Job ID = 6368212 SRX = SRX494895 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:09:54 prefetch.2.10.7: 1) Downloading 'SRR1198427'... 2020-06-16T00:09:54 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:16:46 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:16:46 prefetch.2.10.7: 1) 'SRR1198427' was downloaded successfully Read 47950615 spots for SRR1198427/SRR1198427.sra Written 47950615 spots for SRR1198427/SRR1198427.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:48 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3138726 (6.55%) aligned 0 times 37485785 (78.18%) aligned exactly 1 time 7326104 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:10:48 Overall time: 00:10:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8188982 / 44811889 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:40:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494895/SRX494895.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494895/SRX494895.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494895/SRX494895.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494895/SRX494895.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:40:41: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:40:41: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:40:47: 1000000 INFO @ Tue, 16 Jun 2020 09:40:52: 2000000 INFO @ Tue, 16 Jun 2020 09:40:58: 3000000 INFO @ Tue, 16 Jun 2020 09:41:04: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:41:10: 5000000 INFO @ Tue, 16 Jun 2020 09:41:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494895/SRX494895.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494895/SRX494895.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494895/SRX494895.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494895/SRX494895.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:41:11: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:41:11: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:41:16: 6000000 INFO @ Tue, 16 Jun 2020 09:41:18: 1000000 INFO @ Tue, 16 Jun 2020 09:41:22: 7000000 INFO @ Tue, 16 Jun 2020 09:41:24: 2000000 INFO @ Tue, 16 Jun 2020 09:41:28: 8000000 INFO @ Tue, 16 Jun 2020 09:41:30: 3000000 INFO @ Tue, 16 Jun 2020 09:41:34: 9000000 INFO @ Tue, 16 Jun 2020 09:41:36: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:41:40: 10000000 INFO @ Tue, 16 Jun 2020 09:41:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494895/SRX494895.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494895/SRX494895.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494895/SRX494895.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494895/SRX494895.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:41:41: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:41:41: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:41:43: 5000000 INFO @ Tue, 16 Jun 2020 09:41:47: 11000000 INFO @ Tue, 16 Jun 2020 09:41:47: 1000000 INFO @ Tue, 16 Jun 2020 09:41:49: 6000000 INFO @ Tue, 16 Jun 2020 09:41:53: 12000000 INFO @ Tue, 16 Jun 2020 09:41:53: 2000000 INFO @ Tue, 16 Jun 2020 09:41:55: 7000000 INFO @ Tue, 16 Jun 2020 09:41:59: 13000000 INFO @ Tue, 16 Jun 2020 09:42:00: 3000000 INFO @ Tue, 16 Jun 2020 09:42:02: 8000000 INFO @ Tue, 16 Jun 2020 09:42:06: 14000000 INFO @ Tue, 16 Jun 2020 09:42:06: 4000000 INFO @ Tue, 16 Jun 2020 09:42:09: 9000000 INFO @ Tue, 16 Jun 2020 09:42:12: 15000000 INFO @ Tue, 16 Jun 2020 09:42:13: 5000000 INFO @ Tue, 16 Jun 2020 09:42:15: 10000000 INFO @ Tue, 16 Jun 2020 09:42:19: 16000000 INFO @ Tue, 16 Jun 2020 09:42:19: 6000000 INFO @ Tue, 16 Jun 2020 09:42:21: 11000000 INFO @ Tue, 16 Jun 2020 09:42:25: 17000000 INFO @ Tue, 16 Jun 2020 09:42:25: 7000000 INFO @ Tue, 16 Jun 2020 09:42:28: 12000000 INFO @ Tue, 16 Jun 2020 09:42:31: 18000000 INFO @ Tue, 16 Jun 2020 09:42:32: 8000000 INFO @ Tue, 16 Jun 2020 09:42:35: 13000000 INFO @ Tue, 16 Jun 2020 09:42:38: 9000000 INFO @ Tue, 16 Jun 2020 09:42:38: 19000000 INFO @ Tue, 16 Jun 2020 09:42:41: 14000000 INFO @ Tue, 16 Jun 2020 09:42:45: 10000000 INFO @ Tue, 16 Jun 2020 09:42:45: 20000000 INFO @ Tue, 16 Jun 2020 09:42:48: 15000000 INFO @ Tue, 16 Jun 2020 09:42:51: 11000000 INFO @ Tue, 16 Jun 2020 09:42:51: 21000000 INFO @ Tue, 16 Jun 2020 09:42:54: 16000000 INFO @ Tue, 16 Jun 2020 09:42:57: 12000000 INFO @ Tue, 16 Jun 2020 09:42:58: 22000000 INFO @ Tue, 16 Jun 2020 09:43:01: 17000000 INFO @ Tue, 16 Jun 2020 09:43:04: 13000000 INFO @ Tue, 16 Jun 2020 09:43:04: 23000000 INFO @ Tue, 16 Jun 2020 09:43:07: 18000000 INFO @ Tue, 16 Jun 2020 09:43:10: 14000000 INFO @ Tue, 16 Jun 2020 09:43:10: 24000000 INFO @ Tue, 16 Jun 2020 09:43:14: 19000000 INFO @ Tue, 16 Jun 2020 09:43:17: 15000000 INFO @ Tue, 16 Jun 2020 09:43:17: 25000000 INFO @ Tue, 16 Jun 2020 09:43:21: 20000000 INFO @ Tue, 16 Jun 2020 09:43:23: 26000000 INFO @ Tue, 16 Jun 2020 09:43:24: 16000000 INFO @ Tue, 16 Jun 2020 09:43:28: 21000000 INFO @ Tue, 16 Jun 2020 09:43:30: 27000000 INFO @ Tue, 16 Jun 2020 09:43:30: 17000000 INFO @ Tue, 16 Jun 2020 09:43:35: 22000000 INFO @ Tue, 16 Jun 2020 09:43:37: 28000000 INFO @ Tue, 16 Jun 2020 09:43:37: 18000000 INFO @ Tue, 16 Jun 2020 09:43:41: 23000000 INFO @ Tue, 16 Jun 2020 09:43:43: 29000000 INFO @ Tue, 16 Jun 2020 09:43:43: 19000000 INFO @ Tue, 16 Jun 2020 09:43:47: 24000000 INFO @ Tue, 16 Jun 2020 09:43:49: 30000000 INFO @ Tue, 16 Jun 2020 09:43:50: 20000000 INFO @ Tue, 16 Jun 2020 09:43:54: 25000000 INFO @ Tue, 16 Jun 2020 09:43:56: 31000000 INFO @ Tue, 16 Jun 2020 09:43:57: 21000000 INFO @ Tue, 16 Jun 2020 09:44:00: 26000000 INFO @ Tue, 16 Jun 2020 09:44:03: 32000000 INFO @ Tue, 16 Jun 2020 09:44:03: 22000000 INFO @ Tue, 16 Jun 2020 09:44:07: 27000000 INFO @ Tue, 16 Jun 2020 09:44:09: 33000000 INFO @ Tue, 16 Jun 2020 09:44:10: 23000000 INFO @ Tue, 16 Jun 2020 09:44:13: 28000000 INFO @ Tue, 16 Jun 2020 09:44:15: 34000000 INFO @ Tue, 16 Jun 2020 09:44:16: 24000000 INFO @ Tue, 16 Jun 2020 09:44:19: 29000000 INFO @ Tue, 16 Jun 2020 09:44:22: 35000000 INFO @ Tue, 16 Jun 2020 09:44:23: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:44:26: 30000000 INFO @ Tue, 16 Jun 2020 09:44:28: 36000000 INFO @ Tue, 16 Jun 2020 09:44:29: 26000000 INFO @ Tue, 16 Jun 2020 09:44:32: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:44:32: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:44:32: #1 total tags in treatment: 36622907 INFO @ Tue, 16 Jun 2020 09:44:32: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:44:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:44:33: 31000000 INFO @ Tue, 16 Jun 2020 09:44:33: #1 tags after filtering in treatment: 36622907 INFO @ Tue, 16 Jun 2020 09:44:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:44:33: #1 finished! INFO @ Tue, 16 Jun 2020 09:44:33: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:44:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:44:35: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:44:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:44:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494895/SRX494895.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494895/SRX494895.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494895/SRX494895.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494895/SRX494895.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:44:36: 27000000 INFO @ Tue, 16 Jun 2020 09:44:39: 32000000 INFO @ Tue, 16 Jun 2020 09:44:42: 28000000 INFO @ Tue, 16 Jun 2020 09:44:45: 33000000 INFO @ Tue, 16 Jun 2020 09:44:48: 29000000 INFO @ Tue, 16 Jun 2020 09:44:51: 34000000 INFO @ Tue, 16 Jun 2020 09:44:54: 30000000 INFO @ Tue, 16 Jun 2020 09:44:57: 35000000 INFO @ Tue, 16 Jun 2020 09:45:00: 31000000 INFO @ Tue, 16 Jun 2020 09:45:03: 36000000 INFO @ Tue, 16 Jun 2020 09:45:06: 32000000 INFO @ Tue, 16 Jun 2020 09:45:07: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:45:07: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:45:07: #1 total tags in treatment: 36622907 INFO @ Tue, 16 Jun 2020 09:45:07: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:45:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:45:08: #1 tags after filtering in treatment: 36622907 INFO @ Tue, 16 Jun 2020 09:45:08: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:45:08: #1 finished! INFO @ Tue, 16 Jun 2020 09:45:08: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:45:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:45:10: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:45:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:45:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494895/SRX494895.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494895/SRX494895.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494895/SRX494895.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494895/SRX494895.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:45:12: 33000000 INFO @ Tue, 16 Jun 2020 09:45:18: 34000000 INFO @ Tue, 16 Jun 2020 09:45:23: 35000000 INFO @ Tue, 16 Jun 2020 09:45:29: 36000000 INFO @ Tue, 16 Jun 2020 09:45:33: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:45:33: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:45:33: #1 total tags in treatment: 36622907 INFO @ Tue, 16 Jun 2020 09:45:33: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:45:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:45:34: #1 tags after filtering in treatment: 36622907 INFO @ Tue, 16 Jun 2020 09:45:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:45:34: #1 finished! INFO @ Tue, 16 Jun 2020 09:45:34: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:45:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:45:36: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:45:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:45:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494895/SRX494895.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494895/SRX494895.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494895/SRX494895.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494895/SRX494895.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling