Job ID = 6367627 SRX = SRX3583339 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:31:20 prefetch.2.10.7: 1) Downloading 'SRR6493995'... 2020-06-15T23:31:20 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:38:01 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:38:01 prefetch.2.10.7: 1) 'SRR6493995' was downloaded successfully 2020-06-15T23:38:01 prefetch.2.10.7: 'SRR6493995' has 0 unresolved dependencies Read 41524876 spots for SRR6493995/SRR6493995.sra Written 41524876 spots for SRR6493995/SRR6493995.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:17 41524876 reads; of these: 41524876 (100.00%) were unpaired; of these: 1293368 (3.11%) aligned 0 times 34819363 (83.85%) aligned exactly 1 time 5412145 (13.03%) aligned >1 times 96.89% overall alignment rate Time searching: 00:13:18 Overall time: 00:13:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8671731 / 40231508 = 0.2155 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:02:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3583339/SRX3583339.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3583339/SRX3583339.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3583339/SRX3583339.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3583339/SRX3583339.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:02:33: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:02:33: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:02:38: 1000000 INFO @ Tue, 16 Jun 2020 09:02:44: 2000000 INFO @ Tue, 16 Jun 2020 09:02:50: 3000000 INFO @ Tue, 16 Jun 2020 09:02:55: 4000000 INFO @ Tue, 16 Jun 2020 09:03:01: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:03:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3583339/SRX3583339.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3583339/SRX3583339.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3583339/SRX3583339.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3583339/SRX3583339.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:03:03: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:03:03: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:03:06: 6000000 INFO @ Tue, 16 Jun 2020 09:03:09: 1000000 INFO @ Tue, 16 Jun 2020 09:03:12: 7000000 INFO @ Tue, 16 Jun 2020 09:03:15: 2000000 INFO @ Tue, 16 Jun 2020 09:03:18: 8000000 INFO @ Tue, 16 Jun 2020 09:03:21: 3000000 INFO @ Tue, 16 Jun 2020 09:03:24: 9000000 INFO @ Tue, 16 Jun 2020 09:03:27: 4000000 INFO @ Tue, 16 Jun 2020 09:03:30: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:03:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3583339/SRX3583339.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3583339/SRX3583339.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3583339/SRX3583339.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3583339/SRX3583339.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:03:33: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:03:33: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:03:33: 5000000 INFO @ Tue, 16 Jun 2020 09:03:36: 11000000 INFO @ Tue, 16 Jun 2020 09:03:39: 1000000 INFO @ Tue, 16 Jun 2020 09:03:39: 6000000 INFO @ Tue, 16 Jun 2020 09:03:42: 12000000 INFO @ Tue, 16 Jun 2020 09:03:45: 2000000 INFO @ Tue, 16 Jun 2020 09:03:45: 7000000 INFO @ Tue, 16 Jun 2020 09:03:48: 13000000 INFO @ Tue, 16 Jun 2020 09:03:51: 3000000 INFO @ Tue, 16 Jun 2020 09:03:51: 8000000 INFO @ Tue, 16 Jun 2020 09:03:54: 14000000 INFO @ Tue, 16 Jun 2020 09:03:57: 4000000 INFO @ Tue, 16 Jun 2020 09:03:58: 9000000 INFO @ Tue, 16 Jun 2020 09:04:00: 15000000 INFO @ Tue, 16 Jun 2020 09:04:04: 5000000 INFO @ Tue, 16 Jun 2020 09:04:04: 10000000 INFO @ Tue, 16 Jun 2020 09:04:07: 16000000 INFO @ Tue, 16 Jun 2020 09:04:10: 6000000 INFO @ Tue, 16 Jun 2020 09:04:10: 11000000 INFO @ Tue, 16 Jun 2020 09:04:13: 17000000 INFO @ Tue, 16 Jun 2020 09:04:16: 7000000 INFO @ Tue, 16 Jun 2020 09:04:17: 12000000 INFO @ Tue, 16 Jun 2020 09:04:19: 18000000 INFO @ Tue, 16 Jun 2020 09:04:23: 8000000 INFO @ Tue, 16 Jun 2020 09:04:23: 13000000 INFO @ Tue, 16 Jun 2020 09:04:25: 19000000 INFO @ Tue, 16 Jun 2020 09:04:29: 9000000 INFO @ Tue, 16 Jun 2020 09:04:29: 14000000 INFO @ Tue, 16 Jun 2020 09:04:31: 20000000 INFO @ Tue, 16 Jun 2020 09:04:35: 10000000 INFO @ Tue, 16 Jun 2020 09:04:35: 15000000 INFO @ Tue, 16 Jun 2020 09:04:37: 21000000 INFO @ Tue, 16 Jun 2020 09:04:41: 11000000 INFO @ Tue, 16 Jun 2020 09:04:41: 16000000 INFO @ Tue, 16 Jun 2020 09:04:43: 22000000 INFO @ Tue, 16 Jun 2020 09:04:47: 12000000 INFO @ Tue, 16 Jun 2020 09:04:47: 17000000 INFO @ Tue, 16 Jun 2020 09:04:49: 23000000 INFO @ Tue, 16 Jun 2020 09:04:53: 13000000 INFO @ Tue, 16 Jun 2020 09:04:53: 18000000 INFO @ Tue, 16 Jun 2020 09:04:54: 24000000 INFO @ Tue, 16 Jun 2020 09:04:59: 14000000 INFO @ Tue, 16 Jun 2020 09:05:00: 19000000 INFO @ Tue, 16 Jun 2020 09:05:00: 25000000 INFO @ Tue, 16 Jun 2020 09:05:05: 15000000 INFO @ Tue, 16 Jun 2020 09:05:06: 20000000 INFO @ Tue, 16 Jun 2020 09:05:06: 26000000 INFO @ Tue, 16 Jun 2020 09:05:11: 16000000 INFO @ Tue, 16 Jun 2020 09:05:12: 21000000 INFO @ Tue, 16 Jun 2020 09:05:12: 27000000 INFO @ Tue, 16 Jun 2020 09:05:17: 17000000 INFO @ Tue, 16 Jun 2020 09:05:18: 22000000 INFO @ Tue, 16 Jun 2020 09:05:18: 28000000 INFO @ Tue, 16 Jun 2020 09:05:24: 18000000 INFO @ Tue, 16 Jun 2020 09:05:24: 23000000 INFO @ Tue, 16 Jun 2020 09:05:25: 29000000 INFO @ Tue, 16 Jun 2020 09:05:30: 19000000 INFO @ Tue, 16 Jun 2020 09:05:30: 24000000 INFO @ Tue, 16 Jun 2020 09:05:31: 30000000 INFO @ Tue, 16 Jun 2020 09:05:36: 20000000 INFO @ Tue, 16 Jun 2020 09:05:37: 25000000 INFO @ Tue, 16 Jun 2020 09:05:37: 31000000 INFO @ Tue, 16 Jun 2020 09:05:40: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:05:40: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:05:40: #1 total tags in treatment: 31559777 INFO @ Tue, 16 Jun 2020 09:05:40: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:05:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:05:41: #1 tags after filtering in treatment: 31559777 INFO @ Tue, 16 Jun 2020 09:05:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:05:41: #1 finished! INFO @ Tue, 16 Jun 2020 09:05:41: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:05:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:05:42: 21000000 INFO @ Tue, 16 Jun 2020 09:05:43: 26000000 INFO @ Tue, 16 Jun 2020 09:05:43: #2 number of paired peaks: 39 WARNING @ Tue, 16 Jun 2020 09:05:43: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:05:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3583339/SRX3583339.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583339/SRX3583339.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583339/SRX3583339.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583339/SRX3583339.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:05:48: 22000000 INFO @ Tue, 16 Jun 2020 09:05:49: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:05:54: 23000000 INFO @ Tue, 16 Jun 2020 09:05:55: 28000000 INFO @ Tue, 16 Jun 2020 09:06:00: 24000000 INFO @ Tue, 16 Jun 2020 09:06:01: 29000000 INFO @ Tue, 16 Jun 2020 09:06:06: 25000000 INFO @ Tue, 16 Jun 2020 09:06:07: 30000000 INFO @ Tue, 16 Jun 2020 09:06:12: 26000000 INFO @ Tue, 16 Jun 2020 09:06:13: 31000000 INFO @ Tue, 16 Jun 2020 09:06:16: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:06:16: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:06:16: #1 total tags in treatment: 31559777 INFO @ Tue, 16 Jun 2020 09:06:16: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:06:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:06:17: #1 tags after filtering in treatment: 31559777 INFO @ Tue, 16 Jun 2020 09:06:17: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:06:17: #1 finished! INFO @ Tue, 16 Jun 2020 09:06:17: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:06:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:06:18: 27000000 INFO @ Tue, 16 Jun 2020 09:06:19: #2 number of paired peaks: 39 WARNING @ Tue, 16 Jun 2020 09:06:19: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:06:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3583339/SRX3583339.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583339/SRX3583339.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583339/SRX3583339.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583339/SRX3583339.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:06:23: 28000000 INFO @ Tue, 16 Jun 2020 09:06:29: 29000000 INFO @ Tue, 16 Jun 2020 09:06:35: 30000000 INFO @ Tue, 16 Jun 2020 09:06:40: 31000000 INFO @ Tue, 16 Jun 2020 09:06:44: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:06:44: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:06:44: #1 total tags in treatment: 31559777 INFO @ Tue, 16 Jun 2020 09:06:44: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:06:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:06:44: #1 tags after filtering in treatment: 31559777 INFO @ Tue, 16 Jun 2020 09:06:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:06:44: #1 finished! INFO @ Tue, 16 Jun 2020 09:06:44: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:06:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:06:46: #2 number of paired peaks: 39 WARNING @ Tue, 16 Jun 2020 09:06:46: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:06:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3583339/SRX3583339.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583339/SRX3583339.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583339/SRX3583339.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583339/SRX3583339.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。