Job ID = 6368760 SRX = SRX5545245 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:29:58 prefetch.2.10.7: 1) Downloading 'SRR8754489'... 2020-06-16T00:29:58 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:36:46 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:36:46 prefetch.2.10.7: 1) 'SRR8754489' was downloaded successfully 2020-06-16T00:36:46 prefetch.2.10.7: 'SRR8754489' has 0 unresolved dependencies Read 83662833 spots for SRR8754489/SRR8754489.sra Written 83662833 spots for SRR8754489/SRR8754489.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:09 83662833 reads; of these: 83662833 (100.00%) were unpaired; of these: 5962380 (7.13%) aligned 0 times 65206988 (77.94%) aligned exactly 1 time 12493465 (14.93%) aligned >1 times 92.87% overall alignment rate Time searching: 00:19:09 Overall time: 00:19:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 28009016 / 77700453 = 0.3605 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:13:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5545245/SRX5545245.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5545245/SRX5545245.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5545245/SRX5545245.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5545245/SRX5545245.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:13:34: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:13:34: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:13:40: 1000000 INFO @ Tue, 16 Jun 2020 10:13:46: 2000000 INFO @ Tue, 16 Jun 2020 10:13:51: 3000000 INFO @ Tue, 16 Jun 2020 10:13:57: 4000000 INFO @ Tue, 16 Jun 2020 10:14:02: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:14:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5545245/SRX5545245.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5545245/SRX5545245.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5545245/SRX5545245.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5545245/SRX5545245.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:14:04: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:14:04: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:14:08: 6000000 INFO @ Tue, 16 Jun 2020 10:14:10: 1000000 INFO @ Tue, 16 Jun 2020 10:14:13: 7000000 INFO @ Tue, 16 Jun 2020 10:14:16: 2000000 INFO @ Tue, 16 Jun 2020 10:14:19: 8000000 INFO @ Tue, 16 Jun 2020 10:14:21: 3000000 INFO @ Tue, 16 Jun 2020 10:14:25: 9000000 INFO @ Tue, 16 Jun 2020 10:14:27: 4000000 INFO @ Tue, 16 Jun 2020 10:14:30: 10000000 INFO @ Tue, 16 Jun 2020 10:14:32: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:14:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5545245/SRX5545245.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5545245/SRX5545245.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5545245/SRX5545245.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5545245/SRX5545245.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:14:34: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:14:34: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:14:36: 11000000 INFO @ Tue, 16 Jun 2020 10:14:38: 6000000 INFO @ Tue, 16 Jun 2020 10:14:40: 1000000 INFO @ Tue, 16 Jun 2020 10:14:41: 12000000 INFO @ Tue, 16 Jun 2020 10:14:43: 7000000 INFO @ Tue, 16 Jun 2020 10:14:46: 2000000 INFO @ Tue, 16 Jun 2020 10:14:47: 13000000 INFO @ Tue, 16 Jun 2020 10:14:49: 8000000 INFO @ Tue, 16 Jun 2020 10:14:52: 3000000 INFO @ Tue, 16 Jun 2020 10:14:52: 14000000 INFO @ Tue, 16 Jun 2020 10:14:55: 9000000 INFO @ Tue, 16 Jun 2020 10:14:58: 4000000 INFO @ Tue, 16 Jun 2020 10:14:58: 15000000 INFO @ Tue, 16 Jun 2020 10:15:00: 10000000 INFO @ Tue, 16 Jun 2020 10:15:03: 5000000 INFO @ Tue, 16 Jun 2020 10:15:04: 16000000 INFO @ Tue, 16 Jun 2020 10:15:06: 11000000 INFO @ Tue, 16 Jun 2020 10:15:09: 6000000 INFO @ Tue, 16 Jun 2020 10:15:09: 17000000 INFO @ Tue, 16 Jun 2020 10:15:11: 12000000 INFO @ Tue, 16 Jun 2020 10:15:15: 18000000 INFO @ Tue, 16 Jun 2020 10:15:15: 7000000 INFO @ Tue, 16 Jun 2020 10:15:17: 13000000 INFO @ Tue, 16 Jun 2020 10:15:21: 19000000 INFO @ Tue, 16 Jun 2020 10:15:21: 8000000 INFO @ Tue, 16 Jun 2020 10:15:22: 14000000 INFO @ Tue, 16 Jun 2020 10:15:26: 20000000 INFO @ Tue, 16 Jun 2020 10:15:27: 9000000 INFO @ Tue, 16 Jun 2020 10:15:28: 15000000 INFO @ Tue, 16 Jun 2020 10:15:32: 21000000 INFO @ Tue, 16 Jun 2020 10:15:32: 10000000 INFO @ Tue, 16 Jun 2020 10:15:33: 16000000 INFO @ Tue, 16 Jun 2020 10:15:37: 22000000 INFO @ Tue, 16 Jun 2020 10:15:38: 11000000 INFO @ Tue, 16 Jun 2020 10:15:39: 17000000 INFO @ Tue, 16 Jun 2020 10:15:43: 23000000 INFO @ Tue, 16 Jun 2020 10:15:44: 12000000 INFO @ Tue, 16 Jun 2020 10:15:45: 18000000 INFO @ Tue, 16 Jun 2020 10:15:49: 24000000 INFO @ Tue, 16 Jun 2020 10:15:49: 13000000 INFO @ Tue, 16 Jun 2020 10:15:50: 19000000 INFO @ Tue, 16 Jun 2020 10:15:54: 25000000 INFO @ Tue, 16 Jun 2020 10:15:55: 14000000 INFO @ Tue, 16 Jun 2020 10:15:56: 20000000 INFO @ Tue, 16 Jun 2020 10:16:00: 26000000 INFO @ Tue, 16 Jun 2020 10:16:01: 15000000 INFO @ Tue, 16 Jun 2020 10:16:01: 21000000 INFO @ Tue, 16 Jun 2020 10:16:05: 27000000 INFO @ Tue, 16 Jun 2020 10:16:06: 16000000 INFO @ Tue, 16 Jun 2020 10:16:07: 22000000 INFO @ Tue, 16 Jun 2020 10:16:11: 28000000 INFO @ Tue, 16 Jun 2020 10:16:12: 17000000 INFO @ Tue, 16 Jun 2020 10:16:12: 23000000 INFO @ Tue, 16 Jun 2020 10:16:16: 29000000 INFO @ Tue, 16 Jun 2020 10:16:18: 18000000 INFO @ Tue, 16 Jun 2020 10:16:18: 24000000 INFO @ Tue, 16 Jun 2020 10:16:22: 30000000 INFO @ Tue, 16 Jun 2020 10:16:23: 25000000 INFO @ Tue, 16 Jun 2020 10:16:23: 19000000 INFO @ Tue, 16 Jun 2020 10:16:27: 31000000 INFO @ Tue, 16 Jun 2020 10:16:29: 26000000 INFO @ Tue, 16 Jun 2020 10:16:29: 20000000 INFO @ Tue, 16 Jun 2020 10:16:33: 32000000 INFO @ Tue, 16 Jun 2020 10:16:34: 27000000 INFO @ Tue, 16 Jun 2020 10:16:35: 21000000 INFO @ Tue, 16 Jun 2020 10:16:38: 33000000 INFO @ Tue, 16 Jun 2020 10:16:40: 28000000 INFO @ Tue, 16 Jun 2020 10:16:40: 22000000 INFO @ Tue, 16 Jun 2020 10:16:44: 34000000 INFO @ Tue, 16 Jun 2020 10:16:45: 29000000 INFO @ Tue, 16 Jun 2020 10:16:46: 23000000 INFO @ Tue, 16 Jun 2020 10:16:49: 35000000 INFO @ Tue, 16 Jun 2020 10:16:50: 30000000 INFO @ Tue, 16 Jun 2020 10:16:51: 24000000 INFO @ Tue, 16 Jun 2020 10:16:55: 36000000 INFO @ Tue, 16 Jun 2020 10:16:56: 31000000 INFO @ Tue, 16 Jun 2020 10:16:57: 25000000 INFO @ Tue, 16 Jun 2020 10:17:00: 37000000 INFO @ Tue, 16 Jun 2020 10:17:01: 32000000 INFO @ Tue, 16 Jun 2020 10:17:03: 26000000 INFO @ Tue, 16 Jun 2020 10:17:06: 38000000 INFO @ Tue, 16 Jun 2020 10:17:07: 33000000 INFO @ Tue, 16 Jun 2020 10:17:08: 27000000 INFO @ Tue, 16 Jun 2020 10:17:12: 39000000 INFO @ Tue, 16 Jun 2020 10:17:12: 34000000 INFO @ Tue, 16 Jun 2020 10:17:13: 28000000 INFO @ Tue, 16 Jun 2020 10:17:17: 40000000 INFO @ Tue, 16 Jun 2020 10:17:17: 35000000 INFO @ Tue, 16 Jun 2020 10:17:19: 29000000 INFO @ Tue, 16 Jun 2020 10:17:23: 41000000 INFO @ Tue, 16 Jun 2020 10:17:23: 36000000 INFO @ Tue, 16 Jun 2020 10:17:25: 30000000 INFO @ Tue, 16 Jun 2020 10:17:28: 42000000 INFO @ Tue, 16 Jun 2020 10:17:28: 37000000 INFO @ Tue, 16 Jun 2020 10:17:30: 31000000 INFO @ Tue, 16 Jun 2020 10:17:34: 43000000 INFO @ Tue, 16 Jun 2020 10:17:34: 38000000 INFO @ Tue, 16 Jun 2020 10:17:36: 32000000 INFO @ Tue, 16 Jun 2020 10:17:39: 44000000 INFO @ Tue, 16 Jun 2020 10:17:39: 39000000 INFO @ Tue, 16 Jun 2020 10:17:41: 33000000 INFO @ Tue, 16 Jun 2020 10:17:45: 40000000 INFO @ Tue, 16 Jun 2020 10:17:45: 45000000 INFO @ Tue, 16 Jun 2020 10:17:47: 34000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:17:50: 41000000 INFO @ Tue, 16 Jun 2020 10:17:50: 46000000 INFO @ Tue, 16 Jun 2020 10:17:52: 35000000 INFO @ Tue, 16 Jun 2020 10:17:55: 42000000 INFO @ Tue, 16 Jun 2020 10:17:56: 47000000 INFO @ Tue, 16 Jun 2020 10:17:58: 36000000 INFO @ Tue, 16 Jun 2020 10:18:01: 43000000 INFO @ Tue, 16 Jun 2020 10:18:01: 48000000 INFO @ Tue, 16 Jun 2020 10:18:04: 37000000 INFO @ Tue, 16 Jun 2020 10:18:06: 44000000 INFO @ Tue, 16 Jun 2020 10:18:07: 49000000 INFO @ Tue, 16 Jun 2020 10:18:09: 38000000 INFO @ Tue, 16 Jun 2020 10:18:12: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:18:12: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:18:12: #1 total tags in treatment: 49691437 INFO @ Tue, 16 Jun 2020 10:18:12: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:18:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:18:12: 45000000 INFO @ Tue, 16 Jun 2020 10:18:12: #1 tags after filtering in treatment: 49691437 INFO @ Tue, 16 Jun 2020 10:18:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:18:12: #1 finished! INFO @ Tue, 16 Jun 2020 10:18:12: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:18:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:18:15: 39000000 INFO @ Tue, 16 Jun 2020 10:18:16: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:18:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:18:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5545245/SRX5545245.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545245/SRX5545245.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545245/SRX5545245.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545245/SRX5545245.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:18:17: 46000000 INFO @ Tue, 16 Jun 2020 10:18:20: 40000000 INFO @ Tue, 16 Jun 2020 10:18:23: 47000000 INFO @ Tue, 16 Jun 2020 10:18:26: 41000000 INFO @ Tue, 16 Jun 2020 10:18:28: 48000000 INFO @ Tue, 16 Jun 2020 10:18:31: 42000000 INFO @ Tue, 16 Jun 2020 10:18:34: 49000000 INFO @ Tue, 16 Jun 2020 10:18:37: 43000000 INFO @ Tue, 16 Jun 2020 10:18:38: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:18:38: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:18:38: #1 total tags in treatment: 49691437 INFO @ Tue, 16 Jun 2020 10:18:38: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:18:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:18:39: #1 tags after filtering in treatment: 49691437 INFO @ Tue, 16 Jun 2020 10:18:39: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:18:39: #1 finished! INFO @ Tue, 16 Jun 2020 10:18:39: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:18:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:18:42: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:18:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:18:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5545245/SRX5545245.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545245/SRX5545245.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545245/SRX5545245.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545245/SRX5545245.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:18:43: 44000000 INFO @ Tue, 16 Jun 2020 10:18:48: 45000000 INFO @ Tue, 16 Jun 2020 10:18:54: 46000000 INFO @ Tue, 16 Jun 2020 10:19:00: 47000000 INFO @ Tue, 16 Jun 2020 10:19:05: 48000000 INFO @ Tue, 16 Jun 2020 10:19:11: 49000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 10:19:15: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:19:15: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:19:15: #1 total tags in treatment: 49691437 INFO @ Tue, 16 Jun 2020 10:19:15: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:19:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:19:16: #1 tags after filtering in treatment: 49691437 INFO @ Tue, 16 Jun 2020 10:19:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:19:16: #1 finished! INFO @ Tue, 16 Jun 2020 10:19:16: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:19:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:19:19: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:19:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:19:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5545245/SRX5545245.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545245/SRX5545245.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545245/SRX5545245.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545245/SRX5545245.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling