Job ID = 6367892 SRX = SRX4107875 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:56:12 prefetch.2.10.7: 1) Downloading 'SRR7191151'... 2020-06-15T23:56:12 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:57:09 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:57:09 prefetch.2.10.7: 'SRR7191151' is valid 2020-06-15T23:57:09 prefetch.2.10.7: 1) 'SRR7191151' was downloaded successfully 2020-06-15T23:58:30 prefetch.2.10.7: 'SRR7191151' has 16 unresolved dependencies 2020-06-15T23:58:30 prefetch.2.10.7: 2) Downloading 'ncbi-acc:BX284601.5?vdb-ctx=refseq'... 2020-06-15T23:58:30 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:58:46 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:58:46 prefetch.2.10.7: 2) 'ncbi-acc:BX284601.5?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:58:46 prefetch.2.10.7: 3) Downloading 'ncbi-acc:BX284602.5?vdb-ctx=refseq'... 2020-06-15T23:58:46 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:59:01 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:59:01 prefetch.2.10.7: 3) 'ncbi-acc:BX284602.5?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:59:01 prefetch.2.10.7: 4) Downloading 'ncbi-acc:BX284603.4?vdb-ctx=refseq'... 2020-06-15T23:59:01 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:59:17 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:59:17 prefetch.2.10.7: 4) 'ncbi-acc:BX284603.4?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:59:17 prefetch.2.10.7: 5) Downloading 'ncbi-acc:BX284604.4?vdb-ctx=refseq'... 2020-06-15T23:59:17 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:59:34 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:59:34 prefetch.2.10.7: 5) 'ncbi-acc:BX284604.4?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:59:34 prefetch.2.10.7: 6) Downloading 'ncbi-acc:BX284605.5?vdb-ctx=refseq'... 2020-06-15T23:59:34 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:59:49 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:59:49 prefetch.2.10.7: 6) 'ncbi-acc:BX284605.5?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:59:49 prefetch.2.10.7: 7) Downloading 'ncbi-acc:BX284606.5?vdb-ctx=refseq'... 2020-06-15T23:59:49 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:00:07 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:00:07 prefetch.2.10.7: 7) 'ncbi-acc:BX284606.5?vdb-ctx=refseq' was downloaded successfully 2020-06-16T00:00:07 prefetch.2.10.7: 8) Downloading 'ncbi-acc:NC_013486.1?vdb-ctx=refseq'... 2020-06-16T00:00:07 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:00:23 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:00:23 prefetch.2.10.7: 8) 'ncbi-acc:NC_013486.1?vdb-ctx=refseq' was downloaded successfully 2020-06-16T00:00:23 prefetch.2.10.7: 9) Downloading 'ncbi-acc:NC_013487.1?vdb-ctx=refseq'... 2020-06-16T00:00:23 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:00:39 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:00:39 prefetch.2.10.7: 9) 'ncbi-acc:NC_013487.1?vdb-ctx=refseq' was downloaded successfully 2020-06-16T00:00:39 prefetch.2.10.7: 10) Downloading 'ncbi-acc:NC_013488.1?vdb-ctx=refseq'... 2020-06-16T00:00:39 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:00:55 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:00:55 prefetch.2.10.7: 10) 'ncbi-acc:NC_013488.1?vdb-ctx=refseq' was downloaded successfully 2020-06-16T00:00:55 prefetch.2.10.7: 11) Downloading 'ncbi-acc:NC_013489.1?vdb-ctx=refseq'... 2020-06-16T00:00:55 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:01:12 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:01:12 prefetch.2.10.7: 11) 'ncbi-acc:NC_013489.1?vdb-ctx=refseq' was downloaded successfully 2020-06-16T00:01:12 prefetch.2.10.7: 12) Downloading 'ncbi-acc:NC_013490.1?vdb-ctx=refseq'... 2020-06-16T00:01:12 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:01:26 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:01:26 prefetch.2.10.7: 12) 'ncbi-acc:NC_013490.1?vdb-ctx=refseq' was downloaded successfully 2020-06-16T00:01:26 prefetch.2.10.7: 13) Downloading 'ncbi-acc:NC_013491.1?vdb-ctx=refseq'... 2020-06-16T00:01:26 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:01:44 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:01:44 prefetch.2.10.7: 13) 'ncbi-acc:NC_013491.1?vdb-ctx=refseq' was downloaded successfully 2020-06-16T00:01:44 prefetch.2.10.7: 14) Downloading 'ncbi-acc:NW_003101683.1?vdb-ctx=refseq'... 2020-06-16T00:01:44 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:01:57 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:01:57 prefetch.2.10.7: 14) 'ncbi-acc:NW_003101683.1?vdb-ctx=refseq' was downloaded successfully 2020-06-16T00:01:57 prefetch.2.10.7: 15) Downloading 'ncbi-acc:NW_003101684.1?vdb-ctx=refseq'... 2020-06-16T00:01:57 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:02:10 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:02:10 prefetch.2.10.7: 15) 'ncbi-acc:NW_003101684.1?vdb-ctx=refseq' was downloaded successfully 2020-06-16T00:02:10 prefetch.2.10.7: 16) Downloading 'ncbi-acc:NW_003101687.1?vdb-ctx=refseq'... 2020-06-16T00:02:10 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:02:22 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:02:22 prefetch.2.10.7: 16) 'ncbi-acc:NW_003101687.1?vdb-ctx=refseq' was downloaded successfully 2020-06-16T00:02:22 prefetch.2.10.7: 17) Downloading 'ncbi-acc:NW_003101688.1?vdb-ctx=refseq'... 2020-06-16T00:02:22 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:02:36 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:02:36 prefetch.2.10.7: 17) 'ncbi-acc:NW_003101688.1?vdb-ctx=refseq' was downloaded successfully Read 19402523 spots for SRR7191151/SRR7191151.sra Written 19402523 spots for SRR7191151/SRR7191151.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:21 19402523 reads; of these: 19402523 (100.00%) were unpaired; of these: 969804 (5.00%) aligned 0 times 15644977 (80.63%) aligned exactly 1 time 2787742 (14.37%) aligned >1 times 95.00% overall alignment rate Time searching: 00:03:21 Overall time: 00:03:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2551461 / 18432719 = 0.1384 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:11:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4107875/SRX4107875.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4107875/SRX4107875.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4107875/SRX4107875.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4107875/SRX4107875.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:11:40: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:11:40: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:11:46: 1000000 INFO @ Tue, 16 Jun 2020 09:11:52: 2000000 INFO @ Tue, 16 Jun 2020 09:11:57: 3000000 INFO @ Tue, 16 Jun 2020 09:12:03: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:12:09: 5000000 INFO @ Tue, 16 Jun 2020 09:12:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4107875/SRX4107875.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4107875/SRX4107875.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4107875/SRX4107875.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4107875/SRX4107875.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:12:09: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:12:09: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:12:16: 6000000 INFO @ Tue, 16 Jun 2020 09:12:17: 1000000 INFO @ Tue, 16 Jun 2020 09:12:23: 7000000 INFO @ Tue, 16 Jun 2020 09:12:25: 2000000 INFO @ Tue, 16 Jun 2020 09:12:31: 8000000 INFO @ Tue, 16 Jun 2020 09:12:33: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:12:38: 9000000 INFO @ Tue, 16 Jun 2020 09:12:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4107875/SRX4107875.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4107875/SRX4107875.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4107875/SRX4107875.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4107875/SRX4107875.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:12:40: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:12:40: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:12:41: 4000000 INFO @ Tue, 16 Jun 2020 09:12:45: 10000000 INFO @ Tue, 16 Jun 2020 09:12:48: 1000000 INFO @ Tue, 16 Jun 2020 09:12:49: 5000000 INFO @ Tue, 16 Jun 2020 09:12:53: 11000000 INFO @ Tue, 16 Jun 2020 09:12:56: 2000000 INFO @ Tue, 16 Jun 2020 09:12:58: 6000000 INFO @ Tue, 16 Jun 2020 09:13:00: 12000000 INFO @ Tue, 16 Jun 2020 09:13:04: 3000000 INFO @ Tue, 16 Jun 2020 09:13:06: 7000000 INFO @ Tue, 16 Jun 2020 09:13:07: 13000000 INFO @ Tue, 16 Jun 2020 09:13:12: 4000000 INFO @ Tue, 16 Jun 2020 09:13:14: 8000000 INFO @ Tue, 16 Jun 2020 09:13:15: 14000000 INFO @ Tue, 16 Jun 2020 09:13:20: 5000000 INFO @ Tue, 16 Jun 2020 09:13:22: 15000000 INFO @ Tue, 16 Jun 2020 09:13:22: 9000000 INFO @ Tue, 16 Jun 2020 09:13:28: 6000000 INFO @ Tue, 16 Jun 2020 09:13:28: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:13:28: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:13:28: #1 total tags in treatment: 15881258 INFO @ Tue, 16 Jun 2020 09:13:28: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:13:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:13:29: #1 tags after filtering in treatment: 15881258 INFO @ Tue, 16 Jun 2020 09:13:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:13:29: #1 finished! INFO @ Tue, 16 Jun 2020 09:13:29: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:13:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:13:30: #2 number of paired peaks: 277 WARNING @ Tue, 16 Jun 2020 09:13:30: Fewer paired peaks (277) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 277 pairs to build model! INFO @ Tue, 16 Jun 2020 09:13:30: start model_add_line... INFO @ Tue, 16 Jun 2020 09:13:30: start X-correlation... INFO @ Tue, 16 Jun 2020 09:13:30: end of X-cor INFO @ Tue, 16 Jun 2020 09:13:30: #2 finished! INFO @ Tue, 16 Jun 2020 09:13:30: #2 predicted fragment length is 77 bps INFO @ Tue, 16 Jun 2020 09:13:30: #2 alternative fragment length(s) may be 4,77 bps INFO @ Tue, 16 Jun 2020 09:13:30: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4107875/SRX4107875.05_model.r WARNING @ Tue, 16 Jun 2020 09:13:30: #2 Since the d (77) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 09:13:30: #2 You may need to consider one of the other alternative d(s): 4,77 WARNING @ Tue, 16 Jun 2020 09:13:30: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 09:13:30: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:13:30: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:13:30: 10000000 INFO @ Tue, 16 Jun 2020 09:13:36: 7000000 INFO @ Tue, 16 Jun 2020 09:13:39: 11000000 INFO @ Tue, 16 Jun 2020 09:13:44: 8000000 INFO @ Tue, 16 Jun 2020 09:13:47: 12000000 INFO @ Tue, 16 Jun 2020 09:13:52: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:13:55: 13000000 INFO @ Tue, 16 Jun 2020 09:14:00: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 09:14:00: 10000000 INFO @ Tue, 16 Jun 2020 09:14:02: 14000000 INFO @ Tue, 16 Jun 2020 09:14:08: 11000000 INFO @ Tue, 16 Jun 2020 09:14:10: 15000000 INFO @ Tue, 16 Jun 2020 09:14:16: 12000000 INFO @ Tue, 16 Jun 2020 09:14:16: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4107875/SRX4107875.05_peaks.xls INFO @ Tue, 16 Jun 2020 09:14:16: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4107875/SRX4107875.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:14:16: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4107875/SRX4107875.05_summits.bed INFO @ Tue, 16 Jun 2020 09:14:16: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (7148 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:14:17: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:14:17: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:14:17: #1 total tags in treatment: 15881258 INFO @ Tue, 16 Jun 2020 09:14:17: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:14:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:14:17: #1 tags after filtering in treatment: 15881258 INFO @ Tue, 16 Jun 2020 09:14:17: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:14:17: #1 finished! INFO @ Tue, 16 Jun 2020 09:14:17: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:14:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:14:18: #2 number of paired peaks: 277 WARNING @ Tue, 16 Jun 2020 09:14:18: Fewer paired peaks (277) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 277 pairs to build model! INFO @ Tue, 16 Jun 2020 09:14:18: start model_add_line... INFO @ Tue, 16 Jun 2020 09:14:18: start X-correlation... INFO @ Tue, 16 Jun 2020 09:14:18: end of X-cor INFO @ Tue, 16 Jun 2020 09:14:18: #2 finished! INFO @ Tue, 16 Jun 2020 09:14:18: #2 predicted fragment length is 77 bps INFO @ Tue, 16 Jun 2020 09:14:18: #2 alternative fragment length(s) may be 4,77 bps INFO @ Tue, 16 Jun 2020 09:14:18: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4107875/SRX4107875.10_model.r WARNING @ Tue, 16 Jun 2020 09:14:18: #2 Since the d (77) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 09:14:18: #2 You may need to consider one of the other alternative d(s): 4,77 WARNING @ Tue, 16 Jun 2020 09:14:18: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 09:14:18: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:14:18: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:14:23: 13000000 INFO @ Tue, 16 Jun 2020 09:14:30: 14000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:14:37: 15000000 INFO @ Tue, 16 Jun 2020 09:14:43: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:14:43: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:14:43: #1 total tags in treatment: 15881258 INFO @ Tue, 16 Jun 2020 09:14:43: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:14:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:14:43: #1 tags after filtering in treatment: 15881258 INFO @ Tue, 16 Jun 2020 09:14:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:14:43: #1 finished! INFO @ Tue, 16 Jun 2020 09:14:43: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:14:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:14:44: #2 number of paired peaks: 277 WARNING @ Tue, 16 Jun 2020 09:14:44: Fewer paired peaks (277) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 277 pairs to build model! INFO @ Tue, 16 Jun 2020 09:14:44: start model_add_line... INFO @ Tue, 16 Jun 2020 09:14:44: start X-correlation... INFO @ Tue, 16 Jun 2020 09:14:44: end of X-cor INFO @ Tue, 16 Jun 2020 09:14:44: #2 finished! INFO @ Tue, 16 Jun 2020 09:14:44: #2 predicted fragment length is 77 bps INFO @ Tue, 16 Jun 2020 09:14:44: #2 alternative fragment length(s) may be 4,77 bps INFO @ Tue, 16 Jun 2020 09:14:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4107875/SRX4107875.20_model.r WARNING @ Tue, 16 Jun 2020 09:14:44: #2 Since the d (77) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 09:14:44: #2 You may need to consider one of the other alternative d(s): 4,77 WARNING @ Tue, 16 Jun 2020 09:14:44: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 09:14:44: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:14:44: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:14:46: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 09:15:01: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4107875/SRX4107875.10_peaks.xls INFO @ Tue, 16 Jun 2020 09:15:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4107875/SRX4107875.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:15:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4107875/SRX4107875.10_summits.bed INFO @ Tue, 16 Jun 2020 09:15:01: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (3841 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:15:13: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 09:15:28: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4107875/SRX4107875.20_peaks.xls INFO @ Tue, 16 Jun 2020 09:15:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4107875/SRX4107875.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:15:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4107875/SRX4107875.20_summits.bed INFO @ Tue, 16 Jun 2020 09:15:28: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1442 records, 4 fields): 3 millis CompletedMACS2peakCalling