Job ID = 6368787 SRX = SRX5702594 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:22:24 prefetch.2.10.7: 1) Downloading 'SRR8921270'... 2020-06-16T00:22:24 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:23:29 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:23:30 prefetch.2.10.7: 'SRR8921270' is valid 2020-06-16T00:23:30 prefetch.2.10.7: 1) 'SRR8921270' was downloaded successfully 2020-06-16T00:23:30 prefetch.2.10.7: 'SRR8921270' has 0 unresolved dependencies Read 23723506 spots for SRR8921270/SRR8921270.sra Written 23723506 spots for SRR8921270/SRR8921270.sra 2020-06-16T00:25:11 prefetch.2.10.7: 1) Downloading 'SRR8921271'... 2020-06-16T00:25:11 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:26:47 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:26:47 prefetch.2.10.7: 'SRR8921271' is valid 2020-06-16T00:26:47 prefetch.2.10.7: 1) 'SRR8921271' was downloaded successfully 2020-06-16T00:26:47 prefetch.2.10.7: 'SRR8921271' has 0 unresolved dependencies Read 20111351 spots for SRR8921271/SRR8921271.sra Written 20111351 spots for SRR8921271/SRR8921271.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:49 43834857 reads; of these: 43834857 (100.00%) were unpaired; of these: 806953 (1.84%) aligned 0 times 36090943 (82.33%) aligned exactly 1 time 6936961 (15.83%) aligned >1 times 98.16% overall alignment rate Time searching: 00:09:49 Overall time: 00:09:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 12086492 / 43027904 = 0.2809 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:47:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5702594/SRX5702594.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5702594/SRX5702594.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5702594/SRX5702594.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5702594/SRX5702594.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:47:26: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:47:26: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:47:31: 1000000 INFO @ Tue, 16 Jun 2020 09:47:37: 2000000 INFO @ Tue, 16 Jun 2020 09:47:43: 3000000 INFO @ Tue, 16 Jun 2020 09:47:48: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:47:54: 5000000 INFO @ Tue, 16 Jun 2020 09:47:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5702594/SRX5702594.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5702594/SRX5702594.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5702594/SRX5702594.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5702594/SRX5702594.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:47:56: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:47:56: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:48:00: 6000000 INFO @ Tue, 16 Jun 2020 09:48:02: 1000000 INFO @ Tue, 16 Jun 2020 09:48:06: 7000000 INFO @ Tue, 16 Jun 2020 09:48:08: 2000000 INFO @ Tue, 16 Jun 2020 09:48:12: 8000000 INFO @ Tue, 16 Jun 2020 09:48:15: 3000000 INFO @ Tue, 16 Jun 2020 09:48:18: 9000000 INFO @ Tue, 16 Jun 2020 09:48:21: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:48:24: 10000000 INFO @ Tue, 16 Jun 2020 09:48:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5702594/SRX5702594.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5702594/SRX5702594.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5702594/SRX5702594.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5702594/SRX5702594.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:48:26: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:48:26: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:48:27: 5000000 INFO @ Tue, 16 Jun 2020 09:48:31: 11000000 INFO @ Tue, 16 Jun 2020 09:48:33: 1000000 INFO @ Tue, 16 Jun 2020 09:48:34: 6000000 INFO @ Tue, 16 Jun 2020 09:48:37: 12000000 INFO @ Tue, 16 Jun 2020 09:48:39: 2000000 INFO @ Tue, 16 Jun 2020 09:48:40: 7000000 INFO @ Tue, 16 Jun 2020 09:48:43: 13000000 INFO @ Tue, 16 Jun 2020 09:48:46: 3000000 INFO @ Tue, 16 Jun 2020 09:48:46: 8000000 INFO @ Tue, 16 Jun 2020 09:48:50: 14000000 INFO @ Tue, 16 Jun 2020 09:48:52: 4000000 INFO @ Tue, 16 Jun 2020 09:48:53: 9000000 INFO @ Tue, 16 Jun 2020 09:48:56: 15000000 INFO @ Tue, 16 Jun 2020 09:48:59: 5000000 INFO @ Tue, 16 Jun 2020 09:48:59: 10000000 INFO @ Tue, 16 Jun 2020 09:49:03: 16000000 INFO @ Tue, 16 Jun 2020 09:49:05: 6000000 INFO @ Tue, 16 Jun 2020 09:49:06: 11000000 INFO @ Tue, 16 Jun 2020 09:49:09: 17000000 INFO @ Tue, 16 Jun 2020 09:49:12: 7000000 INFO @ Tue, 16 Jun 2020 09:49:12: 12000000 INFO @ Tue, 16 Jun 2020 09:49:15: 18000000 INFO @ Tue, 16 Jun 2020 09:49:18: 8000000 INFO @ Tue, 16 Jun 2020 09:49:19: 13000000 INFO @ Tue, 16 Jun 2020 09:49:22: 19000000 INFO @ Tue, 16 Jun 2020 09:49:25: 9000000 INFO @ Tue, 16 Jun 2020 09:49:25: 14000000 INFO @ Tue, 16 Jun 2020 09:49:28: 20000000 INFO @ Tue, 16 Jun 2020 09:49:31: 10000000 INFO @ Tue, 16 Jun 2020 09:49:32: 15000000 INFO @ Tue, 16 Jun 2020 09:49:35: 21000000 INFO @ Tue, 16 Jun 2020 09:49:38: 11000000 INFO @ Tue, 16 Jun 2020 09:49:38: 16000000 INFO @ Tue, 16 Jun 2020 09:49:41: 22000000 INFO @ Tue, 16 Jun 2020 09:49:44: 12000000 INFO @ Tue, 16 Jun 2020 09:49:44: 17000000 INFO @ Tue, 16 Jun 2020 09:49:48: 23000000 INFO @ Tue, 16 Jun 2020 09:49:51: 18000000 INFO @ Tue, 16 Jun 2020 09:49:51: 13000000 INFO @ Tue, 16 Jun 2020 09:49:54: 24000000 INFO @ Tue, 16 Jun 2020 09:49:57: 19000000 INFO @ Tue, 16 Jun 2020 09:49:58: 14000000 INFO @ Tue, 16 Jun 2020 09:50:00: 25000000 INFO @ Tue, 16 Jun 2020 09:50:04: 20000000 INFO @ Tue, 16 Jun 2020 09:50:04: 15000000 INFO @ Tue, 16 Jun 2020 09:50:07: 26000000 INFO @ Tue, 16 Jun 2020 09:50:10: 21000000 INFO @ Tue, 16 Jun 2020 09:50:11: 16000000 INFO @ Tue, 16 Jun 2020 09:50:13: 27000000 INFO @ Tue, 16 Jun 2020 09:50:17: 22000000 INFO @ Tue, 16 Jun 2020 09:50:17: 17000000 INFO @ Tue, 16 Jun 2020 09:50:20: 28000000 INFO @ Tue, 16 Jun 2020 09:50:23: 23000000 INFO @ Tue, 16 Jun 2020 09:50:24: 18000000 INFO @ Tue, 16 Jun 2020 09:50:26: 29000000 INFO @ Tue, 16 Jun 2020 09:50:29: 24000000 INFO @ Tue, 16 Jun 2020 09:50:30: 19000000 INFO @ Tue, 16 Jun 2020 09:50:32: 30000000 INFO @ Tue, 16 Jun 2020 09:50:36: 25000000 INFO @ Tue, 16 Jun 2020 09:50:37: 20000000 INFO @ Tue, 16 Jun 2020 09:50:38: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:50:38: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:50:38: #1 total tags in treatment: 30941412 INFO @ Tue, 16 Jun 2020 09:50:38: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:50:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:50:39: #1 tags after filtering in treatment: 30941412 INFO @ Tue, 16 Jun 2020 09:50:39: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:50:39: #1 finished! INFO @ Tue, 16 Jun 2020 09:50:39: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:50:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:50:40: #2 number of paired peaks: 46 WARNING @ Tue, 16 Jun 2020 09:50:40: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:50:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5702594/SRX5702594.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5702594/SRX5702594.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5702594/SRX5702594.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5702594/SRX5702594.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:50:42: 26000000 INFO @ Tue, 16 Jun 2020 09:50:43: 21000000 INFO @ Tue, 16 Jun 2020 09:50:48: 27000000 INFO @ Tue, 16 Jun 2020 09:50:50: 22000000 INFO @ Tue, 16 Jun 2020 09:50:53: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:50:56: 23000000 INFO @ Tue, 16 Jun 2020 09:50:59: 29000000 INFO @ Tue, 16 Jun 2020 09:51:02: 24000000 INFO @ Tue, 16 Jun 2020 09:51:05: 30000000 INFO @ Tue, 16 Jun 2020 09:51:09: 25000000 INFO @ Tue, 16 Jun 2020 09:51:11: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:51:11: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:51:11: #1 total tags in treatment: 30941412 INFO @ Tue, 16 Jun 2020 09:51:11: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:51:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:51:12: #1 tags after filtering in treatment: 30941412 INFO @ Tue, 16 Jun 2020 09:51:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:51:12: #1 finished! INFO @ Tue, 16 Jun 2020 09:51:12: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:51:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:51:14: #2 number of paired peaks: 46 WARNING @ Tue, 16 Jun 2020 09:51:14: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:51:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5702594/SRX5702594.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5702594/SRX5702594.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5702594/SRX5702594.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5702594/SRX5702594.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:51:15: 26000000 INFO @ Tue, 16 Jun 2020 09:51:21: 27000000 INFO @ Tue, 16 Jun 2020 09:51:27: 28000000 INFO @ Tue, 16 Jun 2020 09:51:33: 29000000 INFO @ Tue, 16 Jun 2020 09:51:39: 30000000 INFO @ Tue, 16 Jun 2020 09:51:45: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:51:45: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:51:45: #1 total tags in treatment: 30941412 INFO @ Tue, 16 Jun 2020 09:51:45: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:51:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:51:45: #1 tags after filtering in treatment: 30941412 INFO @ Tue, 16 Jun 2020 09:51:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:51:45: #1 finished! INFO @ Tue, 16 Jun 2020 09:51:45: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:51:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:51:47: #2 number of paired peaks: 46 WARNING @ Tue, 16 Jun 2020 09:51:47: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:51:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5702594/SRX5702594.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5702594/SRX5702594.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5702594/SRX5702594.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5702594/SRX5702594.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。