Job ID = 6367991 SRX = SRX463083 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:52:48 prefetch.2.10.7: 1) Downloading 'SRR1159145'... 2020-06-15T23:52:48 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:54:53 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:54:53 prefetch.2.10.7: 1) 'SRR1159145' was downloaded successfully Read 27166289 spots for SRR1159145/SRR1159145.sra Written 27166289 spots for SRR1159145/SRR1159145.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:59 27166289 reads; of these: 27166289 (100.00%) were unpaired; of these: 524030 (1.93%) aligned 0 times 22842781 (84.09%) aligned exactly 1 time 3799478 (13.99%) aligned >1 times 98.07% overall alignment rate Time searching: 00:04:59 Overall time: 00:04:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3872625 / 26642259 = 0.1454 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:07:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX463083/SRX463083.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX463083/SRX463083.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX463083/SRX463083.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX463083/SRX463083.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:07:29: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:07:29: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:07:35: 1000000 INFO @ Tue, 16 Jun 2020 09:07:41: 2000000 INFO @ Tue, 16 Jun 2020 09:07:47: 3000000 INFO @ Tue, 16 Jun 2020 09:07:52: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:07:58: 5000000 INFO @ Tue, 16 Jun 2020 09:07:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX463083/SRX463083.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX463083/SRX463083.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX463083/SRX463083.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX463083/SRX463083.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:07:59: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:07:59: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:08:05: 6000000 INFO @ Tue, 16 Jun 2020 09:08:06: 1000000 INFO @ Tue, 16 Jun 2020 09:08:11: 7000000 INFO @ Tue, 16 Jun 2020 09:08:12: 2000000 INFO @ Tue, 16 Jun 2020 09:08:18: 8000000 INFO @ Tue, 16 Jun 2020 09:08:19: 3000000 INFO @ Tue, 16 Jun 2020 09:08:24: 9000000 INFO @ Tue, 16 Jun 2020 09:08:25: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:08:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX463083/SRX463083.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX463083/SRX463083.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX463083/SRX463083.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX463083/SRX463083.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:08:29: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:08:29: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:08:31: 10000000 INFO @ Tue, 16 Jun 2020 09:08:32: 5000000 INFO @ Tue, 16 Jun 2020 09:08:35: 1000000 INFO @ Tue, 16 Jun 2020 09:08:38: 11000000 INFO @ Tue, 16 Jun 2020 09:08:38: 6000000 INFO @ Tue, 16 Jun 2020 09:08:42: 2000000 INFO @ Tue, 16 Jun 2020 09:08:44: 12000000 INFO @ Tue, 16 Jun 2020 09:08:45: 7000000 INFO @ Tue, 16 Jun 2020 09:08:48: 3000000 INFO @ Tue, 16 Jun 2020 09:08:51: 13000000 INFO @ Tue, 16 Jun 2020 09:08:52: 8000000 INFO @ Tue, 16 Jun 2020 09:08:55: 4000000 INFO @ Tue, 16 Jun 2020 09:08:58: 14000000 INFO @ Tue, 16 Jun 2020 09:08:58: 9000000 INFO @ Tue, 16 Jun 2020 09:09:02: 5000000 INFO @ Tue, 16 Jun 2020 09:09:04: 15000000 INFO @ Tue, 16 Jun 2020 09:09:05: 10000000 INFO @ Tue, 16 Jun 2020 09:09:08: 6000000 INFO @ Tue, 16 Jun 2020 09:09:11: 16000000 INFO @ Tue, 16 Jun 2020 09:09:12: 11000000 INFO @ Tue, 16 Jun 2020 09:09:15: 7000000 INFO @ Tue, 16 Jun 2020 09:09:18: 17000000 INFO @ Tue, 16 Jun 2020 09:09:18: 12000000 INFO @ Tue, 16 Jun 2020 09:09:21: 8000000 INFO @ Tue, 16 Jun 2020 09:09:24: 18000000 INFO @ Tue, 16 Jun 2020 09:09:25: 13000000 INFO @ Tue, 16 Jun 2020 09:09:28: 9000000 INFO @ Tue, 16 Jun 2020 09:09:31: 19000000 INFO @ Tue, 16 Jun 2020 09:09:31: 14000000 INFO @ Tue, 16 Jun 2020 09:09:34: 10000000 INFO @ Tue, 16 Jun 2020 09:09:38: 20000000 INFO @ Tue, 16 Jun 2020 09:09:38: 15000000 INFO @ Tue, 16 Jun 2020 09:09:41: 11000000 INFO @ Tue, 16 Jun 2020 09:09:44: 21000000 INFO @ Tue, 16 Jun 2020 09:09:45: 16000000 INFO @ Tue, 16 Jun 2020 09:09:48: 12000000 INFO @ Tue, 16 Jun 2020 09:09:51: 22000000 INFO @ Tue, 16 Jun 2020 09:09:51: 17000000 INFO @ Tue, 16 Jun 2020 09:09:54: 13000000 INFO @ Tue, 16 Jun 2020 09:09:56: #1 tag size is determined as 36 bps INFO @ Tue, 16 Jun 2020 09:09:56: #1 tag size = 36 INFO @ Tue, 16 Jun 2020 09:09:56: #1 total tags in treatment: 22769634 INFO @ Tue, 16 Jun 2020 09:09:56: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:09:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:09:57: #1 tags after filtering in treatment: 22769634 INFO @ Tue, 16 Jun 2020 09:09:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:09:57: #1 finished! INFO @ Tue, 16 Jun 2020 09:09:57: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:09:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:09:58: 18000000 INFO @ Tue, 16 Jun 2020 09:09:58: #2 number of paired peaks: 92 WARNING @ Tue, 16 Jun 2020 09:09:58: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:09:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX463083/SRX463083.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX463083/SRX463083.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX463083/SRX463083.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX463083/SRX463083.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:10:01: 14000000 INFO @ Tue, 16 Jun 2020 09:10:05: 19000000 INFO @ Tue, 16 Jun 2020 09:10:07: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:10:12: 20000000 INFO @ Tue, 16 Jun 2020 09:10:14: 16000000 INFO @ Tue, 16 Jun 2020 09:10:19: 21000000 INFO @ Tue, 16 Jun 2020 09:10:20: 17000000 INFO @ Tue, 16 Jun 2020 09:10:25: 22000000 INFO @ Tue, 16 Jun 2020 09:10:27: 18000000 INFO @ Tue, 16 Jun 2020 09:10:31: #1 tag size is determined as 36 bps INFO @ Tue, 16 Jun 2020 09:10:31: #1 tag size = 36 INFO @ Tue, 16 Jun 2020 09:10:31: #1 total tags in treatment: 22769634 INFO @ Tue, 16 Jun 2020 09:10:31: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:10:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:10:31: #1 tags after filtering in treatment: 22769634 INFO @ Tue, 16 Jun 2020 09:10:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:10:31: #1 finished! INFO @ Tue, 16 Jun 2020 09:10:31: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:10:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:10:33: #2 number of paired peaks: 92 WARNING @ Tue, 16 Jun 2020 09:10:33: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:10:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX463083/SRX463083.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX463083/SRX463083.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX463083/SRX463083.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX463083/SRX463083.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:10:33: 19000000 INFO @ Tue, 16 Jun 2020 09:10:39: 20000000 INFO @ Tue, 16 Jun 2020 09:10:45: 21000000 INFO @ Tue, 16 Jun 2020 09:10:51: 22000000 INFO @ Tue, 16 Jun 2020 09:10:56: #1 tag size is determined as 36 bps INFO @ Tue, 16 Jun 2020 09:10:56: #1 tag size = 36 INFO @ Tue, 16 Jun 2020 09:10:56: #1 total tags in treatment: 22769634 INFO @ Tue, 16 Jun 2020 09:10:56: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:10:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:10:57: #1 tags after filtering in treatment: 22769634 INFO @ Tue, 16 Jun 2020 09:10:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:10:57: #1 finished! INFO @ Tue, 16 Jun 2020 09:10:57: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:10:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:10:58: #2 number of paired peaks: 92 WARNING @ Tue, 16 Jun 2020 09:10:58: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:10:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX463083/SRX463083.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX463083/SRX463083.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX463083/SRX463083.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX463083/SRX463083.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。