Job ID = 6368027 SRX = SRX466490 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:49:18 prefetch.2.10.7: 1) Downloading 'SRR1163556'... 2020-06-15T23:49:18 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:54:13 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:54:13 prefetch.2.10.7: 1) 'SRR1163556' was downloaded successfully Read 31312856 spots for SRR1163556/SRR1163556.sra Written 31312856 spots for SRR1163556/SRR1163556.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:06 31312856 reads; of these: 31312856 (100.00%) were unpaired; of these: 662799 (2.12%) aligned 0 times 27563841 (88.03%) aligned exactly 1 time 3086216 (9.86%) aligned >1 times 97.88% overall alignment rate Time searching: 00:07:06 Overall time: 00:07:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 9421044 / 30650057 = 0.3074 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:10:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX466490/SRX466490.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX466490/SRX466490.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX466490/SRX466490.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX466490/SRX466490.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:10:09: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:10:09: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:10:14: 1000000 INFO @ Tue, 16 Jun 2020 09:10:20: 2000000 INFO @ Tue, 16 Jun 2020 09:10:25: 3000000 INFO @ Tue, 16 Jun 2020 09:10:31: 4000000 INFO @ Tue, 16 Jun 2020 09:10:36: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:10:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX466490/SRX466490.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX466490/SRX466490.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX466490/SRX466490.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX466490/SRX466490.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:10:39: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:10:39: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:10:42: 6000000 INFO @ Tue, 16 Jun 2020 09:10:45: 1000000 INFO @ Tue, 16 Jun 2020 09:10:48: 7000000 INFO @ Tue, 16 Jun 2020 09:10:51: 2000000 INFO @ Tue, 16 Jun 2020 09:10:54: 8000000 INFO @ Tue, 16 Jun 2020 09:10:57: 3000000 INFO @ Tue, 16 Jun 2020 09:11:01: 9000000 INFO @ Tue, 16 Jun 2020 09:11:03: 4000000 INFO @ Tue, 16 Jun 2020 09:11:07: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:11:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX466490/SRX466490.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX466490/SRX466490.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX466490/SRX466490.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX466490/SRX466490.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:11:09: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:11:09: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:11:09: 5000000 INFO @ Tue, 16 Jun 2020 09:11:13: 11000000 INFO @ Tue, 16 Jun 2020 09:11:15: 1000000 INFO @ Tue, 16 Jun 2020 09:11:16: 6000000 INFO @ Tue, 16 Jun 2020 09:11:19: 12000000 INFO @ Tue, 16 Jun 2020 09:11:21: 2000000 INFO @ Tue, 16 Jun 2020 09:11:22: 7000000 INFO @ Tue, 16 Jun 2020 09:11:25: 13000000 INFO @ Tue, 16 Jun 2020 09:11:27: 3000000 INFO @ Tue, 16 Jun 2020 09:11:28: 8000000 INFO @ Tue, 16 Jun 2020 09:11:32: 14000000 INFO @ Tue, 16 Jun 2020 09:11:34: 4000000 INFO @ Tue, 16 Jun 2020 09:11:34: 9000000 INFO @ Tue, 16 Jun 2020 09:11:38: 15000000 INFO @ Tue, 16 Jun 2020 09:11:40: 5000000 INFO @ Tue, 16 Jun 2020 09:11:41: 10000000 INFO @ Tue, 16 Jun 2020 09:11:44: 16000000 INFO @ Tue, 16 Jun 2020 09:11:46: 6000000 INFO @ Tue, 16 Jun 2020 09:11:47: 11000000 INFO @ Tue, 16 Jun 2020 09:11:50: 17000000 INFO @ Tue, 16 Jun 2020 09:11:52: 7000000 INFO @ Tue, 16 Jun 2020 09:11:53: 12000000 INFO @ Tue, 16 Jun 2020 09:11:56: 18000000 INFO @ Tue, 16 Jun 2020 09:11:58: 8000000 INFO @ Tue, 16 Jun 2020 09:11:59: 13000000 INFO @ Tue, 16 Jun 2020 09:12:03: 19000000 INFO @ Tue, 16 Jun 2020 09:12:04: 9000000 INFO @ Tue, 16 Jun 2020 09:12:05: 14000000 INFO @ Tue, 16 Jun 2020 09:12:09: 20000000 INFO @ Tue, 16 Jun 2020 09:12:11: 10000000 INFO @ Tue, 16 Jun 2020 09:12:11: 15000000 INFO @ Tue, 16 Jun 2020 09:12:15: 21000000 INFO @ Tue, 16 Jun 2020 09:12:17: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:12:17: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:12:17: #1 total tags in treatment: 21229013 INFO @ Tue, 16 Jun 2020 09:12:17: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:12:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:12:17: 11000000 INFO @ Tue, 16 Jun 2020 09:12:17: #1 tags after filtering in treatment: 21229013 INFO @ Tue, 16 Jun 2020 09:12:17: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:12:17: #1 finished! INFO @ Tue, 16 Jun 2020 09:12:17: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:12:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:12:17: 16000000 INFO @ Tue, 16 Jun 2020 09:12:18: #2 number of paired peaks: 21 WARNING @ Tue, 16 Jun 2020 09:12:18: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:12:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX466490/SRX466490.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX466490/SRX466490.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX466490/SRX466490.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX466490/SRX466490.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:12:23: 12000000 INFO @ Tue, 16 Jun 2020 09:12:24: 17000000 INFO @ Tue, 16 Jun 2020 09:12:29: 13000000 INFO @ Tue, 16 Jun 2020 09:12:30: 18000000 INFO @ Tue, 16 Jun 2020 09:12:35: 14000000 INFO @ Tue, 16 Jun 2020 09:12:36: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:12:41: 15000000 INFO @ Tue, 16 Jun 2020 09:12:42: 20000000 INFO @ Tue, 16 Jun 2020 09:12:47: 16000000 INFO @ Tue, 16 Jun 2020 09:12:48: 21000000 INFO @ Tue, 16 Jun 2020 09:12:50: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:12:50: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:12:50: #1 total tags in treatment: 21229013 INFO @ Tue, 16 Jun 2020 09:12:50: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:12:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:12:50: #1 tags after filtering in treatment: 21229013 INFO @ Tue, 16 Jun 2020 09:12:50: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:12:50: #1 finished! INFO @ Tue, 16 Jun 2020 09:12:50: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:12:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:12:52: #2 number of paired peaks: 21 WARNING @ Tue, 16 Jun 2020 09:12:52: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:12:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX466490/SRX466490.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX466490/SRX466490.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX466490/SRX466490.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX466490/SRX466490.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:12:53: 17000000 INFO @ Tue, 16 Jun 2020 09:12:59: 18000000 INFO @ Tue, 16 Jun 2020 09:13:05: 19000000 INFO @ Tue, 16 Jun 2020 09:13:11: 20000000 INFO @ Tue, 16 Jun 2020 09:13:17: 21000000 INFO @ Tue, 16 Jun 2020 09:13:19: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:13:19: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:13:19: #1 total tags in treatment: 21229013 INFO @ Tue, 16 Jun 2020 09:13:19: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:13:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:13:19: #1 tags after filtering in treatment: 21229013 INFO @ Tue, 16 Jun 2020 09:13:19: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:13:19: #1 finished! INFO @ Tue, 16 Jun 2020 09:13:19: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:13:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:13:20: #2 number of paired peaks: 21 WARNING @ Tue, 16 Jun 2020 09:13:20: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:13:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX466490/SRX466490.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX466490/SRX466490.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX466490/SRX466490.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX466490/SRX466490.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。