Job ID = 6367416 SRX = SRX331175 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:28:06 prefetch.2.10.7: 1) Downloading 'SRR947407'... 2020-06-15T23:28:06 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:29:49 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:29:49 prefetch.2.10.7: 'SRR947407' is valid 2020-06-15T23:29:49 prefetch.2.10.7: 1) 'SRR947407' was downloaded successfully Read 8554421 spots for SRR947407/SRR947407.sra Written 8554421 spots for SRR947407/SRR947407.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:09 8554421 reads; of these: 8554421 (100.00%) were unpaired; of these: 2972630 (34.75%) aligned 0 times 4711300 (55.07%) aligned exactly 1 time 870491 (10.18%) aligned >1 times 65.25% overall alignment rate Time searching: 00:01:09 Overall time: 00:01:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 658448 / 5581791 = 0.1180 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:32:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331175/SRX331175.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331175/SRX331175.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331175/SRX331175.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331175/SRX331175.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:32:51: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:32:51: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:32:56: 1000000 INFO @ Tue, 16 Jun 2020 08:33:00: 2000000 INFO @ Tue, 16 Jun 2020 08:33:04: 3000000 INFO @ Tue, 16 Jun 2020 08:33:09: 4000000 INFO @ Tue, 16 Jun 2020 08:33:13: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:33:13: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:33:13: #1 total tags in treatment: 4923343 INFO @ Tue, 16 Jun 2020 08:33:13: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:33:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:33:13: #1 tags after filtering in treatment: 4923343 INFO @ Tue, 16 Jun 2020 08:33:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:33:13: #1 finished! INFO @ Tue, 16 Jun 2020 08:33:13: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:33:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:33:13: #2 number of paired peaks: 437 WARNING @ Tue, 16 Jun 2020 08:33:13: Fewer paired peaks (437) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 437 pairs to build model! INFO @ Tue, 16 Jun 2020 08:33:13: start model_add_line... INFO @ Tue, 16 Jun 2020 08:33:13: start X-correlation... INFO @ Tue, 16 Jun 2020 08:33:13: end of X-cor INFO @ Tue, 16 Jun 2020 08:33:13: #2 finished! INFO @ Tue, 16 Jun 2020 08:33:13: #2 predicted fragment length is 33 bps INFO @ Tue, 16 Jun 2020 08:33:13: #2 alternative fragment length(s) may be 3,33,593 bps INFO @ Tue, 16 Jun 2020 08:33:13: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX331175/SRX331175.05_model.r WARNING @ Tue, 16 Jun 2020 08:33:13: #2 Since the d (33) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:33:13: #2 You may need to consider one of the other alternative d(s): 3,33,593 WARNING @ Tue, 16 Jun 2020 08:33:13: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:33:13: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:33:13: #3 Pre-compute pvalue-qvalue table... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:33:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331175/SRX331175.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331175/SRX331175.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331175/SRX331175.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331175/SRX331175.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:33:21: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:33:21: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:33:23: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:33:25: 1000000 INFO @ Tue, 16 Jun 2020 08:33:28: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX331175/SRX331175.05_peaks.xls INFO @ Tue, 16 Jun 2020 08:33:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX331175/SRX331175.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:33:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX331175/SRX331175.05_summits.bed INFO @ Tue, 16 Jun 2020 08:33:28: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (441 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:33:30: 2000000 INFO @ Tue, 16 Jun 2020 08:33:34: 3000000 INFO @ Tue, 16 Jun 2020 08:33:38: 4000000 INFO @ Tue, 16 Jun 2020 08:33:42: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:33:42: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:33:42: #1 total tags in treatment: 4923343 INFO @ Tue, 16 Jun 2020 08:33:42: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:33:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:33:42: #1 tags after filtering in treatment: 4923343 INFO @ Tue, 16 Jun 2020 08:33:42: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:33:42: #1 finished! INFO @ Tue, 16 Jun 2020 08:33:42: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:33:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:33:43: #2 number of paired peaks: 437 WARNING @ Tue, 16 Jun 2020 08:33:43: Fewer paired peaks (437) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 437 pairs to build model! INFO @ Tue, 16 Jun 2020 08:33:43: start model_add_line... INFO @ Tue, 16 Jun 2020 08:33:43: start X-correlation... INFO @ Tue, 16 Jun 2020 08:33:43: end of X-cor INFO @ Tue, 16 Jun 2020 08:33:43: #2 finished! INFO @ Tue, 16 Jun 2020 08:33:43: #2 predicted fragment length is 33 bps INFO @ Tue, 16 Jun 2020 08:33:43: #2 alternative fragment length(s) may be 3,33,593 bps INFO @ Tue, 16 Jun 2020 08:33:43: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX331175/SRX331175.10_model.r WARNING @ Tue, 16 Jun 2020 08:33:43: #2 Since the d (33) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:33:43: #2 You may need to consider one of the other alternative d(s): 3,33,593 WARNING @ Tue, 16 Jun 2020 08:33:43: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:33:43: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:33:43: #3 Pre-compute pvalue-qvalue table... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:33:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331175/SRX331175.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331175/SRX331175.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331175/SRX331175.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331175/SRX331175.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:33:51: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:33:51: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:33:53: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:33:56: 1000000 INFO @ Tue, 16 Jun 2020 08:33:58: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX331175/SRX331175.10_peaks.xls INFO @ Tue, 16 Jun 2020 08:33:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX331175/SRX331175.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:33:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX331175/SRX331175.10_summits.bed INFO @ Tue, 16 Jun 2020 08:33:58: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (211 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:34:00: 2000000 INFO @ Tue, 16 Jun 2020 08:34:04: 3000000 INFO @ Tue, 16 Jun 2020 08:34:08: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:34:12: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:34:12: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:34:12: #1 total tags in treatment: 4923343 INFO @ Tue, 16 Jun 2020 08:34:12: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:34:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:34:12: #1 tags after filtering in treatment: 4923343 INFO @ Tue, 16 Jun 2020 08:34:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:34:12: #1 finished! INFO @ Tue, 16 Jun 2020 08:34:12: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:34:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:34:13: #2 number of paired peaks: 437 WARNING @ Tue, 16 Jun 2020 08:34:13: Fewer paired peaks (437) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 437 pairs to build model! INFO @ Tue, 16 Jun 2020 08:34:13: start model_add_line... INFO @ Tue, 16 Jun 2020 08:34:13: start X-correlation... INFO @ Tue, 16 Jun 2020 08:34:13: end of X-cor INFO @ Tue, 16 Jun 2020 08:34:13: #2 finished! INFO @ Tue, 16 Jun 2020 08:34:13: #2 predicted fragment length is 33 bps INFO @ Tue, 16 Jun 2020 08:34:13: #2 alternative fragment length(s) may be 3,33,593 bps INFO @ Tue, 16 Jun 2020 08:34:13: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX331175/SRX331175.20_model.r WARNING @ Tue, 16 Jun 2020 08:34:13: #2 Since the d (33) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:34:13: #2 You may need to consider one of the other alternative d(s): 3,33,593 WARNING @ Tue, 16 Jun 2020 08:34:13: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:34:13: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:34:13: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:34:23: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:34:28: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX331175/SRX331175.20_peaks.xls INFO @ Tue, 16 Jun 2020 08:34:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX331175/SRX331175.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:34:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX331175/SRX331175.20_summits.bed INFO @ Tue, 16 Jun 2020 08:34:28: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (47 records, 4 fields): 1 millis CompletedMACS2peakCalling