Job ID = 6367533 SRX = SRX331289 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:29:50 prefetch.2.10.7: 1) Downloading 'SRR947526'... 2020-06-15T23:29:50 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:30:55 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:30:55 prefetch.2.10.7: 'SRR947526' is valid 2020-06-15T23:30:55 prefetch.2.10.7: 1) 'SRR947526' was downloaded successfully Read 5945921 spots for SRR947526/SRR947526.sra Written 5945921 spots for SRR947526/SRR947526.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:03 5945921 reads; of these: 5945921 (100.00%) were unpaired; of these: 229924 (3.87%) aligned 0 times 4732600 (79.59%) aligned exactly 1 time 983397 (16.54%) aligned >1 times 96.13% overall alignment rate Time searching: 00:01:03 Overall time: 00:01:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 271793 / 5715997 = 0.0475 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:33:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331289/SRX331289.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331289/SRX331289.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331289/SRX331289.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331289/SRX331289.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:33:50: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:33:50: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:33:54: 1000000 INFO @ Tue, 16 Jun 2020 08:33:59: 2000000 INFO @ Tue, 16 Jun 2020 08:34:03: 3000000 INFO @ Tue, 16 Jun 2020 08:34:08: 4000000 INFO @ Tue, 16 Jun 2020 08:34:12: 5000000 INFO @ Tue, 16 Jun 2020 08:34:14: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:34:14: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:34:14: #1 total tags in treatment: 5444204 INFO @ Tue, 16 Jun 2020 08:34:14: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:34:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:34:14: #1 tags after filtering in treatment: 5444204 INFO @ Tue, 16 Jun 2020 08:34:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:34:14: #1 finished! INFO @ Tue, 16 Jun 2020 08:34:14: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:34:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:34:15: #2 number of paired peaks: 346 WARNING @ Tue, 16 Jun 2020 08:34:15: Fewer paired peaks (346) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 346 pairs to build model! INFO @ Tue, 16 Jun 2020 08:34:15: start model_add_line... INFO @ Tue, 16 Jun 2020 08:34:15: start X-correlation... INFO @ Tue, 16 Jun 2020 08:34:15: end of X-cor INFO @ Tue, 16 Jun 2020 08:34:15: #2 finished! INFO @ Tue, 16 Jun 2020 08:34:15: #2 predicted fragment length is 33 bps INFO @ Tue, 16 Jun 2020 08:34:15: #2 alternative fragment length(s) may be 4,33,501 bps INFO @ Tue, 16 Jun 2020 08:34:15: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX331289/SRX331289.05_model.r WARNING @ Tue, 16 Jun 2020 08:34:15: #2 Since the d (33) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:34:15: #2 You may need to consider one of the other alternative d(s): 4,33,501 WARNING @ Tue, 16 Jun 2020 08:34:15: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:34:15: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:34:15: #3 Pre-compute pvalue-qvalue table... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:34:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331289/SRX331289.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331289/SRX331289.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331289/SRX331289.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331289/SRX331289.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:34:20: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:34:20: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:34:25: 1000000 INFO @ Tue, 16 Jun 2020 08:34:26: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:34:30: 2000000 INFO @ Tue, 16 Jun 2020 08:34:32: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX331289/SRX331289.05_peaks.xls INFO @ Tue, 16 Jun 2020 08:34:32: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX331289/SRX331289.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:34:32: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX331289/SRX331289.05_summits.bed INFO @ Tue, 16 Jun 2020 08:34:32: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (400 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:34:35: 3000000 INFO @ Tue, 16 Jun 2020 08:34:40: 4000000 INFO @ Tue, 16 Jun 2020 08:34:44: 5000000 INFO @ Tue, 16 Jun 2020 08:34:47: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:34:47: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:34:47: #1 total tags in treatment: 5444204 INFO @ Tue, 16 Jun 2020 08:34:47: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:34:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:34:47: #1 tags after filtering in treatment: 5444204 INFO @ Tue, 16 Jun 2020 08:34:47: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:34:47: #1 finished! INFO @ Tue, 16 Jun 2020 08:34:47: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:34:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:34:47: #2 number of paired peaks: 346 WARNING @ Tue, 16 Jun 2020 08:34:47: Fewer paired peaks (346) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 346 pairs to build model! INFO @ Tue, 16 Jun 2020 08:34:47: start model_add_line... INFO @ Tue, 16 Jun 2020 08:34:47: start X-correlation... INFO @ Tue, 16 Jun 2020 08:34:47: end of X-cor INFO @ Tue, 16 Jun 2020 08:34:47: #2 finished! INFO @ Tue, 16 Jun 2020 08:34:47: #2 predicted fragment length is 33 bps INFO @ Tue, 16 Jun 2020 08:34:47: #2 alternative fragment length(s) may be 4,33,501 bps INFO @ Tue, 16 Jun 2020 08:34:47: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX331289/SRX331289.10_model.r WARNING @ Tue, 16 Jun 2020 08:34:47: #2 Since the d (33) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:34:47: #2 You may need to consider one of the other alternative d(s): 4,33,501 WARNING @ Tue, 16 Jun 2020 08:34:47: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:34:47: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:34:47: #3 Pre-compute pvalue-qvalue table... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:34:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331289/SRX331289.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331289/SRX331289.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331289/SRX331289.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331289/SRX331289.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:34:50: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:34:50: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:34:54: 1000000 INFO @ Tue, 16 Jun 2020 08:34:59: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:34:59: 2000000 INFO @ Tue, 16 Jun 2020 08:35:03: 3000000 INFO @ Tue, 16 Jun 2020 08:35:05: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX331289/SRX331289.10_peaks.xls INFO @ Tue, 16 Jun 2020 08:35:05: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX331289/SRX331289.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:35:05: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX331289/SRX331289.10_summits.bed INFO @ Tue, 16 Jun 2020 08:35:05: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (226 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:35:08: 4000000 INFO @ Tue, 16 Jun 2020 08:35:12: 5000000 INFO @ Tue, 16 Jun 2020 08:35:14: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:35:14: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:35:14: #1 total tags in treatment: 5444204 INFO @ Tue, 16 Jun 2020 08:35:14: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:35:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:35:14: #1 tags after filtering in treatment: 5444204 INFO @ Tue, 16 Jun 2020 08:35:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:35:14: #1 finished! INFO @ Tue, 16 Jun 2020 08:35:14: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:35:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:35:15: #2 number of paired peaks: 346 WARNING @ Tue, 16 Jun 2020 08:35:15: Fewer paired peaks (346) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 346 pairs to build model! INFO @ Tue, 16 Jun 2020 08:35:15: start model_add_line... INFO @ Tue, 16 Jun 2020 08:35:15: start X-correlation... INFO @ Tue, 16 Jun 2020 08:35:15: end of X-cor INFO @ Tue, 16 Jun 2020 08:35:15: #2 finished! INFO @ Tue, 16 Jun 2020 08:35:15: #2 predicted fragment length is 33 bps INFO @ Tue, 16 Jun 2020 08:35:15: #2 alternative fragment length(s) may be 4,33,501 bps INFO @ Tue, 16 Jun 2020 08:35:15: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX331289/SRX331289.20_model.r WARNING @ Tue, 16 Jun 2020 08:35:15: #2 Since the d (33) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:35:15: #2 You may need to consider one of the other alternative d(s): 4,33,501 WARNING @ Tue, 16 Jun 2020 08:35:15: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:35:15: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:35:15: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:35:26: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:35:32: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX331289/SRX331289.20_peaks.xls INFO @ Tue, 16 Jun 2020 08:35:32: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX331289/SRX331289.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:35:32: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX331289/SRX331289.20_summits.bed INFO @ Tue, 16 Jun 2020 08:35:32: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (87 records, 4 fields): 1 millis CompletedMACS2peakCalling