Job ID = 6367961 SRX = SRX4419829 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-06-15T23:43:14 prefetch.2.10.7: 1) Downloading 'SRR7553968'... 2020-06-15T23:43:14 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:47:18 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:47:18 prefetch.2.10.7: 1) 'SRR7553968' was downloaded successfully Read 18809593 spots for SRR7553968/SRR7553968.sra Written 18809593 spots for SRR7553968/SRR7553968.sra 2020-06-15T23:49:23 prefetch.2.10.7: 1) Downloading 'SRR7553969'... 2020-06-15T23:49:23 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:50:33 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:50:33 prefetch.2.10.7: 'SRR7553969' is valid 2020-06-15T23:50:33 prefetch.2.10.7: 1) 'SRR7553969' was downloaded successfully Read 7246577 spots for SRR7553969/SRR7553969.sra Written 7246577 spots for SRR7553969/SRR7553969.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:27:25 26056170 reads; of these: 26056170 (100.00%) were paired; of these: 2768659 (10.63%) aligned concordantly 0 times 19861565 (76.23%) aligned concordantly exactly 1 time 3425946 (13.15%) aligned concordantly >1 times ---- 2768659 pairs aligned concordantly 0 times; of these: 1234726 (44.60%) aligned discordantly 1 time ---- 1533933 pairs aligned 0 times concordantly or discordantly; of these: 3067866 mates make up the pairs; of these: 1759275 (57.35%) aligned 0 times 776110 (25.30%) aligned exactly 1 time 532481 (17.36%) aligned >1 times 96.62% overall alignment rate Time searching: 00:27:25 Overall time: 00:27:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 786297 / 23880066 = 0.0329 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:35:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4419829/SRX4419829.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4419829/SRX4419829.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4419829/SRX4419829.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4419829/SRX4419829.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:35:47: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:35:47: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:35:53: 1000000 INFO @ Tue, 16 Jun 2020 09:35:59: 2000000 INFO @ Tue, 16 Jun 2020 09:36:05: 3000000 INFO @ Tue, 16 Jun 2020 09:36:11: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:36:16: 5000000 INFO @ Tue, 16 Jun 2020 09:36:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4419829/SRX4419829.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4419829/SRX4419829.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4419829/SRX4419829.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4419829/SRX4419829.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:36:17: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:36:17: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:36:23: 6000000 INFO @ Tue, 16 Jun 2020 09:36:24: 1000000 INFO @ Tue, 16 Jun 2020 09:36:29: 7000000 INFO @ Tue, 16 Jun 2020 09:36:30: 2000000 INFO @ Tue, 16 Jun 2020 09:36:35: 8000000 INFO @ Tue, 16 Jun 2020 09:36:37: 3000000 INFO @ Tue, 16 Jun 2020 09:36:41: 9000000 INFO @ Tue, 16 Jun 2020 09:36:43: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:36:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4419829/SRX4419829.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4419829/SRX4419829.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4419829/SRX4419829.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4419829/SRX4419829.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:36:48: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:36:48: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:36:48: 10000000 INFO @ Tue, 16 Jun 2020 09:36:50: 5000000 INFO @ Tue, 16 Jun 2020 09:36:54: 11000000 INFO @ Tue, 16 Jun 2020 09:36:54: 1000000 INFO @ Tue, 16 Jun 2020 09:36:56: 6000000 INFO @ Tue, 16 Jun 2020 09:37:01: 12000000 INFO @ Tue, 16 Jun 2020 09:37:01: 2000000 INFO @ Tue, 16 Jun 2020 09:37:03: 7000000 INFO @ Tue, 16 Jun 2020 09:37:07: 13000000 INFO @ Tue, 16 Jun 2020 09:37:08: 3000000 INFO @ Tue, 16 Jun 2020 09:37:09: 8000000 INFO @ Tue, 16 Jun 2020 09:37:14: 14000000 INFO @ Tue, 16 Jun 2020 09:37:15: 4000000 INFO @ Tue, 16 Jun 2020 09:37:16: 9000000 INFO @ Tue, 16 Jun 2020 09:37:20: 15000000 INFO @ Tue, 16 Jun 2020 09:37:21: 5000000 INFO @ Tue, 16 Jun 2020 09:37:23: 10000000 INFO @ Tue, 16 Jun 2020 09:37:27: 16000000 INFO @ Tue, 16 Jun 2020 09:37:28: 6000000 INFO @ Tue, 16 Jun 2020 09:37:29: 11000000 INFO @ Tue, 16 Jun 2020 09:37:33: 17000000 INFO @ Tue, 16 Jun 2020 09:37:34: 7000000 INFO @ Tue, 16 Jun 2020 09:37:36: 12000000 INFO @ Tue, 16 Jun 2020 09:37:40: 18000000 INFO @ Tue, 16 Jun 2020 09:37:41: 8000000 INFO @ Tue, 16 Jun 2020 09:37:42: 13000000 INFO @ Tue, 16 Jun 2020 09:37:46: 19000000 INFO @ Tue, 16 Jun 2020 09:37:48: 9000000 INFO @ Tue, 16 Jun 2020 09:37:49: 14000000 INFO @ Tue, 16 Jun 2020 09:37:53: 20000000 INFO @ Tue, 16 Jun 2020 09:37:54: 10000000 INFO @ Tue, 16 Jun 2020 09:37:56: 15000000 INFO @ Tue, 16 Jun 2020 09:38:00: 21000000 INFO @ Tue, 16 Jun 2020 09:38:01: 11000000 INFO @ Tue, 16 Jun 2020 09:38:02: 16000000 INFO @ Tue, 16 Jun 2020 09:38:06: 22000000 INFO @ Tue, 16 Jun 2020 09:38:07: 12000000 INFO @ Tue, 16 Jun 2020 09:38:08: 17000000 INFO @ Tue, 16 Jun 2020 09:38:13: 23000000 INFO @ Tue, 16 Jun 2020 09:38:14: 13000000 INFO @ Tue, 16 Jun 2020 09:38:15: 18000000 INFO @ Tue, 16 Jun 2020 09:38:19: 24000000 INFO @ Tue, 16 Jun 2020 09:38:20: 14000000 INFO @ Tue, 16 Jun 2020 09:38:21: 19000000 INFO @ Tue, 16 Jun 2020 09:38:26: 25000000 INFO @ Tue, 16 Jun 2020 09:38:26: 15000000 INFO @ Tue, 16 Jun 2020 09:38:28: 20000000 INFO @ Tue, 16 Jun 2020 09:38:32: 26000000 INFO @ Tue, 16 Jun 2020 09:38:33: 16000000 INFO @ Tue, 16 Jun 2020 09:38:34: 21000000 INFO @ Tue, 16 Jun 2020 09:38:38: 27000000 INFO @ Tue, 16 Jun 2020 09:38:39: 17000000 INFO @ Tue, 16 Jun 2020 09:38:40: 22000000 INFO @ Tue, 16 Jun 2020 09:38:44: 28000000 INFO @ Tue, 16 Jun 2020 09:38:45: 18000000 INFO @ Tue, 16 Jun 2020 09:38:46: 23000000 INFO @ Tue, 16 Jun 2020 09:38:50: 29000000 INFO @ Tue, 16 Jun 2020 09:38:52: 19000000 INFO @ Tue, 16 Jun 2020 09:38:52: 24000000 INFO @ Tue, 16 Jun 2020 09:38:56: 30000000 INFO @ Tue, 16 Jun 2020 09:38:58: 20000000 INFO @ Tue, 16 Jun 2020 09:38:58: 25000000 INFO @ Tue, 16 Jun 2020 09:39:02: 31000000 INFO @ Tue, 16 Jun 2020 09:39:04: 26000000 INFO @ Tue, 16 Jun 2020 09:39:04: 21000000 INFO @ Tue, 16 Jun 2020 09:39:08: 32000000 INFO @ Tue, 16 Jun 2020 09:39:11: 27000000 INFO @ Tue, 16 Jun 2020 09:39:11: 22000000 INFO @ Tue, 16 Jun 2020 09:39:14: 33000000 INFO @ Tue, 16 Jun 2020 09:39:17: 28000000 INFO @ Tue, 16 Jun 2020 09:39:17: 23000000 INFO @ Tue, 16 Jun 2020 09:39:20: 34000000 INFO @ Tue, 16 Jun 2020 09:39:23: 29000000 INFO @ Tue, 16 Jun 2020 09:39:24: 24000000 INFO @ Tue, 16 Jun 2020 09:39:26: 35000000 INFO @ Tue, 16 Jun 2020 09:39:29: 30000000 INFO @ Tue, 16 Jun 2020 09:39:30: 25000000 INFO @ Tue, 16 Jun 2020 09:39:32: 36000000 INFO @ Tue, 16 Jun 2020 09:39:35: 31000000 INFO @ Tue, 16 Jun 2020 09:39:37: 26000000 INFO @ Tue, 16 Jun 2020 09:39:38: 37000000 INFO @ Tue, 16 Jun 2020 09:39:42: 32000000 INFO @ Tue, 16 Jun 2020 09:39:43: 27000000 INFO @ Tue, 16 Jun 2020 09:39:44: 38000000 INFO @ Tue, 16 Jun 2020 09:39:48: 33000000 INFO @ Tue, 16 Jun 2020 09:39:49: 28000000 INFO @ Tue, 16 Jun 2020 09:39:50: 39000000 INFO @ Tue, 16 Jun 2020 09:39:54: 34000000 INFO @ Tue, 16 Jun 2020 09:39:56: 29000000 INFO @ Tue, 16 Jun 2020 09:39:57: 40000000 INFO @ Tue, 16 Jun 2020 09:40:00: 35000000 INFO @ Tue, 16 Jun 2020 09:40:03: 30000000 INFO @ Tue, 16 Jun 2020 09:40:03: 41000000 INFO @ Tue, 16 Jun 2020 09:40:06: 36000000 INFO @ Tue, 16 Jun 2020 09:40:09: 31000000 INFO @ Tue, 16 Jun 2020 09:40:09: 42000000 INFO @ Tue, 16 Jun 2020 09:40:12: 37000000 INFO @ Tue, 16 Jun 2020 09:40:15: 32000000 INFO @ Tue, 16 Jun 2020 09:40:15: 43000000 INFO @ Tue, 16 Jun 2020 09:40:19: 38000000 INFO @ Tue, 16 Jun 2020 09:40:21: 33000000 INFO @ Tue, 16 Jun 2020 09:40:22: 44000000 INFO @ Tue, 16 Jun 2020 09:40:26: 39000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:40:28: 45000000 INFO @ Tue, 16 Jun 2020 09:40:28: 34000000 INFO @ Tue, 16 Jun 2020 09:40:32: 40000000 INFO @ Tue, 16 Jun 2020 09:40:34: 46000000 INFO @ Tue, 16 Jun 2020 09:40:35: 35000000 INFO @ Tue, 16 Jun 2020 09:40:39: 41000000 INFO @ Tue, 16 Jun 2020 09:40:40: 47000000 INFO @ Tue, 16 Jun 2020 09:40:41: 36000000 INFO @ Tue, 16 Jun 2020 09:40:45: 42000000 INFO @ Tue, 16 Jun 2020 09:40:46: 48000000 INFO @ Tue, 16 Jun 2020 09:40:47: 37000000 INFO @ Tue, 16 Jun 2020 09:40:51: 43000000 INFO @ Tue, 16 Jun 2020 09:40:52: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:40:52: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:40:52: #1 total tags in treatment: 22508680 INFO @ Tue, 16 Jun 2020 09:40:52: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:40:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:40:52: #1 tags after filtering in treatment: 18647251 INFO @ Tue, 16 Jun 2020 09:40:52: #1 Redundant rate of treatment: 0.17 INFO @ Tue, 16 Jun 2020 09:40:52: #1 finished! INFO @ Tue, 16 Jun 2020 09:40:52: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:40:52: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:40:54: #2 number of paired peaks: 118 WARNING @ Tue, 16 Jun 2020 09:40:54: Fewer paired peaks (118) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 118 pairs to build model! INFO @ Tue, 16 Jun 2020 09:40:54: start model_add_line... INFO @ Tue, 16 Jun 2020 09:40:54: start X-correlation... INFO @ Tue, 16 Jun 2020 09:40:54: end of X-cor INFO @ Tue, 16 Jun 2020 09:40:54: #2 finished! INFO @ Tue, 16 Jun 2020 09:40:54: #2 predicted fragment length is 63 bps INFO @ Tue, 16 Jun 2020 09:40:54: #2 alternative fragment length(s) may be 4,63,158 bps INFO @ Tue, 16 Jun 2020 09:40:54: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4419829/SRX4419829.05_model.r WARNING @ Tue, 16 Jun 2020 09:40:54: #2 Since the d (63) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 09:40:54: #2 You may need to consider one of the other alternative d(s): 4,63,158 WARNING @ Tue, 16 Jun 2020 09:40:54: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 09:40:54: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:40:54: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:40:54: 38000000 INFO @ Tue, 16 Jun 2020 09:40:57: 44000000 INFO @ Tue, 16 Jun 2020 09:41:00: 39000000 INFO @ Tue, 16 Jun 2020 09:41:03: 45000000 INFO @ Tue, 16 Jun 2020 09:41:07: 40000000 INFO @ Tue, 16 Jun 2020 09:41:08: 46000000 INFO @ Tue, 16 Jun 2020 09:41:13: 41000000 INFO @ Tue, 16 Jun 2020 09:41:14: 47000000 INFO @ Tue, 16 Jun 2020 09:41:19: 48000000 INFO @ Tue, 16 Jun 2020 09:41:20: 42000000 INFO @ Tue, 16 Jun 2020 09:41:23: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 09:41:24: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:41:24: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:41:24: #1 total tags in treatment: 22508680 INFO @ Tue, 16 Jun 2020 09:41:24: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:41:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:41:24: #1 tags after filtering in treatment: 18647251 INFO @ Tue, 16 Jun 2020 09:41:24: #1 Redundant rate of treatment: 0.17 INFO @ Tue, 16 Jun 2020 09:41:24: #1 finished! INFO @ Tue, 16 Jun 2020 09:41:24: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:41:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:41:25: #2 number of paired peaks: 118 WARNING @ Tue, 16 Jun 2020 09:41:25: Fewer paired peaks (118) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 118 pairs to build model! INFO @ Tue, 16 Jun 2020 09:41:25: start model_add_line... INFO @ Tue, 16 Jun 2020 09:41:26: start X-correlation... INFO @ Tue, 16 Jun 2020 09:41:26: end of X-cor INFO @ Tue, 16 Jun 2020 09:41:26: #2 finished! INFO @ Tue, 16 Jun 2020 09:41:26: #2 predicted fragment length is 63 bps INFO @ Tue, 16 Jun 2020 09:41:26: #2 alternative fragment length(s) may be 4,63,158 bps INFO @ Tue, 16 Jun 2020 09:41:26: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4419829/SRX4419829.10_model.r WARNING @ Tue, 16 Jun 2020 09:41:26: #2 Since the d (63) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 09:41:26: #2 You may need to consider one of the other alternative d(s): 4,63,158 WARNING @ Tue, 16 Jun 2020 09:41:26: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 09:41:26: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:41:26: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:41:26: 43000000 INFO @ Tue, 16 Jun 2020 09:41:33: 44000000 INFO @ Tue, 16 Jun 2020 09:41:38: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4419829/SRX4419829.05_peaks.xls INFO @ Tue, 16 Jun 2020 09:41:38: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4419829/SRX4419829.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:41:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4419829/SRX4419829.05_summits.bed INFO @ Tue, 16 Jun 2020 09:41:38: Done! INFO @ Tue, 16 Jun 2020 09:41:39: 45000000 pass1 - making usageList (6 chroms): 2 millis pass2 - checking and writing primary data (460 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:41:45: 46000000 INFO @ Tue, 16 Jun 2020 09:41:51: 47000000 INFO @ Tue, 16 Jun 2020 09:41:55: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:41:57: 48000000 INFO @ Tue, 16 Jun 2020 09:42:02: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:42:02: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:42:02: #1 total tags in treatment: 22508680 INFO @ Tue, 16 Jun 2020 09:42:02: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:42:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:42:03: #1 tags after filtering in treatment: 18647251 INFO @ Tue, 16 Jun 2020 09:42:03: #1 Redundant rate of treatment: 0.17 INFO @ Tue, 16 Jun 2020 09:42:03: #1 finished! INFO @ Tue, 16 Jun 2020 09:42:03: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:42:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:42:04: #2 number of paired peaks: 118 WARNING @ Tue, 16 Jun 2020 09:42:04: Fewer paired peaks (118) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 118 pairs to build model! INFO @ Tue, 16 Jun 2020 09:42:04: start model_add_line... INFO @ Tue, 16 Jun 2020 09:42:04: start X-correlation... INFO @ Tue, 16 Jun 2020 09:42:04: end of X-cor INFO @ Tue, 16 Jun 2020 09:42:04: #2 finished! INFO @ Tue, 16 Jun 2020 09:42:04: #2 predicted fragment length is 63 bps INFO @ Tue, 16 Jun 2020 09:42:04: #2 alternative fragment length(s) may be 4,63,158 bps INFO @ Tue, 16 Jun 2020 09:42:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4419829/SRX4419829.20_model.r WARNING @ Tue, 16 Jun 2020 09:42:04: #2 Since the d (63) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 09:42:04: #2 You may need to consider one of the other alternative d(s): 4,63,158 WARNING @ Tue, 16 Jun 2020 09:42:04: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 09:42:04: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:42:04: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:42:10: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4419829/SRX4419829.10_peaks.xls INFO @ Tue, 16 Jun 2020 09:42:10: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4419829/SRX4419829.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:42:10: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4419829/SRX4419829.10_summits.bed INFO @ Tue, 16 Jun 2020 09:42:10: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (282 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:42:33: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 09:42:48: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4419829/SRX4419829.20_peaks.xls INFO @ Tue, 16 Jun 2020 09:42:48: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4419829/SRX4419829.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:42:48: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4419829/SRX4419829.20_summits.bed INFO @ Tue, 16 Jun 2020 09:42:48: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (102 records, 4 fields): 1 millis CompletedMACS2peakCalling