Job ID = 6368399 SRX = SRX495078 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:06:39 prefetch.2.10.7: 1) Downloading 'SRR1198610'... 2020-06-16T00:06:39 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:12:06 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:12:06 prefetch.2.10.7: 1) 'SRR1198610' was downloaded successfully Read 47950615 spots for SRR1198610/SRR1198610.sra Written 47950615 spots for SRR1198610/SRR1198610.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:53 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3138693 (6.55%) aligned 0 times 37485870 (78.18%) aligned exactly 1 time 7326052 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:10:53 Overall time: 00:10:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8188951 / 44811922 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:35:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX495078/SRX495078.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX495078/SRX495078.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX495078/SRX495078.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX495078/SRX495078.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:35:49: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:35:49: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:35:56: 1000000 INFO @ Tue, 16 Jun 2020 09:36:02: 2000000 INFO @ Tue, 16 Jun 2020 09:36:08: 3000000 INFO @ Tue, 16 Jun 2020 09:36:15: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:36:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX495078/SRX495078.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX495078/SRX495078.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX495078/SRX495078.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX495078/SRX495078.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:36:19: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:36:19: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:36:21: 5000000 INFO @ Tue, 16 Jun 2020 09:36:27: 1000000 INFO @ Tue, 16 Jun 2020 09:36:28: 6000000 INFO @ Tue, 16 Jun 2020 09:36:34: 2000000 INFO @ Tue, 16 Jun 2020 09:36:36: 7000000 INFO @ Tue, 16 Jun 2020 09:36:41: 3000000 INFO @ Tue, 16 Jun 2020 09:36:43: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:36:48: 4000000 INFO @ Tue, 16 Jun 2020 09:36:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX495078/SRX495078.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX495078/SRX495078.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX495078/SRX495078.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX495078/SRX495078.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:36:49: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:36:49: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:36:50: 9000000 INFO @ Tue, 16 Jun 2020 09:36:55: 5000000 INFO @ Tue, 16 Jun 2020 09:36:56: 1000000 INFO @ Tue, 16 Jun 2020 09:36:57: 10000000 INFO @ Tue, 16 Jun 2020 09:37:02: 6000000 INFO @ Tue, 16 Jun 2020 09:37:03: 2000000 INFO @ Tue, 16 Jun 2020 09:37:05: 11000000 INFO @ Tue, 16 Jun 2020 09:37:09: 3000000 INFO @ Tue, 16 Jun 2020 09:37:10: 7000000 INFO @ Tue, 16 Jun 2020 09:37:12: 12000000 INFO @ Tue, 16 Jun 2020 09:37:16: 4000000 INFO @ Tue, 16 Jun 2020 09:37:17: 8000000 INFO @ Tue, 16 Jun 2020 09:37:19: 13000000 INFO @ Tue, 16 Jun 2020 09:37:22: 5000000 INFO @ Tue, 16 Jun 2020 09:37:24: 9000000 INFO @ Tue, 16 Jun 2020 09:37:27: 14000000 INFO @ Tue, 16 Jun 2020 09:37:29: 6000000 INFO @ Tue, 16 Jun 2020 09:37:31: 10000000 INFO @ Tue, 16 Jun 2020 09:37:34: 15000000 INFO @ Tue, 16 Jun 2020 09:37:36: 7000000 INFO @ Tue, 16 Jun 2020 09:37:39: 11000000 INFO @ Tue, 16 Jun 2020 09:37:42: 16000000 INFO @ Tue, 16 Jun 2020 09:37:42: 8000000 INFO @ Tue, 16 Jun 2020 09:37:46: 12000000 INFO @ Tue, 16 Jun 2020 09:37:49: 9000000 INFO @ Tue, 16 Jun 2020 09:37:49: 17000000 INFO @ Tue, 16 Jun 2020 09:37:53: 13000000 INFO @ Tue, 16 Jun 2020 09:37:56: 10000000 INFO @ Tue, 16 Jun 2020 09:37:56: 18000000 INFO @ Tue, 16 Jun 2020 09:38:00: 14000000 INFO @ Tue, 16 Jun 2020 09:38:02: 11000000 INFO @ Tue, 16 Jun 2020 09:38:04: 19000000 INFO @ Tue, 16 Jun 2020 09:38:07: 15000000 INFO @ Tue, 16 Jun 2020 09:38:09: 12000000 INFO @ Tue, 16 Jun 2020 09:38:11: 20000000 INFO @ Tue, 16 Jun 2020 09:38:15: 16000000 INFO @ Tue, 16 Jun 2020 09:38:15: 13000000 INFO @ Tue, 16 Jun 2020 09:38:19: 21000000 INFO @ Tue, 16 Jun 2020 09:38:22: 17000000 INFO @ Tue, 16 Jun 2020 09:38:22: 14000000 INFO @ Tue, 16 Jun 2020 09:38:26: 22000000 INFO @ Tue, 16 Jun 2020 09:38:28: 15000000 INFO @ Tue, 16 Jun 2020 09:38:29: 18000000 INFO @ Tue, 16 Jun 2020 09:38:34: 23000000 INFO @ Tue, 16 Jun 2020 09:38:35: 16000000 INFO @ Tue, 16 Jun 2020 09:38:36: 19000000 INFO @ Tue, 16 Jun 2020 09:38:42: 17000000 INFO @ Tue, 16 Jun 2020 09:38:42: 24000000 INFO @ Tue, 16 Jun 2020 09:38:44: 20000000 INFO @ Tue, 16 Jun 2020 09:38:48: 18000000 INFO @ Tue, 16 Jun 2020 09:38:49: 25000000 INFO @ Tue, 16 Jun 2020 09:38:51: 21000000 INFO @ Tue, 16 Jun 2020 09:38:55: 19000000 INFO @ Tue, 16 Jun 2020 09:38:57: 26000000 INFO @ Tue, 16 Jun 2020 09:38:58: 22000000 INFO @ Tue, 16 Jun 2020 09:39:02: 20000000 INFO @ Tue, 16 Jun 2020 09:39:05: 27000000 INFO @ Tue, 16 Jun 2020 09:39:05: 23000000 INFO @ Tue, 16 Jun 2020 09:39:08: 21000000 INFO @ Tue, 16 Jun 2020 09:39:12: 28000000 INFO @ Tue, 16 Jun 2020 09:39:13: 24000000 INFO @ Tue, 16 Jun 2020 09:39:15: 22000000 INFO @ Tue, 16 Jun 2020 09:39:20: 29000000 INFO @ Tue, 16 Jun 2020 09:39:20: 25000000 INFO @ Tue, 16 Jun 2020 09:39:22: 23000000 INFO @ Tue, 16 Jun 2020 09:39:27: 26000000 INFO @ Tue, 16 Jun 2020 09:39:28: 30000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:39:28: 24000000 INFO @ Tue, 16 Jun 2020 09:39:35: 25000000 INFO @ Tue, 16 Jun 2020 09:39:35: 27000000 INFO @ Tue, 16 Jun 2020 09:39:35: 31000000 INFO @ Tue, 16 Jun 2020 09:39:42: 26000000 INFO @ Tue, 16 Jun 2020 09:39:43: 28000000 INFO @ Tue, 16 Jun 2020 09:39:43: 32000000 INFO @ Tue, 16 Jun 2020 09:39:48: 27000000 INFO @ Tue, 16 Jun 2020 09:39:50: 29000000 INFO @ Tue, 16 Jun 2020 09:39:51: 33000000 INFO @ Tue, 16 Jun 2020 09:39:55: 28000000 INFO @ Tue, 16 Jun 2020 09:39:58: 30000000 INFO @ Tue, 16 Jun 2020 09:39:58: 34000000 INFO @ Tue, 16 Jun 2020 09:40:02: 29000000 INFO @ Tue, 16 Jun 2020 09:40:06: 31000000 INFO @ Tue, 16 Jun 2020 09:40:06: 35000000 INFO @ Tue, 16 Jun 2020 09:40:09: 30000000 INFO @ Tue, 16 Jun 2020 09:40:13: 32000000 INFO @ Tue, 16 Jun 2020 09:40:14: 36000000 INFO @ Tue, 16 Jun 2020 09:40:16: 31000000 INFO @ Tue, 16 Jun 2020 09:40:19: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:40:19: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:40:19: #1 total tags in treatment: 36622971 INFO @ Tue, 16 Jun 2020 09:40:19: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:40:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:40:19: #1 tags after filtering in treatment: 36622971 INFO @ Tue, 16 Jun 2020 09:40:19: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:40:19: #1 finished! INFO @ Tue, 16 Jun 2020 09:40:19: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:40:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:40:21: 33000000 INFO @ Tue, 16 Jun 2020 09:40:22: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:40:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:40:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX495078/SRX495078.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX495078/SRX495078.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX495078/SRX495078.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX495078/SRX495078.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:40:23: 32000000 INFO @ Tue, 16 Jun 2020 09:40:28: 34000000 INFO @ Tue, 16 Jun 2020 09:40:30: 33000000 INFO @ Tue, 16 Jun 2020 09:40:36: 35000000 INFO @ Tue, 16 Jun 2020 09:40:36: 34000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:40:43: 35000000 INFO @ Tue, 16 Jun 2020 09:40:43: 36000000 INFO @ Tue, 16 Jun 2020 09:40:48: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:40:48: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:40:48: #1 total tags in treatment: 36622971 INFO @ Tue, 16 Jun 2020 09:40:48: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:40:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:40:49: #1 tags after filtering in treatment: 36622971 INFO @ Tue, 16 Jun 2020 09:40:49: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:40:49: #1 finished! INFO @ Tue, 16 Jun 2020 09:40:49: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:40:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:40:50: 36000000 INFO @ Tue, 16 Jun 2020 09:40:51: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:40:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:40:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX495078/SRX495078.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX495078/SRX495078.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX495078/SRX495078.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX495078/SRX495078.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:40:54: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:40:54: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:40:54: #1 total tags in treatment: 36622971 INFO @ Tue, 16 Jun 2020 09:40:54: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:40:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:40:54: #1 tags after filtering in treatment: 36622971 INFO @ Tue, 16 Jun 2020 09:40:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:40:54: #1 finished! INFO @ Tue, 16 Jun 2020 09:40:54: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:40:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:40:57: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:40:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:40:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX495078/SRX495078.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX495078/SRX495078.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX495078/SRX495078.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX495078/SRX495078.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling