Job ID = 6367593 SRX = SRX331350 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:35:05 prefetch.2.10.7: 1) Downloading 'SRR947587'... 2020-06-15T23:35:05 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:45:09 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:45:09 prefetch.2.10.7: 1) 'SRR947587' was downloaded successfully Read 47950615 spots for SRR947587/SRR947587.sra Written 47950615 spots for SRR947587/SRR947587.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:31 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3138626 (6.55%) aligned 0 times 37486006 (78.18%) aligned exactly 1 time 7325983 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:10:31 Overall time: 00:10:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8189339 / 44811989 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:08:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331350/SRX331350.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331350/SRX331350.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331350/SRX331350.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331350/SRX331350.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:08:54: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:08:54: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:09:02: 1000000 INFO @ Tue, 16 Jun 2020 09:09:11: 2000000 INFO @ Tue, 16 Jun 2020 09:09:18: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:09:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331350/SRX331350.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331350/SRX331350.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331350/SRX331350.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331350/SRX331350.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:09:24: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:09:24: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:09:26: 4000000 INFO @ Tue, 16 Jun 2020 09:09:30: 1000000 INFO @ Tue, 16 Jun 2020 09:09:34: 5000000 INFO @ Tue, 16 Jun 2020 09:09:36: 2000000 INFO @ Tue, 16 Jun 2020 09:09:41: 3000000 INFO @ Tue, 16 Jun 2020 09:09:41: 6000000 INFO @ Tue, 16 Jun 2020 09:09:47: 4000000 INFO @ Tue, 16 Jun 2020 09:09:49: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:09:53: 5000000 INFO @ Tue, 16 Jun 2020 09:09:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331350/SRX331350.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331350/SRX331350.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331350/SRX331350.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331350/SRX331350.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:09:54: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:09:54: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:09:57: 8000000 INFO @ Tue, 16 Jun 2020 09:09:59: 6000000 INFO @ Tue, 16 Jun 2020 09:10:01: 1000000 INFO @ Tue, 16 Jun 2020 09:10:05: 7000000 INFO @ Tue, 16 Jun 2020 09:10:05: 9000000 INFO @ Tue, 16 Jun 2020 09:10:08: 2000000 INFO @ Tue, 16 Jun 2020 09:10:11: 8000000 INFO @ Tue, 16 Jun 2020 09:10:13: 10000000 INFO @ Tue, 16 Jun 2020 09:10:15: 3000000 INFO @ Tue, 16 Jun 2020 09:10:17: 9000000 INFO @ Tue, 16 Jun 2020 09:10:21: 11000000 INFO @ Tue, 16 Jun 2020 09:10:21: 4000000 INFO @ Tue, 16 Jun 2020 09:10:23: 10000000 INFO @ Tue, 16 Jun 2020 09:10:28: 5000000 INFO @ Tue, 16 Jun 2020 09:10:29: 11000000 INFO @ Tue, 16 Jun 2020 09:10:29: 12000000 INFO @ Tue, 16 Jun 2020 09:10:35: 12000000 INFO @ Tue, 16 Jun 2020 09:10:35: 6000000 INFO @ Tue, 16 Jun 2020 09:10:37: 13000000 INFO @ Tue, 16 Jun 2020 09:10:41: 13000000 INFO @ Tue, 16 Jun 2020 09:10:42: 7000000 INFO @ Tue, 16 Jun 2020 09:10:46: 14000000 INFO @ Tue, 16 Jun 2020 09:10:47: 14000000 INFO @ Tue, 16 Jun 2020 09:10:49: 8000000 INFO @ Tue, 16 Jun 2020 09:10:54: 15000000 INFO @ Tue, 16 Jun 2020 09:10:55: 15000000 INFO @ Tue, 16 Jun 2020 09:10:55: 9000000 INFO @ Tue, 16 Jun 2020 09:11:00: 16000000 INFO @ Tue, 16 Jun 2020 09:11:02: 10000000 INFO @ Tue, 16 Jun 2020 09:11:03: 16000000 INFO @ Tue, 16 Jun 2020 09:11:06: 17000000 INFO @ Tue, 16 Jun 2020 09:11:09: 11000000 INFO @ Tue, 16 Jun 2020 09:11:12: 17000000 INFO @ Tue, 16 Jun 2020 09:11:12: 18000000 INFO @ Tue, 16 Jun 2020 09:11:16: 12000000 INFO @ Tue, 16 Jun 2020 09:11:18: 19000000 INFO @ Tue, 16 Jun 2020 09:11:20: 18000000 INFO @ Tue, 16 Jun 2020 09:11:23: 13000000 INFO @ Tue, 16 Jun 2020 09:11:24: 20000000 INFO @ Tue, 16 Jun 2020 09:11:27: 19000000 INFO @ Tue, 16 Jun 2020 09:11:30: 14000000 INFO @ Tue, 16 Jun 2020 09:11:30: 21000000 INFO @ Tue, 16 Jun 2020 09:11:34: 20000000 INFO @ Tue, 16 Jun 2020 09:11:36: 22000000 INFO @ Tue, 16 Jun 2020 09:11:37: 15000000 INFO @ Tue, 16 Jun 2020 09:11:41: 21000000 INFO @ Tue, 16 Jun 2020 09:11:42: 23000000 INFO @ Tue, 16 Jun 2020 09:11:44: 16000000 INFO @ Tue, 16 Jun 2020 09:11:49: 24000000 INFO @ Tue, 16 Jun 2020 09:11:49: 22000000 INFO @ Tue, 16 Jun 2020 09:11:51: 17000000 INFO @ Tue, 16 Jun 2020 09:11:55: 25000000 INFO @ Tue, 16 Jun 2020 09:11:56: 23000000 INFO @ Tue, 16 Jun 2020 09:11:58: 18000000 INFO @ Tue, 16 Jun 2020 09:12:01: 26000000 INFO @ Tue, 16 Jun 2020 09:12:03: 24000000 INFO @ Tue, 16 Jun 2020 09:12:05: 19000000 INFO @ Tue, 16 Jun 2020 09:12:07: 27000000 INFO @ Tue, 16 Jun 2020 09:12:11: 25000000 INFO @ Tue, 16 Jun 2020 09:12:12: 20000000 INFO @ Tue, 16 Jun 2020 09:12:13: 28000000 INFO @ Tue, 16 Jun 2020 09:12:18: 26000000 INFO @ Tue, 16 Jun 2020 09:12:18: 21000000 INFO @ Tue, 16 Jun 2020 09:12:20: 29000000 INFO @ Tue, 16 Jun 2020 09:12:25: 27000000 INFO @ Tue, 16 Jun 2020 09:12:25: 22000000 INFO @ Tue, 16 Jun 2020 09:12:26: 30000000 INFO @ Tue, 16 Jun 2020 09:12:32: 23000000 INFO @ Tue, 16 Jun 2020 09:12:32: 31000000 INFO @ Tue, 16 Jun 2020 09:12:32: 28000000 INFO @ Tue, 16 Jun 2020 09:12:38: 32000000 INFO @ Tue, 16 Jun 2020 09:12:38: 24000000 INFO @ Tue, 16 Jun 2020 09:12:39: 29000000 INFO @ Tue, 16 Jun 2020 09:12:45: 33000000 INFO @ Tue, 16 Jun 2020 09:12:45: 25000000 INFO @ Tue, 16 Jun 2020 09:12:46: 30000000 INFO @ Tue, 16 Jun 2020 09:12:51: 34000000 INFO @ Tue, 16 Jun 2020 09:12:52: 26000000 INFO @ Tue, 16 Jun 2020 09:12:52: 31000000 INFO @ Tue, 16 Jun 2020 09:12:57: 35000000 INFO @ Tue, 16 Jun 2020 09:12:58: 27000000 INFO @ Tue, 16 Jun 2020 09:12:59: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:13:03: 36000000 INFO @ Tue, 16 Jun 2020 09:13:04: 28000000 INFO @ Tue, 16 Jun 2020 09:13:06: 33000000 INFO @ Tue, 16 Jun 2020 09:13:07: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:13:07: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:13:07: #1 total tags in treatment: 36622650 INFO @ Tue, 16 Jun 2020 09:13:07: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:13:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:13:08: #1 tags after filtering in treatment: 36622650 INFO @ Tue, 16 Jun 2020 09:13:08: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:13:08: #1 finished! INFO @ Tue, 16 Jun 2020 09:13:08: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:13:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:13:10: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:13:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:13:10: Process for pairing-model is terminated! INFO @ Tue, 16 Jun 2020 09:13:11: 29000000 cut: /home/okishinya/chipatlas/results/ce11/SRX331350/SRX331350.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX331350/SRX331350.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX331350/SRX331350.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX331350/SRX331350.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:13:12: 34000000 INFO @ Tue, 16 Jun 2020 09:13:17: 30000000 INFO @ Tue, 16 Jun 2020 09:13:19: 35000000 INFO @ Tue, 16 Jun 2020 09:13:23: 31000000 INFO @ Tue, 16 Jun 2020 09:13:25: 36000000 INFO @ Tue, 16 Jun 2020 09:13:29: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:13:29: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:13:29: #1 total tags in treatment: 36622650 INFO @ Tue, 16 Jun 2020 09:13:29: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:13:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:13:29: 32000000 INFO @ Tue, 16 Jun 2020 09:13:30: #1 tags after filtering in treatment: 36622650 INFO @ Tue, 16 Jun 2020 09:13:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:13:30: #1 finished! INFO @ Tue, 16 Jun 2020 09:13:30: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:13:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:13:32: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:13:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:13:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX331350/SRX331350.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX331350/SRX331350.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX331350/SRX331350.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX331350/SRX331350.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:13:36: 33000000 INFO @ Tue, 16 Jun 2020 09:13:42: 34000000 INFO @ Tue, 16 Jun 2020 09:13:48: 35000000 INFO @ Tue, 16 Jun 2020 09:13:54: 36000000 INFO @ Tue, 16 Jun 2020 09:13:58: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:13:58: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:13:58: #1 total tags in treatment: 36622650 INFO @ Tue, 16 Jun 2020 09:13:58: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:13:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:13:58: #1 tags after filtering in treatment: 36622650 INFO @ Tue, 16 Jun 2020 09:13:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:13:58: #1 finished! INFO @ Tue, 16 Jun 2020 09:13:58: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:13:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:14:01: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:14:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:14:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX331350/SRX331350.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX331350/SRX331350.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX331350/SRX331350.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX331350/SRX331350.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。