Job ID = 6368101 SRX = SRX466564 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:52:03 prefetch.2.10.7: 1) Downloading 'SRR1163630'... 2020-06-15T23:52:03 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:57:40 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:57:40 prefetch.2.10.7: 1) 'SRR1163630' was downloaded successfully Read 47950615 spots for SRR1163630/SRR1163630.sra Written 47950615 spots for SRR1163630/SRR1163630.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:51 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3138698 (6.55%) aligned 0 times 37485983 (78.18%) aligned exactly 1 time 7325934 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:10:51 Overall time: 00:10:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8189288 / 44811917 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:21:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX466564/SRX466564.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX466564/SRX466564.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX466564/SRX466564.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX466564/SRX466564.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:21:56: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:21:56: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:22:04: 1000000 INFO @ Tue, 16 Jun 2020 09:22:11: 2000000 INFO @ Tue, 16 Jun 2020 09:22:18: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:22:25: 4000000 INFO @ Tue, 16 Jun 2020 09:22:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX466564/SRX466564.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX466564/SRX466564.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX466564/SRX466564.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX466564/SRX466564.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:22:26: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:22:26: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:22:33: 5000000 INFO @ Tue, 16 Jun 2020 09:22:34: 1000000 INFO @ Tue, 16 Jun 2020 09:22:41: 6000000 INFO @ Tue, 16 Jun 2020 09:22:42: 2000000 INFO @ Tue, 16 Jun 2020 09:22:48: 7000000 INFO @ Tue, 16 Jun 2020 09:22:49: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:22:56: 8000000 INFO @ Tue, 16 Jun 2020 09:22:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX466564/SRX466564.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX466564/SRX466564.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX466564/SRX466564.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX466564/SRX466564.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:22:56: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:22:56: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:22:57: 4000000 INFO @ Tue, 16 Jun 2020 09:23:03: 9000000 INFO @ Tue, 16 Jun 2020 09:23:04: 1000000 INFO @ Tue, 16 Jun 2020 09:23:05: 5000000 INFO @ Tue, 16 Jun 2020 09:23:11: 10000000 INFO @ Tue, 16 Jun 2020 09:23:12: 2000000 INFO @ Tue, 16 Jun 2020 09:23:12: 6000000 INFO @ Tue, 16 Jun 2020 09:23:18: 11000000 INFO @ Tue, 16 Jun 2020 09:23:19: 3000000 INFO @ Tue, 16 Jun 2020 09:23:20: 7000000 INFO @ Tue, 16 Jun 2020 09:23:26: 12000000 INFO @ Tue, 16 Jun 2020 09:23:27: 4000000 INFO @ Tue, 16 Jun 2020 09:23:28: 8000000 INFO @ Tue, 16 Jun 2020 09:23:33: 13000000 INFO @ Tue, 16 Jun 2020 09:23:35: 5000000 INFO @ Tue, 16 Jun 2020 09:23:36: 9000000 INFO @ Tue, 16 Jun 2020 09:23:41: 14000000 INFO @ Tue, 16 Jun 2020 09:23:42: 6000000 INFO @ Tue, 16 Jun 2020 09:23:43: 10000000 INFO @ Tue, 16 Jun 2020 09:23:48: 15000000 INFO @ Tue, 16 Jun 2020 09:23:50: 7000000 INFO @ Tue, 16 Jun 2020 09:23:51: 11000000 INFO @ Tue, 16 Jun 2020 09:23:56: 16000000 INFO @ Tue, 16 Jun 2020 09:23:57: 8000000 INFO @ Tue, 16 Jun 2020 09:23:58: 12000000 INFO @ Tue, 16 Jun 2020 09:24:03: 17000000 INFO @ Tue, 16 Jun 2020 09:24:05: 9000000 INFO @ Tue, 16 Jun 2020 09:24:06: 13000000 INFO @ Tue, 16 Jun 2020 09:24:11: 18000000 INFO @ Tue, 16 Jun 2020 09:24:12: 10000000 INFO @ Tue, 16 Jun 2020 09:24:14: 14000000 INFO @ Tue, 16 Jun 2020 09:24:19: 19000000 INFO @ Tue, 16 Jun 2020 09:24:20: 11000000 INFO @ Tue, 16 Jun 2020 09:24:21: 15000000 INFO @ Tue, 16 Jun 2020 09:24:26: 20000000 INFO @ Tue, 16 Jun 2020 09:24:28: 12000000 INFO @ Tue, 16 Jun 2020 09:24:29: 16000000 INFO @ Tue, 16 Jun 2020 09:24:34: 21000000 INFO @ Tue, 16 Jun 2020 09:24:35: 13000000 INFO @ Tue, 16 Jun 2020 09:24:37: 17000000 INFO @ Tue, 16 Jun 2020 09:24:41: 22000000 INFO @ Tue, 16 Jun 2020 09:24:43: 14000000 INFO @ Tue, 16 Jun 2020 09:24:44: 18000000 INFO @ Tue, 16 Jun 2020 09:24:49: 23000000 INFO @ Tue, 16 Jun 2020 09:24:50: 15000000 INFO @ Tue, 16 Jun 2020 09:24:52: 19000000 INFO @ Tue, 16 Jun 2020 09:24:57: 24000000 INFO @ Tue, 16 Jun 2020 09:24:58: 16000000 INFO @ Tue, 16 Jun 2020 09:24:59: 20000000 INFO @ Tue, 16 Jun 2020 09:25:05: 25000000 INFO @ Tue, 16 Jun 2020 09:25:06: 17000000 INFO @ Tue, 16 Jun 2020 09:25:07: 21000000 INFO @ Tue, 16 Jun 2020 09:25:13: 26000000 INFO @ Tue, 16 Jun 2020 09:25:13: 18000000 INFO @ Tue, 16 Jun 2020 09:25:15: 22000000 INFO @ Tue, 16 Jun 2020 09:25:20: 27000000 INFO @ Tue, 16 Jun 2020 09:25:21: 19000000 INFO @ Tue, 16 Jun 2020 09:25:22: 23000000 INFO @ Tue, 16 Jun 2020 09:25:28: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:25:29: 20000000 INFO @ Tue, 16 Jun 2020 09:25:30: 24000000 INFO @ Tue, 16 Jun 2020 09:25:36: 21000000 INFO @ Tue, 16 Jun 2020 09:25:36: 29000000 INFO @ Tue, 16 Jun 2020 09:25:38: 25000000 INFO @ Tue, 16 Jun 2020 09:25:44: 22000000 INFO @ Tue, 16 Jun 2020 09:25:45: 30000000 INFO @ Tue, 16 Jun 2020 09:25:46: 26000000 INFO @ Tue, 16 Jun 2020 09:25:52: 23000000 INFO @ Tue, 16 Jun 2020 09:25:53: 31000000 INFO @ Tue, 16 Jun 2020 09:25:54: 27000000 INFO @ Tue, 16 Jun 2020 09:26:00: 24000000 INFO @ Tue, 16 Jun 2020 09:26:01: 32000000 INFO @ Tue, 16 Jun 2020 09:26:01: 28000000 INFO @ Tue, 16 Jun 2020 09:26:07: 25000000 INFO @ Tue, 16 Jun 2020 09:26:09: 29000000 INFO @ Tue, 16 Jun 2020 09:26:09: 33000000 INFO @ Tue, 16 Jun 2020 09:26:15: 26000000 INFO @ Tue, 16 Jun 2020 09:26:17: 30000000 INFO @ Tue, 16 Jun 2020 09:26:18: 34000000 INFO @ Tue, 16 Jun 2020 09:26:23: 27000000 INFO @ Tue, 16 Jun 2020 09:26:25: 31000000 INFO @ Tue, 16 Jun 2020 09:26:26: 35000000 INFO @ Tue, 16 Jun 2020 09:26:31: 28000000 INFO @ Tue, 16 Jun 2020 09:26:33: 32000000 INFO @ Tue, 16 Jun 2020 09:26:34: 36000000 INFO @ Tue, 16 Jun 2020 09:26:39: 29000000 INFO @ Tue, 16 Jun 2020 09:26:40: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:26:40: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:26:40: #1 total tags in treatment: 36622629 INFO @ Tue, 16 Jun 2020 09:26:40: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:26:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:26:40: #1 tags after filtering in treatment: 36622629 INFO @ Tue, 16 Jun 2020 09:26:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:26:40: #1 finished! INFO @ Tue, 16 Jun 2020 09:26:40: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:26:40: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:26:41: 33000000 INFO @ Tue, 16 Jun 2020 09:26:43: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:26:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:26:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX466564/SRX466564.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX466564/SRX466564.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX466564/SRX466564.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX466564/SRX466564.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:26:46: 30000000 INFO @ Tue, 16 Jun 2020 09:26:49: 34000000 INFO @ Tue, 16 Jun 2020 09:26:54: 31000000 INFO @ Tue, 16 Jun 2020 09:26:57: 35000000 INFO @ Tue, 16 Jun 2020 09:27:02: 32000000 INFO @ Tue, 16 Jun 2020 09:27:05: 36000000 INFO @ Tue, 16 Jun 2020 09:27:10: 33000000 INFO @ Tue, 16 Jun 2020 09:27:10: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:27:10: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:27:10: #1 total tags in treatment: 36622629 INFO @ Tue, 16 Jun 2020 09:27:10: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:27:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:27:10: #1 tags after filtering in treatment: 36622629 INFO @ Tue, 16 Jun 2020 09:27:10: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:27:10: #1 finished! INFO @ Tue, 16 Jun 2020 09:27:10: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:27:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:27:13: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:27:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:27:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX466564/SRX466564.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX466564/SRX466564.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX466564/SRX466564.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX466564/SRX466564.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:27:17: 34000000 INFO @ Tue, 16 Jun 2020 09:27:24: 35000000 INFO @ Tue, 16 Jun 2020 09:27:31: 36000000 INFO @ Tue, 16 Jun 2020 09:27:36: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:27:36: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:27:36: #1 total tags in treatment: 36622629 INFO @ Tue, 16 Jun 2020 09:27:36: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:27:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:27:37: #1 tags after filtering in treatment: 36622629 INFO @ Tue, 16 Jun 2020 09:27:37: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:27:37: #1 finished! INFO @ Tue, 16 Jun 2020 09:27:37: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:27:37: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:27:39: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:27:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:27:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX466564/SRX466564.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX466564/SRX466564.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX466564/SRX466564.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX466564/SRX466564.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling