Job ID = 6368819 SRX = SRX5985673 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:36:20 prefetch.2.10.7: 1) Downloading 'SRR9214978'... 2020-06-16T00:36:20 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:40:30 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:40:30 prefetch.2.10.7: 1) 'SRR9214978' was downloaded successfully Read 40114650 spots for SRR9214978/SRR9214978.sra Written 40114650 spots for SRR9214978/SRR9214978.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:13:27 40114650 reads; of these: 40114650 (100.00%) were unpaired; of these: 330345 (0.82%) aligned 0 times 33389849 (83.24%) aligned exactly 1 time 6394456 (15.94%) aligned >1 times 99.18% overall alignment rate Time searching: 00:13:28 Overall time: 00:13:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 5536171 / 39784305 = 0.1392 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:06:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5985673/SRX5985673.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5985673/SRX5985673.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5985673/SRX5985673.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5985673/SRX5985673.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:06:57: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:06:57: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:07:03: 1000000 INFO @ Tue, 16 Jun 2020 10:07:09: 2000000 INFO @ Tue, 16 Jun 2020 10:07:15: 3000000 INFO @ Tue, 16 Jun 2020 10:07:21: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:07:27: 5000000 INFO @ Tue, 16 Jun 2020 10:07:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5985673/SRX5985673.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5985673/SRX5985673.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5985673/SRX5985673.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5985673/SRX5985673.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:07:27: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:07:27: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:07:33: 6000000 INFO @ Tue, 16 Jun 2020 10:07:33: 1000000 INFO @ Tue, 16 Jun 2020 10:07:39: 7000000 INFO @ Tue, 16 Jun 2020 10:07:40: 2000000 INFO @ Tue, 16 Jun 2020 10:07:45: 8000000 INFO @ Tue, 16 Jun 2020 10:07:46: 3000000 INFO @ Tue, 16 Jun 2020 10:07:51: 9000000 INFO @ Tue, 16 Jun 2020 10:07:52: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:07:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5985673/SRX5985673.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5985673/SRX5985673.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5985673/SRX5985673.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5985673/SRX5985673.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:07:57: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:07:57: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:07:57: 10000000 INFO @ Tue, 16 Jun 2020 10:07:58: 5000000 INFO @ Tue, 16 Jun 2020 10:08:04: 11000000 INFO @ Tue, 16 Jun 2020 10:08:04: 1000000 INFO @ Tue, 16 Jun 2020 10:08:05: 6000000 INFO @ Tue, 16 Jun 2020 10:08:10: 12000000 INFO @ Tue, 16 Jun 2020 10:08:10: 2000000 INFO @ Tue, 16 Jun 2020 10:08:11: 7000000 INFO @ Tue, 16 Jun 2020 10:08:16: 13000000 INFO @ Tue, 16 Jun 2020 10:08:17: 3000000 INFO @ Tue, 16 Jun 2020 10:08:17: 8000000 INFO @ Tue, 16 Jun 2020 10:08:22: 14000000 INFO @ Tue, 16 Jun 2020 10:08:23: 4000000 INFO @ Tue, 16 Jun 2020 10:08:24: 9000000 INFO @ Tue, 16 Jun 2020 10:08:28: 15000000 INFO @ Tue, 16 Jun 2020 10:08:30: 5000000 INFO @ Tue, 16 Jun 2020 10:08:30: 10000000 INFO @ Tue, 16 Jun 2020 10:08:34: 16000000 INFO @ Tue, 16 Jun 2020 10:08:36: 6000000 INFO @ Tue, 16 Jun 2020 10:08:36: 11000000 INFO @ Tue, 16 Jun 2020 10:08:41: 17000000 INFO @ Tue, 16 Jun 2020 10:08:42: 7000000 INFO @ Tue, 16 Jun 2020 10:08:42: 12000000 INFO @ Tue, 16 Jun 2020 10:08:47: 18000000 INFO @ Tue, 16 Jun 2020 10:08:48: 8000000 INFO @ Tue, 16 Jun 2020 10:08:49: 13000000 INFO @ Tue, 16 Jun 2020 10:08:54: 19000000 INFO @ Tue, 16 Jun 2020 10:08:55: 9000000 INFO @ Tue, 16 Jun 2020 10:08:55: 14000000 INFO @ Tue, 16 Jun 2020 10:09:00: 20000000 INFO @ Tue, 16 Jun 2020 10:09:01: 15000000 INFO @ Tue, 16 Jun 2020 10:09:01: 10000000 INFO @ Tue, 16 Jun 2020 10:09:06: 21000000 INFO @ Tue, 16 Jun 2020 10:09:07: 16000000 INFO @ Tue, 16 Jun 2020 10:09:07: 11000000 INFO @ Tue, 16 Jun 2020 10:09:13: 22000000 INFO @ Tue, 16 Jun 2020 10:09:14: 12000000 INFO @ Tue, 16 Jun 2020 10:09:14: 17000000 INFO @ Tue, 16 Jun 2020 10:09:19: 23000000 INFO @ Tue, 16 Jun 2020 10:09:20: 13000000 INFO @ Tue, 16 Jun 2020 10:09:21: 18000000 INFO @ Tue, 16 Jun 2020 10:09:26: 24000000 INFO @ Tue, 16 Jun 2020 10:09:27: 14000000 INFO @ Tue, 16 Jun 2020 10:09:27: 19000000 INFO @ Tue, 16 Jun 2020 10:09:33: 15000000 INFO @ Tue, 16 Jun 2020 10:09:33: 25000000 INFO @ Tue, 16 Jun 2020 10:09:33: 20000000 INFO @ Tue, 16 Jun 2020 10:09:39: 16000000 INFO @ Tue, 16 Jun 2020 10:09:39: 26000000 INFO @ Tue, 16 Jun 2020 10:09:40: 21000000 INFO @ Tue, 16 Jun 2020 10:09:46: 22000000 INFO @ Tue, 16 Jun 2020 10:09:46: 17000000 INFO @ Tue, 16 Jun 2020 10:09:46: 27000000 INFO @ Tue, 16 Jun 2020 10:09:52: 23000000 INFO @ Tue, 16 Jun 2020 10:09:53: 28000000 INFO @ Tue, 16 Jun 2020 10:09:53: 18000000 INFO @ Tue, 16 Jun 2020 10:09:59: 29000000 INFO @ Tue, 16 Jun 2020 10:09:59: 24000000 INFO @ Tue, 16 Jun 2020 10:10:00: 19000000 INFO @ Tue, 16 Jun 2020 10:10:05: 30000000 INFO @ Tue, 16 Jun 2020 10:10:06: 25000000 INFO @ Tue, 16 Jun 2020 10:10:06: 20000000 INFO @ Tue, 16 Jun 2020 10:10:12: 31000000 INFO @ Tue, 16 Jun 2020 10:10:12: 26000000 INFO @ Tue, 16 Jun 2020 10:10:13: 21000000 INFO @ Tue, 16 Jun 2020 10:10:18: 32000000 INFO @ Tue, 16 Jun 2020 10:10:19: 27000000 INFO @ Tue, 16 Jun 2020 10:10:19: 22000000 INFO @ Tue, 16 Jun 2020 10:10:25: 33000000 INFO @ Tue, 16 Jun 2020 10:10:26: 28000000 INFO @ Tue, 16 Jun 2020 10:10:26: 23000000 INFO @ Tue, 16 Jun 2020 10:10:32: 34000000 INFO @ Tue, 16 Jun 2020 10:10:33: 29000000 INFO @ Tue, 16 Jun 2020 10:10:33: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:10:33: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:10:33: #1 total tags in treatment: 34248134 INFO @ Tue, 16 Jun 2020 10:10:33: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:10:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:10:34: 24000000 INFO @ Tue, 16 Jun 2020 10:10:34: #1 tags after filtering in treatment: 34248134 INFO @ Tue, 16 Jun 2020 10:10:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:10:34: #1 finished! INFO @ Tue, 16 Jun 2020 10:10:34: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:10:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:10:36: #2 number of paired peaks: 40 WARNING @ Tue, 16 Jun 2020 10:10:36: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:10:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5985673/SRX5985673.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5985673/SRX5985673.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5985673/SRX5985673.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5985673/SRX5985673.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:10:39: 30000000 INFO @ Tue, 16 Jun 2020 10:10:41: 25000000 INFO @ Tue, 16 Jun 2020 10:10:45: 31000000 INFO @ Tue, 16 Jun 2020 10:10:47: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:10:52: 32000000 INFO @ Tue, 16 Jun 2020 10:10:54: 27000000 INFO @ Tue, 16 Jun 2020 10:10:58: 33000000 INFO @ Tue, 16 Jun 2020 10:11:00: 28000000 INFO @ Tue, 16 Jun 2020 10:11:05: 34000000 INFO @ Tue, 16 Jun 2020 10:11:07: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:11:07: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:11:07: #1 total tags in treatment: 34248134 INFO @ Tue, 16 Jun 2020 10:11:07: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:11:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:11:07: 29000000 INFO @ Tue, 16 Jun 2020 10:11:07: #1 tags after filtering in treatment: 34248134 INFO @ Tue, 16 Jun 2020 10:11:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:11:07: #1 finished! INFO @ Tue, 16 Jun 2020 10:11:07: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:11:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:11:10: #2 number of paired peaks: 40 WARNING @ Tue, 16 Jun 2020 10:11:10: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:11:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5985673/SRX5985673.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5985673/SRX5985673.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5985673/SRX5985673.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5985673/SRX5985673.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:11:13: 30000000 INFO @ Tue, 16 Jun 2020 10:11:19: 31000000 INFO @ Tue, 16 Jun 2020 10:11:25: 32000000 INFO @ Tue, 16 Jun 2020 10:11:32: 33000000 INFO @ Tue, 16 Jun 2020 10:11:38: 34000000 INFO @ Tue, 16 Jun 2020 10:11:40: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:11:40: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:11:40: #1 total tags in treatment: 34248134 INFO @ Tue, 16 Jun 2020 10:11:40: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:11:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:11:41: #1 tags after filtering in treatment: 34248134 INFO @ Tue, 16 Jun 2020 10:11:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:11:41: #1 finished! INFO @ Tue, 16 Jun 2020 10:11:41: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:11:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:11:43: #2 number of paired peaks: 40 WARNING @ Tue, 16 Jun 2020 10:11:43: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:11:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5985673/SRX5985673.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5985673/SRX5985673.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5985673/SRX5985673.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5985673/SRX5985673.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。