Job ID = 6368224 SRX = SRX494907 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:59:20 prefetch.2.10.7: 1) Downloading 'SRR1198439'... 2020-06-15T23:59:20 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:03:40 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:03:40 prefetch.2.10.7: 1) 'SRR1198439' was downloaded successfully Read 47950615 spots for SRR1198439/SRR1198439.sra Written 47950615 spots for SRR1198439/SRR1198439.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:27 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3138677 (6.55%) aligned 0 times 37485800 (78.18%) aligned exactly 1 time 7326138 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:10:27 Overall time: 00:10:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8188885 / 44811938 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:26:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494907/SRX494907.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494907/SRX494907.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494907/SRX494907.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494907/SRX494907.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:26:55: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:26:55: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:27:01: 1000000 INFO @ Tue, 16 Jun 2020 09:27:07: 2000000 INFO @ Tue, 16 Jun 2020 09:27:13: 3000000 INFO @ Tue, 16 Jun 2020 09:27:19: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:27:25: 5000000 INFO @ Tue, 16 Jun 2020 09:27:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494907/SRX494907.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494907/SRX494907.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494907/SRX494907.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494907/SRX494907.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:27:25: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:27:25: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:27:31: 6000000 INFO @ Tue, 16 Jun 2020 09:27:32: 1000000 INFO @ Tue, 16 Jun 2020 09:27:38: 7000000 INFO @ Tue, 16 Jun 2020 09:27:40: 2000000 INFO @ Tue, 16 Jun 2020 09:27:45: 8000000 INFO @ Tue, 16 Jun 2020 09:27:47: 3000000 INFO @ Tue, 16 Jun 2020 09:27:52: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:27:54: 4000000 INFO @ Tue, 16 Jun 2020 09:27:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494907/SRX494907.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494907/SRX494907.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494907/SRX494907.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494907/SRX494907.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:27:55: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:27:55: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:27:59: 10000000 INFO @ Tue, 16 Jun 2020 09:28:01: 5000000 INFO @ Tue, 16 Jun 2020 09:28:03: 1000000 INFO @ Tue, 16 Jun 2020 09:28:05: 11000000 INFO @ Tue, 16 Jun 2020 09:28:09: 6000000 INFO @ Tue, 16 Jun 2020 09:28:11: 2000000 INFO @ Tue, 16 Jun 2020 09:28:12: 12000000 INFO @ Tue, 16 Jun 2020 09:28:17: 7000000 INFO @ Tue, 16 Jun 2020 09:28:18: 3000000 INFO @ Tue, 16 Jun 2020 09:28:19: 13000000 INFO @ Tue, 16 Jun 2020 09:28:24: 8000000 INFO @ Tue, 16 Jun 2020 09:28:25: 14000000 INFO @ Tue, 16 Jun 2020 09:28:26: 4000000 INFO @ Tue, 16 Jun 2020 09:28:32: 15000000 INFO @ Tue, 16 Jun 2020 09:28:32: 9000000 INFO @ Tue, 16 Jun 2020 09:28:33: 5000000 INFO @ Tue, 16 Jun 2020 09:28:38: 16000000 INFO @ Tue, 16 Jun 2020 09:28:39: 10000000 INFO @ Tue, 16 Jun 2020 09:28:40: 6000000 INFO @ Tue, 16 Jun 2020 09:28:45: 17000000 INFO @ Tue, 16 Jun 2020 09:28:46: 11000000 INFO @ Tue, 16 Jun 2020 09:28:48: 7000000 INFO @ Tue, 16 Jun 2020 09:28:52: 18000000 INFO @ Tue, 16 Jun 2020 09:28:54: 12000000 INFO @ Tue, 16 Jun 2020 09:28:55: 8000000 INFO @ Tue, 16 Jun 2020 09:28:58: 19000000 INFO @ Tue, 16 Jun 2020 09:29:01: 13000000 INFO @ Tue, 16 Jun 2020 09:29:02: 9000000 INFO @ Tue, 16 Jun 2020 09:29:05: 20000000 INFO @ Tue, 16 Jun 2020 09:29:08: 14000000 INFO @ Tue, 16 Jun 2020 09:29:09: 10000000 INFO @ Tue, 16 Jun 2020 09:29:11: 21000000 INFO @ Tue, 16 Jun 2020 09:29:15: 15000000 INFO @ Tue, 16 Jun 2020 09:29:16: 11000000 INFO @ Tue, 16 Jun 2020 09:29:18: 22000000 INFO @ Tue, 16 Jun 2020 09:29:22: 16000000 INFO @ Tue, 16 Jun 2020 09:29:23: 12000000 INFO @ Tue, 16 Jun 2020 09:29:24: 23000000 INFO @ Tue, 16 Jun 2020 09:29:29: 17000000 INFO @ Tue, 16 Jun 2020 09:29:29: 13000000 INFO @ Tue, 16 Jun 2020 09:29:31: 24000000 INFO @ Tue, 16 Jun 2020 09:29:36: 14000000 INFO @ Tue, 16 Jun 2020 09:29:36: 18000000 INFO @ Tue, 16 Jun 2020 09:29:38: 25000000 INFO @ Tue, 16 Jun 2020 09:29:43: 19000000 INFO @ Tue, 16 Jun 2020 09:29:43: 15000000 INFO @ Tue, 16 Jun 2020 09:29:44: 26000000 INFO @ Tue, 16 Jun 2020 09:29:50: 16000000 INFO @ Tue, 16 Jun 2020 09:29:50: 20000000 INFO @ Tue, 16 Jun 2020 09:29:51: 27000000 INFO @ Tue, 16 Jun 2020 09:29:57: 17000000 INFO @ Tue, 16 Jun 2020 09:29:57: 21000000 INFO @ Tue, 16 Jun 2020 09:29:58: 28000000 INFO @ Tue, 16 Jun 2020 09:30:04: 18000000 INFO @ Tue, 16 Jun 2020 09:30:04: 22000000 INFO @ Tue, 16 Jun 2020 09:30:05: 29000000 INFO @ Tue, 16 Jun 2020 09:30:10: 19000000 INFO @ Tue, 16 Jun 2020 09:30:11: 30000000 INFO @ Tue, 16 Jun 2020 09:30:11: 23000000 INFO @ Tue, 16 Jun 2020 09:30:17: 20000000 INFO @ Tue, 16 Jun 2020 09:30:18: 31000000 INFO @ Tue, 16 Jun 2020 09:30:19: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:30:24: 21000000 INFO @ Tue, 16 Jun 2020 09:30:25: 32000000 INFO @ Tue, 16 Jun 2020 09:30:26: 25000000 INFO @ Tue, 16 Jun 2020 09:30:31: 22000000 INFO @ Tue, 16 Jun 2020 09:30:32: 33000000 INFO @ Tue, 16 Jun 2020 09:30:34: 26000000 INFO @ Tue, 16 Jun 2020 09:30:38: 23000000 INFO @ Tue, 16 Jun 2020 09:30:39: 34000000 INFO @ Tue, 16 Jun 2020 09:30:41: 27000000 INFO @ Tue, 16 Jun 2020 09:30:45: 24000000 INFO @ Tue, 16 Jun 2020 09:30:45: 35000000 INFO @ Tue, 16 Jun 2020 09:30:49: 28000000 INFO @ Tue, 16 Jun 2020 09:30:52: 25000000 INFO @ Tue, 16 Jun 2020 09:30:52: 36000000 INFO @ Tue, 16 Jun 2020 09:30:56: 29000000 INFO @ Tue, 16 Jun 2020 09:30:57: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:30:57: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:30:57: #1 total tags in treatment: 36623053 INFO @ Tue, 16 Jun 2020 09:30:57: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:30:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:30:57: #1 tags after filtering in treatment: 36623053 INFO @ Tue, 16 Jun 2020 09:30:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:30:57: #1 finished! INFO @ Tue, 16 Jun 2020 09:30:57: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:30:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:30:58: 26000000 INFO @ Tue, 16 Jun 2020 09:31:00: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:31:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:31:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494907/SRX494907.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494907/SRX494907.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494907/SRX494907.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494907/SRX494907.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:31:04: 30000000 INFO @ Tue, 16 Jun 2020 09:31:05: 27000000 INFO @ Tue, 16 Jun 2020 09:31:12: 31000000 INFO @ Tue, 16 Jun 2020 09:31:12: 28000000 INFO @ Tue, 16 Jun 2020 09:31:18: 29000000 INFO @ Tue, 16 Jun 2020 09:31:19: 32000000 INFO @ Tue, 16 Jun 2020 09:31:25: 30000000 INFO @ Tue, 16 Jun 2020 09:31:26: 33000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:31:32: 31000000 INFO @ Tue, 16 Jun 2020 09:31:33: 34000000 INFO @ Tue, 16 Jun 2020 09:31:38: 32000000 INFO @ Tue, 16 Jun 2020 09:31:41: 35000000 INFO @ Tue, 16 Jun 2020 09:31:44: 33000000 INFO @ Tue, 16 Jun 2020 09:31:49: 36000000 INFO @ Tue, 16 Jun 2020 09:31:51: 34000000 INFO @ Tue, 16 Jun 2020 09:31:53: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:31:53: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:31:53: #1 total tags in treatment: 36623053 INFO @ Tue, 16 Jun 2020 09:31:53: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:31:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:31:54: #1 tags after filtering in treatment: 36623053 INFO @ Tue, 16 Jun 2020 09:31:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:31:54: #1 finished! INFO @ Tue, 16 Jun 2020 09:31:54: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:31:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:31:56: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:31:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:31:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494907/SRX494907.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494907/SRX494907.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494907/SRX494907.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494907/SRX494907.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:31:57: 35000000 INFO @ Tue, 16 Jun 2020 09:32:03: 36000000 INFO @ Tue, 16 Jun 2020 09:32:07: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:32:07: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:32:07: #1 total tags in treatment: 36623053 INFO @ Tue, 16 Jun 2020 09:32:07: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:32:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:32:07: #1 tags after filtering in treatment: 36623053 INFO @ Tue, 16 Jun 2020 09:32:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:32:07: #1 finished! INFO @ Tue, 16 Jun 2020 09:32:07: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:32:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:32:10: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:32:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:32:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494907/SRX494907.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494907/SRX494907.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494907/SRX494907.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494907/SRX494907.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling