Job ID = 6368780 SRX = SRX5702588 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:36:04 prefetch.2.10.7: 1) Downloading 'SRR8921254'... 2020-06-16T00:36:04 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:39:22 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:39:22 prefetch.2.10.7: 1) 'SRR8921254' was downloaded successfully 2020-06-16T00:39:22 prefetch.2.10.7: 'SRR8921254' has 0 unresolved dependencies Read 37389429 spots for SRR8921254/SRR8921254.sra Written 37389429 spots for SRR8921254/SRR8921254.sra 2020-06-16T00:41:33 prefetch.2.10.7: 1) Downloading 'SRR8921255'... 2020-06-16T00:41:33 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:43:17 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:43:17 prefetch.2.10.7: 'SRR8921255' is valid 2020-06-16T00:43:17 prefetch.2.10.7: 1) 'SRR8921255' was downloaded successfully 2020-06-16T00:43:17 prefetch.2.10.7: 'SRR8921255' has 0 unresolved dependencies Read 22817177 spots for SRR8921255/SRR8921255.sra Written 22817177 spots for SRR8921255/SRR8921255.sra 2020-06-16T00:44:38 prefetch.2.10.7: 1) Downloading 'SRR8921256'... 2020-06-16T00:44:38 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:57:50 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:57:50 prefetch.2.10.7: 1) 'SRR8921256' was downloaded successfully 2020-06-16T00:57:50 prefetch.2.10.7: 'SRR8921256' has 0 unresolved dependencies Read 105354363 spots for SRR8921256/SRR8921256.sra Written 105354363 spots for SRR8921256/SRR8921256.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:37:43 165560969 reads; of these: 165560969 (100.00%) were unpaired; of these: 85616790 (51.71%) aligned 0 times 56337693 (34.03%) aligned exactly 1 time 23606486 (14.26%) aligned >1 times 48.29% overall alignment rate Time searching: 00:37:43 Overall time: 00:37:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 36 files... [bam_rmdupse_core] 36053158 / 79944179 = 0.4510 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 11:03:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5702588/SRX5702588.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5702588/SRX5702588.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5702588/SRX5702588.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5702588/SRX5702588.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 11:03:33: #1 read tag files... INFO @ Tue, 16 Jun 2020 11:03:33: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 11:03:38: 1000000 INFO @ Tue, 16 Jun 2020 11:03:43: 2000000 INFO @ Tue, 16 Jun 2020 11:03:48: 3000000 INFO @ Tue, 16 Jun 2020 11:03:54: 4000000 INFO @ Tue, 16 Jun 2020 11:03:59: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 11:04:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5702588/SRX5702588.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5702588/SRX5702588.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5702588/SRX5702588.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5702588/SRX5702588.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 11:04:03: #1 read tag files... INFO @ Tue, 16 Jun 2020 11:04:03: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 11:04:04: 6000000 INFO @ Tue, 16 Jun 2020 11:04:08: 1000000 INFO @ Tue, 16 Jun 2020 11:04:10: 7000000 INFO @ Tue, 16 Jun 2020 11:04:14: 2000000 INFO @ Tue, 16 Jun 2020 11:04:15: 8000000 INFO @ Tue, 16 Jun 2020 11:04:19: 3000000 INFO @ Tue, 16 Jun 2020 11:04:20: 9000000 INFO @ Tue, 16 Jun 2020 11:04:25: 4000000 INFO @ Tue, 16 Jun 2020 11:04:26: 10000000 INFO @ Tue, 16 Jun 2020 11:04:30: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 11:04:31: 11000000 INFO @ Tue, 16 Jun 2020 11:04:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5702588/SRX5702588.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5702588/SRX5702588.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5702588/SRX5702588.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5702588/SRX5702588.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 11:04:33: #1 read tag files... INFO @ Tue, 16 Jun 2020 11:04:33: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 11:04:36: 6000000 INFO @ Tue, 16 Jun 2020 11:04:37: 12000000 INFO @ Tue, 16 Jun 2020 11:04:39: 1000000 INFO @ Tue, 16 Jun 2020 11:04:42: 7000000 INFO @ Tue, 16 Jun 2020 11:04:43: 13000000 INFO @ Tue, 16 Jun 2020 11:04:45: 2000000 INFO @ Tue, 16 Jun 2020 11:04:48: 8000000 INFO @ Tue, 16 Jun 2020 11:04:48: 14000000 INFO @ Tue, 16 Jun 2020 11:04:51: 3000000 INFO @ Tue, 16 Jun 2020 11:04:54: 9000000 INFO @ Tue, 16 Jun 2020 11:04:54: 15000000 INFO @ Tue, 16 Jun 2020 11:04:57: 4000000 INFO @ Tue, 16 Jun 2020 11:04:59: 10000000 INFO @ Tue, 16 Jun 2020 11:04:59: 16000000 INFO @ Tue, 16 Jun 2020 11:05:03: 5000000 INFO @ Tue, 16 Jun 2020 11:05:05: 17000000 INFO @ Tue, 16 Jun 2020 11:05:05: 11000000 INFO @ Tue, 16 Jun 2020 11:05:08: 6000000 INFO @ Tue, 16 Jun 2020 11:05:11: 18000000 INFO @ Tue, 16 Jun 2020 11:05:11: 12000000 INFO @ Tue, 16 Jun 2020 11:05:14: 7000000 INFO @ Tue, 16 Jun 2020 11:05:16: 19000000 INFO @ Tue, 16 Jun 2020 11:05:16: 13000000 INFO @ Tue, 16 Jun 2020 11:05:20: 8000000 INFO @ Tue, 16 Jun 2020 11:05:22: 20000000 INFO @ Tue, 16 Jun 2020 11:05:22: 14000000 INFO @ Tue, 16 Jun 2020 11:05:25: 9000000 INFO @ Tue, 16 Jun 2020 11:05:27: 21000000 INFO @ Tue, 16 Jun 2020 11:05:28: 15000000 INFO @ Tue, 16 Jun 2020 11:05:31: 10000000 INFO @ Tue, 16 Jun 2020 11:05:33: 22000000 INFO @ Tue, 16 Jun 2020 11:05:33: 16000000 INFO @ Tue, 16 Jun 2020 11:05:37: 11000000 INFO @ Tue, 16 Jun 2020 11:05:38: 23000000 INFO @ Tue, 16 Jun 2020 11:05:39: 17000000 INFO @ Tue, 16 Jun 2020 11:05:42: 12000000 INFO @ Tue, 16 Jun 2020 11:05:44: 24000000 INFO @ Tue, 16 Jun 2020 11:05:44: 18000000 INFO @ Tue, 16 Jun 2020 11:05:48: 13000000 INFO @ Tue, 16 Jun 2020 11:05:50: 25000000 INFO @ Tue, 16 Jun 2020 11:05:50: 19000000 INFO @ Tue, 16 Jun 2020 11:05:54: 14000000 INFO @ Tue, 16 Jun 2020 11:05:55: 26000000 INFO @ Tue, 16 Jun 2020 11:05:56: 20000000 INFO @ Tue, 16 Jun 2020 11:05:59: 15000000 INFO @ Tue, 16 Jun 2020 11:06:01: 27000000 INFO @ Tue, 16 Jun 2020 11:06:01: 21000000 INFO @ Tue, 16 Jun 2020 11:06:05: 16000000 INFO @ Tue, 16 Jun 2020 11:06:07: 28000000 INFO @ Tue, 16 Jun 2020 11:06:07: 22000000 INFO @ Tue, 16 Jun 2020 11:06:10: 17000000 INFO @ Tue, 16 Jun 2020 11:06:12: 29000000 INFO @ Tue, 16 Jun 2020 11:06:12: 23000000 INFO @ Tue, 16 Jun 2020 11:06:16: 18000000 INFO @ Tue, 16 Jun 2020 11:06:17: 30000000 INFO @ Tue, 16 Jun 2020 11:06:18: 24000000 INFO @ Tue, 16 Jun 2020 11:06:21: 19000000 INFO @ Tue, 16 Jun 2020 11:06:23: 31000000 INFO @ Tue, 16 Jun 2020 11:06:23: 25000000 INFO @ Tue, 16 Jun 2020 11:06:27: 20000000 INFO @ Tue, 16 Jun 2020 11:06:28: 32000000 INFO @ Tue, 16 Jun 2020 11:06:29: 26000000 INFO @ Tue, 16 Jun 2020 11:06:32: 21000000 INFO @ Tue, 16 Jun 2020 11:06:34: 33000000 INFO @ Tue, 16 Jun 2020 11:06:35: 27000000 INFO @ Tue, 16 Jun 2020 11:06:38: 22000000 INFO @ Tue, 16 Jun 2020 11:06:40: 34000000 INFO @ Tue, 16 Jun 2020 11:06:40: 28000000 INFO @ Tue, 16 Jun 2020 11:06:43: 23000000 INFO @ Tue, 16 Jun 2020 11:06:45: 35000000 INFO @ Tue, 16 Jun 2020 11:06:46: 29000000 INFO @ Tue, 16 Jun 2020 11:06:49: 24000000 INFO @ Tue, 16 Jun 2020 11:06:50: 36000000 INFO @ Tue, 16 Jun 2020 11:06:51: 30000000 INFO @ Tue, 16 Jun 2020 11:06:54: 25000000 INFO @ Tue, 16 Jun 2020 11:06:56: 37000000 INFO @ Tue, 16 Jun 2020 11:06:57: 31000000 INFO @ Tue, 16 Jun 2020 11:07:00: 26000000 INFO @ Tue, 16 Jun 2020 11:07:01: 38000000 INFO @ Tue, 16 Jun 2020 11:07:02: 32000000 INFO @ Tue, 16 Jun 2020 11:07:05: 27000000 INFO @ Tue, 16 Jun 2020 11:07:07: 39000000 INFO @ Tue, 16 Jun 2020 11:07:08: 33000000 INFO @ Tue, 16 Jun 2020 11:07:11: 28000000 INFO @ Tue, 16 Jun 2020 11:07:12: 40000000 INFO @ Tue, 16 Jun 2020 11:07:14: 34000000 INFO @ Tue, 16 Jun 2020 11:07:16: 29000000 INFO @ Tue, 16 Jun 2020 11:07:17: 41000000 INFO @ Tue, 16 Jun 2020 11:07:19: 35000000 INFO @ Tue, 16 Jun 2020 11:07:22: 30000000 INFO @ Tue, 16 Jun 2020 11:07:23: 42000000 INFO @ Tue, 16 Jun 2020 11:07:25: 36000000 INFO @ Tue, 16 Jun 2020 11:07:27: 31000000 INFO @ Tue, 16 Jun 2020 11:07:28: 43000000 INFO @ Tue, 16 Jun 2020 11:07:30: 37000000 INFO @ Tue, 16 Jun 2020 11:07:33: 32000000 INFO @ Tue, 16 Jun 2020 11:07:34: #1 tag size is determined as 52 bps INFO @ Tue, 16 Jun 2020 11:07:34: #1 tag size = 52 INFO @ Tue, 16 Jun 2020 11:07:34: #1 total tags in treatment: 43891021 INFO @ Tue, 16 Jun 2020 11:07:34: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 11:07:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 11:07:35: #1 tags after filtering in treatment: 43891021 INFO @ Tue, 16 Jun 2020 11:07:35: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 11:07:35: #1 finished! INFO @ Tue, 16 Jun 2020 11:07:35: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 11:07:35: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 11:07:35: 38000000 INFO @ Tue, 16 Jun 2020 11:07:38: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 11:07:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 11:07:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5702588/SRX5702588.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5702588/SRX5702588.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5702588/SRX5702588.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5702588/SRX5702588.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 11:07:38: 33000000 INFO @ Tue, 16 Jun 2020 11:07:41: 39000000 INFO @ Tue, 16 Jun 2020 11:07:44: 34000000 INFO @ Tue, 16 Jun 2020 11:07:46: 40000000 INFO @ Tue, 16 Jun 2020 11:07:49: 35000000 INFO @ Tue, 16 Jun 2020 11:07:52: 41000000 INFO @ Tue, 16 Jun 2020 11:07:55: 36000000 INFO @ Tue, 16 Jun 2020 11:07:58: 42000000 INFO @ Tue, 16 Jun 2020 11:08:00: 37000000 INFO @ Tue, 16 Jun 2020 11:08:03: 43000000 INFO @ Tue, 16 Jun 2020 11:08:06: 38000000 INFO @ Tue, 16 Jun 2020 11:08:09: #1 tag size is determined as 52 bps INFO @ Tue, 16 Jun 2020 11:08:09: #1 tag size = 52 INFO @ Tue, 16 Jun 2020 11:08:09: #1 total tags in treatment: 43891021 INFO @ Tue, 16 Jun 2020 11:08:09: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 11:08:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 11:08:10: #1 tags after filtering in treatment: 43891021 INFO @ Tue, 16 Jun 2020 11:08:10: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 11:08:10: #1 finished! INFO @ Tue, 16 Jun 2020 11:08:10: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 11:08:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 11:08:11: 39000000 INFO @ Tue, 16 Jun 2020 11:08:13: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 11:08:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 11:08:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5702588/SRX5702588.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5702588/SRX5702588.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5702588/SRX5702588.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5702588/SRX5702588.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 11:08:17: 40000000 INFO @ Tue, 16 Jun 2020 11:08:22: 41000000 INFO @ Tue, 16 Jun 2020 11:08:28: 42000000 INFO @ Tue, 16 Jun 2020 11:08:33: 43000000 INFO @ Tue, 16 Jun 2020 11:08:39: #1 tag size is determined as 52 bps INFO @ Tue, 16 Jun 2020 11:08:39: #1 tag size = 52 INFO @ Tue, 16 Jun 2020 11:08:39: #1 total tags in treatment: 43891021 INFO @ Tue, 16 Jun 2020 11:08:39: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 11:08:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 11:08:40: #1 tags after filtering in treatment: 43891021 INFO @ Tue, 16 Jun 2020 11:08:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 11:08:40: #1 finished! INFO @ Tue, 16 Jun 2020 11:08:40: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 11:08:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 11:08:43: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 11:08:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 11:08:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5702588/SRX5702588.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5702588/SRX5702588.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5702588/SRX5702588.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5702588/SRX5702588.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。