Job ID = 12265516 SRX = SRX4082390 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:50:18 68043985 reads; of these: 68043985 (100.00%) were paired; of these: 6413506 (9.43%) aligned concordantly 0 times 55489692 (81.55%) aligned concordantly exactly 1 time 6140787 (9.02%) aligned concordantly >1 times ---- 6413506 pairs aligned concordantly 0 times; of these: 3026562 (47.19%) aligned discordantly 1 time ---- 3386944 pairs aligned 0 times concordantly or discordantly; of these: 6773888 mates make up the pairs; of these: 4875428 (71.97%) aligned 0 times 1151943 (17.01%) aligned exactly 1 time 746517 (11.02%) aligned >1 times 96.42% overall alignment rate Time searching: 00:50:18 Overall time: 00:50:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 56 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 26696952 / 62057457 = 0.4302 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:44:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4082390/SRX4082390.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4082390/SRX4082390.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4082390/SRX4082390.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4082390/SRX4082390.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:44:54: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:44:54: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:45:01: 1000000 INFO @ Sat, 03 Apr 2021 08:45:07: 2000000 INFO @ Sat, 03 Apr 2021 08:45:14: 3000000 INFO @ Sat, 03 Apr 2021 08:45:21: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:45:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4082390/SRX4082390.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4082390/SRX4082390.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4082390/SRX4082390.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4082390/SRX4082390.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:45:24: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:45:24: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:45:28: 5000000 INFO @ Sat, 03 Apr 2021 08:45:32: 1000000 INFO @ Sat, 03 Apr 2021 08:45:35: 6000000 INFO @ Sat, 03 Apr 2021 08:45:39: 2000000 INFO @ Sat, 03 Apr 2021 08:45:43: 7000000 INFO @ Sat, 03 Apr 2021 08:45:46: 3000000 INFO @ Sat, 03 Apr 2021 08:45:51: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:45:54: 4000000 INFO @ Sat, 03 Apr 2021 08:45:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4082390/SRX4082390.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4082390/SRX4082390.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4082390/SRX4082390.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4082390/SRX4082390.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:45:54: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:45:54: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:45:58: 9000000 INFO @ Sat, 03 Apr 2021 08:46:00: 1000000 INFO @ Sat, 03 Apr 2021 08:46:02: 5000000 INFO @ Sat, 03 Apr 2021 08:46:06: 10000000 INFO @ Sat, 03 Apr 2021 08:46:07: 2000000 INFO @ Sat, 03 Apr 2021 08:46:10: 6000000 INFO @ Sat, 03 Apr 2021 08:46:13: 3000000 INFO @ Sat, 03 Apr 2021 08:46:14: 11000000 INFO @ Sat, 03 Apr 2021 08:46:18: 7000000 INFO @ Sat, 03 Apr 2021 08:46:19: 4000000 INFO @ Sat, 03 Apr 2021 08:46:22: 12000000 INFO @ Sat, 03 Apr 2021 08:46:25: 8000000 INFO @ Sat, 03 Apr 2021 08:46:26: 5000000 INFO @ Sat, 03 Apr 2021 08:46:30: 13000000 INFO @ Sat, 03 Apr 2021 08:46:32: 6000000 INFO @ Sat, 03 Apr 2021 08:46:33: 9000000 INFO @ Sat, 03 Apr 2021 08:46:38: 14000000 INFO @ Sat, 03 Apr 2021 08:46:38: 7000000 INFO @ Sat, 03 Apr 2021 08:46:41: 10000000 INFO @ Sat, 03 Apr 2021 08:46:44: 8000000 INFO @ Sat, 03 Apr 2021 08:46:45: 15000000 INFO @ Sat, 03 Apr 2021 08:46:49: 11000000 INFO @ Sat, 03 Apr 2021 08:46:51: 9000000 INFO @ Sat, 03 Apr 2021 08:46:53: 16000000 INFO @ Sat, 03 Apr 2021 08:46:57: 12000000 INFO @ Sat, 03 Apr 2021 08:46:57: 10000000 INFO @ Sat, 03 Apr 2021 08:47:01: 17000000 INFO @ Sat, 03 Apr 2021 08:47:04: 11000000 INFO @ Sat, 03 Apr 2021 08:47:05: 13000000 INFO @ Sat, 03 Apr 2021 08:47:09: 18000000 INFO @ Sat, 03 Apr 2021 08:47:10: 12000000 INFO @ Sat, 03 Apr 2021 08:47:13: 14000000 INFO @ Sat, 03 Apr 2021 08:47:16: 13000000 INFO @ Sat, 03 Apr 2021 08:47:16: 19000000 INFO @ Sat, 03 Apr 2021 08:47:20: 15000000 INFO @ Sat, 03 Apr 2021 08:47:22: 14000000 INFO @ Sat, 03 Apr 2021 08:47:24: 20000000 INFO @ Sat, 03 Apr 2021 08:47:28: 16000000 INFO @ Sat, 03 Apr 2021 08:47:28: 15000000 INFO @ Sat, 03 Apr 2021 08:47:32: 21000000 INFO @ Sat, 03 Apr 2021 08:47:34: 16000000 INFO @ Sat, 03 Apr 2021 08:47:36: 17000000 INFO @ Sat, 03 Apr 2021 08:47:40: 22000000 INFO @ Sat, 03 Apr 2021 08:47:41: 17000000 INFO @ Sat, 03 Apr 2021 08:47:44: 18000000 INFO @ Sat, 03 Apr 2021 08:47:47: 18000000 INFO @ Sat, 03 Apr 2021 08:47:48: 23000000 INFO @ Sat, 03 Apr 2021 08:47:52: 19000000 INFO @ Sat, 03 Apr 2021 08:47:53: 19000000 INFO @ Sat, 03 Apr 2021 08:47:55: 24000000 INFO @ Sat, 03 Apr 2021 08:47:59: 20000000 INFO @ Sat, 03 Apr 2021 08:47:59: 20000000 INFO @ Sat, 03 Apr 2021 08:48:03: 25000000 INFO @ Sat, 03 Apr 2021 08:48:06: 21000000 INFO @ Sat, 03 Apr 2021 08:48:07: 21000000 INFO @ Sat, 03 Apr 2021 08:48:11: 26000000 INFO @ Sat, 03 Apr 2021 08:48:12: 22000000 INFO @ Sat, 03 Apr 2021 08:48:14: 22000000 INFO @ Sat, 03 Apr 2021 08:48:18: 23000000 INFO @ Sat, 03 Apr 2021 08:48:18: 27000000 INFO @ Sat, 03 Apr 2021 08:48:22: 23000000 INFO @ Sat, 03 Apr 2021 08:48:24: 24000000 INFO @ Sat, 03 Apr 2021 08:48:26: 28000000 INFO @ Sat, 03 Apr 2021 08:48:30: 24000000 INFO @ Sat, 03 Apr 2021 08:48:31: 25000000 INFO @ Sat, 03 Apr 2021 08:48:33: 29000000 INFO @ Sat, 03 Apr 2021 08:48:37: 26000000 INFO @ Sat, 03 Apr 2021 08:48:38: 25000000 INFO @ Sat, 03 Apr 2021 08:48:42: 30000000 INFO @ Sat, 03 Apr 2021 08:48:44: 27000000 INFO @ Sat, 03 Apr 2021 08:48:46: 26000000 INFO @ Sat, 03 Apr 2021 08:48:49: 31000000 INFO @ Sat, 03 Apr 2021 08:48:50: 28000000 INFO @ Sat, 03 Apr 2021 08:48:53: 27000000 INFO @ Sat, 03 Apr 2021 08:48:56: 29000000 INFO @ Sat, 03 Apr 2021 08:48:57: 32000000 INFO @ Sat, 03 Apr 2021 08:49:01: 28000000 INFO @ Sat, 03 Apr 2021 08:49:02: 30000000 INFO @ Sat, 03 Apr 2021 08:49:05: 33000000 INFO @ Sat, 03 Apr 2021 08:49:08: 29000000 INFO @ Sat, 03 Apr 2021 08:49:08: 31000000 INFO @ Sat, 03 Apr 2021 08:49:13: 34000000 INFO @ Sat, 03 Apr 2021 08:49:14: 32000000 INFO @ Sat, 03 Apr 2021 08:49:16: 30000000 INFO @ Sat, 03 Apr 2021 08:49:20: 35000000 INFO @ Sat, 03 Apr 2021 08:49:21: 33000000 INFO @ Sat, 03 Apr 2021 08:49:24: 31000000 INFO @ Sat, 03 Apr 2021 08:49:27: 34000000 INFO @ Sat, 03 Apr 2021 08:49:28: 36000000 INFO @ Sat, 03 Apr 2021 08:49:31: 32000000 INFO @ Sat, 03 Apr 2021 08:49:33: 35000000 INFO @ Sat, 03 Apr 2021 08:49:35: 37000000 INFO @ Sat, 03 Apr 2021 08:49:39: 33000000 INFO @ Sat, 03 Apr 2021 08:49:39: 36000000 INFO @ Sat, 03 Apr 2021 08:49:43: 38000000 INFO @ Sat, 03 Apr 2021 08:49:46: 37000000 INFO @ Sat, 03 Apr 2021 08:49:46: 34000000 INFO @ Sat, 03 Apr 2021 08:49:50: 39000000 INFO @ Sat, 03 Apr 2021 08:49:52: 38000000 INFO @ Sat, 03 Apr 2021 08:49:54: 35000000 INFO @ Sat, 03 Apr 2021 08:49:58: 40000000 INFO @ Sat, 03 Apr 2021 08:50:00: 39000000 INFO @ Sat, 03 Apr 2021 08:50:01: 36000000 INFO @ Sat, 03 Apr 2021 08:50:06: 41000000 INFO @ Sat, 03 Apr 2021 08:50:07: 40000000 INFO @ Sat, 03 Apr 2021 08:50:10: 37000000 INFO @ Sat, 03 Apr 2021 08:50:13: 42000000 INFO @ Sat, 03 Apr 2021 08:50:15: 41000000 INFO @ Sat, 03 Apr 2021 08:50:17: 38000000 INFO @ Sat, 03 Apr 2021 08:50:21: 43000000 INFO @ Sat, 03 Apr 2021 08:50:23: 42000000 INFO @ Sat, 03 Apr 2021 08:50:25: 39000000 INFO @ Sat, 03 Apr 2021 08:50:29: 44000000 INFO @ Sat, 03 Apr 2021 08:50:31: 43000000 INFO @ Sat, 03 Apr 2021 08:50:33: 40000000 INFO @ Sat, 03 Apr 2021 08:50:37: 45000000 INFO @ Sat, 03 Apr 2021 08:50:39: 44000000 INFO @ Sat, 03 Apr 2021 08:50:41: 41000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 08:50:45: 46000000 INFO @ Sat, 03 Apr 2021 08:50:47: 45000000 INFO @ Sat, 03 Apr 2021 08:50:48: 42000000 INFO @ Sat, 03 Apr 2021 08:50:53: 47000000 INFO @ Sat, 03 Apr 2021 08:50:56: 46000000 INFO @ Sat, 03 Apr 2021 08:50:57: 43000000 INFO @ Sat, 03 Apr 2021 08:51:01: 48000000 INFO @ Sat, 03 Apr 2021 08:51:05: 44000000 INFO @ Sat, 03 Apr 2021 08:51:05: 47000000 INFO @ Sat, 03 Apr 2021 08:51:09: 49000000 INFO @ Sat, 03 Apr 2021 08:51:12: 45000000 INFO @ Sat, 03 Apr 2021 08:51:14: 48000000 INFO @ Sat, 03 Apr 2021 08:51:17: 50000000 INFO @ Sat, 03 Apr 2021 08:51:21: 46000000 INFO @ Sat, 03 Apr 2021 08:51:23: 49000000 INFO @ Sat, 03 Apr 2021 08:51:25: 51000000 INFO @ Sat, 03 Apr 2021 08:51:29: 47000000 INFO @ Sat, 03 Apr 2021 08:51:32: 50000000 INFO @ Sat, 03 Apr 2021 08:51:32: 52000000 INFO @ Sat, 03 Apr 2021 08:51:37: 48000000 INFO @ Sat, 03 Apr 2021 08:51:40: 53000000 INFO @ Sat, 03 Apr 2021 08:51:41: 51000000 INFO @ Sat, 03 Apr 2021 08:51:45: 49000000 INFO @ Sat, 03 Apr 2021 08:51:47: 54000000 INFO @ Sat, 03 Apr 2021 08:51:50: 52000000 INFO @ Sat, 03 Apr 2021 08:51:53: 50000000 INFO @ Sat, 03 Apr 2021 08:51:54: 55000000 INFO @ Sat, 03 Apr 2021 08:51:59: 53000000 INFO @ Sat, 03 Apr 2021 08:52:01: 51000000 INFO @ Sat, 03 Apr 2021 08:52:02: 56000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 08:52:08: 54000000 INFO @ Sat, 03 Apr 2021 08:52:09: 52000000 INFO @ Sat, 03 Apr 2021 08:52:10: 57000000 INFO @ Sat, 03 Apr 2021 08:52:16: 53000000 INFO @ Sat, 03 Apr 2021 08:52:17: 55000000 INFO @ Sat, 03 Apr 2021 08:52:17: 58000000 INFO @ Sat, 03 Apr 2021 08:52:24: 54000000 INFO @ Sat, 03 Apr 2021 08:52:25: 59000000 INFO @ Sat, 03 Apr 2021 08:52:26: 56000000 INFO @ Sat, 03 Apr 2021 08:52:31: 55000000 INFO @ Sat, 03 Apr 2021 08:52:33: 60000000 INFO @ Sat, 03 Apr 2021 08:52:36: 57000000 INFO @ Sat, 03 Apr 2021 08:52:39: 56000000 INFO @ Sat, 03 Apr 2021 08:52:42: 61000000 INFO @ Sat, 03 Apr 2021 08:52:45: 58000000 INFO @ Sat, 03 Apr 2021 08:52:47: 57000000 INFO @ Sat, 03 Apr 2021 08:52:49: 62000000 INFO @ Sat, 03 Apr 2021 08:52:54: 58000000 INFO @ Sat, 03 Apr 2021 08:52:55: 59000000 INFO @ Sat, 03 Apr 2021 08:52:56: 63000000 INFO @ Sat, 03 Apr 2021 08:53:02: 59000000 INFO @ Sat, 03 Apr 2021 08:53:03: 64000000 INFO @ Sat, 03 Apr 2021 08:53:04: 60000000 INFO @ Sat, 03 Apr 2021 08:53:09: 60000000 INFO @ Sat, 03 Apr 2021 08:53:11: 65000000 INFO @ Sat, 03 Apr 2021 08:53:14: 61000000 INFO @ Sat, 03 Apr 2021 08:53:16: 61000000 INFO @ Sat, 03 Apr 2021 08:53:18: 66000000 INFO @ Sat, 03 Apr 2021 08:53:23: 62000000 INFO @ Sat, 03 Apr 2021 08:53:24: 62000000 INFO @ Sat, 03 Apr 2021 08:53:26: 67000000 INFO @ Sat, 03 Apr 2021 08:53:31: 63000000 INFO @ Sat, 03 Apr 2021 08:53:31: 63000000 INFO @ Sat, 03 Apr 2021 08:53:33: 68000000 INFO @ Sat, 03 Apr 2021 08:53:38: 64000000 INFO @ Sat, 03 Apr 2021 08:53:39: 64000000 INFO @ Sat, 03 Apr 2021 08:53:40: 69000000 INFO @ Sat, 03 Apr 2021 08:53:46: 65000000 INFO @ Sat, 03 Apr 2021 08:53:48: 70000000 INFO @ Sat, 03 Apr 2021 08:53:48: 65000000 INFO @ Sat, 03 Apr 2021 08:53:54: 66000000 INFO @ Sat, 03 Apr 2021 08:53:55: 71000000 INFO @ Sat, 03 Apr 2021 08:53:57: 66000000 INFO @ Sat, 03 Apr 2021 08:54:01: 67000000 INFO @ Sat, 03 Apr 2021 08:54:02: 72000000 INFO @ Sat, 03 Apr 2021 08:54:04: 67000000 INFO @ Sat, 03 Apr 2021 08:54:08: 68000000 INFO @ Sat, 03 Apr 2021 08:54:10: 73000000 INFO @ Sat, 03 Apr 2021 08:54:10: 68000000 INFO @ Sat, 03 Apr 2021 08:54:16: 69000000 INFO @ Sat, 03 Apr 2021 08:54:17: 69000000 INFO @ Sat, 03 Apr 2021 08:54:18: 74000000 INFO @ Sat, 03 Apr 2021 08:54:23: 70000000 INFO @ Sat, 03 Apr 2021 08:54:24: 75000000 INFO @ Sat, 03 Apr 2021 08:54:24: 70000000 INFO @ Sat, 03 Apr 2021 08:54:30: 71000000 INFO @ Sat, 03 Apr 2021 08:54:31: 76000000 INFO @ Sat, 03 Apr 2021 08:54:31: 71000000 INFO @ Sat, 03 Apr 2021 08:54:37: 72000000 INFO @ Sat, 03 Apr 2021 08:54:37: 77000000 INFO @ Sat, 03 Apr 2021 08:54:38: 72000000 INFO @ Sat, 03 Apr 2021 08:54:43: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 08:54:43: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 08:54:43: #1 total tags in treatment: 35392192 INFO @ Sat, 03 Apr 2021 08:54:43: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:54:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:54:44: 73000000 INFO @ Sat, 03 Apr 2021 08:54:44: #1 tags after filtering in treatment: 18246000 INFO @ Sat, 03 Apr 2021 08:54:44: #1 Redundant rate of treatment: 0.48 INFO @ Sat, 03 Apr 2021 08:54:44: #1 finished! INFO @ Sat, 03 Apr 2021 08:54:44: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:54:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:54:45: #2 number of paired peaks: 253 WARNING @ Sat, 03 Apr 2021 08:54:45: Fewer paired peaks (253) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 253 pairs to build model! INFO @ Sat, 03 Apr 2021 08:54:45: start model_add_line... INFO @ Sat, 03 Apr 2021 08:54:45: start X-correlation... INFO @ Sat, 03 Apr 2021 08:54:45: end of X-cor INFO @ Sat, 03 Apr 2021 08:54:45: #2 finished! INFO @ Sat, 03 Apr 2021 08:54:45: #2 predicted fragment length is 69 bps INFO @ Sat, 03 Apr 2021 08:54:45: #2 alternative fragment length(s) may be 69 bps INFO @ Sat, 03 Apr 2021 08:54:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4082390/SRX4082390.05_model.r WARNING @ Sat, 03 Apr 2021 08:54:45: #2 Since the d (69) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:54:45: #2 You may need to consider one of the other alternative d(s): 69 WARNING @ Sat, 03 Apr 2021 08:54:45: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:54:45: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:54:45: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:54:46: 73000000 INFO @ Sat, 03 Apr 2021 08:54:51: 74000000 INFO @ Sat, 03 Apr 2021 08:54:53: 74000000 INFO @ Sat, 03 Apr 2021 08:54:57: 75000000 INFO @ Sat, 03 Apr 2021 08:54:59: 75000000 INFO @ Sat, 03 Apr 2021 08:55:03: 76000000 INFO @ Sat, 03 Apr 2021 08:55:06: 76000000 INFO @ Sat, 03 Apr 2021 08:55:09: 77000000 INFO @ Sat, 03 Apr 2021 08:55:13: 77000000 INFO @ Sat, 03 Apr 2021 08:55:14: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 08:55:14: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 08:55:14: #1 total tags in treatment: 35392192 INFO @ Sat, 03 Apr 2021 08:55:14: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:55:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:55:15: #1 tags after filtering in treatment: 18246000 INFO @ Sat, 03 Apr 2021 08:55:15: #1 Redundant rate of treatment: 0.48 INFO @ Sat, 03 Apr 2021 08:55:15: #1 finished! INFO @ Sat, 03 Apr 2021 08:55:15: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:55:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:55:16: #2 number of paired peaks: 253 WARNING @ Sat, 03 Apr 2021 08:55:16: Fewer paired peaks (253) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 253 pairs to build model! INFO @ Sat, 03 Apr 2021 08:55:16: start model_add_line... INFO @ Sat, 03 Apr 2021 08:55:17: start X-correlation... INFO @ Sat, 03 Apr 2021 08:55:17: end of X-cor INFO @ Sat, 03 Apr 2021 08:55:17: #2 finished! INFO @ Sat, 03 Apr 2021 08:55:17: #2 predicted fragment length is 69 bps INFO @ Sat, 03 Apr 2021 08:55:17: #2 alternative fragment length(s) may be 69 bps INFO @ Sat, 03 Apr 2021 08:55:17: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4082390/SRX4082390.10_model.r WARNING @ Sat, 03 Apr 2021 08:55:17: #2 Since the d (69) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:55:17: #2 You may need to consider one of the other alternative d(s): 69 WARNING @ Sat, 03 Apr 2021 08:55:17: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:55:17: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:55:17: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:55:19: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:55:19: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 08:55:19: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 08:55:19: #1 total tags in treatment: 35392192 INFO @ Sat, 03 Apr 2021 08:55:19: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:55:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:55:19: #1 tags after filtering in treatment: 18246000 INFO @ Sat, 03 Apr 2021 08:55:19: #1 Redundant rate of treatment: 0.48 INFO @ Sat, 03 Apr 2021 08:55:19: #1 finished! INFO @ Sat, 03 Apr 2021 08:55:19: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:55:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:55:21: #2 number of paired peaks: 253 WARNING @ Sat, 03 Apr 2021 08:55:21: Fewer paired peaks (253) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 253 pairs to build model! INFO @ Sat, 03 Apr 2021 08:55:21: start model_add_line... INFO @ Sat, 03 Apr 2021 08:55:21: start X-correlation... INFO @ Sat, 03 Apr 2021 08:55:21: end of X-cor INFO @ Sat, 03 Apr 2021 08:55:21: #2 finished! INFO @ Sat, 03 Apr 2021 08:55:21: #2 predicted fragment length is 69 bps INFO @ Sat, 03 Apr 2021 08:55:21: #2 alternative fragment length(s) may be 69 bps INFO @ Sat, 03 Apr 2021 08:55:21: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4082390/SRX4082390.20_model.r WARNING @ Sat, 03 Apr 2021 08:55:21: #2 Since the d (69) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:55:21: #2 You may need to consider one of the other alternative d(s): 69 WARNING @ Sat, 03 Apr 2021 08:55:21: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:55:21: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:55:21: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:55:38: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4082390/SRX4082390.05_peaks.xls INFO @ Sat, 03 Apr 2021 08:55:38: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4082390/SRX4082390.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:55:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4082390/SRX4082390.05_summits.bed INFO @ Sat, 03 Apr 2021 08:55:38: Done! pass1 - making usageList (7 chroms): 3 millis pass2 - checking and writing primary data (14882 records, 4 fields): 18 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:55:50: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:55:54: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:56:08: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4082390/SRX4082390.10_peaks.xls INFO @ Sat, 03 Apr 2021 08:56:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4082390/SRX4082390.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:56:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4082390/SRX4082390.10_summits.bed INFO @ Sat, 03 Apr 2021 08:56:08: Done! pass1 - making usageList (6 chroms): 2 millis pass2 - checking and writing primary data (8650 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:56:12: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4082390/SRX4082390.20_peaks.xls INFO @ Sat, 03 Apr 2021 08:56:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4082390/SRX4082390.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:56:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4082390/SRX4082390.20_summits.bed INFO @ Sat, 03 Apr 2021 08:56:13: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (3318 records, 4 fields): 6 millis CompletedMACS2peakCalling