Job ID = 6368756 SRX = SRX5545241 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:34:14 prefetch.2.10.7: 1) Downloading 'SRR8754483'... 2020-06-16T00:34:14 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:37:23 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:37:23 prefetch.2.10.7: 1) 'SRR8754483' was downloaded successfully 2020-06-16T00:37:23 prefetch.2.10.7: 'SRR8754483' has 0 unresolved dependencies Read 40503524 spots for SRR8754483/SRR8754483.sra Written 40503524 spots for SRR8754483/SRR8754483.sra 2020-06-16T00:39:40 prefetch.2.10.7: 1) Downloading 'SRR8754484'... 2020-06-16T00:39:40 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:43:19 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:43:19 prefetch.2.10.7: 1) 'SRR8754484' was downloaded successfully 2020-06-16T00:43:19 prefetch.2.10.7: 'SRR8754484' has 0 unresolved dependencies Read 52500853 spots for SRR8754484/SRR8754484.sra Written 52500853 spots for SRR8754484/SRR8754484.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:15 93004377 reads; of these: 93004377 (100.00%) were unpaired; of these: 29425221 (31.64%) aligned 0 times 52546145 (56.50%) aligned exactly 1 time 11033011 (11.86%) aligned >1 times 68.36% overall alignment rate Time searching: 00:17:15 Overall time: 00:17:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 24412622 / 63579156 = 0.3840 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:14:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5545241/SRX5545241.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5545241/SRX5545241.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5545241/SRX5545241.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5545241/SRX5545241.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:14:39: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:14:39: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:14:46: 1000000 INFO @ Tue, 16 Jun 2020 10:14:53: 2000000 INFO @ Tue, 16 Jun 2020 10:14:59: 3000000 INFO @ Tue, 16 Jun 2020 10:15:06: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:15:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5545241/SRX5545241.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5545241/SRX5545241.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5545241/SRX5545241.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5545241/SRX5545241.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:15:09: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:15:09: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:15:14: 5000000 INFO @ Tue, 16 Jun 2020 10:15:16: 1000000 INFO @ Tue, 16 Jun 2020 10:15:21: 6000000 INFO @ Tue, 16 Jun 2020 10:15:24: 2000000 INFO @ Tue, 16 Jun 2020 10:15:29: 7000000 INFO @ Tue, 16 Jun 2020 10:15:31: 3000000 INFO @ Tue, 16 Jun 2020 10:15:36: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:15:38: 4000000 INFO @ Tue, 16 Jun 2020 10:15:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5545241/SRX5545241.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5545241/SRX5545241.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5545241/SRX5545241.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5545241/SRX5545241.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:15:39: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:15:39: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:15:43: 9000000 INFO @ Tue, 16 Jun 2020 10:15:45: 5000000 INFO @ Tue, 16 Jun 2020 10:15:47: 1000000 INFO @ Tue, 16 Jun 2020 10:15:50: 10000000 INFO @ Tue, 16 Jun 2020 10:15:52: 6000000 INFO @ Tue, 16 Jun 2020 10:15:55: 2000000 INFO @ Tue, 16 Jun 2020 10:15:57: 11000000 INFO @ Tue, 16 Jun 2020 10:15:59: 7000000 INFO @ Tue, 16 Jun 2020 10:16:03: 3000000 INFO @ Tue, 16 Jun 2020 10:16:05: 12000000 INFO @ Tue, 16 Jun 2020 10:16:06: 8000000 INFO @ Tue, 16 Jun 2020 10:16:11: 4000000 INFO @ Tue, 16 Jun 2020 10:16:11: 13000000 INFO @ Tue, 16 Jun 2020 10:16:13: 9000000 INFO @ Tue, 16 Jun 2020 10:16:18: 14000000 INFO @ Tue, 16 Jun 2020 10:16:19: 10000000 INFO @ Tue, 16 Jun 2020 10:16:20: 5000000 INFO @ Tue, 16 Jun 2020 10:16:26: 15000000 INFO @ Tue, 16 Jun 2020 10:16:26: 11000000 INFO @ Tue, 16 Jun 2020 10:16:28: 6000000 INFO @ Tue, 16 Jun 2020 10:16:33: 16000000 INFO @ Tue, 16 Jun 2020 10:16:33: 12000000 INFO @ Tue, 16 Jun 2020 10:16:36: 7000000 INFO @ Tue, 16 Jun 2020 10:16:40: 17000000 INFO @ Tue, 16 Jun 2020 10:16:41: 13000000 INFO @ Tue, 16 Jun 2020 10:16:45: 8000000 INFO @ Tue, 16 Jun 2020 10:16:48: 14000000 INFO @ Tue, 16 Jun 2020 10:16:48: 18000000 INFO @ Tue, 16 Jun 2020 10:16:53: 9000000 INFO @ Tue, 16 Jun 2020 10:16:55: 15000000 INFO @ Tue, 16 Jun 2020 10:16:55: 19000000 INFO @ Tue, 16 Jun 2020 10:17:01: 10000000 INFO @ Tue, 16 Jun 2020 10:17:02: 16000000 INFO @ Tue, 16 Jun 2020 10:17:03: 20000000 INFO @ Tue, 16 Jun 2020 10:17:09: 11000000 INFO @ Tue, 16 Jun 2020 10:17:10: 17000000 INFO @ Tue, 16 Jun 2020 10:17:10: 21000000 INFO @ Tue, 16 Jun 2020 10:17:17: 12000000 INFO @ Tue, 16 Jun 2020 10:17:18: 18000000 INFO @ Tue, 16 Jun 2020 10:17:18: 22000000 INFO @ Tue, 16 Jun 2020 10:17:25: 19000000 INFO @ Tue, 16 Jun 2020 10:17:25: 23000000 INFO @ Tue, 16 Jun 2020 10:17:25: 13000000 INFO @ Tue, 16 Jun 2020 10:17:32: 20000000 INFO @ Tue, 16 Jun 2020 10:17:32: 24000000 INFO @ Tue, 16 Jun 2020 10:17:34: 14000000 INFO @ Tue, 16 Jun 2020 10:17:39: 21000000 INFO @ Tue, 16 Jun 2020 10:17:40: 25000000 INFO @ Tue, 16 Jun 2020 10:17:41: 15000000 INFO @ Tue, 16 Jun 2020 10:17:46: 22000000 INFO @ Tue, 16 Jun 2020 10:17:47: 26000000 INFO @ Tue, 16 Jun 2020 10:17:48: 16000000 INFO @ Tue, 16 Jun 2020 10:17:53: 23000000 INFO @ Tue, 16 Jun 2020 10:17:54: 27000000 INFO @ Tue, 16 Jun 2020 10:17:55: 17000000 INFO @ Tue, 16 Jun 2020 10:18:00: 24000000 INFO @ Tue, 16 Jun 2020 10:18:02: 28000000 INFO @ Tue, 16 Jun 2020 10:18:03: 18000000 INFO @ Tue, 16 Jun 2020 10:18:08: 25000000 INFO @ Tue, 16 Jun 2020 10:18:09: 29000000 INFO @ Tue, 16 Jun 2020 10:18:12: 19000000 INFO @ Tue, 16 Jun 2020 10:18:15: 26000000 INFO @ Tue, 16 Jun 2020 10:18:17: 30000000 INFO @ Tue, 16 Jun 2020 10:18:20: 20000000 INFO @ Tue, 16 Jun 2020 10:18:23: 27000000 INFO @ Tue, 16 Jun 2020 10:18:25: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:18:28: 21000000 INFO @ Tue, 16 Jun 2020 10:18:30: 28000000 INFO @ Tue, 16 Jun 2020 10:18:33: 32000000 INFO @ Tue, 16 Jun 2020 10:18:36: 22000000 INFO @ Tue, 16 Jun 2020 10:18:38: 29000000 INFO @ Tue, 16 Jun 2020 10:18:40: 33000000 INFO @ Tue, 16 Jun 2020 10:18:44: 23000000 INFO @ Tue, 16 Jun 2020 10:18:46: 30000000 INFO @ Tue, 16 Jun 2020 10:18:48: 34000000 INFO @ Tue, 16 Jun 2020 10:18:51: 24000000 INFO @ Tue, 16 Jun 2020 10:18:54: 31000000 INFO @ Tue, 16 Jun 2020 10:18:56: 35000000 INFO @ Tue, 16 Jun 2020 10:18:58: 25000000 INFO @ Tue, 16 Jun 2020 10:19:02: 32000000 INFO @ Tue, 16 Jun 2020 10:19:03: 36000000 INFO @ Tue, 16 Jun 2020 10:19:06: 26000000 INFO @ Tue, 16 Jun 2020 10:19:09: 33000000 INFO @ Tue, 16 Jun 2020 10:19:11: 37000000 INFO @ Tue, 16 Jun 2020 10:19:13: 27000000 INFO @ Tue, 16 Jun 2020 10:19:17: 34000000 INFO @ Tue, 16 Jun 2020 10:19:19: 38000000 INFO @ Tue, 16 Jun 2020 10:19:20: 28000000 INFO @ Tue, 16 Jun 2020 10:19:24: 35000000 INFO @ Tue, 16 Jun 2020 10:19:26: 39000000 INFO @ Tue, 16 Jun 2020 10:19:27: 29000000 INFO @ Tue, 16 Jun 2020 10:19:28: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:19:28: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:19:28: #1 total tags in treatment: 39166534 INFO @ Tue, 16 Jun 2020 10:19:28: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:19:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:19:28: #1 tags after filtering in treatment: 39166534 INFO @ Tue, 16 Jun 2020 10:19:28: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:19:28: #1 finished! INFO @ Tue, 16 Jun 2020 10:19:28: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:19:28: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:19:31: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:19:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:19:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5545241/SRX5545241.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545241/SRX5545241.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545241/SRX5545241.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545241/SRX5545241.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:19:32: 36000000 INFO @ Tue, 16 Jun 2020 10:19:34: 30000000 INFO @ Tue, 16 Jun 2020 10:19:40: 37000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 10:19:42: 31000000 INFO @ Tue, 16 Jun 2020 10:19:47: 38000000 INFO @ Tue, 16 Jun 2020 10:19:49: 32000000 INFO @ Tue, 16 Jun 2020 10:19:55: 39000000 INFO @ Tue, 16 Jun 2020 10:19:56: 33000000 INFO @ Tue, 16 Jun 2020 10:19:56: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:19:56: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:19:56: #1 total tags in treatment: 39166534 INFO @ Tue, 16 Jun 2020 10:19:56: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:19:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:19:57: #1 tags after filtering in treatment: 39166534 INFO @ Tue, 16 Jun 2020 10:19:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:19:57: #1 finished! INFO @ Tue, 16 Jun 2020 10:19:57: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:19:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:20:00: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:20:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:20:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5545241/SRX5545241.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545241/SRX5545241.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545241/SRX5545241.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545241/SRX5545241.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:20:03: 34000000 INFO @ Tue, 16 Jun 2020 10:20:10: 35000000 INFO @ Tue, 16 Jun 2020 10:20:16: 36000000 INFO @ Tue, 16 Jun 2020 10:20:23: 37000000 INFO @ Tue, 16 Jun 2020 10:20:29: 38000000 INFO @ Tue, 16 Jun 2020 10:20:36: 39000000 INFO @ Tue, 16 Jun 2020 10:20:37: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:20:37: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:20:37: #1 total tags in treatment: 39166534 INFO @ Tue, 16 Jun 2020 10:20:37: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:20:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:20:37: #1 tags after filtering in treatment: 39166534 INFO @ Tue, 16 Jun 2020 10:20:37: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:20:37: #1 finished! INFO @ Tue, 16 Jun 2020 10:20:37: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:20:37: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:20:40: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:20:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:20:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5545241/SRX5545241.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545241/SRX5545241.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545241/SRX5545241.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545241/SRX5545241.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling