Job ID = 6507848 SRX = SRX494932 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-26T13:39:12 prefetch.2.10.7: 1) Downloading 'SRR1198464'... 2020-06-26T13:39:12 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T13:45:55 prefetch.2.10.7: HTTPS download succeed 2020-06-26T13:45:55 prefetch.2.10.7: 1) 'SRR1198464' was downloaded successfully Read 47950615 spots for SRR1198464/SRR1198464.sra Written 47950615 spots for SRR1198464/SRR1198464.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:46 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3138648 (6.55%) aligned 0 times 37485730 (78.18%) aligned exactly 1 time 7326237 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:12:46 Overall time: 00:12:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8188346 / 44811967 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 23:13:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494932/SRX494932.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494932/SRX494932.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494932/SRX494932.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494932/SRX494932.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 23:13:33: #1 read tag files... INFO @ Fri, 26 Jun 2020 23:13:33: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 23:13:39: 1000000 INFO @ Fri, 26 Jun 2020 23:13:45: 2000000 INFO @ Fri, 26 Jun 2020 23:13:51: 3000000 INFO @ Fri, 26 Jun 2020 23:13:57: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 23:14:03: 5000000 INFO @ Fri, 26 Jun 2020 23:14:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494932/SRX494932.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494932/SRX494932.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494932/SRX494932.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494932/SRX494932.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 23:14:03: #1 read tag files... INFO @ Fri, 26 Jun 2020 23:14:03: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 23:14:09: 6000000 INFO @ Fri, 26 Jun 2020 23:14:10: 1000000 INFO @ Fri, 26 Jun 2020 23:14:16: 7000000 INFO @ Fri, 26 Jun 2020 23:14:16: 2000000 INFO @ Fri, 26 Jun 2020 23:14:22: 8000000 INFO @ Fri, 26 Jun 2020 23:14:23: 3000000 INFO @ Fri, 26 Jun 2020 23:14:29: 9000000 INFO @ Fri, 26 Jun 2020 23:14:29: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 23:14:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494932/SRX494932.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494932/SRX494932.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494932/SRX494932.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494932/SRX494932.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 23:14:33: #1 read tag files... INFO @ Fri, 26 Jun 2020 23:14:33: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 23:14:35: 10000000 INFO @ Fri, 26 Jun 2020 23:14:36: 5000000 INFO @ Fri, 26 Jun 2020 23:14:40: 1000000 INFO @ Fri, 26 Jun 2020 23:14:42: 11000000 INFO @ Fri, 26 Jun 2020 23:14:42: 6000000 INFO @ Fri, 26 Jun 2020 23:14:47: 2000000 INFO @ Fri, 26 Jun 2020 23:14:49: 12000000 INFO @ Fri, 26 Jun 2020 23:14:49: 7000000 INFO @ Fri, 26 Jun 2020 23:14:53: 3000000 INFO @ Fri, 26 Jun 2020 23:14:55: 13000000 INFO @ Fri, 26 Jun 2020 23:14:55: 8000000 INFO @ Fri, 26 Jun 2020 23:15:00: 4000000 INFO @ Fri, 26 Jun 2020 23:15:02: 14000000 INFO @ Fri, 26 Jun 2020 23:15:02: 9000000 INFO @ Fri, 26 Jun 2020 23:15:06: 5000000 INFO @ Fri, 26 Jun 2020 23:15:08: 15000000 INFO @ Fri, 26 Jun 2020 23:15:09: 10000000 INFO @ Fri, 26 Jun 2020 23:15:13: 6000000 INFO @ Fri, 26 Jun 2020 23:15:15: 16000000 INFO @ Fri, 26 Jun 2020 23:15:15: 11000000 INFO @ Fri, 26 Jun 2020 23:15:19: 7000000 INFO @ Fri, 26 Jun 2020 23:15:22: 17000000 INFO @ Fri, 26 Jun 2020 23:15:22: 12000000 INFO @ Fri, 26 Jun 2020 23:15:26: 8000000 INFO @ Fri, 26 Jun 2020 23:15:28: 13000000 INFO @ Fri, 26 Jun 2020 23:15:28: 18000000 INFO @ Fri, 26 Jun 2020 23:15:32: 9000000 INFO @ Fri, 26 Jun 2020 23:15:35: 14000000 INFO @ Fri, 26 Jun 2020 23:15:35: 19000000 INFO @ Fri, 26 Jun 2020 23:15:39: 10000000 INFO @ Fri, 26 Jun 2020 23:15:41: 15000000 INFO @ Fri, 26 Jun 2020 23:15:41: 20000000 INFO @ Fri, 26 Jun 2020 23:15:45: 11000000 INFO @ Fri, 26 Jun 2020 23:15:47: 16000000 INFO @ Fri, 26 Jun 2020 23:15:48: 21000000 INFO @ Fri, 26 Jun 2020 23:15:52: 12000000 INFO @ Fri, 26 Jun 2020 23:15:54: 17000000 INFO @ Fri, 26 Jun 2020 23:15:54: 22000000 INFO @ Fri, 26 Jun 2020 23:15:58: 13000000 INFO @ Fri, 26 Jun 2020 23:16:00: 18000000 INFO @ Fri, 26 Jun 2020 23:16:01: 23000000 INFO @ Fri, 26 Jun 2020 23:16:05: 14000000 INFO @ Fri, 26 Jun 2020 23:16:07: 19000000 INFO @ Fri, 26 Jun 2020 23:16:07: 24000000 INFO @ Fri, 26 Jun 2020 23:16:11: 15000000 INFO @ Fri, 26 Jun 2020 23:16:13: 20000000 INFO @ Fri, 26 Jun 2020 23:16:14: 25000000 INFO @ Fri, 26 Jun 2020 23:16:17: 16000000 INFO @ Fri, 26 Jun 2020 23:16:19: 21000000 INFO @ Fri, 26 Jun 2020 23:16:20: 26000000 INFO @ Fri, 26 Jun 2020 23:16:24: 17000000 INFO @ Fri, 26 Jun 2020 23:16:26: 22000000 INFO @ Fri, 26 Jun 2020 23:16:27: 27000000 INFO @ Fri, 26 Jun 2020 23:16:30: 18000000 INFO @ Fri, 26 Jun 2020 23:16:32: 23000000 INFO @ Fri, 26 Jun 2020 23:16:33: 28000000 INFO @ Fri, 26 Jun 2020 23:16:36: 19000000 INFO @ Fri, 26 Jun 2020 23:16:39: 24000000 INFO @ Fri, 26 Jun 2020 23:16:39: 29000000 INFO @ Fri, 26 Jun 2020 23:16:43: 20000000 INFO @ Fri, 26 Jun 2020 23:16:45: 25000000 INFO @ Fri, 26 Jun 2020 23:16:46: 30000000 INFO @ Fri, 26 Jun 2020 23:16:49: 21000000 INFO @ Fri, 26 Jun 2020 23:16:51: 26000000 INFO @ Fri, 26 Jun 2020 23:16:52: 31000000 INFO @ Fri, 26 Jun 2020 23:16:55: 22000000 INFO @ Fri, 26 Jun 2020 23:16:58: 27000000 INFO @ Fri, 26 Jun 2020 23:16:59: 32000000 INFO @ Fri, 26 Jun 2020 23:17:02: 23000000 INFO @ Fri, 26 Jun 2020 23:17:04: 28000000 INFO @ Fri, 26 Jun 2020 23:17:05: 33000000 INFO @ Fri, 26 Jun 2020 23:17:08: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 23:17:10: 29000000 INFO @ Fri, 26 Jun 2020 23:17:11: 34000000 INFO @ Fri, 26 Jun 2020 23:17:15: 25000000 INFO @ Fri, 26 Jun 2020 23:17:17: 30000000 INFO @ Fri, 26 Jun 2020 23:17:18: 35000000 INFO @ Fri, 26 Jun 2020 23:17:21: 26000000 INFO @ Fri, 26 Jun 2020 23:17:23: 31000000 INFO @ Fri, 26 Jun 2020 23:17:24: 36000000 INFO @ Fri, 26 Jun 2020 23:17:28: 27000000 INFO @ Fri, 26 Jun 2020 23:17:28: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 23:17:28: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 23:17:28: #1 total tags in treatment: 36623621 INFO @ Fri, 26 Jun 2020 23:17:28: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 23:17:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 23:17:29: #1 tags after filtering in treatment: 36623621 INFO @ Fri, 26 Jun 2020 23:17:29: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 23:17:29: #1 finished! INFO @ Fri, 26 Jun 2020 23:17:29: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 23:17:29: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 23:17:29: 32000000 INFO @ Fri, 26 Jun 2020 23:17:31: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 23:17:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 23:17:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494932/SRX494932.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494932/SRX494932.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494932/SRX494932.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494932/SRX494932.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 23:17:34: 28000000 INFO @ Fri, 26 Jun 2020 23:17:35: 33000000 INFO @ Fri, 26 Jun 2020 23:17:40: 29000000 INFO @ Fri, 26 Jun 2020 23:17:42: 34000000 INFO @ Fri, 26 Jun 2020 23:17:46: 30000000 INFO @ Fri, 26 Jun 2020 23:17:48: 35000000 INFO @ Fri, 26 Jun 2020 23:17:53: 31000000 INFO @ Fri, 26 Jun 2020 23:17:54: 36000000 INFO @ Fri, 26 Jun 2020 23:17:58: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 23:17:58: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 23:17:58: #1 total tags in treatment: 36623621 INFO @ Fri, 26 Jun 2020 23:17:58: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 23:17:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 23:17:59: 32000000 INFO @ Fri, 26 Jun 2020 23:17:59: #1 tags after filtering in treatment: 36623621 INFO @ Fri, 26 Jun 2020 23:17:59: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 23:17:59: #1 finished! INFO @ Fri, 26 Jun 2020 23:17:59: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 23:17:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 23:18:01: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 23:18:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 23:18:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494932/SRX494932.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494932/SRX494932.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494932/SRX494932.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494932/SRX494932.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 23:18:05: 33000000 INFO @ Fri, 26 Jun 2020 23:18:11: 34000000 INFO @ Fri, 26 Jun 2020 23:18:17: 35000000 BigWig に変換しました。 INFO @ Fri, 26 Jun 2020 23:18:23: 36000000 INFO @ Fri, 26 Jun 2020 23:18:26: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 23:18:26: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 23:18:26: #1 total tags in treatment: 36623621 INFO @ Fri, 26 Jun 2020 23:18:26: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 23:18:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 23:18:27: #1 tags after filtering in treatment: 36623621 INFO @ Fri, 26 Jun 2020 23:18:27: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 23:18:27: #1 finished! INFO @ Fri, 26 Jun 2020 23:18:27: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 23:18:27: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 23:18:29: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 23:18:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 23:18:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494932/SRX494932.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494932/SRX494932.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494932/SRX494932.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494932/SRX494932.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling