Job ID = 6507774 SRX = SRX4085365 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-06-26T13:08:52 prefetch.2.10.7: 1) Downloading 'SRR7167394'... 2020-06-26T13:08:52 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T13:15:58 prefetch.2.10.7: HTTPS download succeed 2020-06-26T13:15:58 prefetch.2.10.7: 1) 'SRR7167394' was downloaded successfully Read 38347298 spots for SRR7167394/SRR7167394.sra Written 38347298 spots for SRR7167394/SRR7167394.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:28:27 38347298 reads; of these: 38347298 (100.00%) were paired; of these: 5832823 (15.21%) aligned concordantly 0 times 27829323 (72.57%) aligned concordantly exactly 1 time 4685152 (12.22%) aligned concordantly >1 times ---- 5832823 pairs aligned concordantly 0 times; of these: 293904 (5.04%) aligned discordantly 1 time ---- 5538919 pairs aligned 0 times concordantly or discordantly; of these: 11077838 mates make up the pairs; of these: 9153957 (82.63%) aligned 0 times 1584394 (14.30%) aligned exactly 1 time 339487 (3.06%) aligned >1 times 88.06% overall alignment rate Time searching: 00:28:27 Overall time: 00:28:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 2414356 / 32693366 = 0.0738 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 23:07:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4085365/SRX4085365.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4085365/SRX4085365.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4085365/SRX4085365.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4085365/SRX4085365.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 23:07:17: #1 read tag files... INFO @ Fri, 26 Jun 2020 23:07:17: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 23:07:22: 1000000 INFO @ Fri, 26 Jun 2020 23:07:27: 2000000 INFO @ Fri, 26 Jun 2020 23:07:33: 3000000 INFO @ Fri, 26 Jun 2020 23:07:38: 4000000 INFO @ Fri, 26 Jun 2020 23:07:43: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 23:07:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4085365/SRX4085365.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4085365/SRX4085365.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4085365/SRX4085365.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4085365/SRX4085365.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 23:07:47: #1 read tag files... INFO @ Fri, 26 Jun 2020 23:07:47: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 23:07:49: 6000000 INFO @ Fri, 26 Jun 2020 23:07:54: 1000000 INFO @ Fri, 26 Jun 2020 23:07:55: 7000000 INFO @ Fri, 26 Jun 2020 23:08:00: 2000000 INFO @ Fri, 26 Jun 2020 23:08:01: 8000000 INFO @ Fri, 26 Jun 2020 23:08:07: 3000000 INFO @ Fri, 26 Jun 2020 23:08:07: 9000000 INFO @ Fri, 26 Jun 2020 23:08:14: 10000000 INFO @ Fri, 26 Jun 2020 23:08:14: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 23:08:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4085365/SRX4085365.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4085365/SRX4085365.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4085365/SRX4085365.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4085365/SRX4085365.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 23:08:17: #1 read tag files... INFO @ Fri, 26 Jun 2020 23:08:17: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 23:08:20: 11000000 INFO @ Fri, 26 Jun 2020 23:08:21: 5000000 INFO @ Fri, 26 Jun 2020 23:08:23: 1000000 INFO @ Fri, 26 Jun 2020 23:08:26: 12000000 INFO @ Fri, 26 Jun 2020 23:08:28: 6000000 INFO @ Fri, 26 Jun 2020 23:08:30: 2000000 INFO @ Fri, 26 Jun 2020 23:08:33: 13000000 INFO @ Fri, 26 Jun 2020 23:08:34: 7000000 INFO @ Fri, 26 Jun 2020 23:08:36: 3000000 INFO @ Fri, 26 Jun 2020 23:08:39: 14000000 INFO @ Fri, 26 Jun 2020 23:08:41: 8000000 INFO @ Fri, 26 Jun 2020 23:08:43: 4000000 INFO @ Fri, 26 Jun 2020 23:08:45: 15000000 INFO @ Fri, 26 Jun 2020 23:08:49: 9000000 INFO @ Fri, 26 Jun 2020 23:08:49: 5000000 INFO @ Fri, 26 Jun 2020 23:08:52: 16000000 INFO @ Fri, 26 Jun 2020 23:08:55: 6000000 INFO @ Fri, 26 Jun 2020 23:08:56: 10000000 INFO @ Fri, 26 Jun 2020 23:08:58: 17000000 INFO @ Fri, 26 Jun 2020 23:09:02: 7000000 INFO @ Fri, 26 Jun 2020 23:09:03: 11000000 INFO @ Fri, 26 Jun 2020 23:09:05: 18000000 INFO @ Fri, 26 Jun 2020 23:09:08: 8000000 INFO @ Fri, 26 Jun 2020 23:09:10: 12000000 INFO @ Fri, 26 Jun 2020 23:09:11: 19000000 INFO @ Fri, 26 Jun 2020 23:09:15: 9000000 INFO @ Fri, 26 Jun 2020 23:09:17: 13000000 INFO @ Fri, 26 Jun 2020 23:09:18: 20000000 INFO @ Fri, 26 Jun 2020 23:09:21: 10000000 INFO @ Fri, 26 Jun 2020 23:09:24: 14000000 INFO @ Fri, 26 Jun 2020 23:09:24: 21000000 INFO @ Fri, 26 Jun 2020 23:09:27: 11000000 INFO @ Fri, 26 Jun 2020 23:09:30: 22000000 INFO @ Fri, 26 Jun 2020 23:09:30: 15000000 INFO @ Fri, 26 Jun 2020 23:09:34: 12000000 INFO @ Fri, 26 Jun 2020 23:09:36: 23000000 INFO @ Fri, 26 Jun 2020 23:09:37: 16000000 INFO @ Fri, 26 Jun 2020 23:09:40: 13000000 INFO @ Fri, 26 Jun 2020 23:09:43: 24000000 INFO @ Fri, 26 Jun 2020 23:09:44: 17000000 INFO @ Fri, 26 Jun 2020 23:09:47: 14000000 INFO @ Fri, 26 Jun 2020 23:09:49: 25000000 INFO @ Fri, 26 Jun 2020 23:09:51: 18000000 INFO @ Fri, 26 Jun 2020 23:09:53: 15000000 INFO @ Fri, 26 Jun 2020 23:09:55: 26000000 INFO @ Fri, 26 Jun 2020 23:09:58: 19000000 INFO @ Fri, 26 Jun 2020 23:10:00: 16000000 INFO @ Fri, 26 Jun 2020 23:10:02: 27000000 INFO @ Fri, 26 Jun 2020 23:10:05: 20000000 INFO @ Fri, 26 Jun 2020 23:10:06: 17000000 INFO @ Fri, 26 Jun 2020 23:10:08: 28000000 INFO @ Fri, 26 Jun 2020 23:10:12: 21000000 INFO @ Fri, 26 Jun 2020 23:10:12: 18000000 INFO @ Fri, 26 Jun 2020 23:10:14: 29000000 INFO @ Fri, 26 Jun 2020 23:10:18: 22000000 INFO @ Fri, 26 Jun 2020 23:10:19: 19000000 INFO @ Fri, 26 Jun 2020 23:10:20: 30000000 INFO @ Fri, 26 Jun 2020 23:10:25: 20000000 INFO @ Fri, 26 Jun 2020 23:10:25: 23000000 INFO @ Fri, 26 Jun 2020 23:10:26: 31000000 INFO @ Fri, 26 Jun 2020 23:10:31: 21000000 INFO @ Fri, 26 Jun 2020 23:10:32: 24000000 INFO @ Fri, 26 Jun 2020 23:10:32: 32000000 INFO @ Fri, 26 Jun 2020 23:10:37: 22000000 INFO @ Fri, 26 Jun 2020 23:10:39: 33000000 INFO @ Fri, 26 Jun 2020 23:10:39: 25000000 INFO @ Fri, 26 Jun 2020 23:10:43: 23000000 INFO @ Fri, 26 Jun 2020 23:10:45: 34000000 INFO @ Fri, 26 Jun 2020 23:10:46: 26000000 INFO @ Fri, 26 Jun 2020 23:10:49: 24000000 INFO @ Fri, 26 Jun 2020 23:10:51: 35000000 INFO @ Fri, 26 Jun 2020 23:10:53: 27000000 INFO @ Fri, 26 Jun 2020 23:10:55: 25000000 INFO @ Fri, 26 Jun 2020 23:10:57: 36000000 INFO @ Fri, 26 Jun 2020 23:10:59: 28000000 INFO @ Fri, 26 Jun 2020 23:11:02: 26000000 INFO @ Fri, 26 Jun 2020 23:11:03: 37000000 INFO @ Fri, 26 Jun 2020 23:11:06: 29000000 INFO @ Fri, 26 Jun 2020 23:11:08: 27000000 INFO @ Fri, 26 Jun 2020 23:11:09: 38000000 INFO @ Fri, 26 Jun 2020 23:11:13: 30000000 INFO @ Fri, 26 Jun 2020 23:11:14: 28000000 INFO @ Fri, 26 Jun 2020 23:11:15: 39000000 INFO @ Fri, 26 Jun 2020 23:11:19: 31000000 INFO @ Fri, 26 Jun 2020 23:11:20: 29000000 INFO @ Fri, 26 Jun 2020 23:11:21: 40000000 INFO @ Fri, 26 Jun 2020 23:11:26: 30000000 INFO @ Fri, 26 Jun 2020 23:11:26: 32000000 INFO @ Fri, 26 Jun 2020 23:11:27: 41000000 INFO @ Fri, 26 Jun 2020 23:11:32: 31000000 INFO @ Fri, 26 Jun 2020 23:11:33: 33000000 INFO @ Fri, 26 Jun 2020 23:11:33: 42000000 INFO @ Fri, 26 Jun 2020 23:11:38: 32000000 INFO @ Fri, 26 Jun 2020 23:11:39: 34000000 INFO @ Fri, 26 Jun 2020 23:11:39: 43000000 INFO @ Fri, 26 Jun 2020 23:11:44: 33000000 INFO @ Fri, 26 Jun 2020 23:11:45: 44000000 INFO @ Fri, 26 Jun 2020 23:11:46: 35000000 INFO @ Fri, 26 Jun 2020 23:11:50: 34000000 INFO @ Fri, 26 Jun 2020 23:11:52: 45000000 INFO @ Fri, 26 Jun 2020 23:11:53: 36000000 INFO @ Fri, 26 Jun 2020 23:11:57: 35000000 INFO @ Fri, 26 Jun 2020 23:11:58: 46000000 INFO @ Fri, 26 Jun 2020 23:11:59: 37000000 INFO @ Fri, 26 Jun 2020 23:12:03: 36000000 INFO @ Fri, 26 Jun 2020 23:12:04: 47000000 INFO @ Fri, 26 Jun 2020 23:12:06: 38000000 INFO @ Fri, 26 Jun 2020 23:12:09: 37000000 INFO @ Fri, 26 Jun 2020 23:12:10: 48000000 INFO @ Fri, 26 Jun 2020 23:12:12: 39000000 INFO @ Fri, 26 Jun 2020 23:12:15: 38000000 INFO @ Fri, 26 Jun 2020 23:12:16: 49000000 INFO @ Fri, 26 Jun 2020 23:12:19: 40000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 23:12:21: 39000000 INFO @ Fri, 26 Jun 2020 23:12:22: 50000000 INFO @ Fri, 26 Jun 2020 23:12:25: 41000000 INFO @ Fri, 26 Jun 2020 23:12:27: 40000000 INFO @ Fri, 26 Jun 2020 23:12:28: 51000000 INFO @ Fri, 26 Jun 2020 23:12:32: 42000000 INFO @ Fri, 26 Jun 2020 23:12:33: 41000000 INFO @ Fri, 26 Jun 2020 23:12:34: 52000000 INFO @ Fri, 26 Jun 2020 23:12:38: 43000000 INFO @ Fri, 26 Jun 2020 23:12:39: 42000000 INFO @ Fri, 26 Jun 2020 23:12:40: 53000000 INFO @ Fri, 26 Jun 2020 23:12:45: 44000000 INFO @ Fri, 26 Jun 2020 23:12:45: 43000000 INFO @ Fri, 26 Jun 2020 23:12:46: 54000000 INFO @ Fri, 26 Jun 2020 23:12:51: 44000000 INFO @ Fri, 26 Jun 2020 23:12:52: 45000000 INFO @ Fri, 26 Jun 2020 23:12:52: 55000000 INFO @ Fri, 26 Jun 2020 23:12:58: 45000000 INFO @ Fri, 26 Jun 2020 23:12:58: 56000000 INFO @ Fri, 26 Jun 2020 23:12:58: 46000000 INFO @ Fri, 26 Jun 2020 23:13:04: 46000000 INFO @ Fri, 26 Jun 2020 23:13:04: 57000000 INFO @ Fri, 26 Jun 2020 23:13:05: 47000000 INFO @ Fri, 26 Jun 2020 23:13:10: 47000000 INFO @ Fri, 26 Jun 2020 23:13:10: 58000000 INFO @ Fri, 26 Jun 2020 23:13:11: 48000000 INFO @ Fri, 26 Jun 2020 23:13:16: 48000000 INFO @ Fri, 26 Jun 2020 23:13:17: 59000000 INFO @ Fri, 26 Jun 2020 23:13:18: 49000000 INFO @ Fri, 26 Jun 2020 23:13:22: 49000000 INFO @ Fri, 26 Jun 2020 23:13:23: 60000000 INFO @ Fri, 26 Jun 2020 23:13:25: 50000000 INFO @ Fri, 26 Jun 2020 23:13:28: 50000000 INFO @ Fri, 26 Jun 2020 23:13:29: 61000000 INFO @ Fri, 26 Jun 2020 23:13:31: 51000000 INFO @ Fri, 26 Jun 2020 23:13:35: 51000000 INFO @ Fri, 26 Jun 2020 23:13:35: 62000000 INFO @ Fri, 26 Jun 2020 23:13:38: 52000000 INFO @ Fri, 26 Jun 2020 23:13:40: #1 tag size is determined as 51 bps INFO @ Fri, 26 Jun 2020 23:13:40: #1 tag size = 51 INFO @ Fri, 26 Jun 2020 23:13:40: #1 total tags in treatment: 30111392 INFO @ Fri, 26 Jun 2020 23:13:40: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 23:13:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 23:13:40: #1 tags after filtering in treatment: 26257538 INFO @ Fri, 26 Jun 2020 23:13:40: #1 Redundant rate of treatment: 0.13 INFO @ Fri, 26 Jun 2020 23:13:40: #1 finished! INFO @ Fri, 26 Jun 2020 23:13:40: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 23:13:40: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 23:13:41: 52000000 BigWig に変換しました。 INFO @ Fri, 26 Jun 2020 23:13:42: #2 number of paired peaks: 120 WARNING @ Fri, 26 Jun 2020 23:13:42: Fewer paired peaks (120) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 120 pairs to build model! INFO @ Fri, 26 Jun 2020 23:13:42: start model_add_line... INFO @ Fri, 26 Jun 2020 23:13:42: start X-correlation... INFO @ Fri, 26 Jun 2020 23:13:42: end of X-cor INFO @ Fri, 26 Jun 2020 23:13:42: #2 finished! INFO @ Fri, 26 Jun 2020 23:13:42: #2 predicted fragment length is 59 bps INFO @ Fri, 26 Jun 2020 23:13:42: #2 alternative fragment length(s) may be 2,59,598 bps INFO @ Fri, 26 Jun 2020 23:13:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4085365/SRX4085365.05_model.r WARNING @ Fri, 26 Jun 2020 23:13:42: #2 Since the d (59) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 26 Jun 2020 23:13:42: #2 You may need to consider one of the other alternative d(s): 2,59,598 WARNING @ Fri, 26 Jun 2020 23:13:42: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 26 Jun 2020 23:13:42: #3 Call peaks... INFO @ Fri, 26 Jun 2020 23:13:42: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 26 Jun 2020 23:13:45: 53000000 INFO @ Fri, 26 Jun 2020 23:13:47: 53000000 INFO @ Fri, 26 Jun 2020 23:13:53: 54000000 INFO @ Fri, 26 Jun 2020 23:13:53: 54000000 INFO @ Fri, 26 Jun 2020 23:14:00: 55000000 INFO @ Fri, 26 Jun 2020 23:14:00: 55000000 INFO @ Fri, 26 Jun 2020 23:14:08: 56000000 INFO @ Fri, 26 Jun 2020 23:14:08: 56000000 INFO @ Fri, 26 Jun 2020 23:14:16: 57000000 INFO @ Fri, 26 Jun 2020 23:14:16: 57000000 INFO @ Fri, 26 Jun 2020 23:14:22: #3 Call peaks for each chromosome... INFO @ Fri, 26 Jun 2020 23:14:24: 58000000 INFO @ Fri, 26 Jun 2020 23:14:24: 58000000 INFO @ Fri, 26 Jun 2020 23:14:32: 59000000 INFO @ Fri, 26 Jun 2020 23:14:32: 59000000 INFO @ Fri, 26 Jun 2020 23:14:40: 60000000 INFO @ Fri, 26 Jun 2020 23:14:40: 60000000 INFO @ Fri, 26 Jun 2020 23:14:41: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4085365/SRX4085365.05_peaks.xls INFO @ Fri, 26 Jun 2020 23:14:41: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4085365/SRX4085365.05_peaks.narrowPeak INFO @ Fri, 26 Jun 2020 23:14:41: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4085365/SRX4085365.05_summits.bed INFO @ Fri, 26 Jun 2020 23:14:41: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (748 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 23:14:49: 61000000 INFO @ Fri, 26 Jun 2020 23:14:49: 61000000 INFO @ Fri, 26 Jun 2020 23:14:57: 62000000 INFO @ Fri, 26 Jun 2020 23:14:57: 62000000 INFO @ Fri, 26 Jun 2020 23:15:02: #1 tag size is determined as 51 bps INFO @ Fri, 26 Jun 2020 23:15:02: #1 tag size = 51 INFO @ Fri, 26 Jun 2020 23:15:02: #1 total tags in treatment: 30111392 INFO @ Fri, 26 Jun 2020 23:15:02: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 23:15:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 23:15:02: #1 tag size is determined as 51 bps INFO @ Fri, 26 Jun 2020 23:15:02: #1 tag size = 51 INFO @ Fri, 26 Jun 2020 23:15:02: #1 total tags in treatment: 30111392 INFO @ Fri, 26 Jun 2020 23:15:02: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 23:15:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 23:15:03: #1 tags after filtering in treatment: 26257538 INFO @ Fri, 26 Jun 2020 23:15:03: #1 Redundant rate of treatment: 0.13 INFO @ Fri, 26 Jun 2020 23:15:03: #1 finished! INFO @ Fri, 26 Jun 2020 23:15:03: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 23:15:03: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 23:15:03: #1 tags after filtering in treatment: 26257538 INFO @ Fri, 26 Jun 2020 23:15:03: #1 Redundant rate of treatment: 0.13 INFO @ Fri, 26 Jun 2020 23:15:03: #1 finished! INFO @ Fri, 26 Jun 2020 23:15:03: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 23:15:03: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 23:15:04: #2 number of paired peaks: 120 WARNING @ Fri, 26 Jun 2020 23:15:04: Fewer paired peaks (120) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 120 pairs to build model! INFO @ Fri, 26 Jun 2020 23:15:04: start model_add_line... INFO @ Fri, 26 Jun 2020 23:15:04: #2 number of paired peaks: 120 WARNING @ Fri, 26 Jun 2020 23:15:04: Fewer paired peaks (120) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 120 pairs to build model! INFO @ Fri, 26 Jun 2020 23:15:04: start model_add_line... INFO @ Fri, 26 Jun 2020 23:15:04: start X-correlation... INFO @ Fri, 26 Jun 2020 23:15:04: end of X-cor INFO @ Fri, 26 Jun 2020 23:15:04: #2 finished! INFO @ Fri, 26 Jun 2020 23:15:04: #2 predicted fragment length is 59 bps INFO @ Fri, 26 Jun 2020 23:15:04: #2 alternative fragment length(s) may be 2,59,598 bps INFO @ Fri, 26 Jun 2020 23:15:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4085365/SRX4085365.20_model.r INFO @ Fri, 26 Jun 2020 23:15:04: start X-correlation... WARNING @ Fri, 26 Jun 2020 23:15:04: #2 Since the d (59) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 26 Jun 2020 23:15:04: #2 You may need to consider one of the other alternative d(s): 2,59,598 WARNING @ Fri, 26 Jun 2020 23:15:04: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 26 Jun 2020 23:15:04: #3 Call peaks... INFO @ Fri, 26 Jun 2020 23:15:04: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 26 Jun 2020 23:15:04: end of X-cor INFO @ Fri, 26 Jun 2020 23:15:04: #2 finished! INFO @ Fri, 26 Jun 2020 23:15:04: #2 predicted fragment length is 59 bps INFO @ Fri, 26 Jun 2020 23:15:04: #2 alternative fragment length(s) may be 2,59,598 bps INFO @ Fri, 26 Jun 2020 23:15:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4085365/SRX4085365.10_model.r WARNING @ Fri, 26 Jun 2020 23:15:04: #2 Since the d (59) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 26 Jun 2020 23:15:04: #2 You may need to consider one of the other alternative d(s): 2,59,598 WARNING @ Fri, 26 Jun 2020 23:15:04: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 26 Jun 2020 23:15:04: #3 Call peaks... INFO @ Fri, 26 Jun 2020 23:15:04: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 26 Jun 2020 23:15:42: #3 Call peaks for each chromosome... INFO @ Fri, 26 Jun 2020 23:15:45: #3 Call peaks for each chromosome... INFO @ Fri, 26 Jun 2020 23:16:00: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4085365/SRX4085365.10_peaks.xls INFO @ Fri, 26 Jun 2020 23:16:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4085365/SRX4085365.10_peaks.narrowPeak INFO @ Fri, 26 Jun 2020 23:16:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4085365/SRX4085365.10_summits.bed INFO @ Fri, 26 Jun 2020 23:16:00: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (377 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 23:16:03: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4085365/SRX4085365.20_peaks.xls INFO @ Fri, 26 Jun 2020 23:16:03: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4085365/SRX4085365.20_peaks.narrowPeak INFO @ Fri, 26 Jun 2020 23:16:03: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4085365/SRX4085365.20_summits.bed INFO @ Fri, 26 Jun 2020 23:16:03: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (127 records, 4 fields): 1 millis CompletedMACS2peakCalling