Job ID = 6507843 SRX = SRX494928 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-26T13:06:47 prefetch.2.10.7: 1) Downloading 'SRR1198460'... 2020-06-26T13:06:47 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T13:10:59 prefetch.2.10.7: HTTPS download succeed 2020-06-26T13:10:59 prefetch.2.10.7: 1) 'SRR1198460' was downloaded successfully Read 47950615 spots for SRR1198460/SRR1198460.sra Written 47950615 spots for SRR1198460/SRR1198460.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:28 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3138713 (6.55%) aligned 0 times 37485864 (78.18%) aligned exactly 1 time 7326038 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:10:29 Overall time: 00:10:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8187938 / 44811902 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 22:34:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494928/SRX494928.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494928/SRX494928.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494928/SRX494928.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494928/SRX494928.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 22:34:10: #1 read tag files... INFO @ Fri, 26 Jun 2020 22:34:10: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 22:34:17: 1000000 INFO @ Fri, 26 Jun 2020 22:34:23: 2000000 INFO @ Fri, 26 Jun 2020 22:34:29: 3000000 INFO @ Fri, 26 Jun 2020 22:34:35: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 22:34:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494928/SRX494928.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494928/SRX494928.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494928/SRX494928.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494928/SRX494928.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 22:34:40: #1 read tag files... INFO @ Fri, 26 Jun 2020 22:34:40: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 22:34:41: 5000000 INFO @ Fri, 26 Jun 2020 22:34:46: 1000000 INFO @ Fri, 26 Jun 2020 22:34:47: 6000000 INFO @ Fri, 26 Jun 2020 22:34:52: 2000000 INFO @ Fri, 26 Jun 2020 22:34:54: 7000000 INFO @ Fri, 26 Jun 2020 22:34:58: 3000000 INFO @ Fri, 26 Jun 2020 22:35:00: 8000000 INFO @ Fri, 26 Jun 2020 22:35:05: 4000000 INFO @ Fri, 26 Jun 2020 22:35:06: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 22:35:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494928/SRX494928.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494928/SRX494928.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494928/SRX494928.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494928/SRX494928.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 22:35:10: #1 read tag files... INFO @ Fri, 26 Jun 2020 22:35:10: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 22:35:11: 5000000 INFO @ Fri, 26 Jun 2020 22:35:12: 10000000 INFO @ Fri, 26 Jun 2020 22:35:17: 1000000 INFO @ Fri, 26 Jun 2020 22:35:17: 6000000 INFO @ Fri, 26 Jun 2020 22:35:19: 11000000 INFO @ Fri, 26 Jun 2020 22:35:23: 2000000 INFO @ Fri, 26 Jun 2020 22:35:23: 7000000 INFO @ Fri, 26 Jun 2020 22:35:25: 12000000 INFO @ Fri, 26 Jun 2020 22:35:29: 3000000 INFO @ Fri, 26 Jun 2020 22:35:30: 8000000 INFO @ Fri, 26 Jun 2020 22:35:32: 13000000 INFO @ Fri, 26 Jun 2020 22:35:35: 4000000 INFO @ Fri, 26 Jun 2020 22:35:36: 9000000 INFO @ Fri, 26 Jun 2020 22:35:38: 14000000 INFO @ Fri, 26 Jun 2020 22:35:41: 5000000 INFO @ Fri, 26 Jun 2020 22:35:42: 10000000 INFO @ Fri, 26 Jun 2020 22:35:44: 15000000 INFO @ Fri, 26 Jun 2020 22:35:47: 6000000 INFO @ Fri, 26 Jun 2020 22:35:48: 11000000 INFO @ Fri, 26 Jun 2020 22:35:50: 16000000 INFO @ Fri, 26 Jun 2020 22:35:54: 7000000 INFO @ Fri, 26 Jun 2020 22:35:54: 12000000 INFO @ Fri, 26 Jun 2020 22:35:56: 17000000 INFO @ Fri, 26 Jun 2020 22:36:00: 13000000 INFO @ Fri, 26 Jun 2020 22:36:01: 8000000 INFO @ Fri, 26 Jun 2020 22:36:03: 18000000 INFO @ Fri, 26 Jun 2020 22:36:07: 9000000 INFO @ Fri, 26 Jun 2020 22:36:07: 14000000 INFO @ Fri, 26 Jun 2020 22:36:09: 19000000 INFO @ Fri, 26 Jun 2020 22:36:13: 10000000 INFO @ Fri, 26 Jun 2020 22:36:13: 15000000 INFO @ Fri, 26 Jun 2020 22:36:15: 20000000 INFO @ Fri, 26 Jun 2020 22:36:19: 11000000 INFO @ Fri, 26 Jun 2020 22:36:19: 16000000 INFO @ Fri, 26 Jun 2020 22:36:21: 21000000 INFO @ Fri, 26 Jun 2020 22:36:25: 17000000 INFO @ Fri, 26 Jun 2020 22:36:25: 12000000 INFO @ Fri, 26 Jun 2020 22:36:27: 22000000 INFO @ Fri, 26 Jun 2020 22:36:32: 18000000 INFO @ Fri, 26 Jun 2020 22:36:32: 13000000 INFO @ Fri, 26 Jun 2020 22:36:33: 23000000 INFO @ Fri, 26 Jun 2020 22:36:38: 19000000 INFO @ Fri, 26 Jun 2020 22:36:38: 14000000 INFO @ Fri, 26 Jun 2020 22:36:39: 24000000 INFO @ Fri, 26 Jun 2020 22:36:44: 20000000 INFO @ Fri, 26 Jun 2020 22:36:44: 15000000 INFO @ Fri, 26 Jun 2020 22:36:46: 25000000 INFO @ Fri, 26 Jun 2020 22:36:50: 21000000 INFO @ Fri, 26 Jun 2020 22:36:50: 16000000 INFO @ Fri, 26 Jun 2020 22:36:52: 26000000 INFO @ Fri, 26 Jun 2020 22:36:56: 22000000 INFO @ Fri, 26 Jun 2020 22:36:56: 17000000 INFO @ Fri, 26 Jun 2020 22:36:58: 27000000 INFO @ Fri, 26 Jun 2020 22:37:02: 23000000 INFO @ Fri, 26 Jun 2020 22:37:02: 18000000 INFO @ Fri, 26 Jun 2020 22:37:04: 28000000 INFO @ Fri, 26 Jun 2020 22:37:08: 24000000 INFO @ Fri, 26 Jun 2020 22:37:08: 19000000 INFO @ Fri, 26 Jun 2020 22:37:10: 29000000 INFO @ Fri, 26 Jun 2020 22:37:14: 25000000 INFO @ Fri, 26 Jun 2020 22:37:14: 20000000 INFO @ Fri, 26 Jun 2020 22:37:16: 30000000 INFO @ Fri, 26 Jun 2020 22:37:20: 21000000 INFO @ Fri, 26 Jun 2020 22:37:20: 26000000 INFO @ Fri, 26 Jun 2020 22:37:22: 31000000 INFO @ Fri, 26 Jun 2020 22:37:26: 22000000 INFO @ Fri, 26 Jun 2020 22:37:26: 27000000 INFO @ Fri, 26 Jun 2020 22:37:28: 32000000 INFO @ Fri, 26 Jun 2020 22:37:32: 23000000 INFO @ Fri, 26 Jun 2020 22:37:32: 28000000 INFO @ Fri, 26 Jun 2020 22:37:34: 33000000 INFO @ Fri, 26 Jun 2020 22:37:38: 24000000 INFO @ Fri, 26 Jun 2020 22:37:39: 29000000 INFO @ Fri, 26 Jun 2020 22:37:40: 34000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 22:37:45: 25000000 INFO @ Fri, 26 Jun 2020 22:37:45: 30000000 INFO @ Fri, 26 Jun 2020 22:37:46: 35000000 INFO @ Fri, 26 Jun 2020 22:37:51: 31000000 INFO @ Fri, 26 Jun 2020 22:37:51: 26000000 INFO @ Fri, 26 Jun 2020 22:37:53: 36000000 INFO @ Fri, 26 Jun 2020 22:37:57: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 22:37:57: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 22:37:57: #1 total tags in treatment: 36623964 INFO @ Fri, 26 Jun 2020 22:37:57: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 22:37:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 22:37:57: 32000000 INFO @ Fri, 26 Jun 2020 22:37:57: 27000000 INFO @ Fri, 26 Jun 2020 22:37:57: #1 tags after filtering in treatment: 36623964 INFO @ Fri, 26 Jun 2020 22:37:57: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 22:37:57: #1 finished! INFO @ Fri, 26 Jun 2020 22:37:57: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 22:37:57: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 22:37:59: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 22:37:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 22:37:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494928/SRX494928.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494928/SRX494928.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494928/SRX494928.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494928/SRX494928.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 22:38:03: 33000000 INFO @ Fri, 26 Jun 2020 22:38:03: 28000000 INFO @ Fri, 26 Jun 2020 22:38:09: 34000000 INFO @ Fri, 26 Jun 2020 22:38:09: 29000000 INFO @ Fri, 26 Jun 2020 22:38:16: 35000000 INFO @ Fri, 26 Jun 2020 22:38:16: 30000000 INFO @ Fri, 26 Jun 2020 22:38:22: 36000000 INFO @ Fri, 26 Jun 2020 22:38:22: 31000000 INFO @ Fri, 26 Jun 2020 22:38:26: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 22:38:26: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 22:38:26: #1 total tags in treatment: 36623964 INFO @ Fri, 26 Jun 2020 22:38:26: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 22:38:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 22:38:26: #1 tags after filtering in treatment: 36623964 INFO @ Fri, 26 Jun 2020 22:38:26: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 22:38:26: #1 finished! INFO @ Fri, 26 Jun 2020 22:38:26: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 22:38:26: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 22:38:28: 32000000 INFO @ Fri, 26 Jun 2020 22:38:29: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 22:38:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 22:38:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494928/SRX494928.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494928/SRX494928.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494928/SRX494928.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494928/SRX494928.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 22:38:34: 33000000 INFO @ Fri, 26 Jun 2020 22:38:40: 34000000 INFO @ Fri, 26 Jun 2020 22:38:46: 35000000 BigWig に変換しました。 INFO @ Fri, 26 Jun 2020 22:38:51: 36000000 INFO @ Fri, 26 Jun 2020 22:38:55: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 22:38:55: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 22:38:55: #1 total tags in treatment: 36623964 INFO @ Fri, 26 Jun 2020 22:38:55: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 22:38:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 22:38:56: #1 tags after filtering in treatment: 36623964 INFO @ Fri, 26 Jun 2020 22:38:56: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 22:38:56: #1 finished! INFO @ Fri, 26 Jun 2020 22:38:56: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 22:38:56: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 22:38:58: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 22:38:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 22:38:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494928/SRX494928.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494928/SRX494928.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494928/SRX494928.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494928/SRX494928.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling