Job ID = 6367626 SRX = SRX3583338 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:31:05 prefetch.2.10.7: 1) Downloading 'SRR6493994'... 2020-06-15T23:31:05 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:35:54 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:35:54 prefetch.2.10.7: 1) 'SRR6493994' was downloaded successfully 2020-06-15T23:35:54 prefetch.2.10.7: 'SRR6493994' has 0 unresolved dependencies Read 42230977 spots for SRR6493994/SRR6493994.sra Written 42230977 spots for SRR6493994/SRR6493994.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:38 42230977 reads; of these: 42230977 (100.00%) were unpaired; of these: 2932588 (6.94%) aligned 0 times 33979670 (80.46%) aligned exactly 1 time 5318719 (12.59%) aligned >1 times 93.06% overall alignment rate Time searching: 00:13:38 Overall time: 00:13:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8130480 / 39298389 = 0.2069 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:01:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3583338/SRX3583338.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3583338/SRX3583338.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3583338/SRX3583338.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3583338/SRX3583338.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:01:17: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:01:17: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:01:25: 1000000 INFO @ Tue, 16 Jun 2020 09:01:33: 2000000 INFO @ Tue, 16 Jun 2020 09:01:41: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:01:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3583338/SRX3583338.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3583338/SRX3583338.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3583338/SRX3583338.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3583338/SRX3583338.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:01:47: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:01:47: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:01:49: 4000000 INFO @ Tue, 16 Jun 2020 09:01:55: 1000000 INFO @ Tue, 16 Jun 2020 09:01:57: 5000000 INFO @ Tue, 16 Jun 2020 09:02:02: 2000000 INFO @ Tue, 16 Jun 2020 09:02:06: 6000000 INFO @ Tue, 16 Jun 2020 09:02:10: 3000000 INFO @ Tue, 16 Jun 2020 09:02:14: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:02:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3583338/SRX3583338.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3583338/SRX3583338.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3583338/SRX3583338.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3583338/SRX3583338.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:02:17: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:02:17: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:02:18: 4000000 INFO @ Tue, 16 Jun 2020 09:02:23: 8000000 INFO @ Tue, 16 Jun 2020 09:02:25: 1000000 INFO @ Tue, 16 Jun 2020 09:02:25: 5000000 INFO @ Tue, 16 Jun 2020 09:02:31: 9000000 INFO @ Tue, 16 Jun 2020 09:02:32: 2000000 INFO @ Tue, 16 Jun 2020 09:02:33: 6000000 INFO @ Tue, 16 Jun 2020 09:02:40: 10000000 INFO @ Tue, 16 Jun 2020 09:02:40: 3000000 INFO @ Tue, 16 Jun 2020 09:02:41: 7000000 INFO @ Tue, 16 Jun 2020 09:02:48: 4000000 INFO @ Tue, 16 Jun 2020 09:02:48: 8000000 INFO @ Tue, 16 Jun 2020 09:02:49: 11000000 INFO @ Tue, 16 Jun 2020 09:02:55: 5000000 INFO @ Tue, 16 Jun 2020 09:02:56: 9000000 INFO @ Tue, 16 Jun 2020 09:02:58: 12000000 INFO @ Tue, 16 Jun 2020 09:03:03: 6000000 INFO @ Tue, 16 Jun 2020 09:03:03: 10000000 INFO @ Tue, 16 Jun 2020 09:03:06: 13000000 INFO @ Tue, 16 Jun 2020 09:03:11: 7000000 INFO @ Tue, 16 Jun 2020 09:03:11: 11000000 INFO @ Tue, 16 Jun 2020 09:03:15: 14000000 INFO @ Tue, 16 Jun 2020 09:03:18: 8000000 INFO @ Tue, 16 Jun 2020 09:03:19: 12000000 INFO @ Tue, 16 Jun 2020 09:03:24: 15000000 INFO @ Tue, 16 Jun 2020 09:03:26: 9000000 INFO @ Tue, 16 Jun 2020 09:03:26: 13000000 INFO @ Tue, 16 Jun 2020 09:03:33: 16000000 INFO @ Tue, 16 Jun 2020 09:03:33: 10000000 INFO @ Tue, 16 Jun 2020 09:03:34: 14000000 INFO @ Tue, 16 Jun 2020 09:03:41: 11000000 INFO @ Tue, 16 Jun 2020 09:03:42: 15000000 INFO @ Tue, 16 Jun 2020 09:03:42: 17000000 INFO @ Tue, 16 Jun 2020 09:03:49: 12000000 INFO @ Tue, 16 Jun 2020 09:03:49: 16000000 INFO @ Tue, 16 Jun 2020 09:03:51: 18000000 INFO @ Tue, 16 Jun 2020 09:03:56: 13000000 INFO @ Tue, 16 Jun 2020 09:03:57: 17000000 INFO @ Tue, 16 Jun 2020 09:03:59: 19000000 INFO @ Tue, 16 Jun 2020 09:04:04: 14000000 INFO @ Tue, 16 Jun 2020 09:04:04: 18000000 INFO @ Tue, 16 Jun 2020 09:04:08: 20000000 INFO @ Tue, 16 Jun 2020 09:04:12: 15000000 INFO @ Tue, 16 Jun 2020 09:04:12: 19000000 INFO @ Tue, 16 Jun 2020 09:04:17: 21000000 INFO @ Tue, 16 Jun 2020 09:04:19: 16000000 INFO @ Tue, 16 Jun 2020 09:04:20: 20000000 INFO @ Tue, 16 Jun 2020 09:04:26: 22000000 INFO @ Tue, 16 Jun 2020 09:04:27: 17000000 INFO @ Tue, 16 Jun 2020 09:04:27: 21000000 INFO @ Tue, 16 Jun 2020 09:04:34: 18000000 INFO @ Tue, 16 Jun 2020 09:04:34: 23000000 INFO @ Tue, 16 Jun 2020 09:04:35: 22000000 INFO @ Tue, 16 Jun 2020 09:04:42: 19000000 INFO @ Tue, 16 Jun 2020 09:04:43: 23000000 INFO @ Tue, 16 Jun 2020 09:04:43: 24000000 INFO @ Tue, 16 Jun 2020 09:04:50: 20000000 INFO @ Tue, 16 Jun 2020 09:04:50: 24000000 INFO @ Tue, 16 Jun 2020 09:04:52: 25000000 INFO @ Tue, 16 Jun 2020 09:04:57: 21000000 INFO @ Tue, 16 Jun 2020 09:04:58: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:05:01: 26000000 INFO @ Tue, 16 Jun 2020 09:05:05: 22000000 INFO @ Tue, 16 Jun 2020 09:05:06: 26000000 INFO @ Tue, 16 Jun 2020 09:05:10: 27000000 INFO @ Tue, 16 Jun 2020 09:05:13: 23000000 INFO @ Tue, 16 Jun 2020 09:05:13: 27000000 INFO @ Tue, 16 Jun 2020 09:05:19: 28000000 INFO @ Tue, 16 Jun 2020 09:05:20: 24000000 INFO @ Tue, 16 Jun 2020 09:05:21: 28000000 INFO @ Tue, 16 Jun 2020 09:05:28: 29000000 INFO @ Tue, 16 Jun 2020 09:05:28: 25000000 INFO @ Tue, 16 Jun 2020 09:05:29: 29000000 INFO @ Tue, 16 Jun 2020 09:05:36: 26000000 INFO @ Tue, 16 Jun 2020 09:05:37: 30000000 INFO @ Tue, 16 Jun 2020 09:05:38: 30000000 INFO @ Tue, 16 Jun 2020 09:05:44: 27000000 INFO @ Tue, 16 Jun 2020 09:05:47: 31000000 INFO @ Tue, 16 Jun 2020 09:05:47: 31000000 INFO @ Tue, 16 Jun 2020 09:05:49: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:05:49: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:05:49: #1 total tags in treatment: 31167909 INFO @ Tue, 16 Jun 2020 09:05:49: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:05:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:05:49: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:05:49: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:05:49: #1 total tags in treatment: 31167909 INFO @ Tue, 16 Jun 2020 09:05:49: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:05:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:05:49: #1 tags after filtering in treatment: 31167909 INFO @ Tue, 16 Jun 2020 09:05:49: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:05:49: #1 finished! INFO @ Tue, 16 Jun 2020 09:05:49: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:05:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:05:49: #1 tags after filtering in treatment: 31167909 INFO @ Tue, 16 Jun 2020 09:05:49: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:05:49: #1 finished! INFO @ Tue, 16 Jun 2020 09:05:49: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:05:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:05:51: 28000000 INFO @ Tue, 16 Jun 2020 09:05:51: #2 number of paired peaks: 36 WARNING @ Tue, 16 Jun 2020 09:05:51: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:05:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3583338/SRX3583338.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583338/SRX3583338.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583338/SRX3583338.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583338/SRX3583338.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:05:52: #2 number of paired peaks: 36 WARNING @ Tue, 16 Jun 2020 09:05:52: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:05:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3583338/SRX3583338.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583338/SRX3583338.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583338/SRX3583338.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583338/SRX3583338.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:05:57: 29000000 INFO @ Tue, 16 Jun 2020 09:06:04: 30000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:06:11: 31000000 INFO @ Tue, 16 Jun 2020 09:06:12: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:06:12: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:06:12: #1 total tags in treatment: 31167909 INFO @ Tue, 16 Jun 2020 09:06:12: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:06:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:06:12: #1 tags after filtering in treatment: 31167909 INFO @ Tue, 16 Jun 2020 09:06:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:06:12: #1 finished! INFO @ Tue, 16 Jun 2020 09:06:12: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:06:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:06:14: #2 number of paired peaks: 36 WARNING @ Tue, 16 Jun 2020 09:06:14: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:06:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3583338/SRX3583338.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583338/SRX3583338.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583338/SRX3583338.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583338/SRX3583338.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling