Job ID = 6368759 SRX = SRX5545244 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:29:04 prefetch.2.10.7: 1) Downloading 'SRR8754488'... 2020-06-16T00:29:04 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:34:07 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:34:07 prefetch.2.10.7: 1) 'SRR8754488' was downloaded successfully 2020-06-16T00:34:07 prefetch.2.10.7: 'SRR8754488' has 0 unresolved dependencies Read 90606086 spots for SRR8754488/SRR8754488.sra Written 90606086 spots for SRR8754488/SRR8754488.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:20 90606086 reads; of these: 90606086 (100.00%) were unpaired; of these: 3384642 (3.74%) aligned 0 times 73826573 (81.48%) aligned exactly 1 time 13394871 (14.78%) aligned >1 times 96.26% overall alignment rate Time searching: 00:20:20 Overall time: 00:20:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 36 files... [bam_rmdupse_core] 32116928 / 87221444 = 0.3682 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:13:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5545244/SRX5545244.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5545244/SRX5545244.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5545244/SRX5545244.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5545244/SRX5545244.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:13:13: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:13:13: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:13:20: 1000000 INFO @ Tue, 16 Jun 2020 10:13:27: 2000000 INFO @ Tue, 16 Jun 2020 10:13:34: 3000000 INFO @ Tue, 16 Jun 2020 10:13:41: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:13:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5545244/SRX5545244.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5545244/SRX5545244.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5545244/SRX5545244.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5545244/SRX5545244.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:13:43: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:13:43: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:13:48: 5000000 INFO @ Tue, 16 Jun 2020 10:13:50: 1000000 INFO @ Tue, 16 Jun 2020 10:13:56: 6000000 INFO @ Tue, 16 Jun 2020 10:13:57: 2000000 INFO @ Tue, 16 Jun 2020 10:14:04: 7000000 INFO @ Tue, 16 Jun 2020 10:14:04: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:14:11: 4000000 INFO @ Tue, 16 Jun 2020 10:14:11: 8000000 INFO @ Tue, 16 Jun 2020 10:14:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5545244/SRX5545244.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5545244/SRX5545244.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5545244/SRX5545244.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5545244/SRX5545244.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:14:13: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:14:13: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:14:18: 5000000 INFO @ Tue, 16 Jun 2020 10:14:19: 9000000 INFO @ Tue, 16 Jun 2020 10:14:21: 1000000 INFO @ Tue, 16 Jun 2020 10:14:25: 6000000 INFO @ Tue, 16 Jun 2020 10:14:27: 10000000 INFO @ Tue, 16 Jun 2020 10:14:28: 2000000 INFO @ Tue, 16 Jun 2020 10:14:33: 7000000 INFO @ Tue, 16 Jun 2020 10:14:34: 11000000 INFO @ Tue, 16 Jun 2020 10:14:36: 3000000 INFO @ Tue, 16 Jun 2020 10:14:40: 8000000 INFO @ Tue, 16 Jun 2020 10:14:41: 12000000 INFO @ Tue, 16 Jun 2020 10:14:44: 4000000 INFO @ Tue, 16 Jun 2020 10:14:47: 9000000 INFO @ Tue, 16 Jun 2020 10:14:48: 13000000 INFO @ Tue, 16 Jun 2020 10:14:52: 5000000 INFO @ Tue, 16 Jun 2020 10:14:54: 10000000 INFO @ Tue, 16 Jun 2020 10:14:56: 14000000 INFO @ Tue, 16 Jun 2020 10:14:59: 6000000 INFO @ Tue, 16 Jun 2020 10:15:02: 11000000 INFO @ Tue, 16 Jun 2020 10:15:04: 15000000 INFO @ Tue, 16 Jun 2020 10:15:07: 7000000 INFO @ Tue, 16 Jun 2020 10:15:10: 12000000 INFO @ Tue, 16 Jun 2020 10:15:12: 16000000 INFO @ Tue, 16 Jun 2020 10:15:15: 8000000 INFO @ Tue, 16 Jun 2020 10:15:18: 13000000 INFO @ Tue, 16 Jun 2020 10:15:19: 17000000 INFO @ Tue, 16 Jun 2020 10:15:22: 9000000 INFO @ Tue, 16 Jun 2020 10:15:25: 14000000 INFO @ Tue, 16 Jun 2020 10:15:27: 18000000 INFO @ Tue, 16 Jun 2020 10:15:30: 10000000 INFO @ Tue, 16 Jun 2020 10:15:32: 15000000 INFO @ Tue, 16 Jun 2020 10:15:35: 19000000 INFO @ Tue, 16 Jun 2020 10:15:37: 11000000 INFO @ Tue, 16 Jun 2020 10:15:39: 16000000 INFO @ Tue, 16 Jun 2020 10:15:43: 20000000 INFO @ Tue, 16 Jun 2020 10:15:44: 12000000 INFO @ Tue, 16 Jun 2020 10:15:47: 17000000 INFO @ Tue, 16 Jun 2020 10:15:50: 21000000 INFO @ Tue, 16 Jun 2020 10:15:51: 13000000 INFO @ Tue, 16 Jun 2020 10:15:54: 18000000 INFO @ Tue, 16 Jun 2020 10:15:58: 22000000 INFO @ Tue, 16 Jun 2020 10:15:59: 14000000 INFO @ Tue, 16 Jun 2020 10:16:01: 19000000 INFO @ Tue, 16 Jun 2020 10:16:06: 23000000 INFO @ Tue, 16 Jun 2020 10:16:06: 15000000 INFO @ Tue, 16 Jun 2020 10:16:08: 20000000 INFO @ Tue, 16 Jun 2020 10:16:13: 24000000 INFO @ Tue, 16 Jun 2020 10:16:14: 16000000 INFO @ Tue, 16 Jun 2020 10:16:15: 21000000 INFO @ Tue, 16 Jun 2020 10:16:21: 25000000 INFO @ Tue, 16 Jun 2020 10:16:22: 17000000 INFO @ Tue, 16 Jun 2020 10:16:22: 22000000 INFO @ Tue, 16 Jun 2020 10:16:28: 26000000 INFO @ Tue, 16 Jun 2020 10:16:29: 23000000 INFO @ Tue, 16 Jun 2020 10:16:29: 18000000 INFO @ Tue, 16 Jun 2020 10:16:36: 27000000 INFO @ Tue, 16 Jun 2020 10:16:36: 24000000 INFO @ Tue, 16 Jun 2020 10:16:37: 19000000 INFO @ Tue, 16 Jun 2020 10:16:43: 25000000 INFO @ Tue, 16 Jun 2020 10:16:43: 28000000 INFO @ Tue, 16 Jun 2020 10:16:44: 20000000 INFO @ Tue, 16 Jun 2020 10:16:50: 26000000 INFO @ Tue, 16 Jun 2020 10:16:51: 29000000 INFO @ Tue, 16 Jun 2020 10:16:52: 21000000 INFO @ Tue, 16 Jun 2020 10:16:57: 27000000 INFO @ Tue, 16 Jun 2020 10:16:58: 30000000 INFO @ Tue, 16 Jun 2020 10:16:59: 22000000 INFO @ Tue, 16 Jun 2020 10:17:04: 28000000 INFO @ Tue, 16 Jun 2020 10:17:05: 31000000 INFO @ Tue, 16 Jun 2020 10:17:07: 23000000 INFO @ Tue, 16 Jun 2020 10:17:11: 29000000 INFO @ Tue, 16 Jun 2020 10:17:13: 32000000 INFO @ Tue, 16 Jun 2020 10:17:14: 24000000 INFO @ Tue, 16 Jun 2020 10:17:18: 30000000 INFO @ Tue, 16 Jun 2020 10:17:20: 33000000 INFO @ Tue, 16 Jun 2020 10:17:22: 25000000 INFO @ Tue, 16 Jun 2020 10:17:25: 31000000 INFO @ Tue, 16 Jun 2020 10:17:28: 34000000 INFO @ Tue, 16 Jun 2020 10:17:30: 26000000 INFO @ Tue, 16 Jun 2020 10:17:32: 32000000 INFO @ Tue, 16 Jun 2020 10:17:35: 35000000 INFO @ Tue, 16 Jun 2020 10:17:37: 27000000 INFO @ Tue, 16 Jun 2020 10:17:39: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:17:43: 36000000 INFO @ Tue, 16 Jun 2020 10:17:44: 28000000 INFO @ Tue, 16 Jun 2020 10:17:46: 34000000 INFO @ Tue, 16 Jun 2020 10:17:50: 37000000 INFO @ Tue, 16 Jun 2020 10:17:52: 29000000 INFO @ Tue, 16 Jun 2020 10:17:53: 35000000 INFO @ Tue, 16 Jun 2020 10:17:57: 38000000 INFO @ Tue, 16 Jun 2020 10:17:59: 30000000 INFO @ Tue, 16 Jun 2020 10:18:00: 36000000 INFO @ Tue, 16 Jun 2020 10:18:05: 39000000 INFO @ Tue, 16 Jun 2020 10:18:07: 37000000 INFO @ Tue, 16 Jun 2020 10:18:07: 31000000 INFO @ Tue, 16 Jun 2020 10:18:12: 40000000 INFO @ Tue, 16 Jun 2020 10:18:14: 38000000 INFO @ Tue, 16 Jun 2020 10:18:14: 32000000 INFO @ Tue, 16 Jun 2020 10:18:20: 41000000 INFO @ Tue, 16 Jun 2020 10:18:21: 39000000 INFO @ Tue, 16 Jun 2020 10:18:22: 33000000 INFO @ Tue, 16 Jun 2020 10:18:27: 42000000 INFO @ Tue, 16 Jun 2020 10:18:28: 40000000 INFO @ Tue, 16 Jun 2020 10:18:29: 34000000 INFO @ Tue, 16 Jun 2020 10:18:35: 43000000 INFO @ Tue, 16 Jun 2020 10:18:35: 41000000 INFO @ Tue, 16 Jun 2020 10:18:37: 35000000 INFO @ Tue, 16 Jun 2020 10:18:42: 42000000 INFO @ Tue, 16 Jun 2020 10:18:42: 44000000 INFO @ Tue, 16 Jun 2020 10:18:44: 36000000 INFO @ Tue, 16 Jun 2020 10:18:49: 43000000 INFO @ Tue, 16 Jun 2020 10:18:50: 45000000 INFO @ Tue, 16 Jun 2020 10:18:51: 37000000 INFO @ Tue, 16 Jun 2020 10:18:56: 44000000 INFO @ Tue, 16 Jun 2020 10:18:57: 46000000 INFO @ Tue, 16 Jun 2020 10:18:59: 38000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 10:19:03: 45000000 INFO @ Tue, 16 Jun 2020 10:19:04: 47000000 INFO @ Tue, 16 Jun 2020 10:19:06: 39000000 INFO @ Tue, 16 Jun 2020 10:19:10: 46000000 INFO @ Tue, 16 Jun 2020 10:19:12: 48000000 INFO @ Tue, 16 Jun 2020 10:19:14: 40000000 INFO @ Tue, 16 Jun 2020 10:19:17: 47000000 INFO @ Tue, 16 Jun 2020 10:19:19: 49000000 INFO @ Tue, 16 Jun 2020 10:19:22: 41000000 INFO @ Tue, 16 Jun 2020 10:19:24: 48000000 INFO @ Tue, 16 Jun 2020 10:19:27: 50000000 INFO @ Tue, 16 Jun 2020 10:19:29: 42000000 INFO @ Tue, 16 Jun 2020 10:19:31: 49000000 INFO @ Tue, 16 Jun 2020 10:19:35: 51000000 INFO @ Tue, 16 Jun 2020 10:19:37: 43000000 INFO @ Tue, 16 Jun 2020 10:19:39: 50000000 INFO @ Tue, 16 Jun 2020 10:19:42: 52000000 INFO @ Tue, 16 Jun 2020 10:19:45: 44000000 INFO @ Tue, 16 Jun 2020 10:19:46: 51000000 INFO @ Tue, 16 Jun 2020 10:19:50: 53000000 INFO @ Tue, 16 Jun 2020 10:19:52: 45000000 INFO @ Tue, 16 Jun 2020 10:19:53: 52000000 INFO @ Tue, 16 Jun 2020 10:19:57: 54000000 INFO @ Tue, 16 Jun 2020 10:20:00: 53000000 INFO @ Tue, 16 Jun 2020 10:20:00: 46000000 INFO @ Tue, 16 Jun 2020 10:20:05: 55000000 INFO @ Tue, 16 Jun 2020 10:20:06: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:20:06: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:20:06: #1 total tags in treatment: 55104516 INFO @ Tue, 16 Jun 2020 10:20:06: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:20:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:20:07: #1 tags after filtering in treatment: 55104516 INFO @ Tue, 16 Jun 2020 10:20:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:20:07: #1 finished! INFO @ Tue, 16 Jun 2020 10:20:07: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:20:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:20:07: 54000000 INFO @ Tue, 16 Jun 2020 10:20:08: 47000000 INFO @ Tue, 16 Jun 2020 10:20:10: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:20:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:20:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5545244/SRX5545244.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545244/SRX5545244.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545244/SRX5545244.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545244/SRX5545244.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:20:14: 55000000 INFO @ Tue, 16 Jun 2020 10:20:15: 48000000 INFO @ Tue, 16 Jun 2020 10:20:15: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:20:15: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:20:15: #1 total tags in treatment: 55104516 INFO @ Tue, 16 Jun 2020 10:20:15: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:20:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:20:16: #1 tags after filtering in treatment: 55104516 INFO @ Tue, 16 Jun 2020 10:20:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:20:16: #1 finished! INFO @ Tue, 16 Jun 2020 10:20:16: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:20:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:20:20: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:20:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:20:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5545244/SRX5545244.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545244/SRX5545244.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545244/SRX5545244.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545244/SRX5545244.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:20:22: 49000000 INFO @ Tue, 16 Jun 2020 10:20:29: 50000000 INFO @ Tue, 16 Jun 2020 10:20:36: 51000000 INFO @ Tue, 16 Jun 2020 10:20:42: 52000000 INFO @ Tue, 16 Jun 2020 10:20:49: 53000000 INFO @ Tue, 16 Jun 2020 10:20:56: 54000000 INFO @ Tue, 16 Jun 2020 10:21:02: 55000000 INFO @ Tue, 16 Jun 2020 10:21:03: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:21:03: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:21:03: #1 total tags in treatment: 55104516 INFO @ Tue, 16 Jun 2020 10:21:03: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:21:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:21:04: #1 tags after filtering in treatment: 55104516 INFO @ Tue, 16 Jun 2020 10:21:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:21:04: #1 finished! INFO @ Tue, 16 Jun 2020 10:21:04: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:21:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:21:07: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:21:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:21:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5545244/SRX5545244.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545244/SRX5545244.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545244/SRX5545244.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545244/SRX5545244.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling