Job ID = 6367636 SRX = SRX3583348 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:32:21 prefetch.2.10.7: 1) Downloading 'SRR6494004'... 2020-06-15T23:32:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:39:43 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:39:43 prefetch.2.10.7: 1) 'SRR6494004' was downloaded successfully 2020-06-15T23:39:43 prefetch.2.10.7: 'SRR6494004' has 0 unresolved dependencies Read 43843727 spots for SRR6494004/SRR6494004.sra Written 43843727 spots for SRR6494004/SRR6494004.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:27 43843727 reads; of these: 43843727 (100.00%) were unpaired; of these: 1182319 (2.70%) aligned 0 times 35715272 (81.46%) aligned exactly 1 time 6946136 (15.84%) aligned >1 times 97.30% overall alignment rate Time searching: 00:15:27 Overall time: 00:15:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7429755 / 42661408 = 0.1742 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:07:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3583348/SRX3583348.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3583348/SRX3583348.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3583348/SRX3583348.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3583348/SRX3583348.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:07:16: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:07:16: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:07:21: 1000000 INFO @ Tue, 16 Jun 2020 09:07:26: 2000000 INFO @ Tue, 16 Jun 2020 09:07:32: 3000000 INFO @ Tue, 16 Jun 2020 09:07:37: 4000000 INFO @ Tue, 16 Jun 2020 09:07:42: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:07:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3583348/SRX3583348.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3583348/SRX3583348.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3583348/SRX3583348.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3583348/SRX3583348.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:07:46: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:07:46: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:07:47: 6000000 INFO @ Tue, 16 Jun 2020 09:07:53: 7000000 INFO @ Tue, 16 Jun 2020 09:07:53: 1000000 INFO @ Tue, 16 Jun 2020 09:07:59: 8000000 INFO @ Tue, 16 Jun 2020 09:08:00: 2000000 INFO @ Tue, 16 Jun 2020 09:08:05: 9000000 INFO @ Tue, 16 Jun 2020 09:08:07: 3000000 INFO @ Tue, 16 Jun 2020 09:08:10: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:08:14: 4000000 INFO @ Tue, 16 Jun 2020 09:08:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3583348/SRX3583348.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3583348/SRX3583348.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3583348/SRX3583348.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3583348/SRX3583348.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:08:16: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:08:16: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:08:16: 11000000 INFO @ Tue, 16 Jun 2020 09:08:21: 5000000 INFO @ Tue, 16 Jun 2020 09:08:22: 1000000 INFO @ Tue, 16 Jun 2020 09:08:22: 12000000 INFO @ Tue, 16 Jun 2020 09:08:28: 2000000 INFO @ Tue, 16 Jun 2020 09:08:28: 13000000 INFO @ Tue, 16 Jun 2020 09:08:28: 6000000 INFO @ Tue, 16 Jun 2020 09:08:34: 14000000 INFO @ Tue, 16 Jun 2020 09:08:34: 3000000 INFO @ Tue, 16 Jun 2020 09:08:36: 7000000 INFO @ Tue, 16 Jun 2020 09:08:40: 15000000 INFO @ Tue, 16 Jun 2020 09:08:40: 4000000 INFO @ Tue, 16 Jun 2020 09:08:43: 8000000 INFO @ Tue, 16 Jun 2020 09:08:46: 5000000 INFO @ Tue, 16 Jun 2020 09:08:46: 16000000 INFO @ Tue, 16 Jun 2020 09:08:50: 9000000 INFO @ Tue, 16 Jun 2020 09:08:52: 6000000 INFO @ Tue, 16 Jun 2020 09:08:52: 17000000 INFO @ Tue, 16 Jun 2020 09:08:57: 10000000 INFO @ Tue, 16 Jun 2020 09:08:58: 7000000 INFO @ Tue, 16 Jun 2020 09:08:58: 18000000 INFO @ Tue, 16 Jun 2020 09:09:04: 8000000 INFO @ Tue, 16 Jun 2020 09:09:04: 19000000 INFO @ Tue, 16 Jun 2020 09:09:04: 11000000 INFO @ Tue, 16 Jun 2020 09:09:10: 9000000 INFO @ Tue, 16 Jun 2020 09:09:10: 20000000 INFO @ Tue, 16 Jun 2020 09:09:11: 12000000 INFO @ Tue, 16 Jun 2020 09:09:16: 10000000 INFO @ Tue, 16 Jun 2020 09:09:16: 21000000 INFO @ Tue, 16 Jun 2020 09:09:18: 13000000 INFO @ Tue, 16 Jun 2020 09:09:22: 11000000 INFO @ Tue, 16 Jun 2020 09:09:22: 22000000 INFO @ Tue, 16 Jun 2020 09:09:26: 14000000 INFO @ Tue, 16 Jun 2020 09:09:27: 12000000 INFO @ Tue, 16 Jun 2020 09:09:28: 23000000 INFO @ Tue, 16 Jun 2020 09:09:33: 15000000 INFO @ Tue, 16 Jun 2020 09:09:33: 13000000 INFO @ Tue, 16 Jun 2020 09:09:34: 24000000 INFO @ Tue, 16 Jun 2020 09:09:39: 14000000 INFO @ Tue, 16 Jun 2020 09:09:40: 25000000 INFO @ Tue, 16 Jun 2020 09:09:40: 16000000 INFO @ Tue, 16 Jun 2020 09:09:45: 15000000 INFO @ Tue, 16 Jun 2020 09:09:46: 26000000 INFO @ Tue, 16 Jun 2020 09:09:48: 17000000 INFO @ Tue, 16 Jun 2020 09:09:51: 16000000 INFO @ Tue, 16 Jun 2020 09:09:52: 27000000 INFO @ Tue, 16 Jun 2020 09:09:55: 18000000 INFO @ Tue, 16 Jun 2020 09:09:57: 17000000 INFO @ Tue, 16 Jun 2020 09:09:58: 28000000 INFO @ Tue, 16 Jun 2020 09:10:03: 19000000 INFO @ Tue, 16 Jun 2020 09:10:03: 18000000 INFO @ Tue, 16 Jun 2020 09:10:04: 29000000 INFO @ Tue, 16 Jun 2020 09:10:09: 19000000 INFO @ Tue, 16 Jun 2020 09:10:10: 20000000 INFO @ Tue, 16 Jun 2020 09:10:10: 30000000 INFO @ Tue, 16 Jun 2020 09:10:15: 20000000 INFO @ Tue, 16 Jun 2020 09:10:16: 31000000 INFO @ Tue, 16 Jun 2020 09:10:17: 21000000 INFO @ Tue, 16 Jun 2020 09:10:21: 21000000 INFO @ Tue, 16 Jun 2020 09:10:22: 32000000 INFO @ Tue, 16 Jun 2020 09:10:25: 22000000 INFO @ Tue, 16 Jun 2020 09:10:27: 22000000 INFO @ Tue, 16 Jun 2020 09:10:29: 33000000 INFO @ Tue, 16 Jun 2020 09:10:32: 23000000 INFO @ Tue, 16 Jun 2020 09:10:33: 23000000 INFO @ Tue, 16 Jun 2020 09:10:35: 34000000 INFO @ Tue, 16 Jun 2020 09:10:40: 24000000 INFO @ Tue, 16 Jun 2020 09:10:40: 24000000 INFO @ Tue, 16 Jun 2020 09:10:41: 35000000 INFO @ Tue, 16 Jun 2020 09:10:43: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:10:43: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:10:43: #1 total tags in treatment: 35231653 INFO @ Tue, 16 Jun 2020 09:10:43: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:10:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:10:44: #1 tags after filtering in treatment: 35231653 INFO @ Tue, 16 Jun 2020 09:10:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:10:44: #1 finished! INFO @ Tue, 16 Jun 2020 09:10:44: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:10:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:10:46: #2 number of paired peaks: 13 WARNING @ Tue, 16 Jun 2020 09:10:46: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:10:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3583348/SRX3583348.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583348/SRX3583348.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583348/SRX3583348.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583348/SRX3583348.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:10:47: 25000000 INFO @ Tue, 16 Jun 2020 09:10:48: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:10:55: 26000000 INFO @ Tue, 16 Jun 2020 09:10:56: 26000000 INFO @ Tue, 16 Jun 2020 09:11:03: 27000000 INFO @ Tue, 16 Jun 2020 09:11:03: 27000000 INFO @ Tue, 16 Jun 2020 09:11:12: 28000000 INFO @ Tue, 16 Jun 2020 09:11:12: 28000000 INFO @ Tue, 16 Jun 2020 09:11:18: 29000000 INFO @ Tue, 16 Jun 2020 09:11:20: 29000000 INFO @ Tue, 16 Jun 2020 09:11:24: 30000000 INFO @ Tue, 16 Jun 2020 09:11:27: 30000000 INFO @ Tue, 16 Jun 2020 09:11:30: 31000000 INFO @ Tue, 16 Jun 2020 09:11:34: 31000000 INFO @ Tue, 16 Jun 2020 09:11:36: 32000000 INFO @ Tue, 16 Jun 2020 09:11:42: 32000000 INFO @ Tue, 16 Jun 2020 09:11:42: 33000000 INFO @ Tue, 16 Jun 2020 09:11:48: 34000000 INFO @ Tue, 16 Jun 2020 09:11:49: 33000000 INFO @ Tue, 16 Jun 2020 09:11:54: 35000000 INFO @ Tue, 16 Jun 2020 09:11:56: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:11:56: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:11:56: #1 total tags in treatment: 35231653 INFO @ Tue, 16 Jun 2020 09:11:56: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:11:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:11:56: 34000000 INFO @ Tue, 16 Jun 2020 09:11:57: #1 tags after filtering in treatment: 35231653 INFO @ Tue, 16 Jun 2020 09:11:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:11:57: #1 finished! INFO @ Tue, 16 Jun 2020 09:11:57: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:11:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:11:59: #2 number of paired peaks: 13 WARNING @ Tue, 16 Jun 2020 09:11:59: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:11:59: Process for pairing-model is terminated! BigWig に変換しました。 cut: /home/okishinya/chipatlas/results/ce11/SRX3583348/SRX3583348.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583348/SRX3583348.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583348/SRX3583348.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583348/SRX3583348.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:12:03: 35000000 INFO @ Tue, 16 Jun 2020 09:12:05: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:12:05: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:12:05: #1 total tags in treatment: 35231653 INFO @ Tue, 16 Jun 2020 09:12:05: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:12:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:12:06: #1 tags after filtering in treatment: 35231653 INFO @ Tue, 16 Jun 2020 09:12:06: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:12:06: #1 finished! INFO @ Tue, 16 Jun 2020 09:12:06: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:12:06: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:12:08: #2 number of paired peaks: 13 WARNING @ Tue, 16 Jun 2020 09:12:08: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:12:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3583348/SRX3583348.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583348/SRX3583348.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583348/SRX3583348.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583348/SRX3583348.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling