Job ID = 6368467 SRX = SRX5020697 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:15:36 prefetch.2.10.7: 1) Downloading 'SRR8201320'... 2020-06-16T00:15:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:17:38 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:17:39 prefetch.2.10.7: 'SRR8201320' is valid 2020-06-16T00:17:39 prefetch.2.10.7: 1) 'SRR8201320' was downloaded successfully Read 9275499 spots for SRR8201320/SRR8201320.sra Written 9275499 spots for SRR8201320/SRR8201320.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:56 9275499 reads; of these: 9275499 (100.00%) were unpaired; of these: 164722 (1.78%) aligned 0 times 7475679 (80.60%) aligned exactly 1 time 1635098 (17.63%) aligned >1 times 98.22% overall alignment rate Time searching: 00:01:56 Overall time: 00:01:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 4369031 / 9110777 = 0.4795 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:23:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5020697/SRX5020697.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5020697/SRX5020697.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5020697/SRX5020697.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5020697/SRX5020697.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:23:09: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:23:09: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:23:15: 1000000 INFO @ Tue, 16 Jun 2020 09:23:21: 2000000 INFO @ Tue, 16 Jun 2020 09:23:27: 3000000 INFO @ Tue, 16 Jun 2020 09:23:32: 4000000 INFO @ Tue, 16 Jun 2020 09:23:37: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 09:23:37: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 09:23:37: #1 total tags in treatment: 4741746 INFO @ Tue, 16 Jun 2020 09:23:37: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:23:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:23:37: #1 tags after filtering in treatment: 4741746 INFO @ Tue, 16 Jun 2020 09:23:37: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:23:37: #1 finished! INFO @ Tue, 16 Jun 2020 09:23:37: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:23:37: #2 looking for paired plus/minus strand peaks... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:23:37: #2 number of paired peaks: 508 WARNING @ Tue, 16 Jun 2020 09:23:37: Fewer paired peaks (508) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 508 pairs to build model! INFO @ Tue, 16 Jun 2020 09:23:37: start model_add_line... INFO @ Tue, 16 Jun 2020 09:23:37: start X-correlation... INFO @ Tue, 16 Jun 2020 09:23:37: end of X-cor INFO @ Tue, 16 Jun 2020 09:23:37: #2 finished! INFO @ Tue, 16 Jun 2020 09:23:37: #2 predicted fragment length is 54 bps INFO @ Tue, 16 Jun 2020 09:23:37: #2 alternative fragment length(s) may be 54,552 bps INFO @ Tue, 16 Jun 2020 09:23:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX5020697/SRX5020697.05_model.r WARNING @ Tue, 16 Jun 2020 09:23:37: #2 Since the d (54) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 09:23:37: #2 You may need to consider one of the other alternative d(s): 54,552 WARNING @ Tue, 16 Jun 2020 09:23:37: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 09:23:37: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:23:37: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:23:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5020697/SRX5020697.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5020697/SRX5020697.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5020697/SRX5020697.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5020697/SRX5020697.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:23:39: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:23:39: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:23:45: 1000000 INFO @ Tue, 16 Jun 2020 09:23:47: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 09:23:51: 2000000 INFO @ Tue, 16 Jun 2020 09:23:51: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX5020697/SRX5020697.05_peaks.xls INFO @ Tue, 16 Jun 2020 09:23:51: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX5020697/SRX5020697.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:23:51: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX5020697/SRX5020697.05_summits.bed INFO @ Tue, 16 Jun 2020 09:23:51: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (621 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:23:57: 3000000 INFO @ Tue, 16 Jun 2020 09:24:03: 4000000 BedGraph に変換中... INFO @ Tue, 16 Jun 2020 09:24:07: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 09:24:07: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 09:24:07: #1 total tags in treatment: 4741746 INFO @ Tue, 16 Jun 2020 09:24:07: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:24:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:24:07: #1 tags after filtering in treatment: 4741746 INFO @ Tue, 16 Jun 2020 09:24:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:24:07: #1 finished! INFO @ Tue, 16 Jun 2020 09:24:07: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:24:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:24:07: #2 number of paired peaks: 508 WARNING @ Tue, 16 Jun 2020 09:24:07: Fewer paired peaks (508) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 508 pairs to build model! INFO @ Tue, 16 Jun 2020 09:24:07: start model_add_line... INFO @ Tue, 16 Jun 2020 09:24:07: start X-correlation... INFO @ Tue, 16 Jun 2020 09:24:07: end of X-cor INFO @ Tue, 16 Jun 2020 09:24:07: #2 finished! INFO @ Tue, 16 Jun 2020 09:24:07: #2 predicted fragment length is 54 bps INFO @ Tue, 16 Jun 2020 09:24:07: #2 alternative fragment length(s) may be 54,552 bps INFO @ Tue, 16 Jun 2020 09:24:07: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX5020697/SRX5020697.10_model.r WARNING @ Tue, 16 Jun 2020 09:24:07: #2 Since the d (54) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 09:24:07: #2 You may need to consider one of the other alternative d(s): 54,552 WARNING @ Tue, 16 Jun 2020 09:24:07: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 09:24:07: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:24:07: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:24:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5020697/SRX5020697.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5020697/SRX5020697.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5020697/SRX5020697.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5020697/SRX5020697.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:24:09: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:24:09: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:24:15: 1000000 INFO @ Tue, 16 Jun 2020 09:24:17: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 09:24:21: 2000000 INFO @ Tue, 16 Jun 2020 09:24:22: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX5020697/SRX5020697.10_peaks.xls INFO @ Tue, 16 Jun 2020 09:24:22: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX5020697/SRX5020697.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:24:22: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX5020697/SRX5020697.10_summits.bed INFO @ Tue, 16 Jun 2020 09:24:22: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (377 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:24:26: 3000000 INFO @ Tue, 16 Jun 2020 09:24:32: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:24:36: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 09:24:36: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 09:24:36: #1 total tags in treatment: 4741746 INFO @ Tue, 16 Jun 2020 09:24:36: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:24:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:24:36: #1 tags after filtering in treatment: 4741746 INFO @ Tue, 16 Jun 2020 09:24:36: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:24:36: #1 finished! INFO @ Tue, 16 Jun 2020 09:24:36: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:24:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:24:37: #2 number of paired peaks: 508 WARNING @ Tue, 16 Jun 2020 09:24:37: Fewer paired peaks (508) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 508 pairs to build model! INFO @ Tue, 16 Jun 2020 09:24:37: start model_add_line... INFO @ Tue, 16 Jun 2020 09:24:37: start X-correlation... INFO @ Tue, 16 Jun 2020 09:24:37: end of X-cor INFO @ Tue, 16 Jun 2020 09:24:37: #2 finished! INFO @ Tue, 16 Jun 2020 09:24:37: #2 predicted fragment length is 54 bps INFO @ Tue, 16 Jun 2020 09:24:37: #2 alternative fragment length(s) may be 54,552 bps INFO @ Tue, 16 Jun 2020 09:24:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX5020697/SRX5020697.20_model.r WARNING @ Tue, 16 Jun 2020 09:24:37: #2 Since the d (54) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 09:24:37: #2 You may need to consider one of the other alternative d(s): 54,552 WARNING @ Tue, 16 Jun 2020 09:24:37: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 09:24:37: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:24:37: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:24:46: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:24:51: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX5020697/SRX5020697.20_peaks.xls INFO @ Tue, 16 Jun 2020 09:24:51: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX5020697/SRX5020697.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:24:51: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX5020697/SRX5020697.20_summits.bed INFO @ Tue, 16 Jun 2020 09:24:51: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (180 records, 4 fields): 1 millis CompletedMACS2peakCalling