Job ID = 6368790 SRX = SRX5702597 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:28:49 prefetch.2.10.7: 1) Downloading 'SRR8921274'... 2020-06-16T00:28:49 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:29:53 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:29:54 prefetch.2.10.7: 'SRR8921274' is valid 2020-06-16T00:29:54 prefetch.2.10.7: 1) 'SRR8921274' was downloaded successfully 2020-06-16T00:29:54 prefetch.2.10.7: 'SRR8921274' has 0 unresolved dependencies Read 18940672 spots for SRR8921274/SRR8921274.sra Written 18940672 spots for SRR8921274/SRR8921274.sra 2020-06-16T00:31:17 prefetch.2.10.7: 1) Downloading 'SRR8921275'... 2020-06-16T00:31:17 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:35:40 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:35:40 prefetch.2.10.7: 1) 'SRR8921275' was downloaded successfully 2020-06-16T00:35:40 prefetch.2.10.7: 'SRR8921275' has 0 unresolved dependencies Read 50398183 spots for SRR8921275/SRR8921275.sra Written 50398183 spots for SRR8921275/SRR8921275.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:47 69338855 reads; of these: 69338855 (100.00%) were unpaired; of these: 24275992 (35.01%) aligned 0 times 36850738 (53.15%) aligned exactly 1 time 8212125 (11.84%) aligned >1 times 64.99% overall alignment rate Time searching: 00:17:47 Overall time: 00:17:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 21595394 / 45062863 = 0.4792 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:10:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5702597/SRX5702597.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5702597/SRX5702597.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5702597/SRX5702597.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5702597/SRX5702597.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:10:24: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:10:24: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:10:32: 1000000 INFO @ Tue, 16 Jun 2020 10:10:40: 2000000 INFO @ Tue, 16 Jun 2020 10:10:48: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:10:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5702597/SRX5702597.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5702597/SRX5702597.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5702597/SRX5702597.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5702597/SRX5702597.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:10:54: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:10:54: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:10:55: 4000000 INFO @ Tue, 16 Jun 2020 10:11:01: 1000000 INFO @ Tue, 16 Jun 2020 10:11:03: 5000000 INFO @ Tue, 16 Jun 2020 10:11:09: 2000000 INFO @ Tue, 16 Jun 2020 10:11:11: 6000000 INFO @ Tue, 16 Jun 2020 10:11:17: 3000000 INFO @ Tue, 16 Jun 2020 10:11:18: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:11:25: 4000000 INFO @ Tue, 16 Jun 2020 10:11:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5702597/SRX5702597.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5702597/SRX5702597.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5702597/SRX5702597.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5702597/SRX5702597.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:11:25: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:11:25: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:11:26: 8000000 INFO @ Tue, 16 Jun 2020 10:11:34: 5000000 INFO @ Tue, 16 Jun 2020 10:11:34: 9000000 INFO @ Tue, 16 Jun 2020 10:11:35: 1000000 INFO @ Tue, 16 Jun 2020 10:11:42: 10000000 INFO @ Tue, 16 Jun 2020 10:11:42: 6000000 INFO @ Tue, 16 Jun 2020 10:11:45: 2000000 INFO @ Tue, 16 Jun 2020 10:11:50: 11000000 INFO @ Tue, 16 Jun 2020 10:11:51: 7000000 INFO @ Tue, 16 Jun 2020 10:11:54: 3000000 INFO @ Tue, 16 Jun 2020 10:11:58: 12000000 INFO @ Tue, 16 Jun 2020 10:12:01: 8000000 INFO @ Tue, 16 Jun 2020 10:12:02: 4000000 INFO @ Tue, 16 Jun 2020 10:12:06: 13000000 INFO @ Tue, 16 Jun 2020 10:12:09: 9000000 INFO @ Tue, 16 Jun 2020 10:12:11: 5000000 INFO @ Tue, 16 Jun 2020 10:12:14: 14000000 INFO @ Tue, 16 Jun 2020 10:12:17: 10000000 INFO @ Tue, 16 Jun 2020 10:12:20: 6000000 INFO @ Tue, 16 Jun 2020 10:12:22: 15000000 INFO @ Tue, 16 Jun 2020 10:12:25: 11000000 INFO @ Tue, 16 Jun 2020 10:12:28: 7000000 INFO @ Tue, 16 Jun 2020 10:12:29: 16000000 INFO @ Tue, 16 Jun 2020 10:12:33: 12000000 INFO @ Tue, 16 Jun 2020 10:12:36: 8000000 INFO @ Tue, 16 Jun 2020 10:12:37: 17000000 INFO @ Tue, 16 Jun 2020 10:12:40: 13000000 INFO @ Tue, 16 Jun 2020 10:12:43: 9000000 INFO @ Tue, 16 Jun 2020 10:12:44: 18000000 INFO @ Tue, 16 Jun 2020 10:12:47: 14000000 INFO @ Tue, 16 Jun 2020 10:12:50: 10000000 INFO @ Tue, 16 Jun 2020 10:12:52: 19000000 INFO @ Tue, 16 Jun 2020 10:12:54: 15000000 INFO @ Tue, 16 Jun 2020 10:12:57: 11000000 INFO @ Tue, 16 Jun 2020 10:12:59: 20000000 INFO @ Tue, 16 Jun 2020 10:13:01: 16000000 INFO @ Tue, 16 Jun 2020 10:13:04: 12000000 INFO @ Tue, 16 Jun 2020 10:13:07: 21000000 INFO @ Tue, 16 Jun 2020 10:13:08: 17000000 INFO @ Tue, 16 Jun 2020 10:13:11: 13000000 INFO @ Tue, 16 Jun 2020 10:13:15: 22000000 INFO @ Tue, 16 Jun 2020 10:13:15: 18000000 INFO @ Tue, 16 Jun 2020 10:13:18: 14000000 INFO @ Tue, 16 Jun 2020 10:13:22: 19000000 INFO @ Tue, 16 Jun 2020 10:13:22: 23000000 INFO @ Tue, 16 Jun 2020 10:13:25: 15000000 INFO @ Tue, 16 Jun 2020 10:13:26: #1 tag size is determined as 55 bps INFO @ Tue, 16 Jun 2020 10:13:26: #1 tag size = 55 INFO @ Tue, 16 Jun 2020 10:13:26: #1 total tags in treatment: 23467469 INFO @ Tue, 16 Jun 2020 10:13:26: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:13:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:13:26: #1 tags after filtering in treatment: 23467469 INFO @ Tue, 16 Jun 2020 10:13:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:13:26: #1 finished! INFO @ Tue, 16 Jun 2020 10:13:26: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:13:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:13:28: #2 number of paired peaks: 96 WARNING @ Tue, 16 Jun 2020 10:13:28: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:13:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5702597/SRX5702597.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5702597/SRX5702597.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5702597/SRX5702597.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5702597/SRX5702597.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:13:29: 20000000 INFO @ Tue, 16 Jun 2020 10:13:32: 16000000 INFO @ Tue, 16 Jun 2020 10:13:36: 21000000 INFO @ Tue, 16 Jun 2020 10:13:38: 17000000 INFO @ Tue, 16 Jun 2020 10:13:42: 22000000 INFO @ Tue, 16 Jun 2020 10:13:45: 18000000 INFO @ Tue, 16 Jun 2020 10:13:49: 23000000 INFO @ Tue, 16 Jun 2020 10:13:51: 19000000 INFO @ Tue, 16 Jun 2020 10:13:52: #1 tag size is determined as 55 bps INFO @ Tue, 16 Jun 2020 10:13:52: #1 tag size = 55 INFO @ Tue, 16 Jun 2020 10:13:52: #1 total tags in treatment: 23467469 INFO @ Tue, 16 Jun 2020 10:13:52: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:13:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:13:52: #1 tags after filtering in treatment: 23467469 INFO @ Tue, 16 Jun 2020 10:13:52: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:13:52: #1 finished! INFO @ Tue, 16 Jun 2020 10:13:52: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:13:52: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:13:54: #2 number of paired peaks: 96 WARNING @ Tue, 16 Jun 2020 10:13:54: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:13:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5702597/SRX5702597.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5702597/SRX5702597.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5702597/SRX5702597.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5702597/SRX5702597.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:13:57: 20000000 INFO @ Tue, 16 Jun 2020 10:14:03: 21000000 INFO @ Tue, 16 Jun 2020 10:14:08: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:14:14: 23000000 INFO @ Tue, 16 Jun 2020 10:14:17: #1 tag size is determined as 55 bps INFO @ Tue, 16 Jun 2020 10:14:17: #1 tag size = 55 INFO @ Tue, 16 Jun 2020 10:14:17: #1 total tags in treatment: 23467469 INFO @ Tue, 16 Jun 2020 10:14:17: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:14:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:14:18: #1 tags after filtering in treatment: 23467469 INFO @ Tue, 16 Jun 2020 10:14:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:14:18: #1 finished! INFO @ Tue, 16 Jun 2020 10:14:18: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:14:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:14:19: #2 number of paired peaks: 96 WARNING @ Tue, 16 Jun 2020 10:14:19: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:14:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5702597/SRX5702597.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5702597/SRX5702597.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5702597/SRX5702597.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5702597/SRX5702597.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。