Job ID = 6367923 SRX = SRX4200532 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:47:29 prefetch.2.10.7: 1) Downloading 'SRR7297998'... 2020-06-15T23:47:29 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:55:18 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:55:18 prefetch.2.10.7: 1) 'SRR7297998' was downloaded successfully Read 38575975 spots for SRR7297998/SRR7297998.sra Written 38575975 spots for SRR7297998/SRR7297998.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:03 38575975 reads; of these: 38575975 (100.00%) were unpaired; of these: 96607 (0.25%) aligned 0 times 33596070 (87.09%) aligned exactly 1 time 4883298 (12.66%) aligned >1 times 99.75% overall alignment rate Time searching: 00:14:03 Overall time: 00:14:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 16760091 / 38479368 = 0.4356 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:21:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4200532/SRX4200532.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4200532/SRX4200532.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4200532/SRX4200532.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4200532/SRX4200532.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:21:24: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:21:24: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:21:31: 1000000 INFO @ Tue, 16 Jun 2020 09:21:38: 2000000 INFO @ Tue, 16 Jun 2020 09:21:45: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:21:52: 4000000 INFO @ Tue, 16 Jun 2020 09:21:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4200532/SRX4200532.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4200532/SRX4200532.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4200532/SRX4200532.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4200532/SRX4200532.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:21:54: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:21:54: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:22:00: 5000000 INFO @ Tue, 16 Jun 2020 09:22:01: 1000000 INFO @ Tue, 16 Jun 2020 09:22:08: 6000000 INFO @ Tue, 16 Jun 2020 09:22:09: 2000000 INFO @ Tue, 16 Jun 2020 09:22:15: 7000000 INFO @ Tue, 16 Jun 2020 09:22:16: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:22:23: 8000000 INFO @ Tue, 16 Jun 2020 09:22:23: 4000000 INFO @ Tue, 16 Jun 2020 09:22:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4200532/SRX4200532.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4200532/SRX4200532.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4200532/SRX4200532.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4200532/SRX4200532.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:22:23: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:22:23: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:22:32: 5000000 INFO @ Tue, 16 Jun 2020 09:22:32: 9000000 INFO @ Tue, 16 Jun 2020 09:22:33: 1000000 INFO @ Tue, 16 Jun 2020 09:22:40: 6000000 INFO @ Tue, 16 Jun 2020 09:22:41: 10000000 INFO @ Tue, 16 Jun 2020 09:22:41: 2000000 INFO @ Tue, 16 Jun 2020 09:22:49: 7000000 INFO @ Tue, 16 Jun 2020 09:22:50: 11000000 INFO @ Tue, 16 Jun 2020 09:22:50: 3000000 INFO @ Tue, 16 Jun 2020 09:22:58: 8000000 INFO @ Tue, 16 Jun 2020 09:22:59: 12000000 INFO @ Tue, 16 Jun 2020 09:23:00: 4000000 INFO @ Tue, 16 Jun 2020 09:23:06: 9000000 INFO @ Tue, 16 Jun 2020 09:23:08: 13000000 INFO @ Tue, 16 Jun 2020 09:23:09: 5000000 INFO @ Tue, 16 Jun 2020 09:23:15: 10000000 INFO @ Tue, 16 Jun 2020 09:23:17: 14000000 INFO @ Tue, 16 Jun 2020 09:23:19: 6000000 INFO @ Tue, 16 Jun 2020 09:23:24: 11000000 INFO @ Tue, 16 Jun 2020 09:23:25: 15000000 INFO @ Tue, 16 Jun 2020 09:23:28: 7000000 INFO @ Tue, 16 Jun 2020 09:23:32: 12000000 INFO @ Tue, 16 Jun 2020 09:23:34: 16000000 INFO @ Tue, 16 Jun 2020 09:23:38: 8000000 INFO @ Tue, 16 Jun 2020 09:23:41: 13000000 INFO @ Tue, 16 Jun 2020 09:23:43: 17000000 INFO @ Tue, 16 Jun 2020 09:23:47: 9000000 INFO @ Tue, 16 Jun 2020 09:23:49: 14000000 INFO @ Tue, 16 Jun 2020 09:23:51: 18000000 INFO @ Tue, 16 Jun 2020 09:23:57: 10000000 INFO @ Tue, 16 Jun 2020 09:23:58: 15000000 INFO @ Tue, 16 Jun 2020 09:24:00: 19000000 INFO @ Tue, 16 Jun 2020 09:24:06: 11000000 INFO @ Tue, 16 Jun 2020 09:24:07: 16000000 INFO @ Tue, 16 Jun 2020 09:24:09: 20000000 INFO @ Tue, 16 Jun 2020 09:24:16: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:24:16: 12000000 INFO @ Tue, 16 Jun 2020 09:24:18: 21000000 INFO @ Tue, 16 Jun 2020 09:24:24: #1 tag size is determined as 75 bps INFO @ Tue, 16 Jun 2020 09:24:24: #1 tag size = 75 INFO @ Tue, 16 Jun 2020 09:24:24: #1 total tags in treatment: 21719277 INFO @ Tue, 16 Jun 2020 09:24:24: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:24:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:24:24: 18000000 INFO @ Tue, 16 Jun 2020 09:24:25: #1 tags after filtering in treatment: 21719277 INFO @ Tue, 16 Jun 2020 09:24:25: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:24:25: #1 finished! INFO @ Tue, 16 Jun 2020 09:24:25: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:24:25: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:24:26: #2 number of paired peaks: 7 WARNING @ Tue, 16 Jun 2020 09:24:26: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:24:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX4200532/SRX4200532.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200532/SRX4200532.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200532/SRX4200532.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200532/SRX4200532.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:24:26: 13000000 INFO @ Tue, 16 Jun 2020 09:24:33: 19000000 INFO @ Tue, 16 Jun 2020 09:24:36: 14000000 INFO @ Tue, 16 Jun 2020 09:24:42: 20000000 INFO @ Tue, 16 Jun 2020 09:24:45: 15000000 INFO @ Tue, 16 Jun 2020 09:24:51: 21000000 INFO @ Tue, 16 Jun 2020 09:24:55: 16000000 INFO @ Tue, 16 Jun 2020 09:24:57: #1 tag size is determined as 75 bps INFO @ Tue, 16 Jun 2020 09:24:57: #1 tag size = 75 INFO @ Tue, 16 Jun 2020 09:24:57: #1 total tags in treatment: 21719277 INFO @ Tue, 16 Jun 2020 09:24:57: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:24:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:24:58: #1 tags after filtering in treatment: 21719277 INFO @ Tue, 16 Jun 2020 09:24:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:24:58: #1 finished! INFO @ Tue, 16 Jun 2020 09:24:58: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:24:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:24:59: #2 number of paired peaks: 7 WARNING @ Tue, 16 Jun 2020 09:24:59: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:24:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX4200532/SRX4200532.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200532/SRX4200532.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200532/SRX4200532.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200532/SRX4200532.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:25:04: 17000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:25:13: 18000000 INFO @ Tue, 16 Jun 2020 09:25:21: 19000000 INFO @ Tue, 16 Jun 2020 09:25:30: 20000000 INFO @ Tue, 16 Jun 2020 09:25:38: 21000000 INFO @ Tue, 16 Jun 2020 09:25:44: #1 tag size is determined as 75 bps INFO @ Tue, 16 Jun 2020 09:25:44: #1 tag size = 75 INFO @ Tue, 16 Jun 2020 09:25:44: #1 total tags in treatment: 21719277 INFO @ Tue, 16 Jun 2020 09:25:44: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:25:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:25:45: #1 tags after filtering in treatment: 21719277 INFO @ Tue, 16 Jun 2020 09:25:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:25:45: #1 finished! INFO @ Tue, 16 Jun 2020 09:25:45: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:25:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:25:46: #2 number of paired peaks: 7 WARNING @ Tue, 16 Jun 2020 09:25:46: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:25:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX4200532/SRX4200532.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200532/SRX4200532.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200532/SRX4200532.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200532/SRX4200532.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling