Job ID = 6367574 SRX = SRX331330 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:31:35 prefetch.2.10.7: 1) Downloading 'SRR947567'... 2020-06-15T23:31:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:32:02 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:32:03 prefetch.2.10.7: 'SRR947567' is valid 2020-06-15T23:32:03 prefetch.2.10.7: 1) 'SRR947567' was downloaded successfully Read 2303347 spots for SRR947567/SRR947567.sra Written 2303347 spots for SRR947567/SRR947567.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:19 2303347 reads; of these: 2303347 (100.00%) were unpaired; of these: 682489 (29.63%) aligned 0 times 1424457 (61.84%) aligned exactly 1 time 196401 (8.53%) aligned >1 times 70.37% overall alignment rate Time searching: 00:00:19 Overall time: 00:00:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 180653 / 1620858 = 0.1115 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:33:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331330/SRX331330.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331330/SRX331330.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331330/SRX331330.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331330/SRX331330.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:33:16: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:33:16: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:33:22: 1000000 INFO @ Tue, 16 Jun 2020 08:33:24: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:33:24: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:33:24: #1 total tags in treatment: 1440205 INFO @ Tue, 16 Jun 2020 08:33:24: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:33:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:33:24: #1 tags after filtering in treatment: 1440205 INFO @ Tue, 16 Jun 2020 08:33:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:33:24: #1 finished! INFO @ Tue, 16 Jun 2020 08:33:24: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:33:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:33:24: #2 number of paired peaks: 1935 INFO @ Tue, 16 Jun 2020 08:33:24: start model_add_line... INFO @ Tue, 16 Jun 2020 08:33:24: start X-correlation... INFO @ Tue, 16 Jun 2020 08:33:24: end of X-cor INFO @ Tue, 16 Jun 2020 08:33:24: #2 finished! INFO @ Tue, 16 Jun 2020 08:33:24: #2 predicted fragment length is 157 bps INFO @ Tue, 16 Jun 2020 08:33:24: #2 alternative fragment length(s) may be 157 bps INFO @ Tue, 16 Jun 2020 08:33:24: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX331330/SRX331330.05_model.r INFO @ Tue, 16 Jun 2020 08:33:24: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:33:24: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:33:28: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:33:30: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX331330/SRX331330.05_peaks.xls INFO @ Tue, 16 Jun 2020 08:33:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX331330/SRX331330.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:33:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX331330/SRX331330.05_summits.bed INFO @ Tue, 16 Jun 2020 08:33:30: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (2715 records, 4 fields): 4 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:33:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331330/SRX331330.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331330/SRX331330.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331330/SRX331330.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331330/SRX331330.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:33:47: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:33:47: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:33:53: 1000000 INFO @ Tue, 16 Jun 2020 08:33:56: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:33:56: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:33:56: #1 total tags in treatment: 1440205 INFO @ Tue, 16 Jun 2020 08:33:56: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:33:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:33:56: #1 tags after filtering in treatment: 1440205 INFO @ Tue, 16 Jun 2020 08:33:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:33:56: #1 finished! INFO @ Tue, 16 Jun 2020 08:33:56: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:33:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:33:56: #2 number of paired peaks: 1935 INFO @ Tue, 16 Jun 2020 08:33:56: start model_add_line... INFO @ Tue, 16 Jun 2020 08:33:56: start X-correlation... INFO @ Tue, 16 Jun 2020 08:33:56: end of X-cor INFO @ Tue, 16 Jun 2020 08:33:56: #2 finished! INFO @ Tue, 16 Jun 2020 08:33:56: #2 predicted fragment length is 157 bps INFO @ Tue, 16 Jun 2020 08:33:56: #2 alternative fragment length(s) may be 157 bps INFO @ Tue, 16 Jun 2020 08:33:56: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX331330/SRX331330.10_model.r INFO @ Tue, 16 Jun 2020 08:33:56: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:33:56: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:33:59: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:34:01: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX331330/SRX331330.10_peaks.xls INFO @ Tue, 16 Jun 2020 08:34:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX331330/SRX331330.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:34:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX331330/SRX331330.10_summits.bed INFO @ Tue, 16 Jun 2020 08:34:01: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1268 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:34:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331330/SRX331330.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331330/SRX331330.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331330/SRX331330.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331330/SRX331330.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:34:16: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:34:16: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:34:21: 1000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:34:24: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:34:24: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:34:24: #1 total tags in treatment: 1440205 INFO @ Tue, 16 Jun 2020 08:34:24: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:34:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:34:24: #1 tags after filtering in treatment: 1440205 INFO @ Tue, 16 Jun 2020 08:34:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:34:24: #1 finished! INFO @ Tue, 16 Jun 2020 08:34:24: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:34:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:34:24: #2 number of paired peaks: 1935 INFO @ Tue, 16 Jun 2020 08:34:24: start model_add_line... INFO @ Tue, 16 Jun 2020 08:34:24: start X-correlation... INFO @ Tue, 16 Jun 2020 08:34:24: end of X-cor INFO @ Tue, 16 Jun 2020 08:34:24: #2 finished! INFO @ Tue, 16 Jun 2020 08:34:24: #2 predicted fragment length is 157 bps INFO @ Tue, 16 Jun 2020 08:34:24: #2 alternative fragment length(s) may be 157 bps INFO @ Tue, 16 Jun 2020 08:34:24: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX331330/SRX331330.20_model.r INFO @ Tue, 16 Jun 2020 08:34:24: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:34:24: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:34:27: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:34:29: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX331330/SRX331330.20_peaks.xls INFO @ Tue, 16 Jun 2020 08:34:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX331330/SRX331330.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:34:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX331330/SRX331330.20_summits.bed INFO @ Tue, 16 Jun 2020 08:34:29: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (435 records, 4 fields): 1 millis CompletedMACS2peakCalling