Job ID = 6367736 SRX = SRX4082350 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:38:02 prefetch.2.10.7: 1) Downloading 'SRR7164168'... 2020-06-15T23:38:02 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:39:55 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:39:56 prefetch.2.10.7: 'SRR7164168' is valid 2020-06-15T23:39:56 prefetch.2.10.7: 1) 'SRR7164168' was downloaded successfully 2020-06-15T23:39:56 prefetch.2.10.7: 'SRR7164168' has 0 unresolved dependencies Read 13103423 spots for SRR7164168/SRR7164168.sra Written 13103423 spots for SRR7164168/SRR7164168.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:41 13103423 reads; of these: 13103423 (100.00%) were unpaired; of these: 1542541 (11.77%) aligned 0 times 10214242 (77.95%) aligned exactly 1 time 1346640 (10.28%) aligned >1 times 88.23% overall alignment rate Time searching: 00:02:41 Overall time: 00:02:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1378990 / 11560882 = 0.1193 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:46:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4082350/SRX4082350.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4082350/SRX4082350.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4082350/SRX4082350.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4082350/SRX4082350.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:46:22: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:46:22: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:46:28: 1000000 INFO @ Tue, 16 Jun 2020 08:46:33: 2000000 INFO @ Tue, 16 Jun 2020 08:46:38: 3000000 INFO @ Tue, 16 Jun 2020 08:46:44: 4000000 INFO @ Tue, 16 Jun 2020 08:46:49: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:46:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4082350/SRX4082350.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4082350/SRX4082350.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4082350/SRX4082350.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4082350/SRX4082350.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:46:52: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:46:52: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:46:55: 6000000 INFO @ Tue, 16 Jun 2020 08:46:58: 1000000 INFO @ Tue, 16 Jun 2020 08:47:00: 7000000 INFO @ Tue, 16 Jun 2020 08:47:03: 2000000 INFO @ Tue, 16 Jun 2020 08:47:06: 8000000 INFO @ Tue, 16 Jun 2020 08:47:08: 3000000 INFO @ Tue, 16 Jun 2020 08:47:11: 9000000 INFO @ Tue, 16 Jun 2020 08:47:14: 4000000 INFO @ Tue, 16 Jun 2020 08:47:17: 10000000 INFO @ Tue, 16 Jun 2020 08:47:18: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:47:18: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:47:18: #1 total tags in treatment: 10181892 INFO @ Tue, 16 Jun 2020 08:47:18: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:47:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:47:18: #1 tags after filtering in treatment: 10181892 INFO @ Tue, 16 Jun 2020 08:47:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:47:18: #1 finished! INFO @ Tue, 16 Jun 2020 08:47:18: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:47:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:47:19: 5000000 INFO @ Tue, 16 Jun 2020 08:47:19: #2 number of paired peaks: 76 WARNING @ Tue, 16 Jun 2020 08:47:19: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:47:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX4082350/SRX4082350.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4082350/SRX4082350.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4082350/SRX4082350.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4082350/SRX4082350.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:47:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4082350/SRX4082350.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4082350/SRX4082350.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4082350/SRX4082350.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4082350/SRX4082350.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:47:22: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:47:22: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:47:24: 6000000 INFO @ Tue, 16 Jun 2020 08:47:28: 1000000 INFO @ Tue, 16 Jun 2020 08:47:30: 7000000 INFO @ Tue, 16 Jun 2020 08:47:33: 2000000 INFO @ Tue, 16 Jun 2020 08:47:35: 8000000 INFO @ Tue, 16 Jun 2020 08:47:39: 3000000 INFO @ Tue, 16 Jun 2020 08:47:40: 9000000 INFO @ Tue, 16 Jun 2020 08:47:45: 4000000 INFO @ Tue, 16 Jun 2020 08:47:46: 10000000 INFO @ Tue, 16 Jun 2020 08:47:47: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:47:47: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:47:47: #1 total tags in treatment: 10181892 INFO @ Tue, 16 Jun 2020 08:47:47: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:47:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:47:47: #1 tags after filtering in treatment: 10181892 INFO @ Tue, 16 Jun 2020 08:47:47: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:47:47: #1 finished! INFO @ Tue, 16 Jun 2020 08:47:47: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:47:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:47:48: #2 number of paired peaks: 76 WARNING @ Tue, 16 Jun 2020 08:47:48: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:47:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX4082350/SRX4082350.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4082350/SRX4082350.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4082350/SRX4082350.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4082350/SRX4082350.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:47:51: 5000000 INFO @ Tue, 16 Jun 2020 08:47:56: 6000000 INFO @ Tue, 16 Jun 2020 08:48:02: 7000000 INFO @ Tue, 16 Jun 2020 08:48:08: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:48:13: 9000000 INFO @ Tue, 16 Jun 2020 08:48:19: 10000000 INFO @ Tue, 16 Jun 2020 08:48:20: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:48:20: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:48:20: #1 total tags in treatment: 10181892 INFO @ Tue, 16 Jun 2020 08:48:20: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:48:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:48:20: #1 tags after filtering in treatment: 10181892 INFO @ Tue, 16 Jun 2020 08:48:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:48:20: #1 finished! INFO @ Tue, 16 Jun 2020 08:48:20: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:48:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:48:21: #2 number of paired peaks: 76 WARNING @ Tue, 16 Jun 2020 08:48:21: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:48:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX4082350/SRX4082350.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4082350/SRX4082350.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4082350/SRX4082350.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4082350/SRX4082350.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。