:29: start model_add_line... INFO @ Tue, 16 Jun 2020 11:59:29: start X-correlation... INFO @ Tue, 16 Jun 2020 11:59:29: end of X-cor INFO @ Tue, 16 Jun 2020 11:59:29: #2 finished! INFO @ Tue, 16 Jun 2020 11:59:29: #2 predicted fragment length is 200 bps INFO @ Tue, 16 Jun 2020 11:59:29: #2 alternative fragment length(s) may be 174,200 bps INFO @ Tue, 16 Jun 2020 11:59:29: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX6619532/SRX6619532.20_model.r WARNING @ Tue, 16 Jun 2020 11:59:29: #2 Since the d (200) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 11:59:29: #2 You may need to consider one of the other alternative d(s): 174,200 WARNING @ Tue, 16 Jun 2020 11:59:29: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 11:59:29: #3 Call peaks... INFO @ Tue, 16 Jun 2020 11:59:29: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 11:59:35: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 11:59:37: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX6619532/SRX6619532.05_peaks.xls INFO @ Tue, 16 Jun 2020 11:59:37: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX6619532/SRX6619532.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 11:59:37: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX6619532/SRX6619532.05_summits.bed INFO @ Tue, 16 Jun 2020 11:59:37: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (444 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 11:59:51: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX6619532/SRX6619532.10_peaks.xls INFO @ Tue, 16 Jun 2020 11:59:51: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX6619532/SRX6619532.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 11:59:51: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX6619532/SRX6619532.10_summits.bed INFO @ Tue, 16 Jun 2020 11:59:51: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (319 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 12:00:01: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 12:00:16: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX6619532/SRX6619532.20_peaks.xls INFO @ Tue, 16 Jun 2020 12:00:16: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX6619532/SRX6619532.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 12:00:16: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX6619532/SRX6619532.20_summits.bed INFO @ Tue, 16 Jun 2020 12:00:16: Done! pass1 - making usageList (6 chroms): 2 millis pass2 - checking and writing primary data (209 records, 4 fields): 2 millis CompletedMACS2peakCalling