Job ID = 6367740 SRX = SRX4082354 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:31:20 prefetch.2.10.7: 1) Downloading 'SRR7164172'... 2020-06-15T23:31:20 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:35:50 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:35:50 prefetch.2.10.7: 1) 'SRR7164172' was downloaded successfully 2020-06-15T23:35:50 prefetch.2.10.7: 'SRR7164172' has 0 unresolved dependencies Read 26760587 spots for SRR7164172/SRR7164172.sra Written 26760587 spots for SRR7164172/SRR7164172.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:34 26760587 reads; of these: 26760587 (100.00%) were unpaired; of these: 263908 (0.99%) aligned 0 times 22968993 (85.83%) aligned exactly 1 time 3527686 (13.18%) aligned >1 times 99.01% overall alignment rate Time searching: 00:05:34 Overall time: 00:05:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4681187 / 26496679 = 0.1767 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:50:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4082354/SRX4082354.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4082354/SRX4082354.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4082354/SRX4082354.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4082354/SRX4082354.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:50:48: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:50:48: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:50:53: 1000000 INFO @ Tue, 16 Jun 2020 08:50:58: 2000000 INFO @ Tue, 16 Jun 2020 08:51:02: 3000000 INFO @ Tue, 16 Jun 2020 08:51:07: 4000000 INFO @ Tue, 16 Jun 2020 08:51:12: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:51:16: 6000000 INFO @ Tue, 16 Jun 2020 08:51:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4082354/SRX4082354.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4082354/SRX4082354.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4082354/SRX4082354.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4082354/SRX4082354.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:51:18: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:51:18: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:51:21: 7000000 INFO @ Tue, 16 Jun 2020 08:51:23: 1000000 INFO @ Tue, 16 Jun 2020 08:51:26: 8000000 INFO @ Tue, 16 Jun 2020 08:51:28: 2000000 INFO @ Tue, 16 Jun 2020 08:51:31: 9000000 INFO @ Tue, 16 Jun 2020 08:51:33: 3000000 INFO @ Tue, 16 Jun 2020 08:51:35: 10000000 INFO @ Tue, 16 Jun 2020 08:51:38: 4000000 INFO @ Tue, 16 Jun 2020 08:51:40: 11000000 INFO @ Tue, 16 Jun 2020 08:51:42: 5000000 INFO @ Tue, 16 Jun 2020 08:51:45: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:51:47: 6000000 INFO @ Tue, 16 Jun 2020 08:51:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4082354/SRX4082354.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4082354/SRX4082354.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4082354/SRX4082354.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4082354/SRX4082354.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:51:48: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:51:48: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:51:50: 13000000 INFO @ Tue, 16 Jun 2020 08:51:52: 7000000 INFO @ Tue, 16 Jun 2020 08:51:53: 1000000 INFO @ Tue, 16 Jun 2020 08:51:55: 14000000 INFO @ Tue, 16 Jun 2020 08:51:57: 8000000 INFO @ Tue, 16 Jun 2020 08:51:58: 2000000 INFO @ Tue, 16 Jun 2020 08:52:00: 15000000 INFO @ Tue, 16 Jun 2020 08:52:02: 9000000 INFO @ Tue, 16 Jun 2020 08:52:03: 3000000 INFO @ Tue, 16 Jun 2020 08:52:05: 16000000 INFO @ Tue, 16 Jun 2020 08:52:07: 10000000 INFO @ Tue, 16 Jun 2020 08:52:08: 4000000 INFO @ Tue, 16 Jun 2020 08:52:11: 17000000 INFO @ Tue, 16 Jun 2020 08:52:12: 11000000 INFO @ Tue, 16 Jun 2020 08:52:13: 5000000 INFO @ Tue, 16 Jun 2020 08:52:16: 18000000 INFO @ Tue, 16 Jun 2020 08:52:17: 12000000 INFO @ Tue, 16 Jun 2020 08:52:18: 6000000 INFO @ Tue, 16 Jun 2020 08:52:21: 19000000 INFO @ Tue, 16 Jun 2020 08:52:22: 13000000 INFO @ Tue, 16 Jun 2020 08:52:24: 7000000 INFO @ Tue, 16 Jun 2020 08:52:26: 20000000 INFO @ Tue, 16 Jun 2020 08:52:27: 14000000 INFO @ Tue, 16 Jun 2020 08:52:29: 8000000 INFO @ Tue, 16 Jun 2020 08:52:31: 21000000 INFO @ Tue, 16 Jun 2020 08:52:32: 15000000 INFO @ Tue, 16 Jun 2020 08:52:34: 9000000 INFO @ Tue, 16 Jun 2020 08:52:36: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:52:36: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:52:36: #1 total tags in treatment: 21815492 INFO @ Tue, 16 Jun 2020 08:52:36: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:52:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:52:36: #1 tags after filtering in treatment: 21815492 INFO @ Tue, 16 Jun 2020 08:52:36: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:52:36: #1 finished! INFO @ Tue, 16 Jun 2020 08:52:36: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:52:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:52:37: 16000000 INFO @ Tue, 16 Jun 2020 08:52:37: #2 number of paired peaks: 62 WARNING @ Tue, 16 Jun 2020 08:52:37: Too few paired peaks (62) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:52:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX4082354/SRX4082354.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4082354/SRX4082354.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4082354/SRX4082354.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4082354/SRX4082354.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:52:39: 10000000 INFO @ Tue, 16 Jun 2020 08:52:42: 17000000 INFO @ Tue, 16 Jun 2020 08:52:44: 11000000 INFO @ Tue, 16 Jun 2020 08:52:47: 18000000 INFO @ Tue, 16 Jun 2020 08:52:48: 12000000 INFO @ Tue, 16 Jun 2020 08:52:52: 19000000 INFO @ Tue, 16 Jun 2020 08:52:53: 13000000 INFO @ Tue, 16 Jun 2020 08:52:57: 20000000 INFO @ Tue, 16 Jun 2020 08:52:58: 14000000 INFO @ Tue, 16 Jun 2020 08:53:02: 21000000 INFO @ Tue, 16 Jun 2020 08:53:03: 15000000 INFO @ Tue, 16 Jun 2020 08:53:06: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:53:06: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:53:06: #1 total tags in treatment: 21815492 INFO @ Tue, 16 Jun 2020 08:53:06: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:53:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:53:06: #1 tags after filtering in treatment: 21815492 INFO @ Tue, 16 Jun 2020 08:53:06: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:53:06: #1 finished! INFO @ Tue, 16 Jun 2020 08:53:06: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:53:06: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:53:08: 16000000 INFO @ Tue, 16 Jun 2020 08:53:08: #2 number of paired peaks: 62 WARNING @ Tue, 16 Jun 2020 08:53:08: Too few paired peaks (62) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:53:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX4082354/SRX4082354.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4082354/SRX4082354.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4082354/SRX4082354.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4082354/SRX4082354.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:53:12: 17000000 INFO @ Tue, 16 Jun 2020 08:53:17: 18000000 INFO @ Tue, 16 Jun 2020 08:53:22: 19000000 INFO @ Tue, 16 Jun 2020 08:53:26: 20000000 INFO @ Tue, 16 Jun 2020 08:53:31: 21000000 INFO @ Tue, 16 Jun 2020 08:53:35: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:53:35: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:53:35: #1 total tags in treatment: 21815492 INFO @ Tue, 16 Jun 2020 08:53:35: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:53:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:53:36: #1 tags after filtering in treatment: 21815492 INFO @ Tue, 16 Jun 2020 08:53:36: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:53:36: #1 finished! INFO @ Tue, 16 Jun 2020 08:53:36: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:53:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:53:37: #2 number of paired peaks: 62 WARNING @ Tue, 16 Jun 2020 08:53:37: Too few paired peaks (62) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:53:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX4082354/SRX4082354.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4082354/SRX4082354.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4082354/SRX4082354.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4082354/SRX4082354.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。