Job ID = 6368695 SRX = SRX5402721 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:26:49 prefetch.2.10.7: 1) Downloading 'SRR8602955'... 2020-06-16T00:26:49 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:29:56 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:29:56 prefetch.2.10.7: 1) 'SRR8602955' was downloaded successfully Read 21718754 spots for SRR8602955/SRR8602955.sra Written 21718754 spots for SRR8602955/SRR8602955.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:14 21718754 reads; of these: 21718754 (100.00%) were unpaired; of these: 3671945 (16.91%) aligned 0 times 15826699 (72.87%) aligned exactly 1 time 2220110 (10.22%) aligned >1 times 83.09% overall alignment rate Time searching: 00:04:14 Overall time: 00:04:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 12349561 / 18046809 = 0.6843 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:38:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5402721/SRX5402721.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5402721/SRX5402721.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5402721/SRX5402721.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5402721/SRX5402721.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:38:55: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:38:55: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:39:01: 1000000 INFO @ Tue, 16 Jun 2020 09:39:08: 2000000 INFO @ Tue, 16 Jun 2020 09:39:14: 3000000 INFO @ Tue, 16 Jun 2020 09:39:21: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:39:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5402721/SRX5402721.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5402721/SRX5402721.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5402721/SRX5402721.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5402721/SRX5402721.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:39:25: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:39:25: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:39:27: 5000000 INFO @ Tue, 16 Jun 2020 09:39:32: 1000000 INFO @ Tue, 16 Jun 2020 09:39:32: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:39:32: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:39:32: #1 total tags in treatment: 5697248 INFO @ Tue, 16 Jun 2020 09:39:32: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:39:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:39:32: #1 tags after filtering in treatment: 5697248 INFO @ Tue, 16 Jun 2020 09:39:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:39:32: #1 finished! INFO @ Tue, 16 Jun 2020 09:39:32: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:39:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:39:33: #2 number of paired peaks: 695 WARNING @ Tue, 16 Jun 2020 09:39:33: Fewer paired peaks (695) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 695 pairs to build model! INFO @ Tue, 16 Jun 2020 09:39:33: start model_add_line... INFO @ Tue, 16 Jun 2020 09:39:33: start X-correlation... INFO @ Tue, 16 Jun 2020 09:39:33: end of X-cor INFO @ Tue, 16 Jun 2020 09:39:33: #2 finished! INFO @ Tue, 16 Jun 2020 09:39:33: #2 predicted fragment length is 52 bps INFO @ Tue, 16 Jun 2020 09:39:33: #2 alternative fragment length(s) may be 3,52 bps INFO @ Tue, 16 Jun 2020 09:39:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX5402721/SRX5402721.05_model.r WARNING @ Tue, 16 Jun 2020 09:39:33: #2 Since the d (52) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 09:39:33: #2 You may need to consider one of the other alternative d(s): 3,52 WARNING @ Tue, 16 Jun 2020 09:39:33: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 09:39:33: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:39:33: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:39:38: 2000000 INFO @ Tue, 16 Jun 2020 09:39:44: 3000000 INFO @ Tue, 16 Jun 2020 09:39:45: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 09:39:51: 4000000 INFO @ Tue, 16 Jun 2020 09:39:51: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX5402721/SRX5402721.05_peaks.xls INFO @ Tue, 16 Jun 2020 09:39:51: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX5402721/SRX5402721.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:39:51: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX5402721/SRX5402721.05_summits.bed INFO @ Tue, 16 Jun 2020 09:39:51: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1002 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:39:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5402721/SRX5402721.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5402721/SRX5402721.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5402721/SRX5402721.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5402721/SRX5402721.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:39:55: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:39:55: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:39:58: 5000000 INFO @ Tue, 16 Jun 2020 09:40:01: 1000000 INFO @ Tue, 16 Jun 2020 09:40:02: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:40:02: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:40:02: #1 total tags in treatment: 5697248 INFO @ Tue, 16 Jun 2020 09:40:02: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:40:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:40:02: #1 tags after filtering in treatment: 5697248 INFO @ Tue, 16 Jun 2020 09:40:02: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:40:02: #1 finished! INFO @ Tue, 16 Jun 2020 09:40:02: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:40:02: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:40:03: #2 number of paired peaks: 695 WARNING @ Tue, 16 Jun 2020 09:40:03: Fewer paired peaks (695) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 695 pairs to build model! INFO @ Tue, 16 Jun 2020 09:40:03: start model_add_line... INFO @ Tue, 16 Jun 2020 09:40:03: start X-correlation... INFO @ Tue, 16 Jun 2020 09:40:03: end of X-cor INFO @ Tue, 16 Jun 2020 09:40:03: #2 finished! INFO @ Tue, 16 Jun 2020 09:40:03: #2 predicted fragment length is 52 bps INFO @ Tue, 16 Jun 2020 09:40:03: #2 alternative fragment length(s) may be 3,52 bps INFO @ Tue, 16 Jun 2020 09:40:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX5402721/SRX5402721.10_model.r WARNING @ Tue, 16 Jun 2020 09:40:03: #2 Since the d (52) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 09:40:03: #2 You may need to consider one of the other alternative d(s): 3,52 WARNING @ Tue, 16 Jun 2020 09:40:03: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 09:40:03: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:40:03: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:40:08: 2000000 INFO @ Tue, 16 Jun 2020 09:40:14: 3000000 INFO @ Tue, 16 Jun 2020 09:40:15: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 09:40:20: 4000000 INFO @ Tue, 16 Jun 2020 09:40:21: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX5402721/SRX5402721.10_peaks.xls INFO @ Tue, 16 Jun 2020 09:40:21: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX5402721/SRX5402721.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:40:21: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX5402721/SRX5402721.10_summits.bed INFO @ Tue, 16 Jun 2020 09:40:21: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (431 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:40:27: 5000000 INFO @ Tue, 16 Jun 2020 09:40:31: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:40:31: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:40:31: #1 total tags in treatment: 5697248 INFO @ Tue, 16 Jun 2020 09:40:31: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:40:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:40:31: #1 tags after filtering in treatment: 5697248 INFO @ Tue, 16 Jun 2020 09:40:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:40:31: #1 finished! INFO @ Tue, 16 Jun 2020 09:40:31: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:40:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:40:32: #2 number of paired peaks: 695 WARNING @ Tue, 16 Jun 2020 09:40:32: Fewer paired peaks (695) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 695 pairs to build model! INFO @ Tue, 16 Jun 2020 09:40:32: start model_add_line... INFO @ Tue, 16 Jun 2020 09:40:32: start X-correlation... INFO @ Tue, 16 Jun 2020 09:40:32: end of X-cor INFO @ Tue, 16 Jun 2020 09:40:32: #2 finished! INFO @ Tue, 16 Jun 2020 09:40:32: #2 predicted fragment length is 52 bps INFO @ Tue, 16 Jun 2020 09:40:32: #2 alternative fragment length(s) may be 3,52 bps INFO @ Tue, 16 Jun 2020 09:40:32: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX5402721/SRX5402721.20_model.r WARNING @ Tue, 16 Jun 2020 09:40:32: #2 Since the d (52) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 09:40:32: #2 You may need to consider one of the other alternative d(s): 3,52 WARNING @ Tue, 16 Jun 2020 09:40:32: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 09:40:32: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:40:32: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:40:44: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 09:40:50: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX5402721/SRX5402721.20_peaks.xls INFO @ Tue, 16 Jun 2020 09:40:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX5402721/SRX5402721.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:40:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX5402721/SRX5402721.20_summits.bed INFO @ Tue, 16 Jun 2020 09:40:50: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (173 records, 4 fields): 2 millis CompletedMACS2peakCalling