Job ID = 6368352 SRX = SRX495032 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:18:06 prefetch.2.10.7: 1) Downloading 'SRR1198564'... 2020-06-16T00:18:06 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:21:52 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:21:52 prefetch.2.10.7: 1) 'SRR1198564' was downloaded successfully Read 28232537 spots for SRR1198564/SRR1198564.sra Written 28232537 spots for SRR1198564/SRR1198564.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:09 28232537 reads; of these: 28232537 (100.00%) were unpaired; of these: 1991836 (7.06%) aligned 0 times 22241658 (78.78%) aligned exactly 1 time 3999043 (14.16%) aligned >1 times 92.94% overall alignment rate Time searching: 00:06:09 Overall time: 00:06:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4083393 / 26240701 = 0.1556 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:35:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX495032/SRX495032.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX495032/SRX495032.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX495032/SRX495032.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX495032/SRX495032.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:35:41: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:35:41: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:35:46: 1000000 INFO @ Tue, 16 Jun 2020 09:35:52: 2000000 INFO @ Tue, 16 Jun 2020 09:35:57: 3000000 INFO @ Tue, 16 Jun 2020 09:36:03: 4000000 INFO @ Tue, 16 Jun 2020 09:36:08: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:36:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX495032/SRX495032.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX495032/SRX495032.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX495032/SRX495032.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX495032/SRX495032.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:36:11: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:36:11: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:36:14: 6000000 INFO @ Tue, 16 Jun 2020 09:36:17: 1000000 INFO @ Tue, 16 Jun 2020 09:36:20: 7000000 INFO @ Tue, 16 Jun 2020 09:36:23: 2000000 INFO @ Tue, 16 Jun 2020 09:36:27: 8000000 INFO @ Tue, 16 Jun 2020 09:36:29: 3000000 INFO @ Tue, 16 Jun 2020 09:36:33: 9000000 INFO @ Tue, 16 Jun 2020 09:36:35: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:36:39: 10000000 INFO @ Tue, 16 Jun 2020 09:36:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX495032/SRX495032.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX495032/SRX495032.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX495032/SRX495032.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX495032/SRX495032.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:36:41: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:36:41: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:36:41: 5000000 INFO @ Tue, 16 Jun 2020 09:36:45: 11000000 INFO @ Tue, 16 Jun 2020 09:36:47: 1000000 INFO @ Tue, 16 Jun 2020 09:36:48: 6000000 INFO @ Tue, 16 Jun 2020 09:36:51: 12000000 INFO @ Tue, 16 Jun 2020 09:36:53: 2000000 INFO @ Tue, 16 Jun 2020 09:36:54: 7000000 INFO @ Tue, 16 Jun 2020 09:36:58: 13000000 INFO @ Tue, 16 Jun 2020 09:37:00: 3000000 INFO @ Tue, 16 Jun 2020 09:37:00: 8000000 INFO @ Tue, 16 Jun 2020 09:37:04: 14000000 INFO @ Tue, 16 Jun 2020 09:37:06: 4000000 INFO @ Tue, 16 Jun 2020 09:37:07: 9000000 INFO @ Tue, 16 Jun 2020 09:37:10: 15000000 INFO @ Tue, 16 Jun 2020 09:37:12: 5000000 INFO @ Tue, 16 Jun 2020 09:37:13: 10000000 INFO @ Tue, 16 Jun 2020 09:37:16: 16000000 INFO @ Tue, 16 Jun 2020 09:37:19: 6000000 INFO @ Tue, 16 Jun 2020 09:37:19: 11000000 INFO @ Tue, 16 Jun 2020 09:37:23: 17000000 INFO @ Tue, 16 Jun 2020 09:37:25: 7000000 INFO @ Tue, 16 Jun 2020 09:37:26: 12000000 INFO @ Tue, 16 Jun 2020 09:37:29: 18000000 INFO @ Tue, 16 Jun 2020 09:37:31: 8000000 INFO @ Tue, 16 Jun 2020 09:37:32: 13000000 INFO @ Tue, 16 Jun 2020 09:37:35: 19000000 INFO @ Tue, 16 Jun 2020 09:37:38: 9000000 INFO @ Tue, 16 Jun 2020 09:37:38: 14000000 INFO @ Tue, 16 Jun 2020 09:37:42: 20000000 INFO @ Tue, 16 Jun 2020 09:37:44: 10000000 INFO @ Tue, 16 Jun 2020 09:37:45: 15000000 INFO @ Tue, 16 Jun 2020 09:37:48: 21000000 INFO @ Tue, 16 Jun 2020 09:37:50: 11000000 INFO @ Tue, 16 Jun 2020 09:37:51: 16000000 INFO @ Tue, 16 Jun 2020 09:37:54: 22000000 INFO @ Tue, 16 Jun 2020 09:37:56: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:37:56: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:37:56: #1 total tags in treatment: 22157308 INFO @ Tue, 16 Jun 2020 09:37:56: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:37:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:37:56: #1 tags after filtering in treatment: 22157308 INFO @ Tue, 16 Jun 2020 09:37:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:37:56: #1 finished! INFO @ Tue, 16 Jun 2020 09:37:56: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:37:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:37:57: 12000000 INFO @ Tue, 16 Jun 2020 09:37:57: 17000000 INFO @ Tue, 16 Jun 2020 09:37:57: #2 number of paired peaks: 74 WARNING @ Tue, 16 Jun 2020 09:37:57: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:37:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX495032/SRX495032.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX495032/SRX495032.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX495032/SRX495032.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX495032/SRX495032.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:38:03: 13000000 INFO @ Tue, 16 Jun 2020 09:38:03: 18000000 INFO @ Tue, 16 Jun 2020 09:38:09: 14000000 INFO @ Tue, 16 Jun 2020 09:38:10: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:38:16: 15000000 INFO @ Tue, 16 Jun 2020 09:38:16: 20000000 INFO @ Tue, 16 Jun 2020 09:38:22: 16000000 INFO @ Tue, 16 Jun 2020 09:38:22: 21000000 INFO @ Tue, 16 Jun 2020 09:38:28: 17000000 INFO @ Tue, 16 Jun 2020 09:38:29: 22000000 INFO @ Tue, 16 Jun 2020 09:38:30: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:38:30: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:38:30: #1 total tags in treatment: 22157308 INFO @ Tue, 16 Jun 2020 09:38:30: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:38:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:38:30: #1 tags after filtering in treatment: 22157308 INFO @ Tue, 16 Jun 2020 09:38:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:38:30: #1 finished! INFO @ Tue, 16 Jun 2020 09:38:30: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:38:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:38:32: #2 number of paired peaks: 74 WARNING @ Tue, 16 Jun 2020 09:38:32: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:38:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX495032/SRX495032.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX495032/SRX495032.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX495032/SRX495032.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX495032/SRX495032.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:38:34: 18000000 INFO @ Tue, 16 Jun 2020 09:38:40: 19000000 INFO @ Tue, 16 Jun 2020 09:38:46: 20000000 INFO @ Tue, 16 Jun 2020 09:38:52: 21000000 INFO @ Tue, 16 Jun 2020 09:38:58: 22000000 INFO @ Tue, 16 Jun 2020 09:38:59: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:38:59: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:38:59: #1 total tags in treatment: 22157308 INFO @ Tue, 16 Jun 2020 09:38:59: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:38:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:38:59: #1 tags after filtering in treatment: 22157308 INFO @ Tue, 16 Jun 2020 09:38:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:38:59: #1 finished! INFO @ Tue, 16 Jun 2020 09:38:59: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:38:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:39:01: #2 number of paired peaks: 74 WARNING @ Tue, 16 Jun 2020 09:39:01: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:39:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX495032/SRX495032.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX495032/SRX495032.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX495032/SRX495032.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX495032/SRX495032.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。