Job ID = 6368228 SRX = SRX494911 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:54:33 prefetch.2.10.7: 1) Downloading 'SRR1198443'... 2020-06-15T23:54:33 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:59:05 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:59:05 prefetch.2.10.7: 1) 'SRR1198443' was downloaded successfully Read 47950615 spots for SRR1198443/SRR1198443.sra Written 47950615 spots for SRR1198443/SRR1198443.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:49 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3138687 (6.55%) aligned 0 times 37485784 (78.18%) aligned exactly 1 time 7326144 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:09:49 Overall time: 00:09:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8188742 / 44811928 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:24:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494911/SRX494911.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494911/SRX494911.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494911/SRX494911.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494911/SRX494911.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:24:23: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:24:23: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:24:30: 1000000 INFO @ Tue, 16 Jun 2020 09:24:37: 2000000 INFO @ Tue, 16 Jun 2020 09:24:45: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:24:52: 4000000 INFO @ Tue, 16 Jun 2020 09:24:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494911/SRX494911.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494911/SRX494911.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494911/SRX494911.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494911/SRX494911.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:24:53: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:24:53: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:24:59: 5000000 INFO @ Tue, 16 Jun 2020 09:25:00: 1000000 INFO @ Tue, 16 Jun 2020 09:25:06: 6000000 INFO @ Tue, 16 Jun 2020 09:25:07: 2000000 INFO @ Tue, 16 Jun 2020 09:25:13: 7000000 INFO @ Tue, 16 Jun 2020 09:25:14: 3000000 INFO @ Tue, 16 Jun 2020 09:25:20: 8000000 INFO @ Tue, 16 Jun 2020 09:25:20: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:25:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494911/SRX494911.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494911/SRX494911.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494911/SRX494911.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494911/SRX494911.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:25:23: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:25:23: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:25:27: 9000000 INFO @ Tue, 16 Jun 2020 09:25:27: 5000000 INFO @ Tue, 16 Jun 2020 09:25:29: 1000000 INFO @ Tue, 16 Jun 2020 09:25:34: 10000000 INFO @ Tue, 16 Jun 2020 09:25:34: 6000000 INFO @ Tue, 16 Jun 2020 09:25:35: 2000000 INFO @ Tue, 16 Jun 2020 09:25:41: 11000000 INFO @ Tue, 16 Jun 2020 09:25:41: 3000000 INFO @ Tue, 16 Jun 2020 09:25:42: 7000000 INFO @ Tue, 16 Jun 2020 09:25:47: 4000000 INFO @ Tue, 16 Jun 2020 09:25:48: 12000000 INFO @ Tue, 16 Jun 2020 09:25:49: 8000000 INFO @ Tue, 16 Jun 2020 09:25:53: 5000000 INFO @ Tue, 16 Jun 2020 09:25:55: 13000000 INFO @ Tue, 16 Jun 2020 09:25:56: 9000000 INFO @ Tue, 16 Jun 2020 09:25:59: 6000000 INFO @ Tue, 16 Jun 2020 09:26:02: 14000000 INFO @ Tue, 16 Jun 2020 09:26:03: 10000000 INFO @ Tue, 16 Jun 2020 09:26:05: 7000000 INFO @ Tue, 16 Jun 2020 09:26:09: 15000000 INFO @ Tue, 16 Jun 2020 09:26:10: 11000000 INFO @ Tue, 16 Jun 2020 09:26:11: 8000000 INFO @ Tue, 16 Jun 2020 09:26:16: 16000000 INFO @ Tue, 16 Jun 2020 09:26:16: 12000000 INFO @ Tue, 16 Jun 2020 09:26:17: 9000000 INFO @ Tue, 16 Jun 2020 09:26:22: 17000000 INFO @ Tue, 16 Jun 2020 09:26:23: 13000000 INFO @ Tue, 16 Jun 2020 09:26:23: 10000000 INFO @ Tue, 16 Jun 2020 09:26:29: 18000000 INFO @ Tue, 16 Jun 2020 09:26:29: 11000000 INFO @ Tue, 16 Jun 2020 09:26:30: 14000000 INFO @ Tue, 16 Jun 2020 09:26:35: 12000000 INFO @ Tue, 16 Jun 2020 09:26:35: 19000000 INFO @ Tue, 16 Jun 2020 09:26:36: 15000000 INFO @ Tue, 16 Jun 2020 09:26:41: 13000000 INFO @ Tue, 16 Jun 2020 09:26:42: 20000000 INFO @ Tue, 16 Jun 2020 09:26:42: 16000000 INFO @ Tue, 16 Jun 2020 09:26:47: 14000000 INFO @ Tue, 16 Jun 2020 09:26:48: 21000000 INFO @ Tue, 16 Jun 2020 09:26:49: 17000000 INFO @ Tue, 16 Jun 2020 09:26:53: 15000000 INFO @ Tue, 16 Jun 2020 09:26:54: 22000000 INFO @ Tue, 16 Jun 2020 09:26:55: 18000000 INFO @ Tue, 16 Jun 2020 09:26:59: 16000000 INFO @ Tue, 16 Jun 2020 09:27:01: 23000000 INFO @ Tue, 16 Jun 2020 09:27:01: 19000000 INFO @ Tue, 16 Jun 2020 09:27:05: 17000000 INFO @ Tue, 16 Jun 2020 09:27:07: 24000000 INFO @ Tue, 16 Jun 2020 09:27:08: 20000000 INFO @ Tue, 16 Jun 2020 09:27:11: 18000000 INFO @ Tue, 16 Jun 2020 09:27:13: 25000000 INFO @ Tue, 16 Jun 2020 09:27:14: 21000000 INFO @ Tue, 16 Jun 2020 09:27:17: 19000000 INFO @ Tue, 16 Jun 2020 09:27:20: 26000000 INFO @ Tue, 16 Jun 2020 09:27:20: 22000000 INFO @ Tue, 16 Jun 2020 09:27:23: 20000000 INFO @ Tue, 16 Jun 2020 09:27:26: 27000000 INFO @ Tue, 16 Jun 2020 09:27:27: 23000000 INFO @ Tue, 16 Jun 2020 09:27:29: 21000000 INFO @ Tue, 16 Jun 2020 09:27:32: 28000000 INFO @ Tue, 16 Jun 2020 09:27:33: 24000000 INFO @ Tue, 16 Jun 2020 09:27:35: 22000000 INFO @ Tue, 16 Jun 2020 09:27:39: 29000000 INFO @ Tue, 16 Jun 2020 09:27:40: 25000000 INFO @ Tue, 16 Jun 2020 09:27:42: 23000000 INFO @ Tue, 16 Jun 2020 09:27:45: 30000000 INFO @ Tue, 16 Jun 2020 09:27:47: 26000000 INFO @ Tue, 16 Jun 2020 09:27:48: 24000000 INFO @ Tue, 16 Jun 2020 09:27:51: 31000000 INFO @ Tue, 16 Jun 2020 09:27:54: 27000000 INFO @ Tue, 16 Jun 2020 09:27:54: 25000000 INFO @ Tue, 16 Jun 2020 09:27:57: 32000000 INFO @ Tue, 16 Jun 2020 09:28:00: 26000000 INFO @ Tue, 16 Jun 2020 09:28:00: 28000000 INFO @ Tue, 16 Jun 2020 09:28:04: 33000000 INFO @ Tue, 16 Jun 2020 09:28:06: 27000000 INFO @ Tue, 16 Jun 2020 09:28:07: 29000000 INFO @ Tue, 16 Jun 2020 09:28:10: 34000000 INFO @ Tue, 16 Jun 2020 09:28:12: 28000000 INFO @ Tue, 16 Jun 2020 09:28:13: 30000000 INFO @ Tue, 16 Jun 2020 09:28:17: 35000000 INFO @ Tue, 16 Jun 2020 09:28:18: 29000000 INFO @ Tue, 16 Jun 2020 09:28:20: 31000000 INFO @ Tue, 16 Jun 2020 09:28:24: 36000000 INFO @ Tue, 16 Jun 2020 09:28:24: 30000000 INFO @ Tue, 16 Jun 2020 09:28:26: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:28:28: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:28:28: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:28:28: #1 total tags in treatment: 36623186 INFO @ Tue, 16 Jun 2020 09:28:28: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:28:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:28:29: #1 tags after filtering in treatment: 36623186 INFO @ Tue, 16 Jun 2020 09:28:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:28:29: #1 finished! INFO @ Tue, 16 Jun 2020 09:28:29: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:28:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:28:30: 31000000 INFO @ Tue, 16 Jun 2020 09:28:31: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:28:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:28:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494911/SRX494911.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494911/SRX494911.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494911/SRX494911.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494911/SRX494911.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:28:32: 33000000 INFO @ Tue, 16 Jun 2020 09:28:36: 32000000 INFO @ Tue, 16 Jun 2020 09:28:38: 34000000 INFO @ Tue, 16 Jun 2020 09:28:42: 33000000 INFO @ Tue, 16 Jun 2020 09:28:44: 35000000 INFO @ Tue, 16 Jun 2020 09:28:48: 34000000 INFO @ Tue, 16 Jun 2020 09:28:50: 36000000 INFO @ Tue, 16 Jun 2020 09:28:54: 35000000 INFO @ Tue, 16 Jun 2020 09:28:54: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:28:54: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:28:54: #1 total tags in treatment: 36623186 INFO @ Tue, 16 Jun 2020 09:28:54: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:28:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:28:55: #1 tags after filtering in treatment: 36623186 INFO @ Tue, 16 Jun 2020 09:28:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:28:55: #1 finished! INFO @ Tue, 16 Jun 2020 09:28:55: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:28:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:28:57: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:28:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:28:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494911/SRX494911.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494911/SRX494911.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494911/SRX494911.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494911/SRX494911.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:29:00: 36000000 INFO @ Tue, 16 Jun 2020 09:29:04: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:29:04: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:29:04: #1 total tags in treatment: 36623186 INFO @ Tue, 16 Jun 2020 09:29:04: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:29:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:29:04: #1 tags after filtering in treatment: 36623186 INFO @ Tue, 16 Jun 2020 09:29:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:29:04: #1 finished! INFO @ Tue, 16 Jun 2020 09:29:04: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:29:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:29:07: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:29:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:29:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494911/SRX494911.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494911/SRX494911.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494911/SRX494911.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494911/SRX494911.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。