Job ID = 6367832 SRX = SRX4085400 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-06-15T23:37:29 prefetch.2.10.7: 1) Downloading 'SRR7167429'... 2020-06-15T23:37:29 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:54:19 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:54:19 prefetch.2.10.7: 1) 'SRR7167429' was downloaded successfully Read 45810613 spots for SRR7167429/SRR7167429.sra Written 45810613 spots for SRR7167429/SRR7167429.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:32:48 45810613 reads; of these: 45810613 (100.00%) were paired; of these: 2486267 (5.43%) aligned concordantly 0 times 39417445 (86.04%) aligned concordantly exactly 1 time 3906901 (8.53%) aligned concordantly >1 times ---- 2486267 pairs aligned concordantly 0 times; of these: 183141 (7.37%) aligned discordantly 1 time ---- 2303126 pairs aligned 0 times concordantly or discordantly; of these: 4606252 mates make up the pairs; of these: 3280315 (71.21%) aligned 0 times 1065016 (23.12%) aligned exactly 1 time 260921 (5.66%) aligned >1 times 96.42% overall alignment rate Time searching: 00:32:49 Overall time: 00:32:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 19419445 / 43453843 = 0.4469 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:52:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4085400/SRX4085400.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4085400/SRX4085400.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4085400/SRX4085400.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4085400/SRX4085400.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:52:02: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:52:02: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:52:07: 1000000 INFO @ Tue, 16 Jun 2020 09:52:12: 2000000 INFO @ Tue, 16 Jun 2020 09:52:17: 3000000 INFO @ Tue, 16 Jun 2020 09:52:22: 4000000 INFO @ Tue, 16 Jun 2020 09:52:27: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:52:32: 6000000 INFO @ Tue, 16 Jun 2020 09:52:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4085400/SRX4085400.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4085400/SRX4085400.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4085400/SRX4085400.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4085400/SRX4085400.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:52:32: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:52:32: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:52:37: 7000000 INFO @ Tue, 16 Jun 2020 09:52:37: 1000000 INFO @ Tue, 16 Jun 2020 09:52:42: 8000000 INFO @ Tue, 16 Jun 2020 09:52:43: 2000000 INFO @ Tue, 16 Jun 2020 09:52:47: 9000000 INFO @ Tue, 16 Jun 2020 09:52:48: 3000000 INFO @ Tue, 16 Jun 2020 09:52:52: 10000000 INFO @ Tue, 16 Jun 2020 09:52:53: 4000000 INFO @ Tue, 16 Jun 2020 09:52:57: 11000000 INFO @ Tue, 16 Jun 2020 09:52:58: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:53:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4085400/SRX4085400.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4085400/SRX4085400.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4085400/SRX4085400.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4085400/SRX4085400.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:53:02: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:53:02: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:53:02: 12000000 INFO @ Tue, 16 Jun 2020 09:53:03: 6000000 INFO @ Tue, 16 Jun 2020 09:53:08: 13000000 INFO @ Tue, 16 Jun 2020 09:53:08: 1000000 INFO @ Tue, 16 Jun 2020 09:53:08: 7000000 INFO @ Tue, 16 Jun 2020 09:53:13: 14000000 INFO @ Tue, 16 Jun 2020 09:53:14: 8000000 INFO @ Tue, 16 Jun 2020 09:53:14: 2000000 INFO @ Tue, 16 Jun 2020 09:53:18: 15000000 INFO @ Tue, 16 Jun 2020 09:53:19: 9000000 INFO @ Tue, 16 Jun 2020 09:53:20: 3000000 INFO @ Tue, 16 Jun 2020 09:53:23: 16000000 INFO @ Tue, 16 Jun 2020 09:53:24: 10000000 INFO @ Tue, 16 Jun 2020 09:53:26: 4000000 INFO @ Tue, 16 Jun 2020 09:53:29: 17000000 INFO @ Tue, 16 Jun 2020 09:53:29: 11000000 INFO @ Tue, 16 Jun 2020 09:53:32: 5000000 INFO @ Tue, 16 Jun 2020 09:53:34: 18000000 INFO @ Tue, 16 Jun 2020 09:53:35: 12000000 INFO @ Tue, 16 Jun 2020 09:53:38: 6000000 INFO @ Tue, 16 Jun 2020 09:53:39: 19000000 INFO @ Tue, 16 Jun 2020 09:53:40: 13000000 INFO @ Tue, 16 Jun 2020 09:53:44: 7000000 INFO @ Tue, 16 Jun 2020 09:53:44: 20000000 INFO @ Tue, 16 Jun 2020 09:53:45: 14000000 INFO @ Tue, 16 Jun 2020 09:53:49: 21000000 INFO @ Tue, 16 Jun 2020 09:53:50: 8000000 INFO @ Tue, 16 Jun 2020 09:53:51: 15000000 INFO @ Tue, 16 Jun 2020 09:53:55: 22000000 INFO @ Tue, 16 Jun 2020 09:53:56: 9000000 INFO @ Tue, 16 Jun 2020 09:53:56: 16000000 INFO @ Tue, 16 Jun 2020 09:54:00: 23000000 INFO @ Tue, 16 Jun 2020 09:54:01: 17000000 INFO @ Tue, 16 Jun 2020 09:54:01: 10000000 INFO @ Tue, 16 Jun 2020 09:54:05: 24000000 INFO @ Tue, 16 Jun 2020 09:54:06: 18000000 INFO @ Tue, 16 Jun 2020 09:54:07: 11000000 INFO @ Tue, 16 Jun 2020 09:54:10: 25000000 INFO @ Tue, 16 Jun 2020 09:54:11: 19000000 INFO @ Tue, 16 Jun 2020 09:54:13: 12000000 INFO @ Tue, 16 Jun 2020 09:54:16: 26000000 INFO @ Tue, 16 Jun 2020 09:54:17: 20000000 INFO @ Tue, 16 Jun 2020 09:54:19: 13000000 INFO @ Tue, 16 Jun 2020 09:54:21: 27000000 INFO @ Tue, 16 Jun 2020 09:54:22: 21000000 INFO @ Tue, 16 Jun 2020 09:54:25: 14000000 INFO @ Tue, 16 Jun 2020 09:54:26: 28000000 INFO @ Tue, 16 Jun 2020 09:54:27: 22000000 INFO @ Tue, 16 Jun 2020 09:54:31: 15000000 INFO @ Tue, 16 Jun 2020 09:54:32: 29000000 INFO @ Tue, 16 Jun 2020 09:54:32: 23000000 INFO @ Tue, 16 Jun 2020 09:54:37: 16000000 INFO @ Tue, 16 Jun 2020 09:54:37: 30000000 INFO @ Tue, 16 Jun 2020 09:54:38: 24000000 INFO @ Tue, 16 Jun 2020 09:54:42: 31000000 INFO @ Tue, 16 Jun 2020 09:54:42: 17000000 INFO @ Tue, 16 Jun 2020 09:54:43: 25000000 INFO @ Tue, 16 Jun 2020 09:54:48: 32000000 INFO @ Tue, 16 Jun 2020 09:54:48: 18000000 INFO @ Tue, 16 Jun 2020 09:54:49: 26000000 INFO @ Tue, 16 Jun 2020 09:54:53: 33000000 INFO @ Tue, 16 Jun 2020 09:54:54: 27000000 INFO @ Tue, 16 Jun 2020 09:54:54: 19000000 INFO @ Tue, 16 Jun 2020 09:54:58: 34000000 INFO @ Tue, 16 Jun 2020 09:54:59: 28000000 INFO @ Tue, 16 Jun 2020 09:55:00: 20000000 INFO @ Tue, 16 Jun 2020 09:55:04: 35000000 INFO @ Tue, 16 Jun 2020 09:55:05: 29000000 INFO @ Tue, 16 Jun 2020 09:55:06: 21000000 INFO @ Tue, 16 Jun 2020 09:55:09: 36000000 INFO @ Tue, 16 Jun 2020 09:55:10: 30000000 INFO @ Tue, 16 Jun 2020 09:55:12: 22000000 INFO @ Tue, 16 Jun 2020 09:55:14: 37000000 INFO @ Tue, 16 Jun 2020 09:55:15: 31000000 INFO @ Tue, 16 Jun 2020 09:55:18: 23000000 INFO @ Tue, 16 Jun 2020 09:55:20: 38000000 INFO @ Tue, 16 Jun 2020 09:55:21: 32000000 INFO @ Tue, 16 Jun 2020 09:55:24: 24000000 INFO @ Tue, 16 Jun 2020 09:55:25: 39000000 INFO @ Tue, 16 Jun 2020 09:55:27: 33000000 INFO @ Tue, 16 Jun 2020 09:55:30: 25000000 INFO @ Tue, 16 Jun 2020 09:55:31: 40000000 INFO @ Tue, 16 Jun 2020 09:55:32: 34000000 INFO @ Tue, 16 Jun 2020 09:55:36: 26000000 INFO @ Tue, 16 Jun 2020 09:55:36: 41000000 INFO @ Tue, 16 Jun 2020 09:55:38: 35000000 INFO @ Tue, 16 Jun 2020 09:55:42: 42000000 INFO @ Tue, 16 Jun 2020 09:55:42: 27000000 INFO @ Tue, 16 Jun 2020 09:55:43: 36000000 INFO @ Tue, 16 Jun 2020 09:55:47: 43000000 INFO @ Tue, 16 Jun 2020 09:55:49: 28000000 INFO @ Tue, 16 Jun 2020 09:55:49: 37000000 INFO @ Tue, 16 Jun 2020 09:55:53: 44000000 INFO @ Tue, 16 Jun 2020 09:55:54: 38000000 INFO @ Tue, 16 Jun 2020 09:55:55: 29000000 INFO @ Tue, 16 Jun 2020 09:55:59: 45000000 INFO @ Tue, 16 Jun 2020 09:56:00: 39000000 INFO @ Tue, 16 Jun 2020 09:56:01: 30000000 INFO @ Tue, 16 Jun 2020 09:56:04: 46000000 INFO @ Tue, 16 Jun 2020 09:56:06: 40000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:56:08: 31000000 INFO @ Tue, 16 Jun 2020 09:56:10: 47000000 INFO @ Tue, 16 Jun 2020 09:56:11: 41000000 INFO @ Tue, 16 Jun 2020 09:56:14: 32000000 INFO @ Tue, 16 Jun 2020 09:56:15: 48000000 INFO @ Tue, 16 Jun 2020 09:56:17: 42000000 INFO @ Tue, 16 Jun 2020 09:56:20: 33000000 INFO @ Tue, 16 Jun 2020 09:56:20: 49000000 INFO @ Tue, 16 Jun 2020 09:56:23: 43000000 INFO @ Tue, 16 Jun 2020 09:56:23: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 09:56:23: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 09:56:23: #1 total tags in treatment: 23964930 INFO @ Tue, 16 Jun 2020 09:56:23: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:56:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:56:24: #1 tags after filtering in treatment: 19096906 INFO @ Tue, 16 Jun 2020 09:56:24: #1 Redundant rate of treatment: 0.20 INFO @ Tue, 16 Jun 2020 09:56:24: #1 finished! INFO @ Tue, 16 Jun 2020 09:56:24: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:56:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:56:25: #2 number of paired peaks: 220 WARNING @ Tue, 16 Jun 2020 09:56:25: Fewer paired peaks (220) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 220 pairs to build model! INFO @ Tue, 16 Jun 2020 09:56:25: start model_add_line... INFO @ Tue, 16 Jun 2020 09:56:25: start X-correlation... INFO @ Tue, 16 Jun 2020 09:56:25: end of X-cor INFO @ Tue, 16 Jun 2020 09:56:25: #2 finished! INFO @ Tue, 16 Jun 2020 09:56:25: #2 predicted fragment length is 81 bps INFO @ Tue, 16 Jun 2020 09:56:25: #2 alternative fragment length(s) may be 4,81 bps INFO @ Tue, 16 Jun 2020 09:56:25: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4085400/SRX4085400.05_model.r WARNING @ Tue, 16 Jun 2020 09:56:25: #2 Since the d (81) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 09:56:25: #2 You may need to consider one of the other alternative d(s): 4,81 WARNING @ Tue, 16 Jun 2020 09:56:25: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 09:56:25: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:56:25: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:56:27: 34000000 INFO @ Tue, 16 Jun 2020 09:56:29: 44000000 INFO @ Tue, 16 Jun 2020 09:56:33: 35000000 INFO @ Tue, 16 Jun 2020 09:56:34: 45000000 INFO @ Tue, 16 Jun 2020 09:56:39: 36000000 INFO @ Tue, 16 Jun 2020 09:56:40: 46000000 INFO @ Tue, 16 Jun 2020 09:56:45: 37000000 INFO @ Tue, 16 Jun 2020 09:56:46: 47000000 INFO @ Tue, 16 Jun 2020 09:56:51: 48000000 INFO @ Tue, 16 Jun 2020 09:56:52: 38000000 INFO @ Tue, 16 Jun 2020 09:56:57: 49000000 INFO @ Tue, 16 Jun 2020 09:56:58: 39000000 INFO @ Tue, 16 Jun 2020 09:56:59: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 09:57:00: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 09:57:00: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 09:57:00: #1 total tags in treatment: 23964930 INFO @ Tue, 16 Jun 2020 09:57:00: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:57:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:57:00: #1 tags after filtering in treatment: 19096906 INFO @ Tue, 16 Jun 2020 09:57:00: #1 Redundant rate of treatment: 0.20 INFO @ Tue, 16 Jun 2020 09:57:00: #1 finished! INFO @ Tue, 16 Jun 2020 09:57:00: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:57:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:57:02: #2 number of paired peaks: 220 WARNING @ Tue, 16 Jun 2020 09:57:02: Fewer paired peaks (220) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 220 pairs to build model! INFO @ Tue, 16 Jun 2020 09:57:02: start model_add_line... INFO @ Tue, 16 Jun 2020 09:57:02: start X-correlation... INFO @ Tue, 16 Jun 2020 09:57:02: end of X-cor INFO @ Tue, 16 Jun 2020 09:57:02: #2 finished! INFO @ Tue, 16 Jun 2020 09:57:02: #2 predicted fragment length is 81 bps INFO @ Tue, 16 Jun 2020 09:57:02: #2 alternative fragment length(s) may be 4,81 bps INFO @ Tue, 16 Jun 2020 09:57:02: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4085400/SRX4085400.10_model.r WARNING @ Tue, 16 Jun 2020 09:57:02: #2 Since the d (81) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 09:57:02: #2 You may need to consider one of the other alternative d(s): 4,81 WARNING @ Tue, 16 Jun 2020 09:57:02: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 09:57:02: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:57:02: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:57:05: 40000000 INFO @ Tue, 16 Jun 2020 09:57:11: 41000000 INFO @ Tue, 16 Jun 2020 09:57:17: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4085400/SRX4085400.05_peaks.xls INFO @ Tue, 16 Jun 2020 09:57:17: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4085400/SRX4085400.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:57:17: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4085400/SRX4085400.05_summits.bed INFO @ Tue, 16 Jun 2020 09:57:17: Done! BigWig に変換しました。 pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (3876 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:57:17: 42000000 INFO @ Tue, 16 Jun 2020 09:57:24: 43000000 INFO @ Tue, 16 Jun 2020 09:57:30: 44000000 INFO @ Tue, 16 Jun 2020 09:57:36: 45000000 INFO @ Tue, 16 Jun 2020 09:57:37: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 09:57:42: 46000000 INFO @ Tue, 16 Jun 2020 09:57:48: 47000000 INFO @ Tue, 16 Jun 2020 09:57:54: 48000000 INFO @ Tue, 16 Jun 2020 09:57:54: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4085400/SRX4085400.10_peaks.xls INFO @ Tue, 16 Jun 2020 09:57:54: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4085400/SRX4085400.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:57:54: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4085400/SRX4085400.10_summits.bed INFO @ Tue, 16 Jun 2020 09:57:54: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (2188 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:58:00: 49000000 INFO @ Tue, 16 Jun 2020 09:58:03: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 09:58:03: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 09:58:03: #1 total tags in treatment: 23964930 INFO @ Tue, 16 Jun 2020 09:58:03: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:58:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:58:04: #1 tags after filtering in treatment: 19096906 INFO @ Tue, 16 Jun 2020 09:58:04: #1 Redundant rate of treatment: 0.20 INFO @ Tue, 16 Jun 2020 09:58:04: #1 finished! INFO @ Tue, 16 Jun 2020 09:58:04: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:58:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:58:05: #2 number of paired peaks: 220 WARNING @ Tue, 16 Jun 2020 09:58:05: Fewer paired peaks (220) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 220 pairs to build model! INFO @ Tue, 16 Jun 2020 09:58:05: start model_add_line... INFO @ Tue, 16 Jun 2020 09:58:05: start X-correlation... INFO @ Tue, 16 Jun 2020 09:58:05: end of X-cor INFO @ Tue, 16 Jun 2020 09:58:05: #2 finished! INFO @ Tue, 16 Jun 2020 09:58:05: #2 predicted fragment length is 81 bps INFO @ Tue, 16 Jun 2020 09:58:05: #2 alternative fragment length(s) may be 4,81 bps INFO @ Tue, 16 Jun 2020 09:58:05: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4085400/SRX4085400.20_model.r WARNING @ Tue, 16 Jun 2020 09:58:05: #2 Since the d (81) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 09:58:05: #2 You may need to consider one of the other alternative d(s): 4,81 WARNING @ Tue, 16 Jun 2020 09:58:05: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 09:58:05: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:58:05: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:58:40: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 09:58:58: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4085400/SRX4085400.20_peaks.xls INFO @ Tue, 16 Jun 2020 09:58:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4085400/SRX4085400.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:58:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4085400/SRX4085400.20_summits.bed INFO @ Tue, 16 Jun 2020 09:58:58: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (970 records, 4 fields): 3 millis CompletedMACS2peakCalling