Job ID = 6367563 SRX = SRX331319 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:29:35 prefetch.2.10.7: 1) Downloading 'SRR947556'... 2020-06-15T23:29:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:30:00 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:30:00 prefetch.2.10.7: 'SRR947556' is valid 2020-06-15T23:30:00 prefetch.2.10.7: 1) 'SRR947556' was downloaded successfully Read 3945897 spots for SRR947556/SRR947556.sra Written 3945897 spots for SRR947556/SRR947556.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:39 3945897 reads; of these: 3945897 (100.00%) were unpaired; of these: 17584 (0.45%) aligned 0 times 3253472 (82.45%) aligned exactly 1 time 674841 (17.10%) aligned >1 times 99.55% overall alignment rate Time searching: 00:00:39 Overall time: 00:00:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 246697 / 3928313 = 0.0628 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:32:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331319/SRX331319.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331319/SRX331319.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331319/SRX331319.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331319/SRX331319.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:32:05: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:32:05: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:32:09: 1000000 INFO @ Tue, 16 Jun 2020 08:32:14: 2000000 INFO @ Tue, 16 Jun 2020 08:32:18: 3000000 INFO @ Tue, 16 Jun 2020 08:32:21: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:32:21: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:32:21: #1 total tags in treatment: 3681616 INFO @ Tue, 16 Jun 2020 08:32:21: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:32:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:32:21: #1 tags after filtering in treatment: 3681616 INFO @ Tue, 16 Jun 2020 08:32:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:32:21: #1 finished! INFO @ Tue, 16 Jun 2020 08:32:21: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:32:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:32:22: #2 number of paired peaks: 375 WARNING @ Tue, 16 Jun 2020 08:32:22: Fewer paired peaks (375) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 375 pairs to build model! INFO @ Tue, 16 Jun 2020 08:32:22: start model_add_line... INFO @ Tue, 16 Jun 2020 08:32:22: start X-correlation... INFO @ Tue, 16 Jun 2020 08:32:22: end of X-cor INFO @ Tue, 16 Jun 2020 08:32:22: #2 finished! INFO @ Tue, 16 Jun 2020 08:32:22: #2 predicted fragment length is 36 bps INFO @ Tue, 16 Jun 2020 08:32:22: #2 alternative fragment length(s) may be 4,36,591 bps INFO @ Tue, 16 Jun 2020 08:32:22: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX331319/SRX331319.05_model.r WARNING @ Tue, 16 Jun 2020 08:32:22: #2 Since the d (36) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:32:22: #2 You may need to consider one of the other alternative d(s): 4,36,591 WARNING @ Tue, 16 Jun 2020 08:32:22: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:32:22: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:32:22: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:32:29: #3 Call peaks for each chromosome... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:32:33: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX331319/SRX331319.05_peaks.xls INFO @ Tue, 16 Jun 2020 08:32:33: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX331319/SRX331319.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:32:33: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX331319/SRX331319.05_summits.bed INFO @ Tue, 16 Jun 2020 08:32:33: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (369 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:32:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331319/SRX331319.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331319/SRX331319.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331319/SRX331319.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331319/SRX331319.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:32:35: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:32:35: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:32:39: 1000000 INFO @ Tue, 16 Jun 2020 08:32:44: 2000000 INFO @ Tue, 16 Jun 2020 08:32:48: 3000000 INFO @ Tue, 16 Jun 2020 08:32:51: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:32:51: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:32:51: #1 total tags in treatment: 3681616 INFO @ Tue, 16 Jun 2020 08:32:51: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:32:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:32:51: #1 tags after filtering in treatment: 3681616 INFO @ Tue, 16 Jun 2020 08:32:51: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:32:51: #1 finished! INFO @ Tue, 16 Jun 2020 08:32:51: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:32:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:32:52: #2 number of paired peaks: 375 WARNING @ Tue, 16 Jun 2020 08:32:52: Fewer paired peaks (375) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 375 pairs to build model! INFO @ Tue, 16 Jun 2020 08:32:52: start model_add_line... INFO @ Tue, 16 Jun 2020 08:32:52: start X-correlation... INFO @ Tue, 16 Jun 2020 08:32:52: end of X-cor INFO @ Tue, 16 Jun 2020 08:32:52: #2 finished! INFO @ Tue, 16 Jun 2020 08:32:52: #2 predicted fragment length is 36 bps INFO @ Tue, 16 Jun 2020 08:32:52: #2 alternative fragment length(s) may be 4,36,591 bps INFO @ Tue, 16 Jun 2020 08:32:52: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX331319/SRX331319.10_model.r WARNING @ Tue, 16 Jun 2020 08:32:52: #2 Since the d (36) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:32:52: #2 You may need to consider one of the other alternative d(s): 4,36,591 WARNING @ Tue, 16 Jun 2020 08:32:52: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:32:52: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:32:52: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:32:59: #3 Call peaks for each chromosome... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:33:03: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX331319/SRX331319.10_peaks.xls INFO @ Tue, 16 Jun 2020 08:33:03: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX331319/SRX331319.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:33:03: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX331319/SRX331319.10_summits.bed INFO @ Tue, 16 Jun 2020 08:33:03: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (190 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:33:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331319/SRX331319.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331319/SRX331319.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331319/SRX331319.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331319/SRX331319.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:33:05: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:33:05: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:33:10: 1000000 INFO @ Tue, 16 Jun 2020 08:33:14: 2000000 INFO @ Tue, 16 Jun 2020 08:33:19: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:33:22: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:33:22: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:33:22: #1 total tags in treatment: 3681616 INFO @ Tue, 16 Jun 2020 08:33:22: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:33:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:33:22: #1 tags after filtering in treatment: 3681616 INFO @ Tue, 16 Jun 2020 08:33:22: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:33:22: #1 finished! INFO @ Tue, 16 Jun 2020 08:33:22: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:33:22: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:33:22: #2 number of paired peaks: 375 WARNING @ Tue, 16 Jun 2020 08:33:22: Fewer paired peaks (375) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 375 pairs to build model! INFO @ Tue, 16 Jun 2020 08:33:22: start model_add_line... INFO @ Tue, 16 Jun 2020 08:33:23: start X-correlation... INFO @ Tue, 16 Jun 2020 08:33:23: end of X-cor INFO @ Tue, 16 Jun 2020 08:33:23: #2 finished! INFO @ Tue, 16 Jun 2020 08:33:23: #2 predicted fragment length is 36 bps INFO @ Tue, 16 Jun 2020 08:33:23: #2 alternative fragment length(s) may be 4,36,591 bps INFO @ Tue, 16 Jun 2020 08:33:23: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX331319/SRX331319.20_model.r WARNING @ Tue, 16 Jun 2020 08:33:23: #2 Since the d (36) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:33:23: #2 You may need to consider one of the other alternative d(s): 4,36,591 WARNING @ Tue, 16 Jun 2020 08:33:23: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:33:23: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:33:23: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:33:30: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:33:35: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX331319/SRX331319.20_peaks.xls INFO @ Tue, 16 Jun 2020 08:33:35: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX331319/SRX331319.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:33:35: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX331319/SRX331319.20_summits.bed INFO @ Tue, 16 Jun 2020 08:33:35: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (69 records, 4 fields): 1 millis CompletedMACS2peakCalling