Job ID = 6367543 SRX = SRX331299 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:34:20 prefetch.2.10.7: 1) Downloading 'SRR947536'... 2020-06-15T23:34:20 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:34:58 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:34:59 prefetch.2.10.7: 'SRR947536' is valid 2020-06-15T23:34:59 prefetch.2.10.7: 1) 'SRR947536' was downloaded successfully Read 5936104 spots for SRR947536/SRR947536.sra Written 5936104 spots for SRR947536/SRR947536.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:01 5936104 reads; of these: 5936104 (100.00%) were unpaired; of these: 80076 (1.35%) aligned 0 times 4970325 (83.73%) aligned exactly 1 time 885703 (14.92%) aligned >1 times 98.65% overall alignment rate Time searching: 00:01:01 Overall time: 00:01:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 645044 / 5856028 = 0.1102 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:37:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331299/SRX331299.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331299/SRX331299.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331299/SRX331299.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331299/SRX331299.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:37:49: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:37:49: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:37:55: 1000000 INFO @ Tue, 16 Jun 2020 08:38:01: 2000000 INFO @ Tue, 16 Jun 2020 08:38:07: 3000000 INFO @ Tue, 16 Jun 2020 08:38:13: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:38:19: 5000000 INFO @ Tue, 16 Jun 2020 08:38:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331299/SRX331299.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331299/SRX331299.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331299/SRX331299.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331299/SRX331299.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:38:19: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:38:19: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:38:20: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:38:20: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:38:20: #1 total tags in treatment: 5210984 INFO @ Tue, 16 Jun 2020 08:38:20: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:38:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:38:20: #1 tags after filtering in treatment: 5210984 INFO @ Tue, 16 Jun 2020 08:38:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:38:20: #1 finished! INFO @ Tue, 16 Jun 2020 08:38:20: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:38:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:38:20: #2 number of paired peaks: 373 WARNING @ Tue, 16 Jun 2020 08:38:20: Fewer paired peaks (373) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 373 pairs to build model! INFO @ Tue, 16 Jun 2020 08:38:20: start model_add_line... INFO @ Tue, 16 Jun 2020 08:38:20: start X-correlation... INFO @ Tue, 16 Jun 2020 08:38:20: end of X-cor INFO @ Tue, 16 Jun 2020 08:38:20: #2 finished! INFO @ Tue, 16 Jun 2020 08:38:20: #2 predicted fragment length is 34 bps INFO @ Tue, 16 Jun 2020 08:38:20: #2 alternative fragment length(s) may be 3,34,554 bps INFO @ Tue, 16 Jun 2020 08:38:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX331299/SRX331299.05_model.r WARNING @ Tue, 16 Jun 2020 08:38:20: #2 Since the d (34) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:38:20: #2 You may need to consider one of the other alternative d(s): 3,34,554 WARNING @ Tue, 16 Jun 2020 08:38:20: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:38:20: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:38:20: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:38:24: 1000000 INFO @ Tue, 16 Jun 2020 08:38:29: 2000000 INFO @ Tue, 16 Jun 2020 08:38:32: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:38:34: 3000000 INFO @ Tue, 16 Jun 2020 08:38:38: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX331299/SRX331299.05_peaks.xls INFO @ Tue, 16 Jun 2020 08:38:38: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX331299/SRX331299.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:38:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX331299/SRX331299.05_summits.bed INFO @ Tue, 16 Jun 2020 08:38:38: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (400 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:38:39: 4000000 INFO @ Tue, 16 Jun 2020 08:38:43: 5000000 INFO @ Tue, 16 Jun 2020 08:38:44: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:38:44: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:38:44: #1 total tags in treatment: 5210984 INFO @ Tue, 16 Jun 2020 08:38:44: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:38:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:38:44: #1 tags after filtering in treatment: 5210984 INFO @ Tue, 16 Jun 2020 08:38:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:38:44: #1 finished! INFO @ Tue, 16 Jun 2020 08:38:44: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:38:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:38:45: #2 number of paired peaks: 373 WARNING @ Tue, 16 Jun 2020 08:38:45: Fewer paired peaks (373) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 373 pairs to build model! INFO @ Tue, 16 Jun 2020 08:38:45: start model_add_line... INFO @ Tue, 16 Jun 2020 08:38:45: start X-correlation... INFO @ Tue, 16 Jun 2020 08:38:45: end of X-cor INFO @ Tue, 16 Jun 2020 08:38:45: #2 finished! INFO @ Tue, 16 Jun 2020 08:38:45: #2 predicted fragment length is 34 bps INFO @ Tue, 16 Jun 2020 08:38:45: #2 alternative fragment length(s) may be 3,34,554 bps INFO @ Tue, 16 Jun 2020 08:38:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX331299/SRX331299.10_model.r WARNING @ Tue, 16 Jun 2020 08:38:45: #2 Since the d (34) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:38:45: #2 You may need to consider one of the other alternative d(s): 3,34,554 WARNING @ Tue, 16 Jun 2020 08:38:45: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:38:45: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:38:45: #3 Pre-compute pvalue-qvalue table... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:38:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331299/SRX331299.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331299/SRX331299.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331299/SRX331299.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331299/SRX331299.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:38:49: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:38:49: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:38:55: 1000000 INFO @ Tue, 16 Jun 2020 08:38:56: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:39:01: 2000000 INFO @ Tue, 16 Jun 2020 08:39:01: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX331299/SRX331299.10_peaks.xls INFO @ Tue, 16 Jun 2020 08:39:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX331299/SRX331299.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:39:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX331299/SRX331299.10_summits.bed INFO @ Tue, 16 Jun 2020 08:39:01: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (173 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:39:07: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:39:13: 4000000 INFO @ Tue, 16 Jun 2020 08:39:19: 5000000 INFO @ Tue, 16 Jun 2020 08:39:20: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:39:20: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:39:20: #1 total tags in treatment: 5210984 INFO @ Tue, 16 Jun 2020 08:39:20: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:39:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:39:20: #1 tags after filtering in treatment: 5210984 INFO @ Tue, 16 Jun 2020 08:39:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:39:20: #1 finished! INFO @ Tue, 16 Jun 2020 08:39:20: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:39:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:39:20: #2 number of paired peaks: 373 WARNING @ Tue, 16 Jun 2020 08:39:20: Fewer paired peaks (373) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 373 pairs to build model! INFO @ Tue, 16 Jun 2020 08:39:20: start model_add_line... INFO @ Tue, 16 Jun 2020 08:39:20: start X-correlation... INFO @ Tue, 16 Jun 2020 08:39:20: end of X-cor INFO @ Tue, 16 Jun 2020 08:39:20: #2 finished! INFO @ Tue, 16 Jun 2020 08:39:20: #2 predicted fragment length is 34 bps INFO @ Tue, 16 Jun 2020 08:39:20: #2 alternative fragment length(s) may be 3,34,554 bps INFO @ Tue, 16 Jun 2020 08:39:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX331299/SRX331299.20_model.r WARNING @ Tue, 16 Jun 2020 08:39:20: #2 Since the d (34) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:39:20: #2 You may need to consider one of the other alternative d(s): 3,34,554 WARNING @ Tue, 16 Jun 2020 08:39:20: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:39:20: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:39:20: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:39:32: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:39:37: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX331299/SRX331299.20_peaks.xls INFO @ Tue, 16 Jun 2020 08:39:37: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX331299/SRX331299.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:39:37: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX331299/SRX331299.20_summits.bed INFO @ Tue, 16 Jun 2020 08:39:37: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (60 records, 4 fields): 1 millis CompletedMACS2peakCalling