Job ID = 6368180 SRX = SRX494864 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:11:20 prefetch.2.10.7: 1) Downloading 'SRR1198396'... 2020-06-16T00:11:20 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:18:49 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:18:49 prefetch.2.10.7: 1) 'SRR1198396' was downloaded successfully Read 69236731 spots for SRR1198396/SRR1198396.sra Written 69236731 spots for SRR1198396/SRR1198396.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:40 69236731 reads; of these: 69236731 (100.00%) were unpaired; of these: 3484204 (5.03%) aligned 0 times 54816854 (79.17%) aligned exactly 1 time 10935673 (15.79%) aligned >1 times 94.97% overall alignment rate Time searching: 00:15:40 Overall time: 00:15:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 28147743 / 65752527 = 0.4281 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:50:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494864/SRX494864.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494864/SRX494864.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494864/SRX494864.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494864/SRX494864.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:50:54: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:50:54: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:50:59: 1000000 INFO @ Tue, 16 Jun 2020 09:51:04: 2000000 INFO @ Tue, 16 Jun 2020 09:51:10: 3000000 INFO @ Tue, 16 Jun 2020 09:51:15: 4000000 INFO @ Tue, 16 Jun 2020 09:51:20: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:51:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494864/SRX494864.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494864/SRX494864.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494864/SRX494864.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494864/SRX494864.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:51:24: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:51:24: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:51:25: 6000000 INFO @ Tue, 16 Jun 2020 09:51:30: 1000000 INFO @ Tue, 16 Jun 2020 09:51:31: 7000000 INFO @ Tue, 16 Jun 2020 09:51:35: 2000000 INFO @ Tue, 16 Jun 2020 09:51:36: 8000000 INFO @ Tue, 16 Jun 2020 09:51:40: 3000000 INFO @ Tue, 16 Jun 2020 09:51:41: 9000000 INFO @ Tue, 16 Jun 2020 09:51:46: 4000000 INFO @ Tue, 16 Jun 2020 09:51:47: 10000000 INFO @ Tue, 16 Jun 2020 09:51:51: 5000000 INFO @ Tue, 16 Jun 2020 09:51:52: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:51:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494864/SRX494864.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494864/SRX494864.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494864/SRX494864.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494864/SRX494864.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:51:54: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:51:54: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:51:57: 6000000 INFO @ Tue, 16 Jun 2020 09:51:57: 12000000 INFO @ Tue, 16 Jun 2020 09:52:00: 1000000 INFO @ Tue, 16 Jun 2020 09:52:02: 7000000 INFO @ Tue, 16 Jun 2020 09:52:03: 13000000 INFO @ Tue, 16 Jun 2020 09:52:05: 2000000 INFO @ Tue, 16 Jun 2020 09:52:07: 8000000 INFO @ Tue, 16 Jun 2020 09:52:08: 14000000 INFO @ Tue, 16 Jun 2020 09:52:10: 3000000 INFO @ Tue, 16 Jun 2020 09:52:13: 9000000 INFO @ Tue, 16 Jun 2020 09:52:13: 15000000 INFO @ Tue, 16 Jun 2020 09:52:16: 4000000 INFO @ Tue, 16 Jun 2020 09:52:18: 10000000 INFO @ Tue, 16 Jun 2020 09:52:19: 16000000 INFO @ Tue, 16 Jun 2020 09:52:21: 5000000 INFO @ Tue, 16 Jun 2020 09:52:24: 11000000 INFO @ Tue, 16 Jun 2020 09:52:24: 17000000 INFO @ Tue, 16 Jun 2020 09:52:27: 6000000 INFO @ Tue, 16 Jun 2020 09:52:29: 12000000 INFO @ Tue, 16 Jun 2020 09:52:29: 18000000 INFO @ Tue, 16 Jun 2020 09:52:32: 7000000 INFO @ Tue, 16 Jun 2020 09:52:34: 13000000 INFO @ Tue, 16 Jun 2020 09:52:35: 19000000 INFO @ Tue, 16 Jun 2020 09:52:38: 8000000 INFO @ Tue, 16 Jun 2020 09:52:40: 14000000 INFO @ Tue, 16 Jun 2020 09:52:40: 20000000 INFO @ Tue, 16 Jun 2020 09:52:43: 9000000 INFO @ Tue, 16 Jun 2020 09:52:45: 15000000 INFO @ Tue, 16 Jun 2020 09:52:45: 21000000 INFO @ Tue, 16 Jun 2020 09:52:49: 10000000 INFO @ Tue, 16 Jun 2020 09:52:51: 16000000 INFO @ Tue, 16 Jun 2020 09:52:51: 22000000 INFO @ Tue, 16 Jun 2020 09:52:54: 11000000 INFO @ Tue, 16 Jun 2020 09:52:56: 17000000 INFO @ Tue, 16 Jun 2020 09:52:56: 23000000 INFO @ Tue, 16 Jun 2020 09:52:59: 12000000 INFO @ Tue, 16 Jun 2020 09:53:01: 18000000 INFO @ Tue, 16 Jun 2020 09:53:01: 24000000 INFO @ Tue, 16 Jun 2020 09:53:05: 13000000 INFO @ Tue, 16 Jun 2020 09:53:07: 19000000 INFO @ Tue, 16 Jun 2020 09:53:07: 25000000 INFO @ Tue, 16 Jun 2020 09:53:10: 14000000 INFO @ Tue, 16 Jun 2020 09:53:12: 20000000 INFO @ Tue, 16 Jun 2020 09:53:12: 26000000 INFO @ Tue, 16 Jun 2020 09:53:15: 15000000 INFO @ Tue, 16 Jun 2020 09:53:17: 21000000 INFO @ Tue, 16 Jun 2020 09:53:17: 27000000 INFO @ Tue, 16 Jun 2020 09:53:21: 16000000 INFO @ Tue, 16 Jun 2020 09:53:22: 22000000 INFO @ Tue, 16 Jun 2020 09:53:22: 28000000 INFO @ Tue, 16 Jun 2020 09:53:26: 17000000 INFO @ Tue, 16 Jun 2020 09:53:27: 23000000 INFO @ Tue, 16 Jun 2020 09:53:28: 29000000 INFO @ Tue, 16 Jun 2020 09:53:31: 18000000 INFO @ Tue, 16 Jun 2020 09:53:33: 24000000 INFO @ Tue, 16 Jun 2020 09:53:33: 30000000 INFO @ Tue, 16 Jun 2020 09:53:36: 19000000 INFO @ Tue, 16 Jun 2020 09:53:38: 25000000 INFO @ Tue, 16 Jun 2020 09:53:38: 31000000 INFO @ Tue, 16 Jun 2020 09:53:42: 20000000 INFO @ Tue, 16 Jun 2020 09:53:43: 32000000 INFO @ Tue, 16 Jun 2020 09:53:43: 26000000 INFO @ Tue, 16 Jun 2020 09:53:47: 21000000 INFO @ Tue, 16 Jun 2020 09:53:48: 33000000 INFO @ Tue, 16 Jun 2020 09:53:48: 27000000 INFO @ Tue, 16 Jun 2020 09:53:52: 22000000 INFO @ Tue, 16 Jun 2020 09:53:53: 34000000 INFO @ Tue, 16 Jun 2020 09:53:54: 28000000 INFO @ Tue, 16 Jun 2020 09:53:57: 23000000 INFO @ Tue, 16 Jun 2020 09:53:59: 35000000 INFO @ Tue, 16 Jun 2020 09:53:59: 29000000 INFO @ Tue, 16 Jun 2020 09:54:02: 24000000 INFO @ Tue, 16 Jun 2020 09:54:04: 36000000 INFO @ Tue, 16 Jun 2020 09:54:04: 30000000 INFO @ Tue, 16 Jun 2020 09:54:07: 25000000 INFO @ Tue, 16 Jun 2020 09:54:09: 37000000 INFO @ Tue, 16 Jun 2020 09:54:09: 31000000 INFO @ Tue, 16 Jun 2020 09:54:13: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:54:13: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:54:13: #1 total tags in treatment: 37604784 INFO @ Tue, 16 Jun 2020 09:54:13: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:54:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:54:13: 26000000 INFO @ Tue, 16 Jun 2020 09:54:13: #1 tags after filtering in treatment: 37604784 INFO @ Tue, 16 Jun 2020 09:54:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:54:13: #1 finished! INFO @ Tue, 16 Jun 2020 09:54:13: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:54:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:54:14: 32000000 INFO @ Tue, 16 Jun 2020 09:54:16: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:54:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:54:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494864/SRX494864.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494864/SRX494864.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494864/SRX494864.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494864/SRX494864.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:54:18: 27000000 INFO @ Tue, 16 Jun 2020 09:54:20: 33000000 INFO @ Tue, 16 Jun 2020 09:54:23: 28000000 INFO @ Tue, 16 Jun 2020 09:54:25: 34000000 INFO @ Tue, 16 Jun 2020 09:54:28: 29000000 INFO @ Tue, 16 Jun 2020 09:54:30: 35000000 INFO @ Tue, 16 Jun 2020 09:54:33: 30000000 INFO @ Tue, 16 Jun 2020 09:54:35: 36000000 INFO @ Tue, 16 Jun 2020 09:54:39: 31000000 INFO @ Tue, 16 Jun 2020 09:54:40: 37000000 INFO @ Tue, 16 Jun 2020 09:54:44: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:54:44: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:54:44: #1 total tags in treatment: 37604784 INFO @ Tue, 16 Jun 2020 09:54:44: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:54:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:54:44: 32000000 INFO @ Tue, 16 Jun 2020 09:54:44: #1 tags after filtering in treatment: 37604784 INFO @ Tue, 16 Jun 2020 09:54:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:54:44: #1 finished! INFO @ Tue, 16 Jun 2020 09:54:44: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:54:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:54:47: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:54:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:54:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494864/SRX494864.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494864/SRX494864.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494864/SRX494864.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494864/SRX494864.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:54:49: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:54:54: 34000000 INFO @ Tue, 16 Jun 2020 09:54:59: 35000000 INFO @ Tue, 16 Jun 2020 09:55:04: 36000000 INFO @ Tue, 16 Jun 2020 09:55:08: 37000000 INFO @ Tue, 16 Jun 2020 09:55:12: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:55:12: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:55:12: #1 total tags in treatment: 37604784 INFO @ Tue, 16 Jun 2020 09:55:12: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:55:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:55:12: #1 tags after filtering in treatment: 37604784 INFO @ Tue, 16 Jun 2020 09:55:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:55:12: #1 finished! INFO @ Tue, 16 Jun 2020 09:55:12: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:55:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:55:15: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:55:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:55:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494864/SRX494864.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494864/SRX494864.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494864/SRX494864.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494864/SRX494864.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。