Job ID = 6367889 SRX = SRX4107872 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:36:59 prefetch.2.10.7: 1) Downloading 'SRR7191148'... 2020-06-15T23:36:59 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:37:55 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:37:55 prefetch.2.10.7: 'SRR7191148' is valid 2020-06-15T23:37:55 prefetch.2.10.7: 1) 'SRR7191148' was downloaded successfully 2020-06-15T23:39:15 prefetch.2.10.7: 'SRR7191148' has 16 unresolved dependencies 2020-06-15T23:39:15 prefetch.2.10.7: 2) Downloading 'ncbi-acc:BX284601.5?vdb-ctx=refseq'... 2020-06-15T23:39:15 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:39:30 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:39:30 prefetch.2.10.7: 2) 'ncbi-acc:BX284601.5?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:39:30 prefetch.2.10.7: 3) Downloading 'ncbi-acc:BX284602.5?vdb-ctx=refseq'... 2020-06-15T23:39:30 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:39:47 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:39:47 prefetch.2.10.7: 3) 'ncbi-acc:BX284602.5?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:39:47 prefetch.2.10.7: 4) Downloading 'ncbi-acc:BX284603.4?vdb-ctx=refseq'... 2020-06-15T23:39:47 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:40:04 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:40:04 prefetch.2.10.7: 4) 'ncbi-acc:BX284603.4?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:40:04 prefetch.2.10.7: 5) Downloading 'ncbi-acc:BX284604.4?vdb-ctx=refseq'... 2020-06-15T23:40:04 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:40:21 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:40:21 prefetch.2.10.7: 5) 'ncbi-acc:BX284604.4?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:40:21 prefetch.2.10.7: 6) Downloading 'ncbi-acc:BX284605.5?vdb-ctx=refseq'... 2020-06-15T23:40:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:40:41 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:40:41 prefetch.2.10.7: 6) 'ncbi-acc:BX284605.5?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:40:41 prefetch.2.10.7: 7) Downloading 'ncbi-acc:BX284606.5?vdb-ctx=refseq'... 2020-06-15T23:40:41 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:40:57 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:40:57 prefetch.2.10.7: 7) 'ncbi-acc:BX284606.5?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:40:57 prefetch.2.10.7: 8) Downloading 'ncbi-acc:NC_013486.1?vdb-ctx=refseq'... 2020-06-15T23:40:57 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:41:15 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:41:15 prefetch.2.10.7: 8) 'ncbi-acc:NC_013486.1?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:41:15 prefetch.2.10.7: 9) Downloading 'ncbi-acc:NC_013487.1?vdb-ctx=refseq'... 2020-06-15T23:41:15 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:41:32 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:41:32 prefetch.2.10.7: 9) 'ncbi-acc:NC_013487.1?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:41:32 prefetch.2.10.7: 10) Downloading 'ncbi-acc:NC_013488.1?vdb-ctx=refseq'... 2020-06-15T23:41:32 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:41:49 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:41:49 prefetch.2.10.7: 10) 'ncbi-acc:NC_013488.1?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:41:49 prefetch.2.10.7: 11) Downloading 'ncbi-acc:NC_013489.1?vdb-ctx=refseq'... 2020-06-15T23:41:49 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:42:06 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:42:06 prefetch.2.10.7: 11) 'ncbi-acc:NC_013489.1?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:42:06 prefetch.2.10.7: 12) Downloading 'ncbi-acc:NC_013490.1?vdb-ctx=refseq'... 2020-06-15T23:42:06 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:42:22 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:42:22 prefetch.2.10.7: 12) 'ncbi-acc:NC_013490.1?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:42:22 prefetch.2.10.7: 13) Downloading 'ncbi-acc:NC_013491.1?vdb-ctx=refseq'... 2020-06-15T23:42:22 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:42:39 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:42:39 prefetch.2.10.7: 13) 'ncbi-acc:NC_013491.1?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:42:39 prefetch.2.10.7: 14) Downloading 'ncbi-acc:NW_003101683.1?vdb-ctx=refseq'... 2020-06-15T23:42:39 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:42:52 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:42:52 prefetch.2.10.7: 14) 'ncbi-acc:NW_003101683.1?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:42:52 prefetch.2.10.7: 15) Downloading 'ncbi-acc:NW_003101684.1?vdb-ctx=refseq'... 2020-06-15T23:42:52 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:43:05 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:43:05 prefetch.2.10.7: 15) 'ncbi-acc:NW_003101684.1?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:43:05 prefetch.2.10.7: 16) Downloading 'ncbi-acc:NW_003101687.1?vdb-ctx=refseq'... 2020-06-15T23:43:05 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:43:18 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:43:18 prefetch.2.10.7: 16) 'ncbi-acc:NW_003101687.1?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:43:18 prefetch.2.10.7: 17) Downloading 'ncbi-acc:NW_003101688.1?vdb-ctx=refseq'... 2020-06-15T23:43:18 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:43:32 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:43:32 prefetch.2.10.7: 17) 'ncbi-acc:NW_003101688.1?vdb-ctx=refseq' was downloaded successfully Read 19454218 spots for SRR7191148/SRR7191148.sra Written 19454218 spots for SRR7191148/SRR7191148.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:03:11 19454218 reads; of these: 19454218 (100.00%) were unpaired; of these: 1200974 (6.17%) aligned 0 times 15264725 (78.46%) aligned exactly 1 time 2988519 (15.36%) aligned >1 times 93.83% overall alignment rate Time searching: 00:03:12 Overall time: 00:03:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2408261 / 18253244 = 0.1319 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:52:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4107872/SRX4107872.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4107872/SRX4107872.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4107872/SRX4107872.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4107872/SRX4107872.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:52:08: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:52:08: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:52:12: 1000000 INFO @ Tue, 16 Jun 2020 08:52:17: 2000000 INFO @ Tue, 16 Jun 2020 08:52:22: 3000000 INFO @ Tue, 16 Jun 2020 08:52:27: 4000000 INFO @ Tue, 16 Jun 2020 08:52:31: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:52:36: 6000000 INFO @ Tue, 16 Jun 2020 08:52:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4107872/SRX4107872.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4107872/SRX4107872.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4107872/SRX4107872.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4107872/SRX4107872.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:52:38: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:52:38: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:52:41: 7000000 INFO @ Tue, 16 Jun 2020 08:52:43: 1000000 INFO @ Tue, 16 Jun 2020 08:52:46: 8000000 INFO @ Tue, 16 Jun 2020 08:52:48: 2000000 INFO @ Tue, 16 Jun 2020 08:52:51: 9000000 INFO @ Tue, 16 Jun 2020 08:52:53: 3000000 INFO @ Tue, 16 Jun 2020 08:52:56: 10000000 INFO @ Tue, 16 Jun 2020 08:52:58: 4000000 INFO @ Tue, 16 Jun 2020 08:53:02: 11000000 INFO @ Tue, 16 Jun 2020 08:53:03: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:53:07: 12000000 INFO @ Tue, 16 Jun 2020 08:53:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4107872/SRX4107872.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4107872/SRX4107872.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4107872/SRX4107872.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4107872/SRX4107872.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:53:08: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:53:08: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:53:08: 6000000 INFO @ Tue, 16 Jun 2020 08:53:12: 13000000 INFO @ Tue, 16 Jun 2020 08:53:13: 1000000 INFO @ Tue, 16 Jun 2020 08:53:13: 7000000 INFO @ Tue, 16 Jun 2020 08:53:17: 14000000 INFO @ Tue, 16 Jun 2020 08:53:19: 2000000 INFO @ Tue, 16 Jun 2020 08:53:19: 8000000 INFO @ Tue, 16 Jun 2020 08:53:23: 15000000 INFO @ Tue, 16 Jun 2020 08:53:24: 3000000 INFO @ Tue, 16 Jun 2020 08:53:24: 9000000 INFO @ Tue, 16 Jun 2020 08:53:27: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:53:27: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:53:27: #1 total tags in treatment: 15844983 INFO @ Tue, 16 Jun 2020 08:53:27: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:53:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:53:27: #1 tags after filtering in treatment: 15844983 INFO @ Tue, 16 Jun 2020 08:53:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:53:27: #1 finished! INFO @ Tue, 16 Jun 2020 08:53:27: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:53:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:53:28: #2 number of paired peaks: 271 WARNING @ Tue, 16 Jun 2020 08:53:28: Fewer paired peaks (271) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 271 pairs to build model! INFO @ Tue, 16 Jun 2020 08:53:28: start model_add_line... INFO @ Tue, 16 Jun 2020 08:53:29: start X-correlation... INFO @ Tue, 16 Jun 2020 08:53:29: end of X-cor INFO @ Tue, 16 Jun 2020 08:53:29: #2 finished! INFO @ Tue, 16 Jun 2020 08:53:29: #2 predicted fragment length is 52 bps INFO @ Tue, 16 Jun 2020 08:53:29: #2 alternative fragment length(s) may be 4,52 bps INFO @ Tue, 16 Jun 2020 08:53:29: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4107872/SRX4107872.05_model.r WARNING @ Tue, 16 Jun 2020 08:53:29: #2 Since the d (52) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:53:29: #2 You may need to consider one of the other alternative d(s): 4,52 WARNING @ Tue, 16 Jun 2020 08:53:29: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:53:29: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:53:29: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:53:29: 10000000 INFO @ Tue, 16 Jun 2020 08:53:29: 4000000 INFO @ Tue, 16 Jun 2020 08:53:34: 11000000 INFO @ Tue, 16 Jun 2020 08:53:34: 5000000 INFO @ Tue, 16 Jun 2020 08:53:40: 6000000 INFO @ Tue, 16 Jun 2020 08:53:40: 12000000 INFO @ Tue, 16 Jun 2020 08:53:45: 7000000 INFO @ Tue, 16 Jun 2020 08:53:45: 13000000 INFO @ Tue, 16 Jun 2020 08:53:50: 8000000 INFO @ Tue, 16 Jun 2020 08:53:50: 14000000 INFO @ Tue, 16 Jun 2020 08:53:55: 9000000 INFO @ Tue, 16 Jun 2020 08:53:56: 15000000 INFO @ Tue, 16 Jun 2020 08:53:59: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:54:00: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:54:00: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:54:00: #1 total tags in treatment: 15844983 INFO @ Tue, 16 Jun 2020 08:54:00: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:54:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:54:01: #1 tags after filtering in treatment: 15844983 INFO @ Tue, 16 Jun 2020 08:54:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:54:01: #1 finished! INFO @ Tue, 16 Jun 2020 08:54:01: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:54:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:54:01: 10000000 INFO @ Tue, 16 Jun 2020 08:54:02: #2 number of paired peaks: 271 WARNING @ Tue, 16 Jun 2020 08:54:02: Fewer paired peaks (271) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 271 pairs to build model! INFO @ Tue, 16 Jun 2020 08:54:02: start model_add_line... INFO @ Tue, 16 Jun 2020 08:54:02: start X-correlation... INFO @ Tue, 16 Jun 2020 08:54:02: end of X-cor INFO @ Tue, 16 Jun 2020 08:54:02: #2 finished! INFO @ Tue, 16 Jun 2020 08:54:02: #2 predicted fragment length is 52 bps INFO @ Tue, 16 Jun 2020 08:54:02: #2 alternative fragment length(s) may be 4,52 bps INFO @ Tue, 16 Jun 2020 08:54:02: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4107872/SRX4107872.10_model.r WARNING @ Tue, 16 Jun 2020 08:54:02: #2 Since the d (52) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:54:02: #2 You may need to consider one of the other alternative d(s): 4,52 WARNING @ Tue, 16 Jun 2020 08:54:02: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:54:02: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:54:02: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:54:06: 11000000 INFO @ Tue, 16 Jun 2020 08:54:11: 12000000 INFO @ Tue, 16 Jun 2020 08:54:15: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4107872/SRX4107872.05_peaks.xls INFO @ Tue, 16 Jun 2020 08:54:15: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4107872/SRX4107872.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:54:15: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4107872/SRX4107872.05_summits.bed INFO @ Tue, 16 Jun 2020 08:54:15: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (5196 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:54:17: 13000000 INFO @ Tue, 16 Jun 2020 08:54:22: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:54:27: 15000000 INFO @ Tue, 16 Jun 2020 08:54:32: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:54:32: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:54:32: #1 total tags in treatment: 15844983 INFO @ Tue, 16 Jun 2020 08:54:32: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:54:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:54:32: #1 tags after filtering in treatment: 15844983 INFO @ Tue, 16 Jun 2020 08:54:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:54:32: #1 finished! INFO @ Tue, 16 Jun 2020 08:54:32: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:54:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:54:33: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:54:33: #2 number of paired peaks: 271 WARNING @ Tue, 16 Jun 2020 08:54:33: Fewer paired peaks (271) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 271 pairs to build model! INFO @ Tue, 16 Jun 2020 08:54:33: start model_add_line... INFO @ Tue, 16 Jun 2020 08:54:33: start X-correlation... INFO @ Tue, 16 Jun 2020 08:54:33: end of X-cor INFO @ Tue, 16 Jun 2020 08:54:33: #2 finished! INFO @ Tue, 16 Jun 2020 08:54:33: #2 predicted fragment length is 52 bps INFO @ Tue, 16 Jun 2020 08:54:33: #2 alternative fragment length(s) may be 4,52 bps INFO @ Tue, 16 Jun 2020 08:54:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4107872/SRX4107872.20_model.r WARNING @ Tue, 16 Jun 2020 08:54:33: #2 Since the d (52) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:54:33: #2 You may need to consider one of the other alternative d(s): 4,52 WARNING @ Tue, 16 Jun 2020 08:54:33: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:54:33: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:54:33: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:54:50: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4107872/SRX4107872.10_peaks.xls INFO @ Tue, 16 Jun 2020 08:54:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4107872/SRX4107872.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:54:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4107872/SRX4107872.10_summits.bed INFO @ Tue, 16 Jun 2020 08:54:50: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (2550 records, 4 fields): 3 millis CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:55:05: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:55:21: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4107872/SRX4107872.20_peaks.xls INFO @ Tue, 16 Jun 2020 08:55:21: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4107872/SRX4107872.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:55:21: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4107872/SRX4107872.20_summits.bed INFO @ Tue, 16 Jun 2020 08:55:21: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (612 records, 4 fields): 2 millis CompletedMACS2peakCalling