Job ID = 6368106 SRX = SRX466569 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:09:39 prefetch.2.10.7: 1) Downloading 'SRR1163635'... 2020-06-16T00:09:39 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:15:26 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:15:26 prefetch.2.10.7: 1) 'SRR1163635' was downloaded successfully Read 51067685 spots for SRR1163635/SRR1163635.sra Written 51067685 spots for SRR1163635/SRR1163635.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:31 51067685 reads; of these: 51067685 (100.00%) were unpaired; of these: 4585332 (8.98%) aligned 0 times 37934884 (74.28%) aligned exactly 1 time 8547469 (16.74%) aligned >1 times 91.02% overall alignment rate Time searching: 00:11:31 Overall time: 00:11:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 9745675 / 46482353 = 0.2097 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:40:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX466569/SRX466569.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX466569/SRX466569.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX466569/SRX466569.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX466569/SRX466569.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:40:22: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:40:22: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:40:30: 1000000 INFO @ Tue, 16 Jun 2020 09:40:37: 2000000 INFO @ Tue, 16 Jun 2020 09:40:44: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:40:52: 4000000 INFO @ Tue, 16 Jun 2020 09:40:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX466569/SRX466569.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX466569/SRX466569.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX466569/SRX466569.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX466569/SRX466569.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:40:52: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:40:52: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:41:00: 5000000 INFO @ Tue, 16 Jun 2020 09:41:00: 1000000 INFO @ Tue, 16 Jun 2020 09:41:08: 6000000 INFO @ Tue, 16 Jun 2020 09:41:09: 2000000 INFO @ Tue, 16 Jun 2020 09:41:16: 7000000 INFO @ Tue, 16 Jun 2020 09:41:17: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:41:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX466569/SRX466569.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX466569/SRX466569.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX466569/SRX466569.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX466569/SRX466569.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:41:22: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:41:22: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:41:24: 8000000 INFO @ Tue, 16 Jun 2020 09:41:25: 4000000 INFO @ Tue, 16 Jun 2020 09:41:31: 1000000 INFO @ Tue, 16 Jun 2020 09:41:33: 9000000 INFO @ Tue, 16 Jun 2020 09:41:33: 5000000 INFO @ Tue, 16 Jun 2020 09:41:39: 2000000 INFO @ Tue, 16 Jun 2020 09:41:41: 10000000 INFO @ Tue, 16 Jun 2020 09:41:42: 6000000 INFO @ Tue, 16 Jun 2020 09:41:47: 3000000 INFO @ Tue, 16 Jun 2020 09:41:49: 11000000 INFO @ Tue, 16 Jun 2020 09:41:50: 7000000 INFO @ Tue, 16 Jun 2020 09:41:56: 4000000 INFO @ Tue, 16 Jun 2020 09:41:58: 12000000 INFO @ Tue, 16 Jun 2020 09:41:59: 8000000 INFO @ Tue, 16 Jun 2020 09:42:04: 5000000 INFO @ Tue, 16 Jun 2020 09:42:06: 13000000 INFO @ Tue, 16 Jun 2020 09:42:07: 9000000 INFO @ Tue, 16 Jun 2020 09:42:13: 6000000 INFO @ Tue, 16 Jun 2020 09:42:15: 14000000 INFO @ Tue, 16 Jun 2020 09:42:15: 10000000 INFO @ Tue, 16 Jun 2020 09:42:21: 7000000 INFO @ Tue, 16 Jun 2020 09:42:23: 15000000 INFO @ Tue, 16 Jun 2020 09:42:23: 11000000 INFO @ Tue, 16 Jun 2020 09:42:29: 8000000 INFO @ Tue, 16 Jun 2020 09:42:31: 16000000 INFO @ Tue, 16 Jun 2020 09:42:32: 12000000 INFO @ Tue, 16 Jun 2020 09:42:37: 9000000 INFO @ Tue, 16 Jun 2020 09:42:39: 17000000 INFO @ Tue, 16 Jun 2020 09:42:40: 13000000 INFO @ Tue, 16 Jun 2020 09:42:45: 10000000 INFO @ Tue, 16 Jun 2020 09:42:47: 18000000 INFO @ Tue, 16 Jun 2020 09:42:48: 14000000 INFO @ Tue, 16 Jun 2020 09:42:53: 11000000 INFO @ Tue, 16 Jun 2020 09:42:55: 19000000 INFO @ Tue, 16 Jun 2020 09:42:56: 15000000 INFO @ Tue, 16 Jun 2020 09:43:01: 12000000 INFO @ Tue, 16 Jun 2020 09:43:03: 20000000 INFO @ Tue, 16 Jun 2020 09:43:04: 16000000 INFO @ Tue, 16 Jun 2020 09:43:09: 13000000 INFO @ Tue, 16 Jun 2020 09:43:11: 21000000 INFO @ Tue, 16 Jun 2020 09:43:12: 17000000 INFO @ Tue, 16 Jun 2020 09:43:17: 14000000 INFO @ Tue, 16 Jun 2020 09:43:19: 22000000 INFO @ Tue, 16 Jun 2020 09:43:20: 18000000 INFO @ Tue, 16 Jun 2020 09:43:25: 15000000 INFO @ Tue, 16 Jun 2020 09:43:27: 23000000 INFO @ Tue, 16 Jun 2020 09:43:28: 19000000 INFO @ Tue, 16 Jun 2020 09:43:33: 16000000 INFO @ Tue, 16 Jun 2020 09:43:35: 24000000 INFO @ Tue, 16 Jun 2020 09:43:36: 20000000 INFO @ Tue, 16 Jun 2020 09:43:41: 17000000 INFO @ Tue, 16 Jun 2020 09:43:43: 25000000 INFO @ Tue, 16 Jun 2020 09:43:44: 21000000 INFO @ Tue, 16 Jun 2020 09:43:49: 18000000 INFO @ Tue, 16 Jun 2020 09:43:51: 22000000 INFO @ Tue, 16 Jun 2020 09:43:51: 26000000 INFO @ Tue, 16 Jun 2020 09:43:57: 19000000 INFO @ Tue, 16 Jun 2020 09:43:59: 23000000 INFO @ Tue, 16 Jun 2020 09:43:59: 27000000 INFO @ Tue, 16 Jun 2020 09:44:05: 20000000 INFO @ Tue, 16 Jun 2020 09:44:07: 24000000 INFO @ Tue, 16 Jun 2020 09:44:07: 28000000 INFO @ Tue, 16 Jun 2020 09:44:13: 21000000 INFO @ Tue, 16 Jun 2020 09:44:15: 25000000 INFO @ Tue, 16 Jun 2020 09:44:15: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:44:21: 22000000 INFO @ Tue, 16 Jun 2020 09:44:23: 26000000 INFO @ Tue, 16 Jun 2020 09:44:23: 30000000 INFO @ Tue, 16 Jun 2020 09:44:29: 23000000 INFO @ Tue, 16 Jun 2020 09:44:31: 27000000 INFO @ Tue, 16 Jun 2020 09:44:31: 31000000 INFO @ Tue, 16 Jun 2020 09:44:37: 24000000 INFO @ Tue, 16 Jun 2020 09:44:38: 28000000 INFO @ Tue, 16 Jun 2020 09:44:39: 32000000 INFO @ Tue, 16 Jun 2020 09:44:45: 25000000 INFO @ Tue, 16 Jun 2020 09:44:46: 29000000 INFO @ Tue, 16 Jun 2020 09:44:47: 33000000 INFO @ Tue, 16 Jun 2020 09:44:53: 26000000 INFO @ Tue, 16 Jun 2020 09:44:54: 30000000 INFO @ Tue, 16 Jun 2020 09:44:54: 34000000 INFO @ Tue, 16 Jun 2020 09:45:00: 27000000 INFO @ Tue, 16 Jun 2020 09:45:02: 31000000 INFO @ Tue, 16 Jun 2020 09:45:03: 35000000 INFO @ Tue, 16 Jun 2020 09:45:09: 28000000 INFO @ Tue, 16 Jun 2020 09:45:10: 32000000 INFO @ Tue, 16 Jun 2020 09:45:11: 36000000 INFO @ Tue, 16 Jun 2020 09:45:17: 29000000 INFO @ Tue, 16 Jun 2020 09:45:17: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:45:17: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:45:17: #1 total tags in treatment: 36736678 INFO @ Tue, 16 Jun 2020 09:45:17: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:45:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:45:17: #1 tags after filtering in treatment: 36736678 INFO @ Tue, 16 Jun 2020 09:45:17: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:45:17: #1 finished! INFO @ Tue, 16 Jun 2020 09:45:17: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:45:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:45:18: 33000000 INFO @ Tue, 16 Jun 2020 09:45:19: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:45:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:45:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX466569/SRX466569.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX466569/SRX466569.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX466569/SRX466569.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX466569/SRX466569.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:45:24: 30000000 INFO @ Tue, 16 Jun 2020 09:45:27: 34000000 INFO @ Tue, 16 Jun 2020 09:45:32: 31000000 INFO @ Tue, 16 Jun 2020 09:45:35: 35000000 INFO @ Tue, 16 Jun 2020 09:45:40: 32000000 INFO @ Tue, 16 Jun 2020 09:45:43: 36000000 INFO @ Tue, 16 Jun 2020 09:45:48: 33000000 INFO @ Tue, 16 Jun 2020 09:45:49: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:45:49: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:45:49: #1 total tags in treatment: 36736678 INFO @ Tue, 16 Jun 2020 09:45:49: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:45:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:45:49: #1 tags after filtering in treatment: 36736678 INFO @ Tue, 16 Jun 2020 09:45:49: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:45:49: #1 finished! INFO @ Tue, 16 Jun 2020 09:45:49: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:45:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:45:52: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:45:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:45:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX466569/SRX466569.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX466569/SRX466569.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX466569/SRX466569.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX466569/SRX466569.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:45:55: 34000000 INFO @ Tue, 16 Jun 2020 09:46:03: 35000000 INFO @ Tue, 16 Jun 2020 09:46:10: 36000000 INFO @ Tue, 16 Jun 2020 09:46:15: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:46:15: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:46:15: #1 total tags in treatment: 36736678 INFO @ Tue, 16 Jun 2020 09:46:15: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:46:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:46:16: #1 tags after filtering in treatment: 36736678 INFO @ Tue, 16 Jun 2020 09:46:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:46:16: #1 finished! INFO @ Tue, 16 Jun 2020 09:46:16: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:46:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:46:18: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:46:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:46:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX466569/SRX466569.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX466569/SRX466569.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX466569/SRX466569.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX466569/SRX466569.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling