Job ID = 6367791 SRX = SRX4085359 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-06-15T23:31:35 prefetch.2.10.7: 1) Downloading 'SRR7167388'... 2020-06-15T23:31:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:47:58 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:47:58 prefetch.2.10.7: 1) 'SRR7167388' was downloaded successfully Read 31585738 spots for SRR7167388/SRR7167388.sra Written 31585738 spots for SRR7167388/SRR7167388.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:27:23 31585738 reads; of these: 31585738 (100.00%) were paired; of these: 1259156 (3.99%) aligned concordantly 0 times 26290887 (83.24%) aligned concordantly exactly 1 time 4035695 (12.78%) aligned concordantly >1 times ---- 1259156 pairs aligned concordantly 0 times; of these: 222846 (17.70%) aligned discordantly 1 time ---- 1036310 pairs aligned 0 times concordantly or discordantly; of these: 2072620 mates make up the pairs; of these: 1237806 (59.72%) aligned 0 times 639767 (30.87%) aligned exactly 1 time 195047 (9.41%) aligned >1 times 98.04% overall alignment rate Time searching: 00:27:23 Overall time: 00:27:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 5067638 / 30455256 = 0.1664 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:34:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4085359/SRX4085359.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4085359/SRX4085359.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4085359/SRX4085359.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4085359/SRX4085359.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:34:42: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:34:42: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:34:47: 1000000 INFO @ Tue, 16 Jun 2020 09:34:51: 2000000 INFO @ Tue, 16 Jun 2020 09:34:56: 3000000 INFO @ Tue, 16 Jun 2020 09:35:01: 4000000 INFO @ Tue, 16 Jun 2020 09:35:06: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:35:11: 6000000 INFO @ Tue, 16 Jun 2020 09:35:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4085359/SRX4085359.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4085359/SRX4085359.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4085359/SRX4085359.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4085359/SRX4085359.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:35:12: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:35:12: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:35:15: 7000000 INFO @ Tue, 16 Jun 2020 09:35:17: 1000000 INFO @ Tue, 16 Jun 2020 09:35:20: 8000000 INFO @ Tue, 16 Jun 2020 09:35:22: 2000000 INFO @ Tue, 16 Jun 2020 09:35:25: 9000000 INFO @ Tue, 16 Jun 2020 09:35:27: 3000000 INFO @ Tue, 16 Jun 2020 09:35:30: 10000000 INFO @ Tue, 16 Jun 2020 09:35:32: 4000000 INFO @ Tue, 16 Jun 2020 09:35:35: 11000000 INFO @ Tue, 16 Jun 2020 09:35:37: 5000000 INFO @ Tue, 16 Jun 2020 09:35:40: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:35:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4085359/SRX4085359.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4085359/SRX4085359.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4085359/SRX4085359.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4085359/SRX4085359.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:35:42: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:35:42: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:35:42: 6000000 INFO @ Tue, 16 Jun 2020 09:35:44: 13000000 INFO @ Tue, 16 Jun 2020 09:35:47: 7000000 INFO @ Tue, 16 Jun 2020 09:35:47: 1000000 INFO @ Tue, 16 Jun 2020 09:35:49: 14000000 INFO @ Tue, 16 Jun 2020 09:35:52: 8000000 INFO @ Tue, 16 Jun 2020 09:35:52: 2000000 INFO @ Tue, 16 Jun 2020 09:35:54: 15000000 INFO @ Tue, 16 Jun 2020 09:35:56: 9000000 INFO @ Tue, 16 Jun 2020 09:35:57: 3000000 INFO @ Tue, 16 Jun 2020 09:35:59: 16000000 INFO @ Tue, 16 Jun 2020 09:36:01: 10000000 INFO @ Tue, 16 Jun 2020 09:36:02: 4000000 INFO @ Tue, 16 Jun 2020 09:36:04: 17000000 INFO @ Tue, 16 Jun 2020 09:36:06: 11000000 INFO @ Tue, 16 Jun 2020 09:36:07: 5000000 INFO @ Tue, 16 Jun 2020 09:36:09: 18000000 INFO @ Tue, 16 Jun 2020 09:36:11: 12000000 INFO @ Tue, 16 Jun 2020 09:36:12: 6000000 INFO @ Tue, 16 Jun 2020 09:36:14: 19000000 INFO @ Tue, 16 Jun 2020 09:36:16: 13000000 INFO @ Tue, 16 Jun 2020 09:36:16: 7000000 INFO @ Tue, 16 Jun 2020 09:36:19: 20000000 INFO @ Tue, 16 Jun 2020 09:36:20: 14000000 INFO @ Tue, 16 Jun 2020 09:36:21: 8000000 INFO @ Tue, 16 Jun 2020 09:36:23: 21000000 INFO @ Tue, 16 Jun 2020 09:36:25: 15000000 INFO @ Tue, 16 Jun 2020 09:36:26: 9000000 INFO @ Tue, 16 Jun 2020 09:36:28: 22000000 INFO @ Tue, 16 Jun 2020 09:36:30: 16000000 INFO @ Tue, 16 Jun 2020 09:36:31: 10000000 INFO @ Tue, 16 Jun 2020 09:36:33: 23000000 INFO @ Tue, 16 Jun 2020 09:36:35: 17000000 INFO @ Tue, 16 Jun 2020 09:36:36: 11000000 INFO @ Tue, 16 Jun 2020 09:36:38: 24000000 INFO @ Tue, 16 Jun 2020 09:36:40: 18000000 INFO @ Tue, 16 Jun 2020 09:36:41: 12000000 INFO @ Tue, 16 Jun 2020 09:36:42: 25000000 INFO @ Tue, 16 Jun 2020 09:36:44: 19000000 INFO @ Tue, 16 Jun 2020 09:36:45: 13000000 INFO @ Tue, 16 Jun 2020 09:36:47: 26000000 INFO @ Tue, 16 Jun 2020 09:36:49: 20000000 INFO @ Tue, 16 Jun 2020 09:36:50: 14000000 INFO @ Tue, 16 Jun 2020 09:36:52: 27000000 INFO @ Tue, 16 Jun 2020 09:36:54: 21000000 INFO @ Tue, 16 Jun 2020 09:36:55: 15000000 INFO @ Tue, 16 Jun 2020 09:36:56: 28000000 INFO @ Tue, 16 Jun 2020 09:36:58: 22000000 INFO @ Tue, 16 Jun 2020 09:37:00: 16000000 INFO @ Tue, 16 Jun 2020 09:37:01: 29000000 INFO @ Tue, 16 Jun 2020 09:37:03: 23000000 INFO @ Tue, 16 Jun 2020 09:37:04: 17000000 INFO @ Tue, 16 Jun 2020 09:37:06: 30000000 INFO @ Tue, 16 Jun 2020 09:37:08: 24000000 INFO @ Tue, 16 Jun 2020 09:37:09: 18000000 INFO @ Tue, 16 Jun 2020 09:37:10: 31000000 INFO @ Tue, 16 Jun 2020 09:37:13: 25000000 INFO @ Tue, 16 Jun 2020 09:37:14: 19000000 INFO @ Tue, 16 Jun 2020 09:37:15: 32000000 INFO @ Tue, 16 Jun 2020 09:37:17: 26000000 INFO @ Tue, 16 Jun 2020 09:37:19: 20000000 INFO @ Tue, 16 Jun 2020 09:37:20: 33000000 INFO @ Tue, 16 Jun 2020 09:37:22: 27000000 INFO @ Tue, 16 Jun 2020 09:37:23: 21000000 INFO @ Tue, 16 Jun 2020 09:37:24: 34000000 INFO @ Tue, 16 Jun 2020 09:37:27: 28000000 INFO @ Tue, 16 Jun 2020 09:37:28: 22000000 INFO @ Tue, 16 Jun 2020 09:37:29: 35000000 INFO @ Tue, 16 Jun 2020 09:37:31: 29000000 INFO @ Tue, 16 Jun 2020 09:37:33: 23000000 INFO @ Tue, 16 Jun 2020 09:37:34: 36000000 INFO @ Tue, 16 Jun 2020 09:37:36: 30000000 INFO @ Tue, 16 Jun 2020 09:37:38: 24000000 INFO @ Tue, 16 Jun 2020 09:37:38: 37000000 INFO @ Tue, 16 Jun 2020 09:37:40: 31000000 INFO @ Tue, 16 Jun 2020 09:37:42: 25000000 INFO @ Tue, 16 Jun 2020 09:37:43: 38000000 INFO @ Tue, 16 Jun 2020 09:37:45: 32000000 INFO @ Tue, 16 Jun 2020 09:37:47: 26000000 INFO @ Tue, 16 Jun 2020 09:37:48: 39000000 INFO @ Tue, 16 Jun 2020 09:37:50: 33000000 INFO @ Tue, 16 Jun 2020 09:37:52: 27000000 INFO @ Tue, 16 Jun 2020 09:37:52: 40000000 INFO @ Tue, 16 Jun 2020 09:37:54: 34000000 INFO @ Tue, 16 Jun 2020 09:37:56: 28000000 INFO @ Tue, 16 Jun 2020 09:37:57: 41000000 INFO @ Tue, 16 Jun 2020 09:37:59: 35000000 INFO @ Tue, 16 Jun 2020 09:38:01: 29000000 INFO @ Tue, 16 Jun 2020 09:38:02: 42000000 INFO @ Tue, 16 Jun 2020 09:38:04: 36000000 INFO @ Tue, 16 Jun 2020 09:38:05: 30000000 INFO @ Tue, 16 Jun 2020 09:38:06: 43000000 INFO @ Tue, 16 Jun 2020 09:38:09: 37000000 INFO @ Tue, 16 Jun 2020 09:38:10: 31000000 INFO @ Tue, 16 Jun 2020 09:38:11: 44000000 INFO @ Tue, 16 Jun 2020 09:38:14: 38000000 INFO @ Tue, 16 Jun 2020 09:38:14: 32000000 INFO @ Tue, 16 Jun 2020 09:38:15: 45000000 INFO @ Tue, 16 Jun 2020 09:38:19: 39000000 INFO @ Tue, 16 Jun 2020 09:38:19: 33000000 INFO @ Tue, 16 Jun 2020 09:38:20: 46000000 INFO @ Tue, 16 Jun 2020 09:38:24: 40000000 INFO @ Tue, 16 Jun 2020 09:38:24: 34000000 INFO @ Tue, 16 Jun 2020 09:38:24: 47000000 INFO @ Tue, 16 Jun 2020 09:38:29: 35000000 INFO @ Tue, 16 Jun 2020 09:38:29: 41000000 INFO @ Tue, 16 Jun 2020 09:38:29: 48000000 INFO @ Tue, 16 Jun 2020 09:38:34: 36000000 INFO @ Tue, 16 Jun 2020 09:38:34: 49000000 INFO @ Tue, 16 Jun 2020 09:38:34: 42000000 INFO @ Tue, 16 Jun 2020 09:38:38: 50000000 INFO @ Tue, 16 Jun 2020 09:38:38: 37000000 INFO @ Tue, 16 Jun 2020 09:38:39: 43000000 INFO @ Tue, 16 Jun 2020 09:38:43: 51000000 INFO @ Tue, 16 Jun 2020 09:38:43: 38000000 INFO @ Tue, 16 Jun 2020 09:38:43: 44000000 INFO @ Tue, 16 Jun 2020 09:38:47: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 09:38:47: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 09:38:47: #1 total tags in treatment: 25273748 INFO @ Tue, 16 Jun 2020 09:38:47: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:38:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:38:47: #1 tags after filtering in treatment: 20085425 INFO @ Tue, 16 Jun 2020 09:38:47: #1 Redundant rate of treatment: 0.21 INFO @ Tue, 16 Jun 2020 09:38:47: #1 finished! INFO @ Tue, 16 Jun 2020 09:38:47: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:38:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:38:48: 39000000 INFO @ Tue, 16 Jun 2020 09:38:48: 45000000 INFO @ Tue, 16 Jun 2020 09:38:49: #2 number of paired peaks: 141 WARNING @ Tue, 16 Jun 2020 09:38:49: Fewer paired peaks (141) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 141 pairs to build model! INFO @ Tue, 16 Jun 2020 09:38:49: start model_add_line... INFO @ Tue, 16 Jun 2020 09:38:49: start X-correlation... INFO @ Tue, 16 Jun 2020 09:38:49: end of X-cor INFO @ Tue, 16 Jun 2020 09:38:49: #2 finished! INFO @ Tue, 16 Jun 2020 09:38:49: #2 predicted fragment length is 56 bps INFO @ Tue, 16 Jun 2020 09:38:49: #2 alternative fragment length(s) may be 1,56,95,112,136 bps INFO @ Tue, 16 Jun 2020 09:38:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4085359/SRX4085359.05_model.r WARNING @ Tue, 16 Jun 2020 09:38:49: #2 Since the d (56) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 09:38:49: #2 You may need to consider one of the other alternative d(s): 1,56,95,112,136 WARNING @ Tue, 16 Jun 2020 09:38:49: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 09:38:49: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:38:49: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:38:53: 46000000 INFO @ Tue, 16 Jun 2020 09:38:53: 40000000 INFO @ Tue, 16 Jun 2020 09:38:57: 47000000 INFO @ Tue, 16 Jun 2020 09:38:58: 41000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:39:02: 48000000 INFO @ Tue, 16 Jun 2020 09:39:02: 42000000 INFO @ Tue, 16 Jun 2020 09:39:07: 49000000 INFO @ Tue, 16 Jun 2020 09:39:07: 43000000 INFO @ Tue, 16 Jun 2020 09:39:11: 50000000 INFO @ Tue, 16 Jun 2020 09:39:11: 44000000 INFO @ Tue, 16 Jun 2020 09:39:16: 51000000 INFO @ Tue, 16 Jun 2020 09:39:16: 45000000 INFO @ Tue, 16 Jun 2020 09:39:20: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 09:39:20: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 09:39:20: #1 total tags in treatment: 25273748 INFO @ Tue, 16 Jun 2020 09:39:20: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:39:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:39:20: #1 tags after filtering in treatment: 20085425 INFO @ Tue, 16 Jun 2020 09:39:20: #1 Redundant rate of treatment: 0.21 INFO @ Tue, 16 Jun 2020 09:39:20: #1 finished! INFO @ Tue, 16 Jun 2020 09:39:20: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:39:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:39:21: 46000000 INFO @ Tue, 16 Jun 2020 09:39:22: #2 number of paired peaks: 141 WARNING @ Tue, 16 Jun 2020 09:39:22: Fewer paired peaks (141) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 141 pairs to build model! INFO @ Tue, 16 Jun 2020 09:39:22: start model_add_line... INFO @ Tue, 16 Jun 2020 09:39:22: start X-correlation... INFO @ Tue, 16 Jun 2020 09:39:22: end of X-cor INFO @ Tue, 16 Jun 2020 09:39:22: #2 finished! INFO @ Tue, 16 Jun 2020 09:39:22: #2 predicted fragment length is 56 bps INFO @ Tue, 16 Jun 2020 09:39:22: #2 alternative fragment length(s) may be 1,56,95,112,136 bps INFO @ Tue, 16 Jun 2020 09:39:22: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4085359/SRX4085359.10_model.r WARNING @ Tue, 16 Jun 2020 09:39:22: #2 Since the d (56) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 09:39:22: #2 You may need to consider one of the other alternative d(s): 1,56,95,112,136 WARNING @ Tue, 16 Jun 2020 09:39:22: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 09:39:22: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:39:22: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:39:23: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 09:39:25: 47000000 INFO @ Tue, 16 Jun 2020 09:39:30: 48000000 INFO @ Tue, 16 Jun 2020 09:39:35: 49000000 INFO @ Tue, 16 Jun 2020 09:39:39: 50000000 INFO @ Tue, 16 Jun 2020 09:39:40: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4085359/SRX4085359.05_peaks.xls INFO @ Tue, 16 Jun 2020 09:39:40: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4085359/SRX4085359.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:39:40: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4085359/SRX4085359.05_summits.bed INFO @ Tue, 16 Jun 2020 09:39:40: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (2016 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:39:44: 51000000 INFO @ Tue, 16 Jun 2020 09:39:48: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 09:39:48: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 09:39:48: #1 total tags in treatment: 25273748 INFO @ Tue, 16 Jun 2020 09:39:48: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:39:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:39:48: #1 tags after filtering in treatment: 20085425 INFO @ Tue, 16 Jun 2020 09:39:48: #1 Redundant rate of treatment: 0.21 INFO @ Tue, 16 Jun 2020 09:39:48: #1 finished! INFO @ Tue, 16 Jun 2020 09:39:48: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:39:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:39:49: #2 number of paired peaks: 141 WARNING @ Tue, 16 Jun 2020 09:39:49: Fewer paired peaks (141) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 141 pairs to build model! INFO @ Tue, 16 Jun 2020 09:39:49: start model_add_line... INFO @ Tue, 16 Jun 2020 09:39:50: start X-correlation... INFO @ Tue, 16 Jun 2020 09:39:50: end of X-cor INFO @ Tue, 16 Jun 2020 09:39:50: #2 finished! INFO @ Tue, 16 Jun 2020 09:39:50: #2 predicted fragment length is 56 bps INFO @ Tue, 16 Jun 2020 09:39:50: #2 alternative fragment length(s) may be 1,56,95,112,136 bps INFO @ Tue, 16 Jun 2020 09:39:50: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4085359/SRX4085359.20_model.r WARNING @ Tue, 16 Jun 2020 09:39:50: #2 Since the d (56) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 09:39:50: #2 You may need to consider one of the other alternative d(s): 1,56,95,112,136 WARNING @ Tue, 16 Jun 2020 09:39:50: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 09:39:50: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:39:50: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:39:56: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:40:13: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4085359/SRX4085359.10_peaks.xls INFO @ Tue, 16 Jun 2020 09:40:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4085359/SRX4085359.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:40:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4085359/SRX4085359.10_summits.bed INFO @ Tue, 16 Jun 2020 09:40:13: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (762 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:40:25: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 09:40:41: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4085359/SRX4085359.20_peaks.xls INFO @ Tue, 16 Jun 2020 09:40:41: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4085359/SRX4085359.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:40:41: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4085359/SRX4085359.20_summits.bed INFO @ Tue, 16 Jun 2020 09:40:41: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (122 records, 4 fields): 1 millis CompletedMACS2peakCalling