Job ID = 6368755 SRX = SRX5545240 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:28:05 prefetch.2.10.7: 1) Downloading 'SRR8754481'... 2020-06-16T00:28:05 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:30:19 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:30:19 prefetch.2.10.7: 1) 'SRR8754481' was downloaded successfully 2020-06-16T00:30:19 prefetch.2.10.7: 'SRR8754481' has 0 unresolved dependencies Read 32932495 spots for SRR8754481/SRR8754481.sra Written 32932495 spots for SRR8754481/SRR8754481.sra 2020-06-16T00:32:16 prefetch.2.10.7: 1) Downloading 'SRR8754482'... 2020-06-16T00:32:16 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:35:10 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:35:10 prefetch.2.10.7: 1) 'SRR8754482' was downloaded successfully 2020-06-16T00:35:10 prefetch.2.10.7: 'SRR8754482' has 0 unresolved dependencies Read 42540717 spots for SRR8754482/SRR8754482.sra Written 42540717 spots for SRR8754482/SRR8754482.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:48 75473212 reads; of these: 75473212 (100.00%) were unpaired; of these: 13146672 (17.42%) aligned 0 times 52028098 (68.94%) aligned exactly 1 time 10298442 (13.65%) aligned >1 times 82.58% overall alignment rate Time searching: 00:15:48 Overall time: 00:15:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 18062834 / 62326540 = 0.2898 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:04:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5545240/SRX5545240.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5545240/SRX5545240.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5545240/SRX5545240.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5545240/SRX5545240.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:04:43: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:04:43: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:04:48: 1000000 INFO @ Tue, 16 Jun 2020 10:04:53: 2000000 INFO @ Tue, 16 Jun 2020 10:04:58: 3000000 INFO @ Tue, 16 Jun 2020 10:05:03: 4000000 INFO @ Tue, 16 Jun 2020 10:05:08: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:05:13: 6000000 INFO @ Tue, 16 Jun 2020 10:05:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5545240/SRX5545240.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5545240/SRX5545240.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5545240/SRX5545240.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5545240/SRX5545240.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:05:13: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:05:13: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:05:18: 7000000 INFO @ Tue, 16 Jun 2020 10:05:19: 1000000 INFO @ Tue, 16 Jun 2020 10:05:24: 8000000 INFO @ Tue, 16 Jun 2020 10:05:24: 2000000 INFO @ Tue, 16 Jun 2020 10:05:29: 9000000 INFO @ Tue, 16 Jun 2020 10:05:30: 3000000 INFO @ Tue, 16 Jun 2020 10:05:34: 10000000 INFO @ Tue, 16 Jun 2020 10:05:35: 4000000 INFO @ Tue, 16 Jun 2020 10:05:39: 11000000 INFO @ Tue, 16 Jun 2020 10:05:40: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:05:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5545240/SRX5545240.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5545240/SRX5545240.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5545240/SRX5545240.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5545240/SRX5545240.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:05:43: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:05:43: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:05:44: 12000000 INFO @ Tue, 16 Jun 2020 10:05:46: 6000000 INFO @ Tue, 16 Jun 2020 10:05:49: 1000000 INFO @ Tue, 16 Jun 2020 10:05:50: 13000000 INFO @ Tue, 16 Jun 2020 10:05:51: 7000000 INFO @ Tue, 16 Jun 2020 10:05:55: 2000000 INFO @ Tue, 16 Jun 2020 10:05:55: 14000000 INFO @ Tue, 16 Jun 2020 10:05:57: 8000000 INFO @ Tue, 16 Jun 2020 10:06:01: 15000000 INFO @ Tue, 16 Jun 2020 10:06:01: 3000000 INFO @ Tue, 16 Jun 2020 10:06:03: 9000000 INFO @ Tue, 16 Jun 2020 10:06:06: 16000000 INFO @ Tue, 16 Jun 2020 10:06:07: 4000000 INFO @ Tue, 16 Jun 2020 10:06:09: 10000000 INFO @ Tue, 16 Jun 2020 10:06:12: 17000000 INFO @ Tue, 16 Jun 2020 10:06:12: 5000000 INFO @ Tue, 16 Jun 2020 10:06:14: 11000000 INFO @ Tue, 16 Jun 2020 10:06:17: 18000000 INFO @ Tue, 16 Jun 2020 10:06:18: 6000000 INFO @ Tue, 16 Jun 2020 10:06:20: 12000000 INFO @ Tue, 16 Jun 2020 10:06:22: 19000000 INFO @ Tue, 16 Jun 2020 10:06:24: 7000000 INFO @ Tue, 16 Jun 2020 10:06:26: 13000000 INFO @ Tue, 16 Jun 2020 10:06:28: 20000000 INFO @ Tue, 16 Jun 2020 10:06:30: 8000000 INFO @ Tue, 16 Jun 2020 10:06:32: 14000000 INFO @ Tue, 16 Jun 2020 10:06:33: 21000000 INFO @ Tue, 16 Jun 2020 10:06:35: 9000000 INFO @ Tue, 16 Jun 2020 10:06:37: 15000000 INFO @ Tue, 16 Jun 2020 10:06:39: 22000000 INFO @ Tue, 16 Jun 2020 10:06:41: 10000000 INFO @ Tue, 16 Jun 2020 10:06:43: 16000000 INFO @ Tue, 16 Jun 2020 10:06:44: 23000000 INFO @ Tue, 16 Jun 2020 10:06:47: 11000000 INFO @ Tue, 16 Jun 2020 10:06:49: 17000000 INFO @ Tue, 16 Jun 2020 10:06:50: 24000000 INFO @ Tue, 16 Jun 2020 10:06:53: 12000000 INFO @ Tue, 16 Jun 2020 10:06:54: 18000000 INFO @ Tue, 16 Jun 2020 10:06:56: 25000000 INFO @ Tue, 16 Jun 2020 10:06:59: 13000000 INFO @ Tue, 16 Jun 2020 10:07:00: 19000000 INFO @ Tue, 16 Jun 2020 10:07:01: 26000000 INFO @ Tue, 16 Jun 2020 10:07:05: 14000000 INFO @ Tue, 16 Jun 2020 10:07:06: 20000000 INFO @ Tue, 16 Jun 2020 10:07:07: 27000000 INFO @ Tue, 16 Jun 2020 10:07:11: 15000000 INFO @ Tue, 16 Jun 2020 10:07:11: 21000000 INFO @ Tue, 16 Jun 2020 10:07:12: 28000000 INFO @ Tue, 16 Jun 2020 10:07:17: 22000000 INFO @ Tue, 16 Jun 2020 10:07:17: 16000000 INFO @ Tue, 16 Jun 2020 10:07:18: 29000000 INFO @ Tue, 16 Jun 2020 10:07:23: 23000000 INFO @ Tue, 16 Jun 2020 10:07:23: 17000000 INFO @ Tue, 16 Jun 2020 10:07:24: 30000000 INFO @ Tue, 16 Jun 2020 10:07:28: 24000000 INFO @ Tue, 16 Jun 2020 10:07:29: 18000000 INFO @ Tue, 16 Jun 2020 10:07:30: 31000000 INFO @ Tue, 16 Jun 2020 10:07:34: 25000000 INFO @ Tue, 16 Jun 2020 10:07:34: 19000000 INFO @ Tue, 16 Jun 2020 10:07:36: 32000000 INFO @ Tue, 16 Jun 2020 10:07:39: 26000000 INFO @ Tue, 16 Jun 2020 10:07:40: 20000000 INFO @ Tue, 16 Jun 2020 10:07:41: 33000000 INFO @ Tue, 16 Jun 2020 10:07:45: 27000000 INFO @ Tue, 16 Jun 2020 10:07:46: 21000000 INFO @ Tue, 16 Jun 2020 10:07:47: 34000000 INFO @ Tue, 16 Jun 2020 10:07:51: 28000000 INFO @ Tue, 16 Jun 2020 10:07:52: 22000000 INFO @ Tue, 16 Jun 2020 10:07:53: 35000000 INFO @ Tue, 16 Jun 2020 10:07:56: 29000000 INFO @ Tue, 16 Jun 2020 10:07:57: 23000000 INFO @ Tue, 16 Jun 2020 10:07:58: 36000000 INFO @ Tue, 16 Jun 2020 10:08:02: 30000000 INFO @ Tue, 16 Jun 2020 10:08:03: 24000000 INFO @ Tue, 16 Jun 2020 10:08:04: 37000000 INFO @ Tue, 16 Jun 2020 10:08:08: 31000000 INFO @ Tue, 16 Jun 2020 10:08:09: 25000000 INFO @ Tue, 16 Jun 2020 10:08:10: 38000000 INFO @ Tue, 16 Jun 2020 10:08:13: 32000000 INFO @ Tue, 16 Jun 2020 10:08:15: 26000000 INFO @ Tue, 16 Jun 2020 10:08:16: 39000000 INFO @ Tue, 16 Jun 2020 10:08:19: 33000000 INFO @ Tue, 16 Jun 2020 10:08:21: 27000000 INFO @ Tue, 16 Jun 2020 10:08:21: 40000000 INFO @ Tue, 16 Jun 2020 10:08:24: 34000000 INFO @ Tue, 16 Jun 2020 10:08:27: 28000000 INFO @ Tue, 16 Jun 2020 10:08:27: 41000000 INFO @ Tue, 16 Jun 2020 10:08:30: 35000000 INFO @ Tue, 16 Jun 2020 10:08:32: 29000000 INFO @ Tue, 16 Jun 2020 10:08:33: 42000000 INFO @ Tue, 16 Jun 2020 10:08:35: 36000000 INFO @ Tue, 16 Jun 2020 10:08:38: 30000000 INFO @ Tue, 16 Jun 2020 10:08:39: 43000000 INFO @ Tue, 16 Jun 2020 10:08:41: 37000000 INFO @ Tue, 16 Jun 2020 10:08:44: 31000000 INFO @ Tue, 16 Jun 2020 10:08:45: 44000000 INFO @ Tue, 16 Jun 2020 10:08:47: 38000000 INFO @ Tue, 16 Jun 2020 10:08:47: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:08:47: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:08:47: #1 total tags in treatment: 44263706 INFO @ Tue, 16 Jun 2020 10:08:47: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:08:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:08:48: #1 tags after filtering in treatment: 44263706 INFO @ Tue, 16 Jun 2020 10:08:48: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:08:48: #1 finished! INFO @ Tue, 16 Jun 2020 10:08:48: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:08:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:08:50: 32000000 INFO @ Tue, 16 Jun 2020 10:08:51: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:08:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:08:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5545240/SRX5545240.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545240/SRX5545240.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545240/SRX5545240.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545240/SRX5545240.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:08:52: 39000000 INFO @ Tue, 16 Jun 2020 10:08:55: 33000000 INFO @ Tue, 16 Jun 2020 10:08:58: 40000000 INFO @ Tue, 16 Jun 2020 10:09:01: 34000000 INFO @ Tue, 16 Jun 2020 10:09:03: 41000000 INFO @ Tue, 16 Jun 2020 10:09:07: 35000000 INFO @ Tue, 16 Jun 2020 10:09:09: 42000000 INFO @ Tue, 16 Jun 2020 10:09:12: 36000000 INFO @ Tue, 16 Jun 2020 10:09:15: 43000000 INFO @ Tue, 16 Jun 2020 10:09:18: 37000000 INFO @ Tue, 16 Jun 2020 10:09:20: 44000000 INFO @ Tue, 16 Jun 2020 10:09:22: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:09:22: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:09:22: #1 total tags in treatment: 44263706 INFO @ Tue, 16 Jun 2020 10:09:22: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:09:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:09:23: #1 tags after filtering in treatment: 44263706 INFO @ Tue, 16 Jun 2020 10:09:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:09:23: #1 finished! INFO @ Tue, 16 Jun 2020 10:09:23: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:09:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:09:23: 38000000 INFO @ Tue, 16 Jun 2020 10:09:26: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:09:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:09:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5545240/SRX5545240.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545240/SRX5545240.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545240/SRX5545240.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545240/SRX5545240.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:09:29: 39000000 INFO @ Tue, 16 Jun 2020 10:09:34: 40000000 INFO @ Tue, 16 Jun 2020 10:09:39: 41000000 INFO @ Tue, 16 Jun 2020 10:09:45: 42000000 INFO @ Tue, 16 Jun 2020 10:09:50: 43000000 INFO @ Tue, 16 Jun 2020 10:09:55: 44000000 INFO @ Tue, 16 Jun 2020 10:09:57: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:09:57: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:09:57: #1 total tags in treatment: 44263706 INFO @ Tue, 16 Jun 2020 10:09:57: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:09:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:09:58: #1 tags after filtering in treatment: 44263706 INFO @ Tue, 16 Jun 2020 10:09:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:09:58: #1 finished! INFO @ Tue, 16 Jun 2020 10:09:58: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:09:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:10:01: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:10:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:10:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5545240/SRX5545240.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545240/SRX5545240.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545240/SRX5545240.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545240/SRX5545240.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。