Job ID = 6368748 SRX = SRX5402771 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:32:00 prefetch.2.10.7: 1) Downloading 'SRR8603005'... 2020-06-16T00:32:00 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:35:16 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:35:16 prefetch.2.10.7: 1) 'SRR8603005' was downloaded successfully 2020-06-16T00:35:16 prefetch.2.10.7: 'SRR8603005' has 0 unresolved dependencies Read 41576290 spots for SRR8603005/SRR8603005.sra Written 41576290 spots for SRR8603005/SRR8603005.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:47 41576290 reads; of these: 41576290 (100.00%) were unpaired; of these: 180933 (0.44%) aligned 0 times 34409945 (82.76%) aligned exactly 1 time 6985412 (16.80%) aligned >1 times 99.56% overall alignment rate Time searching: 00:09:47 Overall time: 00:09:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8682697 / 41395357 = 0.2098 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:54:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5402771/SRX5402771.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5402771/SRX5402771.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5402771/SRX5402771.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5402771/SRX5402771.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:54:16: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:54:16: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:54:23: 1000000 INFO @ Tue, 16 Jun 2020 09:54:31: 2000000 INFO @ Tue, 16 Jun 2020 09:54:38: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:54:45: 4000000 INFO @ Tue, 16 Jun 2020 09:54:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5402771/SRX5402771.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5402771/SRX5402771.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5402771/SRX5402771.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5402771/SRX5402771.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:54:46: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:54:46: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:54:54: 5000000 INFO @ Tue, 16 Jun 2020 09:54:54: 1000000 INFO @ Tue, 16 Jun 2020 09:55:02: 6000000 INFO @ Tue, 16 Jun 2020 09:55:02: 2000000 INFO @ Tue, 16 Jun 2020 09:55:10: 3000000 INFO @ Tue, 16 Jun 2020 09:55:10: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:55:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5402771/SRX5402771.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5402771/SRX5402771.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5402771/SRX5402771.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5402771/SRX5402771.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:55:16: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:55:16: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:55:18: 4000000 INFO @ Tue, 16 Jun 2020 09:55:18: 8000000 INFO @ Tue, 16 Jun 2020 09:55:24: 1000000 INFO @ Tue, 16 Jun 2020 09:55:26: 5000000 INFO @ Tue, 16 Jun 2020 09:55:27: 9000000 INFO @ Tue, 16 Jun 2020 09:55:32: 2000000 INFO @ Tue, 16 Jun 2020 09:55:34: 6000000 INFO @ Tue, 16 Jun 2020 09:55:35: 10000000 INFO @ Tue, 16 Jun 2020 09:55:40: 3000000 INFO @ Tue, 16 Jun 2020 09:55:42: 7000000 INFO @ Tue, 16 Jun 2020 09:55:43: 11000000 INFO @ Tue, 16 Jun 2020 09:55:48: 4000000 INFO @ Tue, 16 Jun 2020 09:55:51: 8000000 INFO @ Tue, 16 Jun 2020 09:55:51: 12000000 INFO @ Tue, 16 Jun 2020 09:55:56: 5000000 INFO @ Tue, 16 Jun 2020 09:55:58: 9000000 INFO @ Tue, 16 Jun 2020 09:55:59: 13000000 INFO @ Tue, 16 Jun 2020 09:56:04: 6000000 INFO @ Tue, 16 Jun 2020 09:56:07: 10000000 INFO @ Tue, 16 Jun 2020 09:56:08: 14000000 INFO @ Tue, 16 Jun 2020 09:56:12: 7000000 INFO @ Tue, 16 Jun 2020 09:56:15: 11000000 INFO @ Tue, 16 Jun 2020 09:56:16: 15000000 INFO @ Tue, 16 Jun 2020 09:56:20: 8000000 INFO @ Tue, 16 Jun 2020 09:56:23: 12000000 INFO @ Tue, 16 Jun 2020 09:56:24: 16000000 INFO @ Tue, 16 Jun 2020 09:56:28: 9000000 INFO @ Tue, 16 Jun 2020 09:56:31: 13000000 INFO @ Tue, 16 Jun 2020 09:56:32: 17000000 INFO @ Tue, 16 Jun 2020 09:56:36: 10000000 INFO @ Tue, 16 Jun 2020 09:56:39: 14000000 INFO @ Tue, 16 Jun 2020 09:56:40: 18000000 INFO @ Tue, 16 Jun 2020 09:56:44: 11000000 INFO @ Tue, 16 Jun 2020 09:56:47: 15000000 INFO @ Tue, 16 Jun 2020 09:56:49: 19000000 INFO @ Tue, 16 Jun 2020 09:56:52: 12000000 INFO @ Tue, 16 Jun 2020 09:56:55: 16000000 INFO @ Tue, 16 Jun 2020 09:56:57: 20000000 INFO @ Tue, 16 Jun 2020 09:57:00: 13000000 INFO @ Tue, 16 Jun 2020 09:57:03: 17000000 INFO @ Tue, 16 Jun 2020 09:57:05: 21000000 INFO @ Tue, 16 Jun 2020 09:57:08: 14000000 INFO @ Tue, 16 Jun 2020 09:57:11: 18000000 INFO @ Tue, 16 Jun 2020 09:57:12: 22000000 INFO @ Tue, 16 Jun 2020 09:57:16: 15000000 INFO @ Tue, 16 Jun 2020 09:57:20: 19000000 INFO @ Tue, 16 Jun 2020 09:57:20: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:57:24: 16000000 INFO @ Tue, 16 Jun 2020 09:57:28: 20000000 INFO @ Tue, 16 Jun 2020 09:57:28: 24000000 INFO @ Tue, 16 Jun 2020 09:57:32: 17000000 INFO @ Tue, 16 Jun 2020 09:57:36: 21000000 INFO @ Tue, 16 Jun 2020 09:57:36: 25000000 INFO @ Tue, 16 Jun 2020 09:57:40: 18000000 INFO @ Tue, 16 Jun 2020 09:57:43: 22000000 INFO @ Tue, 16 Jun 2020 09:57:44: 26000000 INFO @ Tue, 16 Jun 2020 09:57:48: 19000000 INFO @ Tue, 16 Jun 2020 09:57:51: 23000000 INFO @ Tue, 16 Jun 2020 09:57:52: 27000000 INFO @ Tue, 16 Jun 2020 09:57:56: 20000000 INFO @ Tue, 16 Jun 2020 09:57:59: 24000000 INFO @ Tue, 16 Jun 2020 09:58:00: 28000000 INFO @ Tue, 16 Jun 2020 09:58:04: 21000000 INFO @ Tue, 16 Jun 2020 09:58:07: 25000000 INFO @ Tue, 16 Jun 2020 09:58:09: 29000000 INFO @ Tue, 16 Jun 2020 09:58:12: 22000000 INFO @ Tue, 16 Jun 2020 09:58:15: 26000000 INFO @ Tue, 16 Jun 2020 09:58:17: 30000000 INFO @ Tue, 16 Jun 2020 09:58:20: 23000000 INFO @ Tue, 16 Jun 2020 09:58:23: 27000000 INFO @ Tue, 16 Jun 2020 09:58:25: 31000000 INFO @ Tue, 16 Jun 2020 09:58:28: 24000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:58:31: 28000000 INFO @ Tue, 16 Jun 2020 09:58:34: 32000000 INFO @ Tue, 16 Jun 2020 09:58:37: 25000000 INFO @ Tue, 16 Jun 2020 09:58:39: 29000000 INFO @ Tue, 16 Jun 2020 09:58:40: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 09:58:40: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 09:58:40: #1 total tags in treatment: 32712660 INFO @ Tue, 16 Jun 2020 09:58:40: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:58:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:58:41: #1 tags after filtering in treatment: 32712660 INFO @ Tue, 16 Jun 2020 09:58:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:58:41: #1 finished! INFO @ Tue, 16 Jun 2020 09:58:41: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:58:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:58:43: #2 number of paired peaks: 32 WARNING @ Tue, 16 Jun 2020 09:58:43: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:58:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5402771/SRX5402771.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5402771/SRX5402771.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5402771/SRX5402771.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5402771/SRX5402771.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:58:45: 26000000 INFO @ Tue, 16 Jun 2020 09:58:47: 30000000 INFO @ Tue, 16 Jun 2020 09:58:53: 27000000 INFO @ Tue, 16 Jun 2020 09:58:55: 31000000 INFO @ Tue, 16 Jun 2020 09:59:02: 28000000 INFO @ Tue, 16 Jun 2020 09:59:03: 32000000 INFO @ Tue, 16 Jun 2020 09:59:09: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 09:59:09: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 09:59:09: #1 total tags in treatment: 32712660 INFO @ Tue, 16 Jun 2020 09:59:09: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:59:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:59:09: #1 tags after filtering in treatment: 32712660 INFO @ Tue, 16 Jun 2020 09:59:09: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:59:09: #1 finished! INFO @ Tue, 16 Jun 2020 09:59:09: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:59:09: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:59:10: 29000000 INFO @ Tue, 16 Jun 2020 09:59:12: #2 number of paired peaks: 32 WARNING @ Tue, 16 Jun 2020 09:59:12: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:59:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5402771/SRX5402771.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5402771/SRX5402771.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5402771/SRX5402771.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5402771/SRX5402771.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:59:17: 30000000 INFO @ Tue, 16 Jun 2020 09:59:25: 31000000 INFO @ Tue, 16 Jun 2020 09:59:33: 32000000 INFO @ Tue, 16 Jun 2020 09:59:38: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 09:59:38: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 09:59:38: #1 total tags in treatment: 32712660 INFO @ Tue, 16 Jun 2020 09:59:38: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:59:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:59:39: #1 tags after filtering in treatment: 32712660 INFO @ Tue, 16 Jun 2020 09:59:39: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:59:39: #1 finished! INFO @ Tue, 16 Jun 2020 09:59:39: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:59:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:59:41: #2 number of paired peaks: 32 WARNING @ Tue, 16 Jun 2020 09:59:41: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:59:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5402771/SRX5402771.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5402771/SRX5402771.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5402771/SRX5402771.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5402771/SRX5402771.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling