Job ID = 6368220 SRX = SRX494903 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:07:09 prefetch.2.10.7: 1) Downloading 'SRR1198435'... 2020-06-16T00:07:09 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:12:52 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:12:52 prefetch.2.10.7: 1) 'SRR1198435' was downloaded successfully Read 47950615 spots for SRR1198435/SRR1198435.sra Written 47950615 spots for SRR1198435/SRR1198435.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:00 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3138668 (6.55%) aligned 0 times 37486091 (78.18%) aligned exactly 1 time 7325856 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:11:00 Overall time: 00:11:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8189030 / 44811947 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:36:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494903/SRX494903.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494903/SRX494903.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494903/SRX494903.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494903/SRX494903.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:36:47: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:36:47: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:36:53: 1000000 INFO @ Tue, 16 Jun 2020 09:36:58: 2000000 INFO @ Tue, 16 Jun 2020 09:37:04: 3000000 INFO @ Tue, 16 Jun 2020 09:37:10: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:37:16: 5000000 INFO @ Tue, 16 Jun 2020 09:37:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494903/SRX494903.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494903/SRX494903.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494903/SRX494903.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494903/SRX494903.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:37:17: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:37:17: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:37:23: 6000000 INFO @ Tue, 16 Jun 2020 09:37:23: 1000000 INFO @ Tue, 16 Jun 2020 09:37:29: 7000000 INFO @ Tue, 16 Jun 2020 09:37:30: 2000000 INFO @ Tue, 16 Jun 2020 09:37:36: 8000000 INFO @ Tue, 16 Jun 2020 09:37:36: 3000000 INFO @ Tue, 16 Jun 2020 09:37:42: 9000000 INFO @ Tue, 16 Jun 2020 09:37:42: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:37:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494903/SRX494903.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494903/SRX494903.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494903/SRX494903.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494903/SRX494903.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:37:47: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:37:47: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:37:49: 10000000 INFO @ Tue, 16 Jun 2020 09:37:49: 5000000 INFO @ Tue, 16 Jun 2020 09:37:53: 1000000 INFO @ Tue, 16 Jun 2020 09:37:56: 11000000 INFO @ Tue, 16 Jun 2020 09:37:56: 6000000 INFO @ Tue, 16 Jun 2020 09:38:00: 2000000 INFO @ Tue, 16 Jun 2020 09:38:02: 12000000 INFO @ Tue, 16 Jun 2020 09:38:02: 7000000 INFO @ Tue, 16 Jun 2020 09:38:07: 3000000 INFO @ Tue, 16 Jun 2020 09:38:08: 13000000 INFO @ Tue, 16 Jun 2020 09:38:09: 8000000 INFO @ Tue, 16 Jun 2020 09:38:13: 4000000 INFO @ Tue, 16 Jun 2020 09:38:15: 14000000 INFO @ Tue, 16 Jun 2020 09:38:16: 9000000 INFO @ Tue, 16 Jun 2020 09:38:20: 5000000 INFO @ Tue, 16 Jun 2020 09:38:21: 15000000 INFO @ Tue, 16 Jun 2020 09:38:22: 10000000 INFO @ Tue, 16 Jun 2020 09:38:26: 6000000 INFO @ Tue, 16 Jun 2020 09:38:28: 16000000 INFO @ Tue, 16 Jun 2020 09:38:29: 11000000 INFO @ Tue, 16 Jun 2020 09:38:33: 7000000 INFO @ Tue, 16 Jun 2020 09:38:34: 17000000 INFO @ Tue, 16 Jun 2020 09:38:35: 12000000 INFO @ Tue, 16 Jun 2020 09:38:40: 8000000 INFO @ Tue, 16 Jun 2020 09:38:41: 18000000 INFO @ Tue, 16 Jun 2020 09:38:42: 13000000 INFO @ Tue, 16 Jun 2020 09:38:46: 9000000 INFO @ Tue, 16 Jun 2020 09:38:48: 19000000 INFO @ Tue, 16 Jun 2020 09:38:49: 14000000 INFO @ Tue, 16 Jun 2020 09:38:53: 10000000 INFO @ Tue, 16 Jun 2020 09:38:54: 20000000 INFO @ Tue, 16 Jun 2020 09:38:55: 15000000 INFO @ Tue, 16 Jun 2020 09:38:59: 11000000 INFO @ Tue, 16 Jun 2020 09:39:01: 21000000 INFO @ Tue, 16 Jun 2020 09:39:02: 16000000 INFO @ Tue, 16 Jun 2020 09:39:06: 12000000 INFO @ Tue, 16 Jun 2020 09:39:07: 22000000 INFO @ Tue, 16 Jun 2020 09:39:08: 17000000 INFO @ Tue, 16 Jun 2020 09:39:12: 13000000 INFO @ Tue, 16 Jun 2020 09:39:14: 23000000 INFO @ Tue, 16 Jun 2020 09:39:15: 18000000 INFO @ Tue, 16 Jun 2020 09:39:19: 14000000 INFO @ Tue, 16 Jun 2020 09:39:21: 24000000 INFO @ Tue, 16 Jun 2020 09:39:22: 19000000 INFO @ Tue, 16 Jun 2020 09:39:26: 15000000 INFO @ Tue, 16 Jun 2020 09:39:28: 25000000 INFO @ Tue, 16 Jun 2020 09:39:29: 20000000 INFO @ Tue, 16 Jun 2020 09:39:33: 16000000 INFO @ Tue, 16 Jun 2020 09:39:35: 26000000 INFO @ Tue, 16 Jun 2020 09:39:35: 21000000 INFO @ Tue, 16 Jun 2020 09:39:39: 17000000 INFO @ Tue, 16 Jun 2020 09:39:42: 27000000 INFO @ Tue, 16 Jun 2020 09:39:42: 22000000 INFO @ Tue, 16 Jun 2020 09:39:46: 18000000 INFO @ Tue, 16 Jun 2020 09:39:48: 28000000 INFO @ Tue, 16 Jun 2020 09:39:48: 23000000 INFO @ Tue, 16 Jun 2020 09:39:52: 19000000 INFO @ Tue, 16 Jun 2020 09:39:55: 29000000 INFO @ Tue, 16 Jun 2020 09:39:55: 24000000 INFO @ Tue, 16 Jun 2020 09:39:59: 20000000 INFO @ Tue, 16 Jun 2020 09:40:01: 30000000 INFO @ Tue, 16 Jun 2020 09:40:01: 25000000 INFO @ Tue, 16 Jun 2020 09:40:05: 21000000 INFO @ Tue, 16 Jun 2020 09:40:07: 31000000 INFO @ Tue, 16 Jun 2020 09:40:08: 26000000 INFO @ Tue, 16 Jun 2020 09:40:12: 22000000 INFO @ Tue, 16 Jun 2020 09:40:14: 32000000 INFO @ Tue, 16 Jun 2020 09:40:14: 27000000 INFO @ Tue, 16 Jun 2020 09:40:18: 23000000 INFO @ Tue, 16 Jun 2020 09:40:20: 33000000 INFO @ Tue, 16 Jun 2020 09:40:21: 28000000 INFO @ Tue, 16 Jun 2020 09:40:25: 24000000 INFO @ Tue, 16 Jun 2020 09:40:26: 34000000 INFO @ Tue, 16 Jun 2020 09:40:27: 29000000 INFO @ Tue, 16 Jun 2020 09:40:31: 25000000 INFO @ Tue, 16 Jun 2020 09:40:33: 35000000 INFO @ Tue, 16 Jun 2020 09:40:33: 30000000 INFO @ Tue, 16 Jun 2020 09:40:37: 26000000 INFO @ Tue, 16 Jun 2020 09:40:39: 36000000 INFO @ Tue, 16 Jun 2020 09:40:39: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:40:43: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:40:43: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:40:43: #1 total tags in treatment: 36622917 INFO @ Tue, 16 Jun 2020 09:40:43: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:40:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:40:43: #1 tags after filtering in treatment: 36622917 INFO @ Tue, 16 Jun 2020 09:40:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:40:43: #1 finished! INFO @ Tue, 16 Jun 2020 09:40:43: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:40:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:40:44: 27000000 INFO @ Tue, 16 Jun 2020 09:40:45: 32000000 INFO @ Tue, 16 Jun 2020 09:40:46: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:40:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:40:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494903/SRX494903.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494903/SRX494903.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494903/SRX494903.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494903/SRX494903.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:40:50: 28000000 INFO @ Tue, 16 Jun 2020 09:40:51: 33000000 INFO @ Tue, 16 Jun 2020 09:40:56: 29000000 INFO @ Tue, 16 Jun 2020 09:40:57: 34000000 INFO @ Tue, 16 Jun 2020 09:41:02: 30000000 INFO @ Tue, 16 Jun 2020 09:41:04: 35000000 INFO @ Tue, 16 Jun 2020 09:41:08: 31000000 INFO @ Tue, 16 Jun 2020 09:41:10: 36000000 INFO @ Tue, 16 Jun 2020 09:41:14: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:41:14: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:41:14: #1 total tags in treatment: 36622917 INFO @ Tue, 16 Jun 2020 09:41:14: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:41:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:41:14: 32000000 INFO @ Tue, 16 Jun 2020 09:41:14: #1 tags after filtering in treatment: 36622917 INFO @ Tue, 16 Jun 2020 09:41:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:41:14: #1 finished! INFO @ Tue, 16 Jun 2020 09:41:14: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:41:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:41:17: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:41:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:41:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494903/SRX494903.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494903/SRX494903.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494903/SRX494903.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494903/SRX494903.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:41:20: 33000000 INFO @ Tue, 16 Jun 2020 09:41:26: 34000000 INFO @ Tue, 16 Jun 2020 09:41:31: 35000000 INFO @ Tue, 16 Jun 2020 09:41:37: 36000000 INFO @ Tue, 16 Jun 2020 09:41:41: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:41:41: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:41:41: #1 total tags in treatment: 36622917 INFO @ Tue, 16 Jun 2020 09:41:41: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:41:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:41:41: #1 tags after filtering in treatment: 36622917 INFO @ Tue, 16 Jun 2020 09:41:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:41:41: #1 finished! INFO @ Tue, 16 Jun 2020 09:41:41: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:41:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:41:44: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:41:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:41:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494903/SRX494903.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494903/SRX494903.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494903/SRX494903.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494903/SRX494903.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。